| 质粒名称: | Xlone-Gem(1-100)-mScarlet(R198Q)-IRES-mScarlet(Y84W)-hCdt1(30-120) |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001816 |
| 产品用途: | For constructing a tr-Fucci(SA)-red stably transfected cell line in mammalian cells. |
| 存储实验室: | Xin Zhang Lab |
|
载体骨架基本信息 |
|
| 载体名称: | XLone |
| 表达系统: | 哺乳动物表达 |
| 启动子: | TRE3GS |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | Gem(1-100)-mScarlet(R198Q), mScarlet(Y84W)-hCdt1(30-120) |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Time-resolved fluorescent proteins expand fluorescent microscopy in temporal and spectral domains.
Tan Z., Hsiung C-H., Feng J., Zhang Y., Wan Y., Chen J., Sun K., Lu P., Zang J., Yang W., Gao Y., Yin J., Zhu T., Lu Y., Pan Z., Zou Y., Liao C., Li X., Ye Y., Liu Y., Zhang X.
Cell 0, (2025)
|
Xlone-Gem(1-100)-mScarlet(R198Q)-IRES-mScarlet(Y84W)-hCdt1(3
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
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Xlone-Gem(1-100)-mScarlet(R198Q)-IRES-mScarlet(Y84W)-hCdt1(3载体质粒基本信息
Xlone-Gem(1-100)-mScarlet(R198Q)-IRES-mScarlet(Y84W)-hCdt1(3质粒图谱载体图谱和Xlone-Gem(1-100)-mScarlet(R198Q)-IRES-mScarlet(Y84W)-hCdt1(3载体序列质粒序列多克隆位点信息
Xlone-Gem(1-100)-mScarlet(R198Q)-IRES-mScarlet(Y84W)-hCdt1(3质粒载体简介
Xlone-Gem(1-100)-mScarlet(R198Q)-IRES-mScarlet(Y84W)-hCdt1(3质粒序列载体序列
LOCUS Xlone-Gem(1-100)-mScarlet(R198Q)-IRES-mScarlet(Y84W)-hCdt1(30-120) 8331 bp DNA circular SYN 28-JUL-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8331)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Aug 13, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8331
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 9..376
/label=TRE3GS promoter
/note="3rd-generation Tet-responsive promoter that can be
activated by binding of Tet-On(R) 3G, modified to eliminate
binding sites for endogenous mammalian transcription
factors"
protein_bind 17..35
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 53..71
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 89..107
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 125..143
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 161..179
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 197..215
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 233..251
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
regulatory 403..412
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 409..1104
/codon_start=1
/product="bright monomeric red fluorescent protein evolved
from a synthetic template (Bindels et al., 2016)"
/label=mScarlet
/translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDQKLDITSHNEDYTVVEQYERSEGRH
STGGMDELYK"
misc_feature 799..804
/label=SDS
CDS 1135..1464
/codon_start=1
/product="degron consisting of residues 1-110 of human
Geminin (Zielke and Edgar, 2015)"
/label=Gem1 (1-110)
/translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
KRRKAL"
CDS 2810..3082
/codon_start=1
/product="degron consisting of residues 30-120 of human
Cdt1 (Zielke and Edgar, 2015)"
/label=Cdt1 (30-120)
/translation="PSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSV
DEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTI"
misc_feature 2879..2884
/label=SDS
misc_feature 3044..3049
/label=SDS
misc_feature 3194..3199
/label=SDS
polyA_signal 3293..3427
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3748..3764)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 4238..4342
/gene="bla"
/label=AmpR promoter
CDS 4343..5203
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
misc_feature 5204..6144
/label=ColE1
/note="ColE1"
rep_origin 5374..5962
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 6250..6271
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6286..6316
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6324..6340
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 6348..6364
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
polyA_signal 6628..6709
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
CDS complement(6826..7224)
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
CDS complement(7225..7281)
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS complement(7291..8034)
/codon_start=1
/product="modified rtTA protein that binds tightly to
promoters containing the tet operator in the presence of
doxycycline"
/label=Tet-On(R) 3G
/translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
DALDDFDLDMLPADALDDFDLDMLPG"
CDS complement(8035..8058)
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
regulatory 8058..8067
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
promoter complement(8087..8298)
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
ORIGIN
1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
241 agtgatagag aacgtataag ctttgcttat gtaaaccagg gcgcctataa aagagtgctg
301 attttttgag taaacttcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
361 ttcctaccct cgtaaaggta ccttggtacc gagctcggat ccgccaccat ggtgagcaag
421 ggcgaggcag tgatcaagga gttcatgcgg ttcaaggtgc acatggaggg ctccatgaac
481 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
541 gccaagctga aggtgaccaa gggtggcccc ctgcccttct cctgggacat cctgtcccct
601 cagttcatgt acggctccag ggccttcacc aagcaccccg ccgacatccc cgactactat
661 aagcagtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
721 gccgtgaccg tgacccagga cacctccctg gaggacggca ccctgatcta caaggtgaag
781 ctccgcggca ccaacttccc tcctgacggc cccgtaatgc agaagaagac aatgggctgg
841 gaagcgtcca ccgagcggtt gtaccccgag gacggcgtgc tgaagggcga cattaagatg
901 gccctgcgcc tgaaggacgg cggccgctac ctggcggact tcaagaccac ctacaaggcc
961 aagaagcccg tgcagatgcc cggcgcctac aacgtcgacc agaagttgga catcacctcc
1021 cacaacgagg actacaccgt ggtggaacag tacgaacgct ccgagggccg ccactccacc
1081 ggcggcatgg acgagctgta caagggctac cccagccact ggcggcctct ggaaatgaac
1141 cccagcatga agcagaagca ggaagagatc aaagagaaca tcaagaacag cagcgtgccc
1201 aggcggaccc tgaagatgat ccagcccagc gccagcggat ctctggtggg ccgggagaat
1261 gagctgtctg ccggcctgag caagcggaag caccggaacg accacctgac cagcaccacc
1321 tctagcccag gcgtgatcgt gcccgagagc agcgagaaca agaacctggg cggcgtgacc
1381 caagaaagct tcgacctgat gatcaaagaa aaccccagca gccagtactg gaaagaggtg
1441 gccgagaagc ggcggaaggc cctgtaaggt accgagctcg gatcctactc cggtattgcg
1501 gtacccttgt acgcctgttt tatactccct tcccgtaact tagacgcaca aaaccaagtt
1561 caatagaagg gggtacaaac cagtaccacc acgaacaagc acttctgttt ccccggtgat
1621 gtcgtataga ctgcttgcgt ggttgaaagc gacggatccg ttatccgctt atgtacttcg
1681 agaagcccag taccacctcg gaatcttcga tgcgttgcgc tcagcactca accccagagt
1741 gtagcttagg ctgatgagtc tggacatccc tcaccggtga cggtggtcca ggctgcgttg
1801 gcggcctacc tatggctaac gccatgggac gctagttgtg aacaaggtgt gaagagccta
1861 ttgagctaca taagaatcct ccggcccctg aatgcggcta atcccaacct cggagcaggt
1921 ggtcacaaac cagtgattgg cctgtcgtaa cgcgcaagtc cgtggcggaa ccgactactt
1981 tgggtgtccg tgtttccttt tattttattg tggctgctta tggtgacaat cacagattgt
2041 tatcataaag cgaattggat actagtccag tgtggtggaa atggtcagta aaggggaagc
2101 cgtcattaag gagtttatgc gcttcaaggt tcatatggaa ggtagtatga atggccacga
2161 attcgagatt gagggggagg gggaggggag accatacgag gggactcaga cagctaaact
2221 gaaggtgact aagggcggac cattgccctt ctcatgggac atcctgagtc ctcagtttat
2281 gtatggatcc cgggcattca caaaacatcc tgctgatatc ccagactatt ggaaacagag
2341 cttccccgaa ggttttaagt gggaaagagt gatgaacttc gaagatggtg gggccgtcac
2401 tgtcactcaa gacacctctc tggaagacgg caccctgatc tacaaagtta aactgcgggg
2461 aactaacttc cctccagatg ggcccgtcat gcagaaaaaa accatgggat gggaggcaag
2521 caccgaaaga ctgtatccag aggacggagt tctgaaaggc gatatcaaga tggccctccg
2581 cttgaaggac ggaggaagat atctggcaga cttcaaaact acctacaaag caaaaaagcc
2641 tgtccagatg cctggtgcat acaatgtcga cagaaagttg gacatcacta gtcataacga
2701 agactataca gtcgttgagc agtacgagag gagcgagggg aggcactcca ccgggggtat
2761 ggatgagctg tataaggggc atggcacggg ctcgggcacc gcctcgtcgc cctctcctgc
2821 cagacctgcc ctgagagccc ctgcctccgc cacctccggc agcagaaaga gagccagacc
2881 tcctgccgcc cctggcagag atcaggccag gccccctgcc cgcagacggc tccggctgtc
2941 cgtggatgag gtgtccagcc ctagcacccc cgaggccccc gatatccctg cctgtcccag
3001 ccctggccag aaaatcaaga agagcacacc tgccgccgga cagcctcctc acctgaccag
3061 cgcccaggac caggacacca tctaagaatt ctgcagatat ccagcacagt ggactagtag
3121 accacctccc ctgcgagcta agctggacag ccaatgacgg gtaagagagt gacatttttc
3181 actaacctaa gacaggaggg ccgtcagagc tactgcctaa tccaaagacg ggtaaaagtg
3241 ataaaaatgt atcactccaa cctaagacag gcgcagcttc cgagggattt gagatccaga
3301 catgataaga tacattgatg agtttggaca aaccaaaact agaatgcagt gaaaaaaatg
3361 ccttatttgt gaaatttgtg atgctattgc cttatttgta accattataa gctgcaataa
3421 acaagtttga tatctataac aagaaaatat atatataata agttatcacg taagtagaac
3481 atgaaataac aatataatta tcgtatgagt taaatcttaa aagtcacgta aaagataatc
3541 atgcgtcatt ttgactcacg cggtcgttat agttcaaaat cagtgacact taccgcattg
3601 acaagcacgc ctcacgggag ctccaagcgg cgactgagat gtcctaaatg cacagcgacg
3661 gattcgcgct atttagaaag agagagcaat atttcaagaa tgcatgcgtc aattttacgc
3721 agactatctt tctagggtta agaattcact ggccgtcgtt ttacaacgtc gtgactggga
3781 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
3841 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
3901 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
3961 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
4021 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
4081 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
4141 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
4201 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
4261 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
4321 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
4381 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
4441 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
4501 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
4561 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
4621 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
4681 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
4741 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
4801 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
4861 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
4921 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
4981 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
5041 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
5101 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
5161 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
5221 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
5281 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
5341 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
5401 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
5461 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
5521 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
5581 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
5641 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
5701 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
5761 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
5821 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
5881 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
5941 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
6001 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
6061 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
6121 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
6181 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
6241 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
6301 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
6361 tgaccatgat tacgccaagg tcgacttaac cctagaaaga taatcatatt gtgacgtacg
6421 ttaaagataa tcatgcgtaa aattgacgca tgtgttttat cggtctgtat atcgaggttt
6481 atttattaat ttgaatagat attaagtttt attatattta cacttacata ctaataataa
6541 attcaacaaa caatttattt atgtttattt atttattaaa aaaaaacaaa aactcaaaat
6601 ttcttctata aagtaacaaa acttttagca gtgaaaaaaa tgctttattt gtgaaatttg
6661 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
6721 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
6781 aaacctctac aaatgtggta tggctgatta tgatcctctg gagatttagc cctcccacac
6841 ataaccagag ggcagcaatt cacgaatccc aactgccgtc ggctgtccat cactgtcctt
6901 cactatggct ttgatcccag gatgcagatc gagaagcacc tgtcggcacc gtccgcaggg
6961 gctcaagatg cccctgttct catttccgat cgcgacgata caagtcaggt tgccagctgc
7021 cgcagcagca gcagtgccca gcaccacgag ttctgcacaa ggtcccccag taaaatgata
7081 tacattgaca ccagtgaaga tgcggccgtc gctagagaga gctgcgctgg cgacgctgta
7141 gtcttcagag atggggatgc tgttgattgt agccgttgct ctttcaatga gggtggattc
7201 ttcttgagac aaaggcttgg ccatggggcc ggggttctcc tccacgtcgc cggcctgctt
7261 cagcaggctg aagttggtgg cgccgctgcc cccggggagc atgtcaaggt caaaatcgtc
7321 aagagcgtca gcaggcagca tatcaaggtc aaagtcgtca agggcatcgg ctgggagcat
7381 gtctaagtca aaatcgtcaa gggcgtcggt cggcccgccg ctttcgcact ttagctgttt
7441 ctccaggcca catatgatta gttccaggcc gaaaaggaag gcaggttcgg ctccctgccg
7501 gtcgaacagc tcaattgctt gtttcagaag tgggggcata gaatcggtgg taggtgtctc
7561 tctttcctct tttgctactt gatgctcctg ttcctccaat acgcagccca gtgtaaagtg
7621 gcccacggcg gacagagcgt acagtgcgtt ctccagggag aagccttgct gacacaggaa
7681 cgcgagctga ttttccaggg tttcgtactg tttctctgtt gggcgggtgc cgagatgcac
7741 tttagccccg tcgcgatgtg agaggagagc acagcggtat gacttggcgt tgttccgcag
7801 aaagtcttgc catgactcgc cttccagggg gcaggagtgg gtatgatgcc tgtccagcat
7861 ctcgattggc agggcatcga gcagggcccg cttgttcttc acgtgccagt acagggtagg
7921 ctgctcaact cccagctttt gagcgagttt ccttgtcgtc aggccttcga taccgactcc
7981 attgagtaat tccagagcag agtttatgac tttgctcttg tccagtctag acatcttatc
8041 gtcatcgtct ttgtaatcca tggtggcgga tcccgcgtca cgacacctgt gttctggcgg
8101 caaacccgtt gcgaaaaaga acgttcacgg cgactactgc acttatatac ggttctcccc
8161 caccctcggg aaaaaggcgg agccagtaca cgacatcact ttcccagttt accccgcgcc
8221 accttctcta ggcaccggtt caattgccga cccctccccc caacttctcg gggactgtgg
8281 gcgatgtgcg ctctgcccac tgacgggcac cggagccact cgagtggaat t
//
