| 质粒名称: | phCMV-coGALVenv-C4070A |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 172217 |
| 存储实验室: | Boris Fehse |
|
载体骨架基本信息 |
|
| 载体名称: | phCMV |
| 载体出品公司: | Clontech |
| 空载体大小: | 4816bp |
| 完整质粒大小: | 6892bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | coGALVenv |
| 物种来源: | Gibbon Ape Leukemia Virus |
| 插入大小: | 2076bp |
| 基因突变: | Carries C-terminus of amphotropic MLV strain 4070A to increase packaging efficiency |
| 启动子: | CMV immediate early promoter |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BamHI (not destroyed) |
| 3’克隆位点: | BamHI (not destroyed) |
| 5’测序引物 |
TCCTACAGCTCCTGGGCAAC
(Common Sequencing Primers)
|
|
文献引用方法 |
|
| 材料和方法部分: | phCMV-coGALVenv-C4070A was a gift from Boris Fehse (Addgene plasmid # 172217 ; http://n2t.net/addgene:172217 ; RRID:Addgene_172217) |
| 参考文献部分: | Efficient Pseudotyping of Different Retroviral Vectors Using a Novel, Codon-Optimized Gene for Chimeric GALV Envelope. Mirow M, Schwarze LI, Fehse B, Riecken K. Viruses. 2021 Jul 27;13(8). pii: v13081471. doi: 10.3390/v13081471. 10.3390/v13081471 Pu |
买家导航
phCMV-coGALVenv-C4070A载体质粒基本信息
phCMV-coGALVenv-C4070A质粒图谱载体图谱和phCMV-coGALVenv-C4070A载体序列质粒序列多克隆位点信息
phCMV-coGALVenv-C4070A质粒载体简介
phCMV-coGALVenv-C4070A质粒序列载体序列
LOCUS phCMV-coGALVenv-C4070A 6891 bp DNA circular SYN 25-AUG-2024
DEFINITION Expresses the envelope protein of Gibbon Ape Leukemia Virus (GALV)
for pseudotyping of retroviral or lentiviral vectors.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6891)
AUTHORS Mirow M, Schwarze LI, Fehse B, Riecken K
TITLE Efficient Pseudotyping of Different Retroviral Vectors Using a
Novel, Codon-Optimized Gene for Chimeric GALV Envelope.
JOURNAL Viruses. 2021 Jul 27;13(8). pii: v13081471. doi: 10.3390/v13081471.
PUBMED 34452336
REFERENCE 2 (bases 1 to 6891)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Viruses.";
date: "2021-07-27"; volume: "13(8). pii: v13081471. doi"; pages: "
10.3390/v13081471"
FEATURES Location/Qualifiers
source 1..6891
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 83..462
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 463..666
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 617..637
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 663..687
/label=LNCX
/note="Human CMV promoter, forward primer"
intron 778..1350
/label=beta-globin intron
/note="intron from rabbit beta-globin gene"
regulatory 1417..1426
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
primer_bind complement(3582..3601)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 3647..3702
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(3701..3720)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(4060..4076)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4060..4076)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4073..4095)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4084..4100
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4108..4138)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4153..4174
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4291..4308)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4462..5050)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4542..4561)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5221..6081)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5844..5863
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6082..6186)
/gene="bla"
/label=AmpR promoter
rep_origin complement(6212..6667)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6349..6370)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 6561..6580
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6794..6816
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 6808..6825
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 6809..6825
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 6835..6854
/label=T7
/note="T7 promoter, forward primer"
promoter 6835..6853
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 gagcttggcc cattgcatac gttgtatcca tatcataata tgtacattta tattggctca
61 tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt
121 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
301 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
361 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
421 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
661 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
721 catagaagac accgggaccg atccagcctc cggtcgaccg atcctgagaa cttcagggtg
781 agtttgggga cccttgattg ttctttcttt ttcgctattg taaaattcat gttatatgga
841 gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga
901 ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt
961 attttctttt cattttctgt aactttttcg ttaaacttta gcttgcattt gtaacgaatt
1021 tttaaattca cttttgttta tttgtcagat tgtaagtact ttctctaatc actttttttt
1081 caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac aattgttata
1141 attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt
1201 attggtagaa acaactacac cctggtcatc atcctgcctt tctctttatg gttacaatga
1261 tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac
1321 catgttcatg ccttcttctc tttcctacag ctcctgggca acgtgctggt tgttgtgctg
1381 tctcatcatt ttggcaaaga attcctcgac ggatccgcca ccatggtttt gctgcctggc
1441 tctatgctgc tgaccagcaa cctgcaccac ctgagacacc agatgagccc tggcagctgg
1501 aagcggctga tcatcctgct gagctgtgtg tttggcggcg gaggcacaag cctgcagaac
1561 aagaatcccc accagcctat gacactgacc tggcaggtcc tgtctcagac cggggatgtc
1621 gtgtgggata caaaggctgt gcagcctcct tggacctggt ggcctacact gaagcctgat
1681 gtgtgtgccc tggccgcctc tctggaatct tgggatattc ctggcaccga cgtgtccagc
1741 agcaagagag tcagacctcc tgacagcgat tacaccgccg cctacaagca gatcacatgg
1801 ggcgccatcg gctgcagcta ccctagagcc agaaccagaa tggccagcag caccttctac
1861 gtgtgcccca gagatggcag aaccctgagc gaggctagaa gatgtggcgg cctggaaagc
1921 ctgtactgca aagagtggga ctgcgagaca accggcacag gctactggct gagcaagagc
1981 agcaaggacc tgatcaccgt gaagtgggac cagaacagcg agtggaccca gaaattccag
2041 cagtgccacc agacaggctg gtgcaaccct ctgaagatcg acttcaccga caagggcaag
2101 ctgtccaagg attggatcac cggcaagacc tggggcctgc gcttctatgt gtctggacat
2161 cctggcgtgc agttcaccat ccggctgaag atcaccaaca tgcccgctgt ggctgtgggc
2221 cctgatctgg tgcttgttga acagggccct cctagaacct ctctggcttt gcctcctcca
2281 ctgcctccta gagaagcccc tccacctagc ctgcctgaca gcaattctac agccctggcc
2341 accagcgctc agacccctac agtgcggaaa accatcgtga ccctgaacac ccctcctcca
2401 accaccggcg acagactgtt tgatctggtc cagggcgcct tcctgacact gaacgctaca
2461 aatcctggcg ccaccgagag ctgttggctg tgtcttgcta tgggcccacc ttactacgag
2521 gccattgcct ctagcggcga ggtggcctat agcaccgacc tggatagatg cagatggggc
2581 acccagggaa agctgaccct gacagaagtt tctggccacg gcctgtgcat cggcaaggtg
2641 ccctttacac accagcacct gtgcaatcag accctgagca tcaacagcag cggcgaccac
2701 cagtacctgc tgcctagcaa tcattcttgg tgggcctgca gcaccggcct gacaccttgt
2761 ctgagcacca gcgtgttcaa ccagaccagg gacttctgca tccaagtgca gctgatcccc
2821 aggatctact actaccccga ggaagtgctg ctccaggcct acgacaattc tcaccctcgg
2881 accaagcggg aagccgtgtc tctgacactg gctgtgctgc tcggactggg aatcacagcc
2941 ggaatcggaa caggcagcac tgccctgatc aagggcccta tcgatctgca gcagggactg
3001 acctctctgc agattgccat cgacgccgat ctgagagccc tgcaggatag cgtgtccaag
3061 ctggaagata gcctgaccag cctgagcgaa gtcgtgctgc agaatagaag aggcctggac
3121 ctgctgttcc tgaaagaagg cggactttgc gccgctctga aagaggaatg ctgtttctac
3181 atcgaccact ctggcgccgt gcgggacagc atgaagaagc tgaaagagaa gctggacaag
3241 cgccagctgg aaagacagaa gtctcagaat tggtacgaag gctggttcaa cacaagcccc
3301 tggttcacca cactgatcag caccatcatg ggccctctga tcgtgctgct gctgatcctg
3361 ctgtttggcc cctgcatcct gaacagactg gtgcagttcg tgaaggacag aatcagcgtc
3421 gtgcaggccc tggtgctgac acagcagtat caccagctga agcctctgga atacgagccc
3481 tgataaggat ccgtcgagga attcactcct caggtgcagg ctgcctatca gaaggtggtg
3541 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
3601 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
3661 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
3721 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
3781 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag
3841 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt
3901 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta
3961 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta
4021 tggagatccc tcgacggatc ggccgcaatt cgtaatcatg tcatagctgt ttcctgtgtg
4081 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
4141 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
4201 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
4261 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
4321 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
4381 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
4441 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
4501 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
4561 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
4621 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
4681 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
4741 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
4801 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
4861 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
4921 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
4981 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
5041 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
5101 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
5161 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
5221 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
5281 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
5341 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
5401 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
5461 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
5521 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
5581 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
5641 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
5701 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
5761 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
5821 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
5881 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
5941 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
6001 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
6061 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
6121 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
6181 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
6241 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
6301 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
6361 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
6421 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
6481 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
6541 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
6601 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
6661 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
6721 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
6781 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
6841 gactcactat agggcgaatt ggagctccac cgcggtggcg gccgctctag a
//
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