| 质粒名称: | pAAV-CaMKII-Ace-mNeon2-Kv2.1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000564 |
| 产品用途: | to express Ace-mNeon2-Kv2.1 in mamalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: |
CaMKII
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | Ace-mNeon2-Kv2.1 |
| 物种来源: | mouse |
| 密码子优化: | 老鼠表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Bright and Photostable Voltage Sensors Derived from mBaoJin
Zhang H., Zhou S., Kuzmicheva T.P., Subach O.M., Grimm C., Eom M., Baror-Sebban S., Adam Y., Yoon Y.G., Emilliani V., Subach F.V., Piatkevich K.D.
bioRxiv 2025.05.30.657123 (2025)
|
买家导航
pAAV-CaMKII-Ace-mNeon2-Kv2.1载体质粒基本信息
pAAV-CaMKII-Ace-mNeon2-Kv2.1质粒图谱载体图谱和pAAV-CaMKII-Ace-mNeon2-Kv2.1载体序列质粒序列多克隆位点信息
pAAV-CaMKII-Ace-mNeon2-Kv2.1质粒载体简介
pAAV-CaMKII-Ace-mNeon2-Kv2.1质粒序列载体序列
LOCUS pAAV-CaMKII-Ace-mNeon2-Kv2.1 6726 bp DNA circular SYN 24-JUN-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6726)
AUTHORS Kiryl Piatkevich
TITLE Direct Submission
JOURNAL Exported Apr 29, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..6726
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 34..223
/label=ITR
promoter 237..1526
/label=CaMKII promoter
/note="promoter for the mouse calcium/calmodulin-dependent
kinase II alpha subunit"
primer_bind 1512..1564
/label=1104-1-Ace-F
regulatory 1539..1548
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
regulatory 1539..1544
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1545..2231
/codon_start=1
/label=AceNR
/translation="MADVETETGMIAQWIVFAIMAAAAIAFGVAVHFRPSELKSAYYIN
IAICTIAATAYYAMAVNYQDLTMNGERQVVYARYISWVLTTPLLLLDLIVMTKMGGVMI
SWVIGADIFMIVFGILGAFEDEHKFKWVYFIAGCVMQAVLTYGMYNATWKDDLKKSPEY
HSSYVSLLVFLSILWVFYPVVWAFGSGSGVLSVDNEAILMGILDVLAKPLFGMGCLIAH
ETIFKSY"
primer_bind complement(2202..2231)
/label=0806-1-Ace-PR-R
primer_bind 2212..2255
/label=1104-2-mNeonGreen-F
CDS 2232..2900
/codon_start=1
/product="bright monomeric yellow-green fluorescent protein
derived from LanYFP (Shaner et al., 2013)"
/label=mNeonGreen
/translation="PATHELHIFGSINGVDFDMVGQGTGNPNDGYEELNLKSTKGDLQF
SPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGASLTVNYRYTYE
GSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIISTFKWSYTTGNGKR
YRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQKAFTDVMGMDELY
K"
regulatory 2448..2457
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
primer_bind complement(2877..2920)
/label=1104-2-mNeonGreen-R
CDS 2901..2960
/codon_start=1
/label=KGC
/translation="KSRITSEGEYIPLDQIDINV"
primer_bind 2901..2920
/label=1104-3-KGC-F
CDS 2961..2981
/codon_start=1
/label=ER2
/translation="FCYENEV"
CDS 2982..3176
/codon_start=1
/label=Kv2.1
/translation="QSQPILNTKEMAPQSKPPEELEMSSMPSPVAPLPARTEGVIDMRS
MSSIDSFISCATDFPEATRF"
primer_bind complement(3157..3205)
/label=1104-3-Kv2.1-R
primer_bind complement(3157..3178)
/label=Kv2.1-Rv-ER2
misc_feature 3204..3792
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(3675..3686)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3835..3956
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature complement(4059..4248)
/label=ITR
misc_feature 4302..5242
/label=ColE1
/note="ColE1"
CDS complement(5243..6103)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6104..6208)
/gene="bla"
/label=AmpR promoter
rep_origin complement(6234..6689)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 gcgatcggtg cgggcctctt cgctattacg ccagctgcgc gctcgctcgc tcactgaggc
61 cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg
121 agcgcgcaga gagggagtgg ccaactccat cactaggggt tccttgtagt taatgattaa
181 cccgccatgc tacttatcta cgtagccatg ctctaggaag atcgtaggtt aattaacatt
241 atggccttag gtcacttcat ctccatgggg ttcttcttct gattttctag aaaatgagat
301 gggggtgcag agagcttcct cagtgacctg cccagggtca catcagaaat gtcagagcta
361 gaacttgaac tcagattact aatcttaaat tccatgcctt gggggcatgc aagtacgata
421 tacagaagga gtgaactcat tagggcagat gaccaatgag tttaggaaag aagagtccag
481 ggcagggtac atctacacca cccgcccagc cctgggtgag tccagccacg ttcacctcat
541 tatagttgcc tctctccagt cctaccttga cgggaagcac aagcagaaac tgggacagga
601 gccccaggag accaaatctt catggtccct ctgggaggat gggtggggag agctgtggca
661 gaggcctcag gaggggccct gctgctcagt ggtgacagat aggggtgaga aagcagacag
721 agtcattccg tcagcattct gggtctgttt ggtacttctt ctcacgctaa ggtggcggtg
781 tgatatgcac aatggctaaa aagcagggag agctggaaag aaacaaggac agagacagag
841 gccaagtcaa ccagaccaat tcccagagga agcaaagaaa ccattacaga gactacaagg
901 gggaagggaa ggagagatga attagcttcc cctgtaaacc ttagaaccca gctgttgcca
961 gggcaacggg gcaatacctg tctcttcaga ggagatgaag ttgccagggt aactacatcc
1021 tgtctttctc aaggaccatc ccagaatgtg gcacccacta gccgttacca tagcaactgc
1081 ctctttgccc cacttaatcc catcccgtct gttaaaaggg ccctatagtt ggaggtgggg
1141 gaggtaggaa gagcgatgat cacttgtgga ctaagtttgt tcacatcccc ttctccaacc
1201 ccctcagtac atcaccctgg gagaacaagg tccacttgct tctgggccca cacagtcctg
1261 cagtattgtg tatataaggc cagggcaacg gaggagcagg ttttgaagtg aaaggcaggc
1321 aggtgttggg gaggcagtta ccggggcaac gggaacaggg cgtttcggag gtggttgcca
1381 tggggacctg gatgctgacg aaggctcgcg aggctgtgag cagccacagt gccctgctca
1441 gaagccccaa gctcgtcaat caagctggtt ctccatttgc actcaggagc acgggcaggc
1501 gagtggcccc tagttctggg ggcagcgggg gatcctaagc caccatggct gacgtggaaa
1561 ccgagaccgg catgattgca cagtggattg tctttgctat tatggctgct gctgctattg
1621 cttttggagt ggctgtgcac tttcggcctt cagagctgaa gagcgcatac tatatcaaca
1681 ttgccatctg cactatcgcc gctaccgctt actatgcaat ggccgtgaac taccaggacc
1741 tgacaatgaa tggtgaaagg caggtggtct acgcaagata tatttcctgg gtgctgacca
1801 caccactgct cctgctcgat ctcatcgtca tgaccaagat gggcggagtg atgatttctt
1861 gggtcatcgg cgcagacatt ttcatgatcg tgtttggtat tctgggcgcc ttcgaggatg
1921 aacacaagtt caaatgggtg tactttatcg ctggatgtgt gatgcaggca gtcctgacat
1981 acgggatgta taacgccact tggaaagacg atctgaagaa aagccccgag taccatagct
2041 cctatgtcag tctgctcgtc ttcctgtcaa tcctctgggt gttttatcct gtcgtgtggg
2101 ctttcgggtc tggtagtggc gtgctgtccg tcgacaatga ggccattctc atgggaatcc
2161 tggatgtgct cgctaagcca ctgtttggaa tggggtgcct cattgcccat gagactatct
2221 tcaagtccta cccagcgaca catgagttac acatctttgg ctccatcaac ggtgtggact
2281 ttgacatggt gggtcagggc accggcaatc caaatgatgg ttatgaggag ttaaacctga
2341 agtccaccaa gggtgacctc cagttctccc cctggattct ggtccctcat atcgggtatg
2401 gcttccatca gtacctgccc taccctgacg ggatgtcgcc tttccaggcc gccatggtag
2461 atggctccgg ctaccaagtc catcgcacaa tgcagtttga agatggtgcc tcccttactg
2521 ttaactaccg ctacacctac gagggaagcc acatcaaagg agaggcccag gtgaagggga
2581 ctggtttccc tgctgacggt cctgtgatga ccaactcgct gaccgctgcg gactggtgca
2641 ggtcgaagaa gacttacccc aacgacaaaa ccatcatcag tacctttaag tggagttaca
2701 ccacgggaaa tggcaagcgc taccggagca ctgcgcggac cacctacacc tttgccaagc
2761 caatggcggc taactatctg aagaaccagc cgatgtacgt gttccgtaag acggagctca
2821 agcactccaa gaccgagctc aacttcaagg agtggcaaaa ggcctttacc gatgtgatgg
2881 gcatggacga gctgtataag aagagcagga tcaccagcga gggcgagtac atccccctgg
2941 accagatcga catcaacgtg ttctgctacg agaacgaggt gcagagccag cctatcctga
3001 acaccaagga gatggcccct cagagcaagc ctcctgagga gctggagatg agcagcatgc
3061 ctagccctgt ggcccctctg cctgccagga ccgagggcgt gatcgacatg aggagcatga
3121 gcagcatcga cagcttcatc agctgcgcca ccgacttccc tgaggccacc aggttctaag
3181 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
3241 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
3301 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
3361 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
3421 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
3481 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
3541 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
3601 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
3661 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
3721 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
3781 ccgcctcccc gcatcgatac cgtcgacccg ggcggccgct tcgagcagac atgataagat
3841 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
3901 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
3961 acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa
4021 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tcttcctaga gcatggctac
4081 gtagataagt agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg
4141 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
4201 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cattaatgaa
4261 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
4321 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
4381 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
4441 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
4501 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
4561 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
4621 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
4681 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
4741 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
4801 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
4861 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
4921 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
4981 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
5041 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
5101 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
5161 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
5221 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
5281 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
5341 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
5401 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
5461 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
5521 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
5581 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
5641 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
5701 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
5761 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
5821 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
5881 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
5941 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
6001 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
6061 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
6121 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
6181 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
6241 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
6301 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
6361 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
6421 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
6481 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
6541 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
6601 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
6661 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg
6721 ggaagg
//
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