pCDH-3xFLAG-TERT

LOCUS       pCDH-3xFLAG-TERT               11084 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Mammalian expression of 3xFLAG tagged TERT, N-terminal..
ACCESSION   .
VERSION     .
KEYWORDS    pCDH-3xFLAG-TERT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11084)
  AUTHORS   Zhong FL, Batista LF, Freund A, Pech MF, Venteicher AS, Artandi SE
  TITLE     TPP1 OB-fold domain controls telomere maintenance by recruiting 
            telomerase to chromosome ends.
  JOURNAL   Cell. 2012 Aug 3;150(3):481-94. doi: 10.1016/j.cell.2012.07.012.
  PUBMED    22863003
REFERENCE   2  (bases 1 to 11084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11084
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             305..370
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             380..3775
                     /codon_start=1
                     /gene="TERT"
                     /product="human telomerase reverse transcriptase, the 
                     catalytic subunit of telomerase"
                     /label=hTERT
                     /note="prevents telomere shortening and allows 
                     postsenescent cells to proliferate beyond crisis (Counter 
                     et al., 1998); also known as EST2"
                     /translation="PRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPAA
                     FRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALLD
                     GARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVAP
                     SCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGARR
                     RGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEEA
                     TSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQL
                     RPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGNH
                     AQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSPW
                     QVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRDCA
                     WLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFYR
                     KSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVNM
                     DYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFVL
                     RVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHGH
                     VRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRFM
                     CHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFLL
                     VTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPWC
                     GLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDLQ
                     VNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILKA
                     KNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKLP
                     GTTLTALEAAANPALPSDFKTILD"
     promoter        3830..4041
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4054..4322
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4350..5144
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4404..4423)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5014..5033
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    5151..5739
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5204..5224)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5622..5633)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5813..6046
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6118..6239
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6155..6174)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6209..6228
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6279..6414
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6341..6360
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(6447..6463)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6447..6463)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6460..6482)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6471..6487
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6495..6525)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6540..6561
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6678..6695)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6849..7437)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6929..6948)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7608..8468)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8231..8250
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8469..8573)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8641..8659
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8697..8719)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8819..8838
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9032..9054
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9046..9063
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9047..9063
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9078..9304
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9305..9485
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9532..9657
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10150..10383
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10568..10612
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10879..10995
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggttt atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagattc tagagctagc gaattcacca tggggtcccg
      301 ggctgactac aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga
      361 cgatgacaag gggcgcgccc cgcgcgctcc ccgctgccga gccgtgcgct ccctgctgcg
      421 cagccactac cgcgaggtgc tgccgctggc cacgttcgtg cggcgcctgg ggccccaggg
      481 ctggcggctg gtgcagcgcg gggacccggc ggctttccgc gcgctggtgg cccagtgcct
      541 ggtgtgcgtg ccctgggacg cacggccgcc ccccgccgcc ccctccttcc gccaggtgtc
      601 ctgcctgaag gagctggtgg cccgagtgct gcagaggctg tgcgagcgcg gcgcgaagaa
      661 cgtgctggcc ttcggcttcg cgctgctgga cggggcccgc gggggccccc ccgaggcctt
      721 caccaccagc gtgcgcagct acctgcccaa cacggtgacc gacgcactgc gggggagcgg
      781 ggcgtggggg ctgctgctgc gccgcgtggg cgacgacgtg ctggttcacc tgctggcacg
      841 ctgcgcgctc tttgtgctgg tggctcccag ctgcgcctac caggtgtgcg ggccgccgct
      901 gtaccagctc ggcgctgcca ctcaggcccg gcccccgcca cacgctagtg gaccccgaag
      961 gcgtctggga tgcgaacggg cctggaacca tagcgtcagg gaggccgggg tccccctggg
     1021 cctgccagcc ccgggtgcga ggaggcgcgg gggcagtgcc agccgaagtc tgccgttgcc
     1081 caagaggccc aggcgtggcg ctgcccctga gccggagcgg acgcccgttg ggcaggggtc
     1141 ctgggcccac ccgggcagga cgcgtggacc gagtgaccgt ggtttctgtg tggtgtcacc
     1201 tgccagaccc gccgaagaag ccacctcttt ggagggtgcg ctctctggca cgcgccactc
     1261 ccacccatcc gtgggccgcc agcaccacgc gggcccccca tccacatcgc ggccaccacg
     1321 tccctgggac acgccttgtc ccccggtgta cgccgagacc aagcacttcc tctactcctc
     1381 aggcgacaag gagcagctgc ggccctcctt cctactcagc tctctgaggc ccagcctgac
     1441 tggcgctcgg aggctcgtgg agaccatctt tctgggttcc aggccctgga tgccagggac
     1501 tccccgcagg ttgccccgcc tgccccagcg ctactggcaa atgcggcccc tgtttctgga
     1561 gctgcttggg aaccacgcgc agtgccccta cggggtgctc ctcaagacgc actgcccgct
     1621 gcgagctgcg gtcaccccag cagccggtgt ctgtgcccgg gagaagcccc agggctctgt
     1681 ggcggccccc gaggaggagg acacagaccc ccgtcgcctg gtgcagctgc tccgccagca
     1741 cagcagcccc tggcaggtgt acggcttcgt gcgggcctgc ctgcgccggc tggtgccccc
     1801 aggcctctgg ggctccaggc acaacgaacg ccgcttcctc aggaacacca agaagttcat
     1861 ctccctgggg aagcatgcca agctctcgct gcaggagctg acgtggaaga tgagcgtgcg
     1921 ggactgcgct tggctgcgca ggagcccagg ggttggctgt gttccggccg cagagcaccg
     1981 tctgcgtgag gagatcctgg ccaagttcct gcactggctg atgagtgtgt acgtcgtcga
     2041 gctgctcagg tctttctttt atgtcacgga gaccacgttt caaaagaaca ggctcttttt
     2101 ctaccggaag agtgtctgga gcaagttgca aagcattgga atcagacagc acttgaagag
     2161 ggtgcagctg cgggagctgt cggaagcaga ggtcaggcag catcgggaag ccaggcccgc
     2221 cctgctgacg tccagactcc gcttcatccc caagcctgac gggctgcggc cgattgtgaa
     2281 catggactac gtcgtgggag ccagaacgtt ccgcagagaa aagagggccg agcgtctcac
     2341 ctcgagggtg aaggcactgt tcagcgtgct caactacgag cgggcgcggc gccccggcct
     2401 cctgggcgcc tctgtgctgg gcctggacga tatccacagg gcctggcgca ccttcgtgct
     2461 gcgtgtgcgg gcccaggacc cgccgcctga gctgtacttt gtcaaggtgg atgtgacggg
     2521 cgcgtacgac accatccccc aggacaggct cacggaggtc atcgccagca tcatcaaacc
     2581 ccagaacacg tactgcgtgc gtcggtatgc cgtggtccag aaggccgccc atgggcacgt
     2641 ccgcaaggcc ttcaagagcc acgtctctac cttgacagac ctccagccgt acatgcgaca
     2701 gttcgtggct cacctgcagg agaccagccc gctgagggat gccgtcgtca tcgagcagag
     2761 ctcctccctg aatgaggcca gcagtggcct cttcgacgtc ttcctacgct tcatgtgcca
     2821 ccacgccgtg cgcatcaggg gcaagtccta cgtccagtgc caggggatcc cgcagggctc
     2881 catcctctcc acgctgctct gcagcctgtg ctacggcgac atggagaaca agctgtttgc
     2941 ggggattcgg cgggacgggc tgctcctgcg tttggtggat gatttcttgt tggtgacacc
     3001 tcacctcacc cacgcgaaaa ccttcctcag gaccctggtc cgaggtgtcc ctgagtatgg
     3061 ctgcgtggtg aacttgcgga agacagtggt gaacttccct gtagaagacg aggccctggg
     3121 tggcacggct tttgttcaga tgccggccca cggcctattc ccctggtgcg gcctgctgct
     3181 ggatacccgg accctggagg tgcagagcga ctactccagc tatgcccgga cctccatcag
     3241 agccagtctc accttcaacc gcggcttcaa ggctgggagg aacatgcgtc gcaaactctt
     3301 tggggtcttg cggctgaagt gtcacagcct gtttctggat ttgcaggtga acagcctcca
     3361 gacggtgtgc accaacatct acaagatcct cctgctgcag gcgtacaggt ttcacgcatg
     3421 tgtgctgcag ctcccatttc atcagcaagt ttggaagaac cccacatttt tcctgcgcgt
     3481 catctctgac acggcctccc tctgctactc catcctgaaa gccaagaacg cagggatgtc
     3541 gctgggggcc aagggcgccg ccggccctct gccctccgag gccgtgcagt ggctgtgcca
     3601 ccaagcattc ctgctcaagc tgactcgaca ccgtgtcacc tacgtgccac tcctggggtc
     3661 actcaggaca gcccagacgc agctgagtcg gaagctcccg gggacgacgc tgactgccct
     3721 ggaggccgca gccaacccgg cactgccctc agacttcaag accatcctgg actgattaat
     3781 taaggatccg cggccgcaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     3841 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     3901 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     3961 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     4021 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     4081 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     4141 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     4201 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     4261 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     4321 tccaagctgt gaccggcgcc tacgtcgaga tgattgaaca agatggattg cacgcaggtt
     4381 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     4441 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     4501 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     4561 ccgcgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     4621 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     4681 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     4741 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     4801 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     4861 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     4921 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     4981 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     5041 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     5101 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgactcgac aatcaacctc
     5161 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     5221 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     5281 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     5341 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     5401 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     5461 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     5521 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     5581 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     5641 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     5701 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggtaccttt aagaccaatg
     5761 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     5821 ctaattcact cccaacgaag ataagatctg ctttttgctt gtactgggtc tctctggtta
     5881 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     5941 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     6001 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     6061 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     6121 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     6181 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     6241 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     6301 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     6361 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact
     6421 tttgcagaga ccaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg
     6481 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6541 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6601 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6661 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6721 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6781 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6841 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6901 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6961 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7021 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7081 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7141 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7201 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7261 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7321 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7381 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7441 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7501 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7561 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7621 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7681 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7741 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7801 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7861 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7921 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7981 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8041 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8101 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8161 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8221 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8281 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8341 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8401 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8461 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8521 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8581 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     8641 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     8701 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     8761 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     8821 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     8881 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
     8941 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     9001 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     9061 agtgccaagc tgacgcgtgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     9121 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     9181 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     9241 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     9301 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     9361 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     9421 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     9481 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     9541 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     9601 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     9661 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     9721 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     9781 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     9841 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     9901 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     9961 gagcaaaaca aaagtaagac caccgcacag caagcggcca ctgatcttca gacctggagg
    10021 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
    10081 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
    10141 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
    10201 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
    10261 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
    10321 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
    10381 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
    10441 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
    10501 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
    10561 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
    10621 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
    10681 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
    10741 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
    10801 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
    10861 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
    10921 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
    10981 aaaattcaaa attttatcga tactagtatt atgcccagta catgacctta tgggactttc
    11041 ctacttggca gtacatctac gtattagtca tcgctattac catg
//