pUF1-dCas9-Sir2a

LOCUS       pUF1-dCas9-Sir2a       10972 bp ds-DNA     circular SYN 19-JUN-2019
DEFINITION  Combining with the specific sgRNA sequence to repress the Plasmodium
            falciparum endogenous gene transcription, through the decreasing of 
            H3K9 acetylation..
ACCESSION   .
VERSION     .
KEYWORDS    pUF1-dCas9-Sir2a
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10972)
  AUTHORS   Xiao B, Yin S, Hu Y, Sun M, Wei J, Huang Z, Wen Y, Dai X, Chen H, Mu
            J, Cui L, Jiang L
  TITLE     Epigenetic editing by CRISPR/dCas9 in Plasmodium falciparum.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Jan 2;116(1):255-260. doi: 
            10.1073/pnas.1813542116. Epub 2018 Dec 24.
  PUBMED    30584102
REFERENCE   2  (bases 1 to 10972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 19, 2019 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10972
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(1682..1729)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(1730..5830)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(5855..5875)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(5882..5947)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7435..7836
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MHAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT
                     GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPG
                     IKAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(8544..8563)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8663..8685
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8723..8741)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8809..8913
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8914..9774
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9132..9151)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9945..10533
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10434..10453
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10687..10704
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10821..10842
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10857..10887
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10895..10911
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10900..10922
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10919..10935
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10919..10935
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        10953..10971
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     10953..10970
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             10967..6
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ggccgcctac cctgaagaag aaaagtccga tgatgttgtg gacataaaga attgagctgt
       61 ctcaaaaaat gagaaaacaa acaaacaaac aaaataccat tatgcgcata tcctctgctc
      121 aatatcaatt tttctttatg ccagttttgt ttcgacacta tttgagcaac accttccctt
      181 tactatctac tactgttaca atgttatttt attcaccttt ttacaatttt atcaaaattt
      241 ttttgtatgc atataaatta tacattgtaa agccaaagtg tagataaaga acaccttgtt
      301 tatttttccc caattttttt tttttaccat ttttttatta accttgctca tttacctgct
      361 aatacgattg ctatatgata tcgaaattga aggaaaaaac atcatttgtg tttatatgtt
      421 tttttttaat ttttcaactt tttttattta actatgctaa tcatattata aacattttaa
      481 aaatcaagtc caactattta tgaatcattg aagagacaac ataattataa cagtaaaaat
      541 aataataata ataataattt tgtcttattg tttcaaacat attatgttcc ataaatatct
      601 aaatttttaa tatggtcata aattcatagg atttaattta tataaaatat ggctattcat
      661 actagccatt ttatgtgtgt aaagtttttt ttttctgaac aaaaaataaa atgttcataa
      721 ttttagctat ttacatgcat gtgcatgcac aaaaaaaaat atgcacacaa catacacatt
      781 tttacagtta taaatacaat caattggtat aaatatataa ataagaaaaa cgaacattaa
      841 gctgccatat ccttaattaa gtttcacttg acccttttaa tatatttgcc actttagtta
      901 attcactaaa ttttgtacat acatgataat cagacatttt gtttgttata tatgttttgg
      961 atatattaat ttccacaatt tttttttttt tcttacaagc gaaatgacac aaattagtag
     1021 ctgttgatac agtagacgat gtgccaatta ctaaaagtaa atcacatttt gctatttctt
     1081 cttctgcttc ttttaaaaga tctgatgaaa caacctctcc aaataatata atgtttggtt
     1141 tgaatattcc tccacaagga cattcaggtg gtaattgatg cataaaatga gatgttttct
     1201 gtaacatgat tttatttaat tttacaattt tattacatgt acaacatact gcttcaaata
     1261 cattcccatg taatgatata acttttgtat tcccactagc ttcatgtaat ccatctacat
     1321 tttgtgttac tactgatttt aaataaccca aactttctaa agttgataaa gctacatgac
     1381 cattgtttat ttctatttca taatcggatg atatatctct tattacttcc catatctttt
     1441 caggatactt ccaaaaaccc catatagttc catatattct tgggtcatac ttactccata
     1501 tcgaattcga tgaccctcga aaacttggga tgttactttc tgcagatgta cctgaccctg
     1561 ttaaggcaac tacatgtttg cattttttta ttattttggc tagctcctcc aaagtaatac
     1621 tttgtgtgtc ttttttcaaa aaggaaatca ttaaattacc catgctgccg ctgccagagc
     1681 cctttttctt ttttgcctgg ccggcctttt tcgtggccgc cggccttttg tcgcctccca
     1741 gctgagacag gtcgatccgt gtctcgtaca ggccggtgat gctctggtgg atcagggtgg
     1801 cgtccagcac ctctttggtg ctggtgtacc tcttccggtc gatggtggtg tcaaagtact
     1861 tgaaggcggc aggggctccc agattggtca gggtaaacag gtggatgata ttctcggcct
     1921 gctctctgat gggcttatcc cggtgcttgt tgtaggcgga cagcactttg tccagattag
     1981 cgtcggccag gatcactctc ttggagaact cgctgatctg ctcgatgatc tcgtccaggt
     2041 agtgcttgtg ctgttccaca aacagctgtt tctgctcatt atcctcgggg gagcccttca
     2101 gcttctcata gtggctggcc aggtacagga agttcacata tttggagggc agggccagtt
     2161 cgtttccctt ctgcagttcg ccggcagagg ccagcattct cttccggccg ttttccagct
     2221 cgaacaggga gtacttaggc agcttgatga tcaggtcctt tttcacttct ttgtagccct
     2281 tggcttccag aaagtcgatg ggattcttct cgaagctgct tctttccatg atggtgatcc
     2341 ccagcagctc tttcacactc ttcagtttct tggacttgcc cttttccact ttggccacca
     2401 ccagcacaga ataggccacg gtggggctgt cgaagccgcc gtacttctta gggtcccagt
     2461 ccttctttct ggcgatcagc ttatcgctgt tcctcttggg caggatagac tctttgctga
     2521 agccgcctgt ctgcacctcg gtctttttca cgatattcac ttggggcatg ctcagcactt
     2581 tccgcacggt ggcaaaatcc cggcccttat cccacacgat ctccccggtt tcgccgtttg
     2641 tctcgatcag aggccgcttc cggatctcgc cgttggccag ggtaatctcg gtcttgaaaa
     2701 agttcatgat gttgctgtag aagaagtact tggcggtagc cttgccgatt tcctgctcgc
     2761 tcttggcgat catcttccgc acgtcgtaca ccttgtagtc gccgtacacg aactcgcttt
     2821 ccagcttagg gtactttttg atcagggcgg ttcccacgac ggcgttcagg taggcgtcgt
     2881 gggcgtggtg gtagttgttg atctcgcgca ctttgtaaaa ctggaaatcc ttccggaaat
     2941 cggacaccag cttggacttc agggtgatca ctttcacttc ccggatcagc ttgtcattct
     3001 cgtcgtactt agtgttcatc cgggagtcca ggatctgtgc cacgtgcttt gtgatctgcc
     3061 gggtttccac cagctgtctc ttgatgaagc cggccttatc cagttcgctc aggccgcctc
     3121 tctcggcctt ggtcagattg tcgaactttc tctgggtaat cagcttggcg ttcagcagct
     3181 gccgccagta gttcttcatc ttcttcacga cctcttcgga gggcacgttg tcgctcttgc
     3241 cccggttctt gtcgcttctg gtcagcacct tgttgtcgat ggagtcgtcc ttcagaaagc
     3301 tctgaggcac gattgcgtcc acatcgtagt cggacagccg gttgatgtcc agttcctggt
     3361 ccacgtacat atcccgccca ttctgcaggt agtacaggta cagcttctcg ttctgcagct
     3421 gggtgttttc cacggggtgt tctttcagga tctggctgcc cagctctttg atgccctctt
     3481 cgatccgctt cattctctcg cggctgttct tctgtccctt ctgggtggtc tggttctctc
     3541 tggccatttc gatcacgatg ttctcgggct tgtgccggcc catcactttc acgagctcgt
     3601 ccaccacctt cactgtctgc aggatgccct tcttaatggc ggggctgccg gccagattgg
     3661 caatgtgctc gtgcaggcta tcgccctggc cggacacctg ggctttctgg atgtcctctt
     3721 taaaggtcag gctgtcgtcg tggatcagct gcatgaagtt tctgttggcg aagccgtcgg
     3781 acttcaggaa atccaggatt gtcttgccgg actgcttgtc ccggatgccg ttgatcagct
     3841 tccggctcag cctgccccag ccggtgtatc tccgccgctt cagctgcttc atcactttgt
     3901 cgtcgaacag gtgggcatag gttttcagcc gttcctcgat catctctctg tcctcaaaca
     3961 gtgtcagggt cagcacgata tcttccagaa tgtcctcgtt ttcctcattg tccaggaagt
     4021 ccttgtcctt gataattttc agcagatcgt ggtatgtgcc cagggaggcg ttgaaccgat
     4081 cttccacgcc ggagatttcc acggagtcga agcactcgat tttcttgaag tagtcctctt
     4141 tcagctgctt cacggtcact ttccggttgg tcttgaacag caggtccacg atggcctttt
     4201 tctgctcgcc gctcaggaag gcgggctttc tcattccctc ggtcacgtat ttcactttgg
     4261 tcagctcgtt atacacggtg aagtactcgt acagcaggct gtgcttgggc agcaccttct
     4321 cgttgggcag gttcttatcg aagttggtca tccgctcgat gaagctctgg gcggaagcgc
     4381 ccttgtccac cacttcctcg aagttccagg gggtgatggt ttcctcgctc tttctggtca
     4441 tccaggcgaa tctgctgttt cccctggcca gagggcccac gtagtagggg atgcggaagg
     4501 tcaggatctt ctcgatcttt tcccggttgt ccttcaggaa tgggtaaaaa tcttcctgcc
     4561 gccgcagaat ggcgtgcagc tctcccaggt ggatctggtg ggggatgctg ccgttgtcga
     4621 aggtccgctg cttccgcagc aggtcctctc tgttcagctt cacgagcagt tcctcggtgc
     4681 cgtccatctt ttccaggatg ggcttgatga acttgtagaa ctcttcctgg ctggctccgc
     4741 cgtcaatgta gccggcgtag ccgttcttgc tctggtcgaa gaaaatctct ttgtacttct
     4801 caggcagctg ctgccgcacg agagctttca gcagggtcag gtcctggtgg tgctcgtcgt
     4861 atctcttgat catagaggcg ctcagggggg ccttggtgat ctcggtgttc actctcagga
     4921 tgtcgctcag caggatggcg tcggacaggt tcttggcggc cagaaacagg tcggcgtact
     4981 ggtcgccgat ctgggccagc aggttgtcca ggtcgtcgtc gtaggtgtcc ttgctcagct
     5041 gcagtttggc atcctcggcc aggtcgaagt tgctcttgaa gttgggggtc aggcccaggc
     5101 tcagggcaat caggtttccg aacaggccat tcttcttctc gccgggcagc tgggcgatca
     5161 gattttccag ccgtctgctc ttgctcagtc tggcagacag gatggccttg gcgtccacgc
     5221 cgctggcgtt gatggggttt tcctcgaaca gctggttgta ggtctgcacc agctggatga
     5281 acagcttgtc cacgtcgctg ttgtcggggt tcaggtcgcc ctcgatcagg aagtggcccc
     5341 ggaacttgat catgtgggcc agggccagat agatcagccg caggtcggcc ttgtcggtgc
     5401 tgtccaccag tttctttctc aggtggtaga tggtggggta cttctcgtgg taggccacct
     5461 cgtccacgat gttgccgaag atggggtgcc gctcgtgctt cttatcctct tccaccagga
     5521 aggactcttc cagtctgtgg aagaagctgt cgtccacctt ggccatctcg ttgctgaaga
     5581 tctcttgcag atagcagatc cggttcttcc gtctggtgta tcttcttctg gcggttctct
     5641 tcagccgggt ggcctcggct gtttcgccgc tgtcgaacag cagggctccg atcaggttct
     5701 tcttgatgct gtgccggtcg gtgttgccca gcaccttgaa tttcttgctg ggcaccttgt
     5761 actcgtcggt gatcacggcc cagcccacag agttggtgcc gattgccagg ccgatgctgt
     5821 acttcttgtc ggctgctggg actccgtgga taccgacctt ccgcttcttc tttggggcca
     5881 tcttatcgtc atcgtctttg taatcaatat catgatcctt gtagtctccg tcgtggtcct
     5941 tatagtccat ctcgagttta ttcgaaatgt gggaagaaaa aaaatataat aaaaaaggaa
     6001 aatttctttt taaagtaaaa tatttatgta aatatttata tattttatta tatatatatt
     6061 attatatata tatatatata tatatatata ccaataaaat aatataaaaa tttttatgta
     6121 tatctaaaaa tatgttttta ttaaaaggta aaaataaact tattttttct ttttttttaa
     6181 aaaatatata tatatatata tatatataat attatatgag atatatatta aatattatat
     6241 tatttatata aatataattt ttttcttaat ttctcacgtt gttaaaattt atatatttat
     6301 atatgatata tattttttaa tactttttaa ttttttttat tatttttggt aattatctta
     6361 ttttatttta attttattat tttatattaa atgatgcttt ttattttata ttattatttt
     6421 ctatttaaaa tttttgctaa acatgaaatt attttattat attttgtaaa tacaaaataa
     6481 aataatatat aaatcaaaaa aaaaaaagaa agaaagaaag aaagatgata tttaaaaaaa
     6541 aaaagatatt ataaaaatat aatattatat ataaaatatt atttttatat aaatatattt
     6601 atatatttat atatatgtat atatatatat atatatatat aggtatagta tttattattt
     6661 tgttatattt atataatact cctttttgtt attttttttt ttttttcttt aaattcatgc
     6721 aaaaatttac tataatatta ttatatttaa ctatatacta tggaatacta aatatatatc
     6781 caatggcccc tttccgcggg gaggactagt acgaagatct gtcttaagct tagctaattc
     6841 gcttgtaaga ggtactctcg tttatgcaaa actatttgat atagcatttt aacaagtaca
     6901 catatatata tgtaatatat atactatata tatctattgc atgtgtacta agcatgtgca
     6961 tggcatcccc tttttctcgt gtttaaaaca gtttgtatga taaaatataa aggatttgaa
     7021 aaagagaaaa aaatatatga tctcatccta tatagcgcca taatttttat ttgggttgaa
     7081 taaaattttc tactaaattt aggtgtaagt aaaataatgg aatatatata agtacaataa
     7141 aaaagtgcat aaattaaaaa atttttataa taaatatttt ttttaaaaaa gtcaataata
     7201 atattaaata tatataacac aggattatat atgttcacta caatttttta tattataata
     7261 taaattcttt tcaattttca ttttatttta catacacttt ccttttttgt cactatattt
     7321 taatattcac atatttagtt taaatactgg ctatttcttt ctacatttgc tagtaacaat
     7381 tgtgtagtgc ttatatatat acacacacct aaaacttaca aaccatggtg ataaatgcat
     7441 gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac
     7501 agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc
     7561 atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg
     7621 ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat
     7681 gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat
     7741 cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt
     7801 gaattgctgc cctctggtta tgtgtgggag ggctaaggta ccccattaaa tttatttaat
     7861 aatagattaa aaatattata aaaataaaaa cataaacaca gaaattacaa aaaaaataca
     7921 tatgaatttt tttttgtaat cttccttata aatatagaat aatgaatcat ataaaacata
     7981 tcattgttca tttatttaca tttaaaatta ttgtttcagt atctttaatt tattatgtat
     8041 atataaaaat aacttacaat tttattaata aacaatatat gtttattaat tcatgttttg
     8101 taatttatgg gatagcgatt ttttttactg tctgtatttt tcttttttaa ttatgtttta
     8161 attgtatttt atttttatta ttgttctttt tatagtatta ttttaaaaca aaatgcattt
     8221 tctaagaact tataataata ataaatataa attttaataa aaattatatt tatcttttac
     8281 aatatgaaca taaagtacaa cattaatata tagcttttaa tatttttatt cctaatcatg
     8341 taaatcttaa atttttcttt ttaaacatat gttaaatatt tatttctcat tatatataag
     8401 gacatattta ttaaaccgca gagaaatcta gaggtacgaa ttcatatcga taactccatg
     8461 gaactcctag gctggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     8521 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     8581 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     8641 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     8701 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     8761 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     8821 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     8881 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     8941 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     9001 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     9061 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     9121 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     9181 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     9241 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     9301 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     9361 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     9421 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     9481 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     9541 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     9601 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     9661 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     9721 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     9781 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     9841 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     9901 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     9961 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    10021 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    10081 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    10141 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    10201 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    10261 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    10321 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    10381 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    10441 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    10501 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    10561 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    10621 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    10681 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
    10741 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
    10801 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
    10861 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
    10921 ggaaacagct atgaccatga ttacgccaag ctatttaggt gacactatag aa
//