pAAVLINK-EF1a-HA-5'-CHD8

LOCUS       pAAVLINK-EF1a-HA-5'-CHD8                7361 bp DNA     circular SYN 20-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7361)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 3, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        158..700
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    505..510
                     /label=SDS
     CDS             716..742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             749..4501
                     /codon_start=1
                     /label=CHD8
                     /translation="ADPIMDLFDDPNLFGLDSLTDDSFNQVTQDPIEEALGLPSSLDSL
                     DQMNQDGGGGDVGNSSASELVPPPEETAPTELSKESTAPAPESITLHDYTTQPASQEQP
                     AQPVLQTSTPTSGLLQVSKSQEILSQGNPFMGVSATAVSSSSAGGQPPQSAPKIVILKA
                     PPSSSVTGAHVAQIQAQGITSTAQPLVAGTANGGKVTFTKVLTGTPLRPGVSIVSGNTV
                     LAAKVPGNQAAVQRIVQPSRPVKQLVLQPVKGSAPAGNPGATGPPLKPAVTLTSTPTQG
                     ESKRITLVLQQPQSGGPQGHRHVVLGSLPGKILQGNQLAALTQAKNAQGQPAKVVTIQL
                     QVQQPQQKIQIVPQPPSSQPQPQQPPSTQPVTLSSVQQAQIMGPGQSPGQRLSVPVKVV
                     LQPQAGSSQGASSGLSVVKVLSASEVAALSSPASSAPHSGGKTGMEENRRLEHQKKQEK
                     ANRIVAEAIARARARGEQNIPRVLNEDELPSVRPEEEGEKKRRKKSAGERLKEEKPKKS
                     KTSGASKTKGKSKLNTITPVVGKKRKRNTSSDNSDVEVMPAQSPREDEESSIQKRRSNR
                     QVKRKKYTEDLDIKITDDEEEEEVDVTGPIKPEPILPEPVQEPDGETLPSMQFFVENPS
                     EEDAAIVDKVLSMRIVKKELPSGQYTEAEEFFVKYKNYSYLHCEWATISQLEKDKRIHQ
                     KLKRFKTKMAQMRHFFHEDEEPFNPDYVEVDRILDESHSIDKDNGEPVIYYLVKWCSLP
                     YEDSTWELKEDVDEGKIREFKRIQSRHPELKRVNRPQASAWKKLELSHEYKNRNQLREY
                     QLEGVNWLLFNWYNRQNCILADEMGLGKTIQSIAFLQEVYNVGIHGPFLVIAPLSTITN
                     WEREFNTWTEMNTIVYHGSLASRQMIQQYEMYCKDSRGRLIPGAYKFDALITTFEMILS
                     DCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLL
                     HFLEPSQFPSESEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIE
                     VELTNIQKKYYRAILEKNFSFLSKGAGHTNMPNLLNTMMELRKCCNHPYLINGAEEKIL
                     TEFREACHIIPHDFHLQAMVRSAGKLVLIDKLLPKLKAGGHKVLIFSQMVRCLDILEDY
                     LIQRRYLYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCIIF
                     DSDWNPQNDLQAQARCHRIGQSKAVK"
     misc_feature    2226..2231
                     /label=SDS
     misc_feature    4256..4261
                     /label=SDS
     intron          4502..4542
                     /label=Splicing Donor
     misc_feature    4546..4579
                     /label=lox2272-JT1571
     repeat_region   4624..4764
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4624..4753
                     /label=AAV2 ITR
     rep_origin      4839..5294
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5576..5680
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5681..6541
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6712..7300
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
      721 ttatgacgta cctgactatg ctggatctgc agaccccatc atggatctgt tcgatgaccc
      781 aaatttattt ggcctggact ctctgactga tgacagcttt aaccaggtca cacaagaccc
      841 cattgaggaa gcccttggac tgccaagctc tctggactcc ttggatcaga tgaaccagga
      901 tggtggaggt ggtgatgtgg ggaattcatc agcaagtgaa ctggtccctc caccagagga
      961 aacagctccc acagaacttt ccaaagaatc cacagctcca gctccagaat ccataacctt
     1021 gcatgattat accactcagc ctgccagcca ggagcagcca gcccaacctg tcttacagac
     1081 atcgacgcca acatcaggac ttttgcaagt ctccaagagc caggagatcc tgagccaagg
     1141 gaatcctttc atgggtgtct ctgccacagc tgtctcctcc agtagtgctg gagggcagcc
     1201 acctcagtca gcccctaaga ttgttatcct taaggcccca ccaagctcct cagtcactgg
     1261 tgcccatgtg gcacaaattc aggcccaagg tatcaccagc acagctcagc ccctggtggc
     1321 aggcacagcc aatggtggaa aagtcacttt taccaaagtg ctaaccggca caccccttcg
     1381 accaggtgtt tccattgtct ctggtaatac agtgttggcc gccaaggtcc ctgggaacca
     1441 ggctgctgtt cagcgcattg tccagcccag ccgaccagta aagcagctgg tcctccagcc
     1501 agttaagggt tcagctcctg ctggaaaccc tggggccaca gggcccccac tgaagcctgc
     1561 agttacactg acctctacac ctacccaggg tgaatcgaaa cgcatcaccc tggtcctcca
     1621 gcagccacag tctggaggtc cccaaggaca tcggcatgtt gtgctaggga gtctaccagg
     1681 caagatatta cagggcaacc agctggcagc cctgactcaa gccaagaatg cccaagggca
     1741 gcctgccaag gtagtaacta tccagctgca ggtgcagcag ccacagcaaa aaatccagat
     1801 tgtaccacaa ccaccatcat cgcagccaca gccccagcag ccaccctcca cccagccagt
     1861 gactctgtcc tctgtacagc aggctcagat aatgggacca ggacaaagcc caggacaaag
     1921 actttcagta ccagtcaagg tggtactgca gccacaggct ggctcttccc aaggggcctc
     1981 ttctgggctc tctgtagtta aagttctgag tgccagtgaa gtggcagctt tgtcatcacc
     2041 agcaagctct gctcctcatt cggggggaaa gacaggaatg gaggaaaacc gcagattgga
     2101 acaccagaag aagcaagaga aagcaaatcg gattgtagca gaggccattg cgagagcccg
     2161 tgcccgcggt gagcagaaca tacctcgagt cttaaatgag gacgagttgc ccagcgttcg
     2221 gccagaggag gaaggcgaga agaaacgcag gaagaagagt gctggggaga ggctgaaaga
     2281 ggagaagcca aagaagagta aaacatctgg tgcctccaaa acaaagggca agagcaagct
     2341 caacaccatc actcctgtag tgggtaagaa gagaaaacgt aatacctcat ctgataattc
     2401 agatgtggaa gtcatgcctg cacagtcacc tcgagaagat gaagaaagca gcattcagaa
     2461 gagacgctca aaccgccaag ttaagcgaaa aaaatataca gaggacctgg atataaagat
     2521 cacagatgat gaagaagaag aagaggtgga tgtaactggt ccaataaaac ctgagcctat
     2581 cctccctgaa ccagtgcaag aaccagatgg cgagactttg ccttccatgc agttctttgt
     2641 ggagaatccc agtgaagaag atgcagccat tgtagacaaa gtgctttcta tgcggattgt
     2701 gaagaaggag ctcccttctg gacaatatac tgaagcagaa gaattctttg tcaagtacaa
     2761 gaactactcc tatctgcatt gtgaatgggc tactatctcc caactagaga aggataagag
     2821 gatacatcaa aaattaaagc gcttcaaaac caaaatggct cagatgagac acttcttcca
     2881 tgaggatgaa gagcccttta atccagacta cgtagaggtg gataggatat tggatgagtc
     2941 tcacagtatt gacaaggaca atggggagcc cgttatttac tacctggtga aatggtgctc
     3001 tctgccctat gaggatagca catgggagct aaaagaagat gttgatgagg gcaagattcg
     3061 agaatttaaa cggattcagt caaggcaccc agaactcaaa agggtgaatc gtccgcaggc
     3121 aagtgcctgg aagaaattgg agctatcaca tgaatataaa aacagaaacc agctacggga
     3181 atatcagttg gaaggggtta attggctgct ctttaattgg tataacaggc agaactgcat
     3241 cctggctgat gagatgggat tgggcaaaac tattcagtcc attgccttct tgcaggaagt
     3301 atataatgtg ggcatccatg gtcccttctt ggtcattgcc ccactgtcca caattactaa
     3361 ctgggagcga gaatttaata catggacaga aatgaacact attgtgtacc atggcagtct
     3421 ggccagcagg cagatgattc aacagtatga aatgtactgc aaagattcac ggggacgcct
     3481 catcccaggc gcatacaagt ttgacgctct gatcaccact tttgagatga ttttgtcaga
     3541 ttgtcctgag cttcgtgaaa ttgaatggcg ttgtgttatc attgatgaag cccatcgact
     3601 gaaaaaccgt aattgcaagc tgcttgatag tctcaagcac atggacctgg aacacaaggt
     3661 gctactcaca ggaacaccat tgcaaaatac tgtagaagaa ctgtttagct tgcttcattt
     3721 cttggaaccg tcacaatttc cctcagaatc agagtttctc aaggactttg gggatctcaa
     3781 gacagaggaa caggttcaaa agctacaggc cattcttaag ccaatgatgc tgagaagact
     3841 caaagaggat gttgaaaaaa acttggcacc caaacaggaa acaattattg aagtagagct
     3901 gactaatatc cagaagaaat actatcgggc tattttggag aagaatttct ccttcctttc
     3961 caaaggggca ggtcatacca acatgcctaa tctacttaac acaatgatgg agttgcgcaa
     4021 gtgctgcaac cacccatatc tcatcaatgg tgctgaagaa aaaatcctaa cagaattccg
     4081 tgaagcttgc catattatac ctcatgactt tcacctgcag gccatggttc gttcagccgg
     4141 caaactggtt cttattgaca agttgcttcc aaagcttaaa gctggtggcc ataaagttct
     4201 gatcttctct cagatggtgc gctgcctaga catcctagag gattatttaa tccagaggag
     4261 gtacttatat gaacgtattg atgggcgagt tagaggcaac cttcgacagg ctgccattga
     4321 ccgcttcagc aagcctgact cagaccgctt tgtcttctta ctgtgtaccc gggctggtgg
     4381 acttggtatt aatcttacag ctgctgatac ctgcatcatc tttgattcag actggaatcc
     4441 acaaaatgac ctgcaggccc aggcacgatg tcatcgaatt gggcagagca aagctgtgaa
     4501 ggtaagtatc aaggttacaa gacaggttta aggagaccaa taagctatta ttcgtatagg
     4561 atactttata cgaagttatt aagttatgga gaccggtgtg gcacgtgcgg accgagcggc
     4621 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     4681 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     4741 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     4801 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     4861 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc
     4921 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4981 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     5041 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     5101 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     5161 aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt
     5221 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     5281 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     5341 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     5401 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     5461 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     5521 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5581 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5641 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5701 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5761 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5821 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5881 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     5941 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     6001 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     6061 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     6121 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     6181 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     6241 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6301 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6361 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6421 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6481 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6541 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6601 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6661 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6721 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6781 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6841 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6901 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6961 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     7021 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     7081 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     7141 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7201 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7261 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7321 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//