| 质粒名称: | pAAVLINKv1-pCALM1-Cre-CHD9-C-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001741 |
| 产品用途: | 3' AAVLINK plasmid for CHD9 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CHD9 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-CHD9-C-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-CHD9-C-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-CHD9-C-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-CHD9-C-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-CHD9-C-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-CHD9-C-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-CHD9-C-FLAG 7093 bp DNA circular SYN 22-JUL-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7093)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7093
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..141
/label=AAV2 ITR
promoter 156..276
/label=pCALM1
CDS 288..1319
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1320..1360
/label=Splicing Donor
protein_bind 1367..1400
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1401..1492
/label=Splicing Acceptor
/note="Splicing acceptor"
misc_feature 1493..3806
/label=CHD9
misc_feature 1666..1671
/label=SDS
misc_feature 1669..1674
/label=SDS
misc_feature 1672..1677
/label=SDS
misc_feature 1675..1680
/label=SDS
misc_feature 2415..2420
/label=SDS
misc_feature 2494..2499
/label=SDS
misc_feature 3680..3685
/label=SDS
CDS 3813..3878
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3882..4313
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4026..4031
/label=SDS
repeat_region 4356..4496
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4356..4485
/label=AAV2 ITR
rep_origin 4571..5026
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5308..5412
/gene="bla"
/label=AmpR promoter
CDS 5413..6273
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6444..7032
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggggatcag
1501 aatctagttc tgattctgac tcagattctg agagatcatc ttgttcttcc agatcatctt
1561 cttcctcatc atcctcttct tgctcccact ctcgatcagg ctctagttct tcttcatctt
1621 catcttgttc ttcagcatct tcttcatcct cttcctccac ctcttcctcc tcctcctcct
1681 cttcatcttc atcagaagaa agtgacagtg atgaagaaga agcccaaaaa cgagaaagta
1741 ctactcacat gaaagcctat gatgaagaaa gcgtcgcgtc actgagcact acccaggatg
1801 agactcagga tagttttcag atgaacaatg ggacaccaga gtctgcttat atcttacaag
1861 gtggatatat gctggcagcc tcgtattggc caaaggatcg tgtgatgatc aataggttgg
1921 acagtatttg tcaaacagtt ctgaaaggaa agtggccttc agctagaaga agttatgatg
1981 ctaacacagt ggcttctttc tataccacaa aactgctgga cagccctgga gcagctacag
2041 aatacagcga tcccagtgta cccactcccc caggtgccgg tgttaaagaa gaacatgatc
2101 agtcaacaca gatgtcaaag gtgaagaagc atgtacgaga aaaggagttt acagtgaaaa
2161 tcaaagacga aggtggtttg aagttgacat ttcagaagca agggcttgct cagaaaagac
2221 catttgatgg tgaagacggt gctctggggc agcagcagta cctcactcgg cttcgagagc
2281 ttcaaagtgc atcagagacc agcctcgtca atttcccaaa atccatacca gtatcaggta
2341 cttccattca accaaccctt ggtgccaatg gtgtgatatt agacaaccag cctatagtca
2401 aaaaaaggcg aggaaggagg aagaatgtag aaggtgttga catcttcttt tttaacagaa
2461 ataaaccacc taatcatgtt tctttaggct taacctcctc acagatttcc acagggataa
2521 atccagcact atcctatact caacctcaag gaattcctga tacagaaagt ccagttccag
2581 ttattaatct taaagatgga acgagacttg caggagatga tgcaccaaag agaaaggatt
2641 tggaaaaatg gcttaaggag cacccgggtt atgtggaaga tttgggagct tttattccta
2701 gaatgcagct tcatgaggga agacccaaac aaaaaagaca ccgttgcaga aaccccaata
2761 aactagatgt gaatagtctc actggagaag aacgtgttca actgattaac agaagaaatg
2821 ctagaaaggt tggaggtgca tttgctcccc ctttgaaaga tttatgtaga ttcctaaaag
2881 aaaattcaga atatggagta gctcctgaat ggggagatgt tgttaagcaa tctggatttc
2941 ttccagaaag catgtatgaa cgtattctca ctggtcccgt tgtgagagag gaagtaagca
3001 ggcgggggag acggcctaaa agtggaattg caaaggccac agcagcagca gctgctgcat
3061 ctgccaccag tgtttcaggc aatcctttgt tagccaatgg actacttcca ggtgtggatc
3121 tcacaactct tcaggcctta caacaaaacc tacaaaactt gcagtcactg caagtaactg
3181 ctgggttgat gggaatgcct accggccttc cttctggagg agaagctaaa aacatggctg
3241 ctatgttccc catgctgctg tcaggaatgg ctggattacc aaatctgttg ggcatgggag
3301 gactcctgac aaagcctacg gaatctggga cagaagacaa aaagggaagt gactctaagg
3361 agtcagaagg aaaaacagaa aggacagaga gccaaagttc agagaatggt ggagaaaact
3421 ctgtgtcaag ttctccttcc acatcctcta ctgctgcatt aaatacagct gcagctgcca
3481 acccattagc tcttaaccca ctattactat ctaatatact ttatccaggg atgcttctca
3541 ctccaggcct taatcttcat attccaactt tgtcccagtc caatactttt gatgtacaaa
3601 acaaaaacag tgacttaggc tcgtctaagt ctgtagaagt aaaagaagaa gattccagaa
3661 ttaaagatca ggaagacaaa ggaggaactg aaccaagtcc tctcaatgaa aacagcacag
3721 atgagggttc agagaaagct gatgcttcat ctggatctga tagtacatcg tcgtcatctg
3781 aggattcaga ttctagtaat gaagactgta cagactacaa agaccatgac ggtgattata
3841 aagatcatga catcgactac aaggatgacg atgacaagta actcgagata atcaacctct
3901 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
3961 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
4021 tttctcctcc ttgtataaat cctggttagt tcttgccacg gcggaactca tcgccgcctg
4081 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgtttat
4141 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatc tagctttatt tgtgaaattt
4201 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
4261 attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt taaagatctg
4321 gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc
4381 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
4441 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg
4501 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
4561 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
4621 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
4681 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
4741 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
4801 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
4861 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc gggctattct
4921 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
4981 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
5041 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
5101 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
5161 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
5221 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
5281 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
5341 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
5401 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
5461 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
5521 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
5581 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
5641 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
5701 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
5761 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
5821 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
5881 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
5941 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
6001 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
6061 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
6121 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
6181 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
6241 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
6301 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
6361 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
6421 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
6481 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
6541 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
6601 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
6661 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
6721 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
6781 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
6841 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
6901 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
6961 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
7021 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
7081 tttgctcaca tgt
//
