| 质粒名称: | pCRISPRia-v2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 84832 |
| 存储实验室: | Jonathan Weissman |
|
载体骨架基本信息 |
|
| 载体名称: | pSICO derivative |
| 载体出品公司: | Addgene |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8200 bp |
| 完整质粒大小: | 8888bp |
| 载体修饰: | 人细胞色素c氧化酶亚基VIII（human cytochrome C oxidase subunit VIII） |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | sgRNA |
| 物种来源: | -- |
| 插入大小: | 113 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pCRISPRia-v2 was a gift from Jonathan Weissman (Addgene plasmid # 84832 ; http://n2t.net/addgene:84832 ; RRID:Addgene_84832) |
| 参考文献部分: | Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation. Horlbeck MA, Gilbert LA, Villalta JE, Adamson B, Pak RA, Chen Y, Fields AP, Park CY, Corn JE, Kampmann M, Weissman JS. Elife. 2016 Sep 23;5. pii: e19760. doi: 10.7554/eLife.19760. 10.7554/eLife.19760 PubMed 27661255 |
买家导航
pCRISPRia-v2载体质粒基本信息
pCRISPRia-v2质粒图谱载体图谱和pCRISPRia-v2载体序列质粒序列多克隆位点信息
pCRISPRia-v2质粒载体简介
pCRISPRia-v2质粒序列载体序列
LOCUS pCRISPRia-v2 8893 bp ds-DNA circular SYN 22-APR-2019
DEFINITION CRISPRi/a V2 library parental plasmid.
ACCESSION .
VERSION .
KEYWORDS pCRISPRia-v2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8893)
AUTHORS Horlbeck MA, Gilbert LA, Villalta JE, Adamson B, Pak RA, Chen Y,
Fields AP, Park CY, Corn JE, Kampmann M, Weissman JS
TITLE Compact and highly active next-generation libraries for
CRISPR-mediated gene repression and activation.
JOURNAL Elife. 2016 Sep 23;5. pii: e19760. doi: 10.7554/eLife.19760.
PUBMED 27661255
REFERENCE 2 (bases 1 to 8893)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 22, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8893
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(47..66)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 772..792
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 835..1015
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1684..1917
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2444..2561
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 2822..2855
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
primer_bind 2859..2879
/label=mU6-F
/note="Mouse U6 promoter, forward primer"
promoter 3132..3343
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
intron 3344..4282
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 4239..4259
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 4303..4899
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(4303..4322)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 4799..4819
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
CDS 4909..4962
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 4975..5673
/codon_start=1
/product="monomeric blue fluorescent protein"
/label=TagBFP
/note="mammalian codon-optimized"
/translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
LPSKLGHKLN"
protein_bind 5693..5726
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
misc_feature 5782..6370
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5835..5855)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(6253..6264)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(6373..6389)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6374..6390)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 6899..7079
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
rep_origin complement(7141..7729)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7221..7240)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7900..8760)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8523..8542
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8761..8865)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
2581 gcagagatcc agtttggtta gtaccgggcc cgccctgcag gtctagagat ccgacgcgcc
2641 atctctaggc ccgcgccggc cccctcgcac ggacttgtgg gagaagctcg gctactcccc
2701 tgccccggtt aatttgcata taatatttcc tagtaactat agaggcttaa tgtgcgataa
2761 aagacagata atctgttctt tttaatacta gctacatttt acatgatagg cttggatttc
2821 tataacttcg tatagcatac attatacgaa gttataaaca gcacaaaagg aaactcaccc
2881 taactgtaaa gtaattgtgt gttttgagac tataagtatc ccttggagaa ccaccttgtt
2941 ggaccaggat gggcaccacc cgtttaagag ctaagctgga aacagcatag caagtttaaa
3001 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttctcgag
3061 tactaggatc cattaggcgg ccgccctgca gggtggataa ccgtattacc gccatgcatg
3121 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
3181 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
3241 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
3301 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
3361 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
3421 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
3481 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
3541 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
3601 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
3661 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
3721 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
3781 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
3841 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
3901 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
3961 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
4021 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
4081 gcgcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
4141 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
4201 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
4261 aagttttttt cttccatttc aggtgtcgtg agctagccca ccatgaccga gtacaagccc
4321 acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg
4381 ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc
4441 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
4501 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
4561 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
4621 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
4681 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
4741 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
4801 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
4861 ggaccgcgca cctggtgcat gacccgcaag cccggtgccg gatcgggaga gggcagagga
4921 agtctgctaa catgcggtga cgtcgaggag aatcctggcc caccggtcgc caccagcgag
4981 ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgga caaccatcac
5041 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc
5101 aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac tagcttcctc
5161 tacggcagca agaccttcat caaccacacc cagggcatcc ccgacttctt caagcagtcc
5221 ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc
5281 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg
5341 gtgaacttca catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccttc
5401 accgagacgc tgtaccccgc tgacggcggc ctggaaggca gaaacgacat ggccctgaag
5461 ctcgtgggcg ggagccatct gatcgcaaac atcaagacca catatagatc caagaaaccc
5521 gctaagaacc tcaagatgcc tggcgtctac tatgtggact acagactgga aagaatcaag
5581 gaggccaaca acgagaccta cgtcgagcag cacgaggtgg cagtggccag atactgcgac
5641 ctccctagca aactggggca caagcttaat taagaattcg tcgagggacc taataacttc
5701 gtatagcata cattatacga agttatacat gtttaagggt tccggttcca ctaggtacaa
5761 ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
5821 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
5881 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
5941 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
6001 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
6061 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
6121 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
6181 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
6241 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
6301 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
6361 gcctccccgc atcgataccg tcgacctcga tcgagaccta gaaaaacatg gagcaatcac
6421 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
6481 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
6541 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
6601 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg
6661 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa
6721 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt
6781 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
6841 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
6901 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
6961 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
7021 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
7081 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
7141 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
7201 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
7261 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
7321 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
7381 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
7441 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
7501 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
7561 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
7621 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
7681 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
7741 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
7801 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
7861 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
7921 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
7981 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
8041 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
8101 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
8161 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
8221 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
8281 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
8341 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
8401 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
8461 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
8521 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
8581 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
8641 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
8701 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
8761 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
8821 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
8881 agtgccacct gac
//
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pCRISPRia-v2质粒载体应用举例
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