pAAVLINKv1-EF1alpha-Cre-3'-SCN1A-FLAG

LOCUS       pAAVLINKv1-EF1alpha-Cre-3'-SCN1A-FLAG            7440 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 15, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        146..687
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        175..386
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             399..667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    493..498
                     /label=SDS
     CDS             701..1732
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1733..1773
                     /label=Splicing Donor
     protein_bind    1778..1811
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1812..1903
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1904..4159
                     /codon_start=1
                     /label=3' SCN1A
                     /translation="VFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTAN
                     ALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIF
                     SIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGF
                     GYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFI
                     GVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFV
                     TRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRH
                     YYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGI
                     RTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMI
                     CLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISF
                     LVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAA
                     ALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEER
                     FMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGA
                     NLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK"
     misc_feature    3154..3159
                     /label=SDS
     CDS             4160..4225
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      4229..4660
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4373..4378
                     /label=SDS
     repeat_region   4703..4843
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4703..4832
                     /label=AAV2 ITR
     repeat_region   4704..4844
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4918..5373
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5655..5759
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5760..6620
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6791..7379
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
      181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
      241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
      421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
      481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
      541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
      601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
      661 acagatccaa gctgtgaccg gcgcctaaac gtacgccacc atgtccaatt tactgaccgt
      721 acaccaaaat ttgcctgcat taccggtcga tgcaacgagt gatgaggttc gcaagaacct
      781 gatggacatg ttcagggatc gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc
      841 cgtttgccgg tcgtgggcgg catggtgcaa gttgaataac cggaaatggt ttcccgcaga
      901 acctgaagat gttcgcgatt atcttctata tcttcaggcg cgcggtctgg cagtaaaaac
      961 catccagcaa catttgggcc agctaaacat gcttcatcgt cggtccgggc tgccacgacc
     1021 aagtgacagc aatgctgttt cactggttat gcggcggatc cgaaaagaaa acgttgatgc
     1081 cggtgaacgt gcaaaacagg ctctagcgtt cgaacgcact gatttcgacc aggttcgttc
     1141 actcatggaa aatagcgatc gctgccagga tatacgtaat ctggcatttc tggggattgc
     1201 ttataacacc ctgttacgta tagccgaaat tgccaggatc agggttaaag atatctcacg
     1261 tactgacggt gggagaatgt taatccatat tggcagaacg aaaacgctgg ttagcaccgc
     1321 aggtgtagag aaggcactta gcctgggggt aactaaactg gtcgagcgat ggatttccgt
     1381 ctctggtgta gctgatgatc cgaataacta cctgttttgc cgggtcagaa aaaatggtgt
     1441 tgccgcgcca tctgccacca gccagctatc aactcgcgcc ctggaaggga tttttgaagc
     1501 aactcatcga ttgatttacg gcgctaagga tgactctggt cagagatacc tggcctggtc
     1561 tggacacagt gcccgtgtcg gagccgcgcg agatatggcc cgcgctggag tttcaatacc
     1621 ggagatcatg caagctggtg gctggaccaa tgtaaatatt gtaatgaact atatccgtaa
     1681 cctggatagt gaaacagggg caatggtgcg cctgctggaa gatggcgatt aggtaagtat
     1741 caaggttaca agacaggttt aaggagacca atagcttata acttcgtata ggatacttta
     1801 tagcaattta tgaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca
     1861 cctattggtc ttactgacat ccactttgcc tttctctcca caggttttca cttacatttt
     1921 cattctggaa atgcttctaa aatgggtggc atatggctat caaacatatt tcaccaatgc
     1981 ctggtgttgg ctggacttct taattgttga tgtttcattg gtcagtttaa cagcaaatgc
     2041 cttgggttac tcagaacttg gagccatcaa atctctcagg acactaagag ctctgagacc
     2101 tctaagagcc ttatctcgat ttgaagggat gagggtggtt gtgaatgccc ttttaggagc
     2161 aattccatcc atcatgaatg tgcttctggt ttgtcttata ttctggctaa ttttcagcat
     2221 catgggcgta aatttgtttg ctggcaaatt ctaccactgt attaacacca caactggtga
     2281 caggtttgac atcgaagacg tgaataatca tactgattgc ctaaaactaa tagaaagaaa
     2341 tgagactgct cgatggaaaa atgtgaaagt aaactttgat aatgtaggat ttgggtatct
     2401 ctctttgctt caagttgcca cattcaaagg atggatggat ataatgtatg cagcagttga
     2461 ttccagaaat gtggaactcc agcctaagta tgaagaaagt ctgtacatgt atctttactt
     2521 tgttattttc atcatctttg ggtccttctt caccttgaac ctgtttattg gtgtcatcat
     2581 agataatttc aaccagcaga aaaagaagtt tggaggtcaa gacatcttta tgacagaaga
     2641 acagaagaaa tactataatg caatgaaaaa attaggatcg aaaaaaccgc aaaagcctat
     2701 acctcgacca ggaaacaaat ttcaaggaat ggtctttgac ttcgtaacca gacaagtttt
     2761 tgacataagc atcatgattc tcatctgtct taacatggtc acaatgatgg tggaaacaga
     2821 tgaccagagt gaatatgtga ctaccatttt gtcacgcatc aatctggtgt tcattgtgct
     2881 atttactgga gagtgtgtac tgaaactcat ctctctacgc cattattatt ttaccattgg
     2941 atggaatatt tttgattttg tggttgtcat tctctccatt gtaggtatgt ttcttgccga
     3001 gctgatagaa aagtatttcg tgtcccctac cctgttccga gtgatccgtc ttgctaggat
     3061 tggccgaatc ctacgtctga tcaaaggagc aaaggggatc cgcacgctgc tctttgcttt
     3121 gatgatgtcc cttcctgcgt tgtttaacat cggcctccta ctcttcctag tcatgttcat
     3181 ctacgccatc tttgggatgt ccaactttgc ctatgttaag agggaagttg ggatcgatga
     3241 catgttcaac tttgagacct ttggcaacag catgatctgc ctattccaaa ttacaacctc
     3301 tgctggctgg gatggattgc tagcacccat tctcaacagt aagccacccg actgtgaccc
     3361 taataaagtt aaccctggaa gctcagttaa gggagactgt gggaacccat ctgttggaat
     3421 tttctttttt gtcagttaca tcatcatatc cttcctggtt gtggtgaaca tgtacatcgc
     3481 ggtcatcctg gagaacttca gtgttgctac tgaagaaagt gcagagcctc tgagtgagga
     3541 tgactttgag atgttctatg aggtttggga gaagtttgat cccgatgcaa ctcagttcat
     3601 ggaatttgaa aaattatctc agtttgcagc tgcgcttgaa ccgcctctca atctgccaca
     3661 accaaacaaa ctccagctca ttgccatgga tttgcccatg gtgagtggtg accggatcca
     3721 ctgtcttgat atcttatttg cttttacaaa gcgggttcta ggagagagtg gagagatgga
     3781 tgctctacga atacagatgg aagagcgatt catggcttcc aatccttcca aggtctccta
     3841 tcagccaatc actactactt taaaacgaaa acaagaggaa gtatctgctg tcattattca
     3901 gcgtgcttac agacgccacc ttttaaagcg aactgtaaaa caagcttcct ttacgtacaa
     3961 taaaaacaaa atcaaaggtg gggctaatct tcttataaaa gaagacatga taattgacag
     4021 aataaatgaa aactctatta cagaaaaaac tgatctgacc atgtccactg cagcttgtcc
     4081 accttcctat gaccgggtga caaagccaat tgtggaaaaa catgagcaag aaggcaaaga
     4141 tgaaaaagcc aaagggaaag actacaaaga ccatgacggt gattataaag atcatgacat
     4201 cgactacaag gatgacgatg acaagtaact cgagataatc aacctctgga ttacaaaatt
     4261 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     4321 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     4381 tataaatcct ggttagttct tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     4441 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgtttatttg tgaaatttgt
     4501 gatgctattg ctttatttgt aaccatctag ctttatttgt gaaatttgtg atgctattgc
     4561 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     4621 tatgtttcag gttcaggggg agatgtggga ggttttttaa agatctggag accggtgtgg
     4681 cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg
     4741 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
     4801 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat
     4861 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
     4921 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4981 cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     5041 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     5101 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
     5161 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     5221 tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt gatttataag
     5281 ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     5341 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
     5401 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     5461 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     5521 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     5581 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     5641 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     5701 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     5761 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     5821 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     5881 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     5941 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6001 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6061 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6121 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6181 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6241 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6301 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6361 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     6421 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     6481 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     6541 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     6601 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     6661 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     6721 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6781 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6841 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6901 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     6961 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7021 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7081 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7141 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7201 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7261 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7321 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7381 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//