pGL3-G/S/A-Nicking/PegRNA-PGK-puroR-mRFP1

价格：元

规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg

相关技术服务：质粒构建    基因合成    质粒大提

联系方式：I47-825O-882O

买家导航

pGL3-G/S/A-Nicking/PegRNA-PGK-puroR-mRFP1载体质粒基本信息

质粒名称: pGL3-G/S/A-Nicking/PegRNA-PGK-puroR-mRFP1
出品公司: Wekwikgene
目录编号: 0002189
产品用途: hCtRNA simultaneously drives the expression of NickingRNA and PegRNA targeting three different genes: GATA2, SETBP1, ASXL1, harboring dual selection markers of puroR and RFP.
存储实验室: Zhuobin Liang Lab 梁卓斌课题组

载体骨架基本信息
载体名称: pGL3
表达系统: 哺乳动物表达
启动子: hCtRNA
拷贝数: 高拷贝
载体抗性: 氨苄霉素
生长温度: 37 ℃
宿主菌: DH5alpha

插入基因信息
插入基因名称: GATA2-pegRNA-nicking-sgRNA, SETBP1-pegRNA-nicking-sgRNA, ASXL1-pegRNA-nicking-sgRNA
物种来源: H.sapiens
密码子优化: 未优化

文献引用方法
参考文献部分:
TRU-PE: A Universal, Trackable Prime Editor Toolkit for Robust Single-and Multi-Locus Genome Engineering
Zhichao Qiu, Keke Sun, Qingwei Zeng, Ziwei Luo, Xinran Liu, Yaping Li, Xiang Lei, Ruilin Zhao, Zhen Zhang, Damia Romero-Moya, Yuan Yang, Xuan Wang, Ehsan Hashemi, Joe Z. Zhang, Xiaolong Wang, Alessandra Giorgetti, Zhuobin Liang
bioRxiv 2026.02.20.706938

pGL3-G/S/A-Nicking/PegRNA-PGK-puroR-mRFP1质粒图谱载体图谱和pGL3-G/S/A-Nicking/PegRNA-PGK-puroR-mRFP1载体序列质粒序列多克隆位点信息

pGL3载体图谱

pGL3-G/S/A-Nicking/PegRNA-PGK-puroR-mRFP1质粒载体简介

pGL3-G/S/A-Nicking/PegRNA-PGK-puroR-mRFP1质粒序列载体序列

LOCUS       pGL3-G/S/A-Nicking/PegRNA-PGK-puroR-mRFP1              6487 bp DNA     circular SYN 06-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 20, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..61
                     /label=OL1451
     misc_feature    41..115
                     /label=hCtRNA
     primer_bind     complement(98..144)
                     /label=OL2595
     primer_bind     complement(98..117)
                     /label=OL2608
     primer_bind     complement(98..116)
                     /label=OL2596
     primer_bind     complement(98..115)
                     /label=OL2600
     primer_bind     complement(98..115)
                     /label=OL2602
     primer_bind     complement(98..115)
                     /label=OL2606
     misc_feature    116..135
                     /label=GATA2 nickingRNA
     primer_bind     135..171
                     /label=OL2603
     primer_bind     complement(136..215)
                     /label=OL476
     misc_RNA        136..211
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     136..207
                     /label=OL2116
     primer_bind     136..171
                     /label=OL2597
     primer_bind     136..171
                     /label=OL2601
     primer_bind     136..171
                     /label=OL2607
     primer_bind     136..171
                     /label=OL2609
     primer_bind     complement(140..211)
                     /label=OL2117
     primer_bind     140..177
                     /label=OL1453
     primer_bind     complement(183..202)
                     /label=OL1927
     primer_bind     complement(188..207)
                     /label=OL1940
     primer_bind     complement(194..212)
                     /label=OL2610
     primer_bind     complement(194..211)
                     /label=OL2598
     primer_bind     complement(194..211)
                     /label=OL2604
     primer_bind     complement(194..211)
                     /label=OL769
     primer_bind     208..286
                     /label=OL477
     misc_feature    212..286
                     /label=hCtRNA
     primer_bind     212..232
                     /label=OL1451
     primer_bind     complement(269..298)
                     /label=OL2596
     primer_bind     complement(269..287)
                     /label=OL2595
     primer_bind     complement(269..287)
                     /label=OL2608
     primer_bind     complement(269..286)
                     /label=OL2600
     primer_bind     complement(269..286)
                     /label=OL2602
     primer_bind     complement(269..286)
                     /label=OL2606
     misc_feature    287..306
                     /label=GATA2 spacer
     primer_bind     295..342
                     /label=OL2597
     misc_RNA        307..382
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     307..378
                     /label=OL2116
     primer_bind     307..342
                     /label=OL2601
     primer_bind     307..342
                     /label=OL2603
     primer_bind     307..342
                     /label=OL2607
     primer_bind     307..342
                     /label=OL2609
     primer_bind     complement(311..382)
                     /label=OL2117
     primer_bind     311..348
                     /label=OL1453
     primer_bind     complement(354..373)
                     /label=OL1927
     primer_bind     complement(359..378)
                     /label=OL1940
     primer_bind     complement(365..422)
                     /label=OL2610
     primer_bind     complement(365..401)
                     /label=OL2598
     primer_bind     complement(365..382)
                     /label=OL2604
     primer_bind     complement(365..382)
                     /label=OL769
     misc_feature    383..394
                     /label=RTT
     misc_feature    395..410
                     /label=PBS
     primer_bind     398..437
                     /label=OL2599
     primer_bind     411..437
                     /label=OL2605
     primer_bind     447..471
                     /label=OL1451
     misc_feature    451..525
                     /label=hCtRNA
     primer_bind     complement(508..541)
                     /label=OL2600
     primer_bind     complement(508..525)
                     /label=OL2595
     primer_bind     complement(508..525)
                     /label=OL2596
     primer_bind     complement(508..525)
                     /label=OL2602
     primer_bind     complement(508..525)
                     /label=OL2606
     primer_bind     complement(508..525)
                     /label=OL2608
     misc_feature    526..545
                     /label=SETBP1 nickingRNA
     primer_bind     529..581
                     /label=OL2601
     primer_bind     543..581
                     /label=OL2607
     primer_bind     545..581
                     /label=OL2609
     primer_bind     complement(546..625)
                     /label=OL476
     misc_RNA        546..621
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     546..617
                     /label=OL2116
     primer_bind     546..581
                     /label=OL2597
     primer_bind     546..581
                     /label=OL2603
     primer_bind     complement(550..621)
                     /label=OL2117
     primer_bind     550..587
                     /label=OL1453
     primer_bind     complement(593..612)
                     /label=OL1927
     primer_bind     complement(598..617)
                     /label=OL1940
     primer_bind     complement(604..622)
                     /label=OL2610
     primer_bind     complement(604..621)
                     /label=OL2598
     primer_bind     complement(604..621)
                     /label=OL2604
     primer_bind     complement(604..621)
                     /label=OL769
     primer_bind     618..696
                     /label=OL477
     misc_feature    622..696
                     /label=hCtRNA
     primer_bind     622..642
                     /label=OL1451
     primer_bind     complement(679..725)
                     /label=OL2595
     primer_bind     complement(679..710)
                     /label=OL2602
     primer_bind     complement(679..696)
                     /label=OL2596
     primer_bind     complement(679..696)
                     /label=OL2600
     primer_bind     complement(679..696)
                     /label=OL2606
     primer_bind     complement(679..696)
                     /label=OL2608
     misc_feature    697..716
                     /label=SETBP1 spacer
     primer_bind     707..752
                     /label=OL2603
     misc_RNA        717..792
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     717..788
                     /label=OL2116
     primer_bind     717..752
                     /label=OL2597
     primer_bind     717..752
                     /label=OL2601
     primer_bind     717..752
                     /label=OL2607
     primer_bind     717..752
                     /label=OL2609
     primer_bind     complement(721..792)
                     /label=OL2117
     primer_bind     721..758
                     /label=OL1453
     primer_bind     complement(764..783)
                     /label=OL1927
     primer_bind     complement(769..788)
                     /label=OL1940
     primer_bind     complement(775..828)
                     /label=OL2610
     primer_bind     complement(775..807)
                     /label=OL2604
     primer_bind     complement(775..792)
                     /label=OL2598
     primer_bind     complement(775..792)
                     /label=OL769
     misc_feature    793..803
                     /label=RTT
     primer_bind     804..843
                     /label=OL2605
     misc_feature    804..816
                     /label=PBS
     primer_bind     817..843
                     /label=OL2599
     primer_bind     853..877
                     /label=OL1451
     misc_feature    857..931
                     /label=hCtRNA
     primer_bind     complement(914..948)
                     /label=OL2606
     primer_bind     complement(914..931)
                     /label=OL2595
     primer_bind     complement(914..931)
                     /label=OL2596
     primer_bind     complement(914..931)
                     /label=OL2600
     primer_bind     complement(914..931)
                     /label=OL2602
     primer_bind     complement(914..931)
                     /label=OL2608
     misc_feature    932..951
                     /label=ASXL1 nickingRNA
     primer_bind     942..987
                     /label=OL2607
     primer_bind     949..987
                     /label=OL2601
     primer_bind     951..987
                     /label=OL2609
     primer_bind     complement(952..1031)
                     /label=OL476
     misc_RNA        952..1027
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     952..1023
                     /label=OL2116
     primer_bind     952..987
                     /label=OL2597
     primer_bind     952..987
                     /label=OL2603
     primer_bind     complement(956..1027)
                     /label=OL2117
     primer_bind     956..993
                     /label=OL1453
     primer_bind     complement(999..1018)
                     /label=OL1927
     primer_bind     complement(1004..1023)
                     /label=OL1940
     primer_bind     complement(1010..1028)
                     /label=OL2610
     primer_bind     complement(1010..1027)
                     /label=OL2598
     primer_bind     complement(1010..1027)
                     /label=OL2604
     primer_bind     complement(1010..1027)
                     /label=OL769
     primer_bind     1024..1102
                     /label=OL477
     misc_feature    1028..1102
                     /label=hCtRNA
     primer_bind     1028..1048
                     /label=OL1451
     primer_bind     complement(1085..1118)
                     /label=OL2608
     primer_bind     complement(1085..1104)
                     /label=OL2595
     primer_bind     complement(1085..1103)
                     /label=OL2596
     primer_bind     complement(1085..1102)
                     /label=OL2600
     primer_bind     complement(1085..1102)
                     /label=OL2602
     primer_bind     complement(1085..1102)
                     /label=OL2606
     misc_feature    1103..1122
                     /label=ASXL1 spacer
     primer_bind     1113..1158
                     /label=OL2609
     primer_bind     1122..1158
                     /label=OL2601
     primer_bind     1122..1158
                     /label=OL2607
     misc_RNA        1123..1198
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     1123..1194
                     /label=OL2116
     primer_bind     1123..1158
                     /label=OL2597
     primer_bind     1123..1158
                     /label=OL2603
     primer_bind     complement(1127..1198)
                     /label=OL2117
     primer_bind     1127..1164
                     /label=OL1453
     primer_bind     complement(1170..1189)
                     /label=OL1927
     primer_bind     complement(1175..1194)
                     /label=OL1940
     primer_bind     complement(1181..1232)
                     /label=OL2610
     primer_bind     complement(1181..1198)
                     /label=OL2598
     primer_bind     complement(1181..1198)
                     /label=OL2604
     primer_bind     complement(1181..1198)
                     /label=OL769
     misc_feature    1199..1212
                     /label=RTT
     misc_feature    1213..1220
                     /label=PBS
     primer_bind     complement(1233..1252)
                     /label=OL1916
     primer_bind     complement(1247..1263)
                     /label=OL1168
     misc_feature    1264..1381
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(1318..1337)
                     /label=OL1936
     primer_bind     complement(1324..1348)
                     /label=OL1571
     primer_bind     complement(1400..1419)
                     /label=OL1088
     promoter        1430..1940
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1524..1543)
                     /label=OL1068
     primer_bind     1672..1694
                     /label=OL1709
     primer_bind     1714..1730
                     /label=OL446
     primer_bind     1859..1877
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(1942..1961)
                     /label=OL2256
     primer_bind     complement(1943..1964)
                     /label=OL1702
     CDS             1962..2558
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1962..1981)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1965..1984
                     /label=OL2264
     primer_bind     2458..2478
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2458..2477
                     /label=OL2294
     primer_bind     2458..2476
                     /label=OL2339
     primer_bind     2529..2583
                     /label=OL901
     primer_bind     complement(2535..2558)
                     /label=OL306
     primer_bind     complement(2544..2566)
                     /label=OL384
     primer_bind     2551..2582
                     /label=OL379
     CDS             2559..2615
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     2559..2615
                     /label=OL866
     primer_bind     complement(2559..2615)
                     /label=OL867
     primer_bind     2559..2585
                     /label=OL2251
     primer_bind     2596..2639
                     /label=OL902
     primer_bind     complement(2596..2623)
                     /label=OL966
     RBS             2598..2605
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2616..3293
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     2616..2634
                     /label=OL2300
     primer_bind     2847..2866
                     /label=OL1123
     primer_bind     complement(2856..2882)
                     /label=OL1728
     primer_bind     complement(2888..2914)
                     /label=OL2455
     primer_bind     complement(3271..3318)
                     /label=OL903
     primer_bind     complement(3271..3293)
                     /label=OL2353
     primer_bind     complement(3272..3293)
                     /label=OL2708
     primer_bind     3292..3310
                     /label=OL965
     primer_bind     3294..3316
                     /label=OL305
     polyA_signal    3440..3561
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     3440..3476
                     /label=OL1698
     primer_bind     complement(3451..3470)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3505..3524
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3545..3577)
                     /label=OL2209
     primer_bind     3562..3595
                     /label=OL1692
     primer_bind     complement(3575..3601)
                     /label=OL1941
     primer_bind     complement(3750..3773)
                     /label=OL1043
     primer_bind     3751..3773
                     /label=OL1044
     primer_bind     complement(3809..3826)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3980..4568)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4060..4079)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(4162..4181)
                     /label=OL1041
     primer_bind     complement(4295..4314)
                     /label=OL631
     primer_bind     4351..4370
                     /label=OL798
     primer_bind     complement(4592..4631)
                     /label=OL660
     primer_bind     4612..4631
                     /label=OL659
     CDS             complement(4739..5599)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5196..5215
                     /label=OL1922
     primer_bind     5362..5381
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(5362..5381)
                     /label=OL1923
     primer_bind     5515..5534
                     /label=OL1033
     primer_bind     complement(5515..5534)
                     /label=OL1034
     promoter        complement(5600..5704)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5731..6186
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5756..5765)
                     /label=OL305
     primer_bind     complement(5818..5837)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6028..6049
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6239..6258
                     /label=OL1469
     primer_bind     6248..6264
                     /label=OL1109
     polyA_signal    6317..6365
                     /note="synthetic polyadenylation signal"
     misc_feature    6379..6470
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     6379..6395
                     /label=OL1167
     primer_bind     6425..6444
                     /label=OL1087
     primer_bind     6449..6463
                     /label=OL1935
     primer_bind     join(6479..6487,1..17)
                     /label=OL1700
ORIGIN
        1 agtgaacgga tctcgacggt atcgatcacg agactagcct agagggggta tagctcagtg
       61 gtagagcatt tgactgcaga tcaagaggtc cccggttcaa atccgggtgc cccctacatc
      121 ttccggttct gagtcgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
      181 tcaacttgaa aaagtggcac cgagtcggtg cagagggggt atagctcagt ggtagagcat
      241 ttgactgcag atcaagaggt ccccggttca aatccgggtg ccccctagaa ggaagggatc
      301 cagactgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
      361 aaaagtggca ccgagtcggt gctcttccgg ttctgagtct ggatcccttc tttttttctc
      421 gacctcgaga caagatcacg agactagcct agagggggta tagctcagtg gtagagcatt
      481 tgactgcaga tcaagaggtc cccggttcaa atccgggtgc cccctgagac gatccccagc
      541 aacaggtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
      601 aaagtggcac cgagtcggtg cagagggggt atagctcagt ggtagagcat ttgactgcag
      661 atcaagaggt ccccggttca aatccgggtg cccccttgtc ccaatgccgc tgtcgcgttt
      721 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
      781 ccgagtcggt gcgatcccca gcaacagcgg cattggtttt tttctcgacc tcgagacaag
      841 atcacgagac tagcctagag ggggtatagc tcagtggtag agcatttgac tgcagatcaa
      901 gaggtccccg gttcaaatcc gggtgccccc tccccccccc tccgatggca ggttttagag
      961 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     1021 tcggtgcaga gggggtatag ctcagtggta gagcatttga ctgcagatca agaggtcccc
     1081 ggttcaaatc cgggtgcccc ctactgccat cggagggggg gggttttaga gctagaaata
     1141 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgcac
     1201 ccgggccacc ccccccctcc tttttttctc gacctcgaga caaatggcag tattcatcca
     1261 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1321 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1381 tcgggtttat tacagggaca gcagagatcc actttggccg cggctcgagg gggttggggt
     1441 tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt
     1501 ccgggaaacg cagcggcgcc gaccctggga ctcgcacatt cttcacgtcc gttcgcagcg
     1561 tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc
     1621 taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac
     1681 gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc
     1741 gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag
     1801 gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt
     1861 gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa
     1921 tcaccgacct ctctccccag ggggatccac cggagcttac catgaccgag tacaagccca
     1981 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     2041 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     2101 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     2161 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     2221 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     2281 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     2341 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     2401 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggaaacctcc gcgccccgca
     2461 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     2521 gaccgcgcac ctggtgcatg acccgcaagc ccggtgccgc tactaacttc agcctgctga
     2581 agcaggctgg agacgtggag gagaaccctg gacctatggc gagtagcgaa gacgttatca
     2641 aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa
     2701 tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta
     2761 ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt
     2821 ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg
     2881 aaggtttcaa atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc
     2941 aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact
     3001 tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac
     3061 gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag
     3121 acggtggtca ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc
     3181 tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca
     3241 ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taacgcccgc
     3301 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg gtacctttaa
     3361 gaccaatgac ttacaaggca gctgtagatc ttagccactt tctagagtcg gggcggccgg
     3421 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     3481 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     3541 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     3601 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat
     3661 aaggatccgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     3721 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     3781 acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     3841 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3901 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3961 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4021 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4081 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4141 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4201 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4261 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4321 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4381 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     4441 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4501 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     4561 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     4621 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     4681 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     4741 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     4801 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     4861 gtgctgcaat gataccgcgg gacccacgct caccggctcc agatttatca gcaataaacc
     4921 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     4981 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     5041 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     5101 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     5161 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     5221 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     5281 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     5341 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     5401 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     5461 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     5521 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     5581 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     5641 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     5701 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
     5761 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5821 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5881 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5941 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6001 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6061 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6121 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     6181 caatttgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     6241 cttcgctatt acgccagccc aagctaccat gataagtaag taatattaag gtacgggagg
     6301 tacttggagc ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt
     6361 gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact
     6421 agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct atcgataggt
     6481 accgatt
//

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