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pcDNA3.1+N-6HIS载体质粒基本信息
pcDNA3.1+N-6HIS质粒图谱载体图谱和pcDNA3.1+N-6HIS载体序列质粒序列多克隆位点信息
pcDNA3.1+N-6HIS质粒载体简介
pcDNA3.1+N-6HIS质粒序列载体序列
LOCUS pcDNA3.1+N-6His 5455 bp DNA circular SYN 27-AUG-2021
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTDATE|-14017131|
COMMENT VNTDBDATE|-14017131|
COMMENT LSOWNER|
COMMENT VNTNAME|pcDNA3.1+N-6His|
COMMENT VNTAUTHORNAME|Biofeng.com|
COMMENT VNTAUTHOREML|order@biofeng.com|
COMMENT VNTOAUTHORNAME|Invitrogen|
COMMENT VNTOAUTHORTEL|(800)-357-3114|
COMMENT VNTOAUTHORFAX|(760)-602-6500|
COMMENT VNTOAUTHOREML|bioinfosales@invitrogen.com|
COMMENT VNTOAUTHORAD1|Invitrogen Corporation|
COMMENT VNTOAUTHORAD2|1620 Faraday Avenue|
COMMENT VNTOAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
FEATURES Location/Qualifiers
source 1..5455
/invitrogen="124"
/vntifkey="98"
CDS complement(4459..5319)
/invitrogen="1020000"
/vntifkey="4"
/label=Amp(R)
polyA_site 1055..1279
/invitrogen="1870000"
/vntifkey="26"
/label=BGH\pA
primer complement(1049..1066)
/invitrogen="1910000"
/vntifkey="27"
/label=BGH\reverse\primer
promoter complement(5320..5418)
/invitrogen="2330000"
/vntifkey="30"
/label=bla\promoter
primer 769..789
/invitrogen="1930000"
/vntifkey="27"
/label=CMV\forward\primer
promoter 232..819
/invitrogen="2180000"
/vntifkey="29"
/label=CMV\promoter
rep_origin 1325..1753
/invitrogen="2500000"
/vntifkey="33"
/label=f1\origin
misc_feature 944..1037
/invitrogen="1630000"
/vntifkey="21"
/label=MCS
CDS 2163..2957
/invitrogen="1260000"
/vntifkey="4"
/label=Neo(R)
rep_origin complement(3644..4314)
/invitrogen="2540000"
/vntifkey="33"
/label=pUC\origin
promoter 1758..2127
/invitrogen="2290000"
/vntifkey="29"
/label=SV40\early\promoter
polyA_signal 3131..3261
/invitrogen="1840000"
/vntifkey="25"
/label=SV40\pA
primer 863..882
/invitrogen="2100000"
/vntifkey="27"
/label=T7\primer
promoter 863..879
/invitrogen="2370000"
/vntifkey="30"
/label=T7\promoter
misc_feature 917..922
/vntifkey="21"
/label=Kozak\sequence
misc_feature 926..943
/vntifkey="21"
/label=His\Tag
BASE COUNT 1259 a 1423 c 1388 g 1385 t
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttgcca ccatgcatca tcaccatcac catggtaccg agctcggatc
961 cactagtcca gtgtggtgga attctgcaga tatccagcac agtggcggcc gctcgagtct
1021 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
1081 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
1141 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
1201 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
1261 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
1321 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
1381 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
1441 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
1501 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
1561 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
1621 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
1681 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
1741 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
1801 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
1861 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
1921 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
1981 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
2041 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
2101 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
2161 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
2221 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
2281 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
2341 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
2401 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
2461 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
2521 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
2581 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
2641 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
2701 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
2761 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
2821 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
2881 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
2941 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
3001 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
3061 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
3121 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
3181 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
3241 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
3301 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
3361 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
3421 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
3481 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
3541 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
3601 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
3661 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
3721 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
3781 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
3841 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
3901 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
3961 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
4021 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
4081 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
4141 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
4201 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca
4261 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
4321 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
4381 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
4441 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
4501 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
4561 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
4621 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
4681 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
4741 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
4801 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
4861 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
4921 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
4981 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
5041 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
5101 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
5161 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
5221 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
5281 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
5341 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
5401 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
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