| 质粒名称: | mCherry-Rab11a-7 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 55124 |
| 存储实验室: | Michael Davidson |
|
载体骨架基本信息 |
|
| 载体名称: | mCherry |
| 载体出品公司: | Clontech |
| 空载体大小: | 4750 bp |
| 完整质粒大小: | 4989 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | Kanamycin, 50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Rab11a、NM_004663.3 |
| 物种来源: | H. sapiens (human) |
| 启动子: | CMV |
| 标签/融合蛋白: | mCherry (N terminal on backbone) |
| 克隆方法: | 未知 |
| 5’测序引物 | CMV-F |
| 3’测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | mCherry-Rab11a-7 was a gift from Michael Davidson (Addgene plasmid # 55124 ; http://n2t.net/addgene:55124 ; RRID:Addgene_55124) |
买家导航
mCherry-Rab11a-7载体质粒基本信息
mCherry-Rab11a-7质粒图谱载体图谱和mCherry-Rab11a-7载体序列质粒序列多克隆位点信息
mCherry-Rab11a-7质粒载体简介
mCherry-Rab11a-7质粒序列载体序列
LOCUS mCherry-Rab11a-7 5334 bp DNA circular SYN 14-APR-2025
DEFINITION Localization: Ras GTPase, Excitation: 587, Emission: 610.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5334)
TITLE Davidson lab - mCherry constructs
REFERENCE 2 (bases 1 to 5334)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"
COMMENT Sequence Label: mCherry-Rab11a-7
FEATURES Location/Qualifiers
source 1..5334
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 68..371
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 372..575
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 526..546
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 614..623
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 620..1327
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(765..783)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 1033..1052
/label=mCherry-F
/note="mCherry, forward primer"
polyA_signal 2129..2250
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2166..2185)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2220..2239
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2257..2712)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2394..2415)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2606..2625
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2739..2843
/gene="bla"
/label=AmpR promoter
promoter 2845..3202
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
enhancer 2845..3036
/label=SV40 enhancer
/note="enhancer for the SV40 early promoter (Herr, 1993)"
primer_bind complement(2868..2888)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3053..3188
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3237..4031
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 3237..4031
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3291..3310)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3901..3920
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4219..4238)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4263..4310
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4639..5227
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5128..5147
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
601 agcgctaccg gtcgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
661 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
721 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
781 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
841 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
901 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
961 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
1021 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
1081 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
1141 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
1201 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
1261 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
1321 gtacaagtcc ggactcagat ctcgagccat gggcacccgc gacgacgagt acgactacct
1381 ctttaaagtt gtccttattg gagattctgg tgttggaaag agtaatctcc tgtctcgatt
1441 tactcgaaat gagtttaatc tggaaagcaa gagcaccatt ggagtagagt ttgcaacaag
1501 aagcatccag gttgatggaa aaacaataaa ggcacagata tgggacacag cagggcaaga
1561 gcgatatcga gctataacat cagcatatta tcgtggagct gtaggtgcct tattggttta
1621 tgacattgct aaacatctca catatgaaaa tgtagagcga tggctgaaag aactgagaga
1681 tcatgctgat agtaacattg ttatcatgct tgtgggcaat aagagtgatc tacgtcatct
1741 cagggcagtt cctacagatg aagcaagagc ttttgcagaa aagaatggtt tgtcattcat
1801 tgaaacttcg gccctagact ctacaaatgt agaagctgct tttcagacaa ttttaacaga
1861 gatttaccgc attgtttctc agaagcaaat gtcagacaga cgcgaaaatg acatgtctcc
1921 aagcaacaat gtggttccta ttcatgttcc accaaccact gaaaacaagc caaaggtgca
1981 gtgctgtcag aacatctaag gatccaccgg atctagataa ctgatcataa tcagccatac
2041 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
2101 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
2161 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
2221 tggtttgtcc aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt
2281 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
2341 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
2401 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
2461 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
2521 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
2581 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
2641 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
2701 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
2761 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
2821 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca
2881 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
2941 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
3001 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
3061 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
3121 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
3181 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
3241 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
3301 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
3361 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
3421 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
3481 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
3541 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
3601 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
3661 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
3721 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
3781 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
3841 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
3901 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
3961 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
4021 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
4081 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
4141 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
4201 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
4261 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
4321 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
4381 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
4441 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
4501 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
4561 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
4621 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
4681 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
4741 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
4801 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
4861 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
4921 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
4981 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
5041 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
5101 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
5161 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
5221 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
5281 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgc
//
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