| 质粒名称: | pCMV-cAICDA-BE3 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 113422 |
| 存储实验室: | Jia Chen |
|
载体骨架基本信息 |
|
| 载体名称: | CMV |
| 空载体大小: | 3399 bp |
| 完整质粒大小: | 8472bp |
| 载体类型: | 哺乳细胞表达、CRISPR |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | cAICDA-BE3 / pCMV-cAICDA-XTEN-D10A-SGGS-UGI-SGGS-NLS |
| 物种来源: | Ictalurus punctatus (channel catfish) |
| 插入大小: | 5073 bp |
| 启动子: | CMV |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | unknown (unknown if destroyed) |
| 5’测序引物 | CMV |
| 3’测序引物 | BGH poly reverse |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV-cAICDA-BE3 was a gift from Jia Chen (Addgene plasmid # 113422 ; http://n2t.net/addgene:113422 ; RRID:Addgene_113422) |
| 参考文献部分: | Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Wang X, Li J, Wang Y, Yang B, Wei J, Wu J, Wang R, Huang X, Chen J, Yang L. Nat Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198. 10.1038/nbt.4198 PubMed 30125268 |
买家导航
pCMV-cAICDA-BE3载体质粒基本信息
pCMV-cAICDA-BE3质粒图谱载体图谱和pCMV-cAICDA-BE3载体序列质粒序列多克隆位点信息
pCMV-cAICDA-BE3质粒载体简介
pCMV-cAICDA-BE3质粒序列载体序列
LOCUS pCMV-cAICDA-BE3 8472 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses cAICDA-BE3 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pCMV-cAICDA-BE3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8472)
AUTHORS Wang X, Li J, Wang Y, Yang V, Wei J, Wu J, Wang R, Huang X, Chen J,
Yang L
TITLE Efficient base editing in methylated regions with a human
APOBEC3A-Cas9 fusion.
JOURNAL Nat Biotechnol., 2018
REFERENCE 2 (bases 1 to 8472)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8472
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(10..29)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 201..580
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 581..784
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 735..755
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 826..845
/label=T7
/note="T7 promoter, forward primer"
promoter 826..844
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1533..5633
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5646..5894
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 5907..5927
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5936..5953
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(5976..5993)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5982..6206
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(6277..6293)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6277..6293)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6290..6312)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6301..6317
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6325..6355)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6370..6391
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6508..6525)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6679..7267)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6759..6778)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7438..8298)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8061..8080
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8299..8403)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
61 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
121 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
181 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
241 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
301 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
361 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
421 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
481 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
541 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
601 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
661 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
721 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
781 agctggttta gtgaaccgtc agatccgcta gagatccgcg gccgctaata cgactcacta
841 tagggagagc cgccaccatg agcaagctgg acagtgtgct gctgactcag aggaagttta
901 tttaccacta taagaatgtg cgctgggctc gtgggaggaa cgagacctac ctctgttttg
961 tggtcaagaa acgcaacagt cccgactcgc tctccttcga cttcggacac ctgcgcaatc
1021 gttctggctg ccatgtggag cttctcttcc tgagctatct tggggtactg tgcccaggtt
1081 tcttgggttc cggtgtggat ggtgtcaggg tggcttatgc catcacctgg ttctgttcct
1141 ggtcaccctg ttcaaactgt gcccatcgcc tttctcgctt catgtctcag atgcccaacc
1201 tgcggctgcg catcttcgtc tcgcgcctct acttctgtga cgaggaggac agtcaagaga
1261 gagagggact ccgttgcttg cagagggcag gtgtgcaagt gacagtcatg acctataaag
1321 attttttcta ctgttggcaa acctttgtgg ctcaaaatca gaaggctttc aaggcttggg
1381 acgaccttca ccagaactct atccgactgt ctcggaaact acagcgaatc ctgcagccta
1441 gtgagtctga agacctgagg gatggcttcg ctctgctggg ccttagcggc agcgagactc
1501 ccgggacctc agagtccgcc acacccgaaa gtgataaaaa gtattctatt ggtttagcca
1561 tcggcactaa ttccgttgga tgggctgtca taaccgatga atacaaagta ccttcaaaga
1621 aatttaaggt gttggggaac acagaccgtc attcgattaa aaagaatctt atcggtgccc
1681 tcctattcga tagtggcgaa acggcagagg cgactcgcct gaaacgaacc gctcggagaa
1741 ggtatacacg tcgcaagaac cgaatatgtt acttacaaga aatttttagc aatgagatgg
1801 ccaaagttga cgattctttc tttcaccgtt tggaagagtc cttccttgtc gaagaggaca
1861 agaaacatga acggcacccc atctttggaa acatagtaga tgaggtggca tatcatgaaa
1921 agtacccaac gatttatcac ctcagaaaaa agctagttga ctcaactgat aaagcggacc
1981 tgaggttaat ctacttggct cttgcccata tgataaagtt ccgtgggcac tttctcattg
2041 agggtgatct aaatccggac aactcggatg tcgacaaact gttcatccag ttagtacaaa
2101 cctataatca gttgtttgaa gagaacccta taaatgcaag tggcgtggat gcgaaggcta
2161 ttcttagcgc ccgcctctct aaatcccgac ggctagaaaa cctgatcgca caattacccg
2221 gagagaagaa aaatgggttg ttcggtaacc ttatagcgct ctcactaggc ctgacaccaa
2281 attttaagtc gaacttcgac ttagctgaag atgccaaatt gcagcttagt aaggacacgt
2341 acgatgacga tctcgacaat ctactggcac aaattggaga tcagtatgcg gacttatttt
2401 tggctgccaa aaaccttagc gatgcaatcc tcctatctga catactgaga gttaatactg
2461 agattaccaa ggcgccgtta tccgcttcaa tgatcaaaag gtacgatgaa catcaccaag
2521 acttgacact tctcaaggcc ctagtccgtc agcaactgcc tgagaaatat aaggaaatat
2581 tctttgatca gtcgaaaaac gggtacgcag gttatattga cggcggagcg agtcaagagg
2641 aattctacaa gtttatcaaa cccatattag agaagatgga tgggacggaa gagttgcttg
2701 taaaactcaa tcgcgaagat ctactgcgaa agcagcggac tttcgacaac ggtagcattc
2761 cacatcaaat ccacttaggc gaattgcatg ctatacttag aaggcaggag gatttttatc
2821 cgttcctcaa agacaatcgt gaaaagattg agaaaatcct aacctttcgc ataccttact
2881 atgtgggacc cctggcccga gggaactctc ggttcgcatg gatgacaaga aagtccgaag
2941 aaacgattac tccatggaat tttgaggaag ttgtcgataa aggtgcgtca gctcaatcgt
3001 tcatcgagag gatgaccaac tttgacaaga atttaccgaa cgaaaaagta ttgcctaagc
3061 acagtttact ttacgagtat ttcacagtgt acaatgaact cacgaaagtt aagtatgtca
3121 ctgagggcat gcgtaaaccc gcctttctaa gcggagaaca gaagaaagca atagtagatc
3181 tgttattcaa gaccaaccgc aaagtgacag ttaagcaatt gaaagaggac tactttaaga
3241 aaattgaatg cttcgattct gtcgagatct ccggggtaga agatcgattt aatgcgtcac
3301 ttggtacgta tcatgacctc ctaaagataa ttaaagataa ggacttcctg gataacgaag
3361 agaatgaaga tatcttagaa gatatagtgt tgactcttac cctctttgaa gatcgggaaa
3421 tgattgagga aagactaaaa acatacgctc acctgttcga cgataaggtt atgaaacagt
3481 taaagaggcg tcgctatacg ggctggggac gattgtcgcg gaaacttatc aacgggataa
3541 gagacaagca aagtggtaaa actattctcg attttctaaa gagcgacggc ttcgccaata
3601 ggaactttat gcagctgatc catgatgact ctttaacctt caaagaggat atacaaaagg
3661 cacaggtttc cggacaaggg gactcattgc acgaacatat tgcgaatctt gctggttcgc
3721 cagccatcaa aaagggcata ctccagacag tcaaagtagt ggatgagcta gttaaggtca
3781 tgggacgtca caaaccggaa aacattgtaa tcgagatggc acgcgaaaat caaacgactc
3841 agaaggggca aaaaaacagt cgagagcgga tgaagagaat agaagagggt attaaagaac
3901 tgggcagcca gatcttaaag gagcatcctg tggaaaatac ccaattgcag aacgagaaac
3961 tttacctcta ttacctacaa aatggaaggg acatgtatgt tgatcaggaa ctggacataa
4021 accgtttatc tgattacgac gtcgatcaca ttgtacccca atcctttttg aaggacgatt
4081 caatcgacaa taaagtgctt acacgctcgg ataagaaccg agggaaaagt gacaatgttc
4141 caagcgagga agtcgtaaag aaaatgaaga actattggcg gcagctccta aatgcgaaac
4201 tgataacgca aagaaagttc gataacttaa ctaaagctga gaggggtggc ttgtctgaac
4261 ttgacaaggc cggatttatt aaacgtcagc tcgtggaaac ccgccaaatc acaaagcatg
4321 ttgcacagat actagattcc cgaatgaata cgaaatacga cgagaacgat aagctgattc
4381 gggaagtcaa agtaatcact ttaaagtcaa aattggtgtc ggacttcaga aaggattttc
4441 aattctataa agttagggag ataaataact accaccatgc gcacgacgct tatcttaatg
4501 ccgtcgtagg gaccgcactc attaagaaat acccgaagct agaaagtgag tttgtgtatg
4561 gtgattacaa agtttatgac gtccgtaaga tgatcgcgaa aagcgaacag gagataggca
4621 aggctacagc caaatacttc ttttattcta acattatgaa tttctttaag acggaaatca
4681 ctctggcaaa cggagagata cgcaaacgac ctttaattga aaccaatggg gagacaggtg
4741 aaatcgtatg ggataagggc cgggacttcg cgacggtgag aaaagttttg tccatgcccc
4801 aagtcaacat agtaaagaaa actgaggtgc agaccggagg gttttcaaag gaatcgattc
4861 ttccaaaaag gaatagtgat aagctcatcg ctcgtaaaaa ggactgggac ccgaaaaagt
4921 acggtggctt cgatagccct acagttgcct attctgtcct agtagtggca aaagttgaga
4981 agggaaaatc caagaaactg aagtcagtca aagaattatt ggggataacg attatggagc
5041 gctcgtcttt tgaaaagaac cccatcgact tccttgaggc gaaaggttac aaggaagtaa
5101 aaaaggatct cataattaaa ctaccaaagt atagtctgtt tgagttagaa aatggccgaa
5161 aacggatgtt ggctagcgcc ggagagcttc aaaaggggaa cgaactcgca ctaccgtcta
5221 aatacgtgaa tttcctgtat ttagcgtccc attacgagaa gttgaaaggt tcacctgaag
5281 ataacgaaca gaagcaactt tttgttgagc agcacaaaca ttatctcgac gaaatcatag
5341 agcaaatttc ggaattcagt aagagagtca tcctagctga tgccaatctg gacaaagtat
5401 taagcgcata caacaagcac agggataaac ccatacgtga gcaggcggaa aatattatcc
5461 atttgtttac tcttaccaac ctcggcgctc cagccgcatt caagtatttt gacacaacga
5521 tagatcgcaa acgatacact tctaccaagg aggtgctaga cgcgacactg attcaccaat
5581 ccatcacggg attatatgaa actcggatag atttgtcaca gcttgggggt gactctggtg
5641 gttctactaa tctgtcagat attattgaaa aggagaccgg taagcaactg gttatccagg
5701 aatccatcct catgctccca gaggaggtgg aagaagtcat tgggaacaag ccggaaagcg
5761 atatactcgt gcacaccgcc tacgacgaga gcaccgacga gaatgtcatg cttctgacta
5821 gcgacgcccc tgaatacaag ccttgggctc tggtcataca ggatagcaac ggtgagaaca
5881 agattaagat gctctctggt ggttctccca agaagaagag gaaagtctaa ccggtcatca
5941 tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
6001 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
6061 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
6121 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
6181 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgat
6241 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
6301 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagccta
6361 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
6421 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
6481 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
6541 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
6601 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
6661 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
6721 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
6781 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
6841 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
6901 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
6961 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
7021 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
7081 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
7141 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
7201 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
7261 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
7321 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
7381 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
7441 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
7501 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
7561 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
7621 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
7681 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
7741 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
7801 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
7861 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
7921 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
7981 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
8041 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
8101 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
8161 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
8221 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
8281 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
8341 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
8401 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc gatctcccga
8461 tcccctaggg tc
//
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