pGTQL1221YC

LOCUS       pGTQL1221YC             12028 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Gateway compatible BiFC vector (pEarleyGate202-YC).
ACCESSION   .
VERSION     .
KEYWORDS    pGTQL1221YC
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12028)
  AUTHORS   Lu Q, Tang X, Tian G, Wang F, Liu K, Nguyen V, Kohalmi SE, Keller 
            WA, Tsang EW, Harada JJ, Rothstein SJ, Cui Y
  TITLE     Arabidopsis homolog of the yeast TREX-2 mRNA export complex: 
            components and anchoring nucleoporin.
  JOURNAL   Plant J. 2010 Jan;61(2):259-70. doi: 
            10.1111/j.1365-313X.2009.04048.x. Epub 2009 Oct 16.
  PUBMED    19843313
REFERENCE   2  (bases 1 to 12028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12028
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      111..363
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        2306..2651
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2594..2614
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    2681..2735
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             2740..2763
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    2767..2891
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2916..2946
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3000..3659
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3066..3085)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4001..4306
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRIVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4185..4204
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4347..4471)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     4658..4679
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      4800..5507
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(5532..5549)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5532..5548)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5541..5563)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5751..5775
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7075..7704
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8138..9205
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      9271..9465
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9701..9723)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9809..9949
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9864..9883
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9964..9981)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10135..10723)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10215..10234)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10810..11604)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
     1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
     1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
     1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
     1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
     1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
     1681 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt
     1741 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac
     1801 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
     1861 tagagaggcc tacgcagcag gtctcatcaa gacgatctac ccgagtaaca atctccagga
     1921 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat
     1981 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat
     2041 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt
     2101 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac
     2161 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga
     2221 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata
     2281 cagtctcaga agaccaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc
     2341 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     2401 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg
     2461 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     2521 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     2581 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     2641 tggagaggac acgctcgagt ataagagctc tatttttaca acaattacca acaacaacaa
     2701 acaacaaaca acattacaat tacatttaca attaccatgg actacaaaga cgatgacgac
     2761 aaaatcacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
     2821 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
     2881 cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
     2941 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa
     3001 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     3061 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     3121 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     3181 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     3241 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     3301 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     3361 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     3421 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     3481 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     3541 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     3601 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa
     3661 cgcgtggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt
     3721 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat
     3781 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat
     3841 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt
     3901 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa
     3961 atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac
     4021 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
     4081 gcccgggcga cggatagtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
     4141 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
     4201 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
     4261 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct
     4321 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt
     4381 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
     4441 tttctcgttc agctttcttg tacaaagtgg tgcctaggcc tgcttgtaag atccctaacg
     4501 acctcaagca gaaagtcatg aaccacggca gcgtgcagct cgccgaccac taccagcaga
     4561 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt
     4621 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     4681 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtgacctagg tgagtctaga
     4741 gagttaattc taggtgagtc tagagagtta attaagaccc gggactagtc cctagagtcc
     4801 tgctttaatg agatatgcga gacgcctatg atcgcatgat atttgctttc aattctgttg
     4861 tgcacgttgt aaaaaacctg agcatgtgta gctcagatcc ttaccgccgg tttcggttca
     4921 ttctaatgaa tatatcaccc gttactatcg tatttttatg aataatattc tccgttcaat
     4981 ttactgattg taccctacta cttatatgta caatattaaa atgaaaacaa tatattgtgc
     5041 tgaataggtt tatagcgaca tctatgatag agcgccacaa taacaaacaa ttgcgtttta
     5101 ttattacaaa tccaatttta aaaaaagcgg cagaaccggt caaacctaaa agactgatta
     5161 cataaatctt attcaaattt caaaagtgcc ccaggggcta gtatctacga cacaccgagc
     5221 ggcgaactaa taacgctcac tgaagggaac tccggttccc cgccggcgcg catgggtgag
     5281 attccttgaa gttgagtatt ggccgtccgc tctaccgaaa gttacgggca ccattcaacc
     5341 cggtccagca cggcggccgg gtaaccgact tgctgccccg agaattatgc agcatttttt
     5401 tggtgtatgt gggccccaaa tgaagtgcag gtcaaacctt gacagtgacg acaaatcgtt
     5461 gggcgggtcc agggcgaatt ttgcgacaac atgtcgaggc tcagcaggac ctgcaggcat
     5521 gcaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     5581 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     5641 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatgcta gagcagcttg
     5701 agcttggatc agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt tgacaggata
     5761 tattggcggg taaacctaag agaaaagagc gtttattaga ataacggata tttaaaaggg
     5821 cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc
     5881 gggatcaaag tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt
     5941 cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg
     6001 ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata
     6061 cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg
     6121 gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg
     6181 cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg
     6241 agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag
     6301 accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg
     6361 gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca
     6421 tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca
     6481 cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc
     6541 tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga
     6601 aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca
     6661 gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga
     6721 ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc
     6781 gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat
     6841 cgcggccggg tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat
     6901 cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga
     6961 aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc
     7021 ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag
     7081 gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta
     7141 gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc
     7201 agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac
     7261 cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac
     7321 ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg
     7381 attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt
     7441 aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg
     7501 atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc
     7561 attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca
     7621 accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct
     7681 gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa
     7741 acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc
     7801 tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca
     7861 agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca
     7921 tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct
     7981 aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg
     8041 tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgtctgccg gccctgcaat
     8101 ggcactggaa cccccaagcc cgaggaatcg gcgtgacggt cgcaaaccat ccggcccggt
     8161 acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc
     8221 ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga
     8281 tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc
     8341 gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg
     8401 cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc
     8461 tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc
     8521 cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga
     8581 atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca
     8641 cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct
     8701 ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc
     8761 caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat
     8821 cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta
     8881 ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt
     8941 gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc
     9001 agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa
     9061 gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt
     9121 gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga
     9181 gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc
     9241 aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa
     9301 gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg
     9361 gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc
     9421 tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg
     9481 cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc
     9541 cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg
     9601 caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca
     9661 aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     9721 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     9781 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     9841 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     9901 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     9961 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    10021 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    10081 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    10141 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    10201 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    10261 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    10321 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    10381 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    10441 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    10501 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    10561 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    10621 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    10681 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    10741 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgca
    10801 ttctaggtac taaaacaatt catccagtaa aatataatat tttattttct cccaatcagg
    10861 cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga tatcctccct
    10921 gatcgaccgg acgcagaagg caatgtcata ccacttgtcc gccctgccgc ttctcccaag
    10981 atcaataaag ccacttactt tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc
    11041 gtgggaaaag acaagttcct cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg
    11101 cggatcttta aatggagtgt cttcttccca gttttcgcaa tccacatcgg ccagatcgtt
    11161 attcagtaag taatccaatt cggctaagcg gctgtctaag ctattcgtat agggacaatc
    11221 cgatatgtcg atggagtgaa agagcctgat gcactccgca tacagctcga taatcttttc
    11281 agggctttgt tcatcttcat actcttccga gcaaaggacg ccatcggcct cactcatgag
    11341 cagattgctc cagccatcat gccgttcaaa gtgcaggacc tttggaacag gcagctttcc
    11401 ttccagccat agcatcatgt ccttttcccg ttccacatca taggtggtcc ctttataccg
    11461 gctgtccgtc atttttaaat ataggttttc attttctccc accagcttat ataccttagc
    11521 aggagacatt ccttccgtat cttttacgca gcggtatttt tcgatcagtt ttttcaattc
    11581 cggtgatatt ctcattttag ccatttatta tttccttcct cttttctaca gtatttaaag
    11641 ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa
    11701 aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc gtataacata
    11761 gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt
    11821 tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg
    11881 ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc
    11941 cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct
    12001 gccggtcggg gagctgttgg ctggctgg
//