| 质粒名称: | pBAD-HisD-BR1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000235 |
| 产品用途: | Expresses BR1 in E.coli |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pBAD |
| 表达系统: | 细菌表达 |
| 启动子: | araBAD |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | BR1 |
| 物种来源: | Arabidopsis thaliana |
| 密码子优化: | 细菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Enhanced small green fluorescent proteins as a multisensing platform for biosensor development
Liang G., Lai C., Yue Z., Zhang H., Li D., Chen Z., Lu X., Tao L., Subach F.V., Piatkevich K.D.
Front. Bioeng. Biotechnol. 10, 1039317 (2022)
|
买家导航
pBAD-HisD-BR1载体质粒基本信息
pBAD-HisD-BR1质粒图谱载体图谱和pBAD-HisD-BR1载体序列质粒序列多克隆位点信息
pBAD-HisD-BR1质粒载体简介
pBAD-HisD-BR1质粒序列载体序列
LOCUS pBAD-HisD-BR1 4335 bp DNA circular SYN 31-MAY-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4335)
AUTHORS Kiryl Piatkevich
TITLE Direct Submission
JOURNAL Exported Apr 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4335
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 120..285
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction"
primer_bind 250..274
/label=Sequence-F
CDS 328..345
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
misc_feature 355..690
/label=BR1
primer_bind complement(753..777)
/label=Sequence-R
terminator 906..992
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1084..1111
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 1130..1221
/gene="bla"
/label=AmpR promoter
CDS 1222..2082
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2253..2841
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 3027..3167
/label=bom
/note="basis of mobility region from pBR322"
CDS complement(3431..4309)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
ORIGIN
1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
301 taacaggagg aattaaccat ggggagccac catcaccatc accatggcag atctatgatc
361 gagaagaact acgtgatcac cgaccccaga ctgcccgaca accccatcat cttcgccagc
421 gacggcttcc tggagctgac cggctacagc agagaggaga tcctgggcag aaacgccaga
481 ttcctgcagg gccccgagac cgaccaggcc accgtgcaga agatcagaga cgccatcaga
541 gaccagagag agaccaccgt gcagctgatc aactacacca agagcggcag aaagttctgg
601 aacctgctgc acctgcagcc cgtgagagac cagaagggcg agctgcagta cttcatcggc
661 gtgcagctgg acggcagcga cagagtgtaa gaattcgaag cttggctgtt ttggcggatg
721 agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca
781 gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt
841 gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca
901 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
961 tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga
1021 agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta
1081 agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt ttgtttattt
1141 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
1201 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
1261 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
1321 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
1381 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
1441 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
1501 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
1561 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
1621 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
1681 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
1741 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
1801 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
1861 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
1921 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
1981 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
2041 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
2101 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
2161 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
2221 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
2281 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
2341 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
2401 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
2461 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
2521 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
2581 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
2641 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
2701 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
2761 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
2821 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
2881 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
2941 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
3001 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
3061 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
3121 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
3181 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
3241 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
3301 cgaggcagca gatcaattcg cgcgcgaagg cgaagcggca tgcataatgt gcctgtcaaa
3361 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
3421 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
3481 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
3541 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
3601 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
3661 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
3721 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat
3781 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
3841 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
3901 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg
3961 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
4021 aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc
4081 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca
4141 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
4201 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
4261 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
4321 tcccgccatt cagag
//
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