pLVX-TetOn-TcdB3GTD-EGFP

LOCUS       pLVX-TetOn-TcdB3GTD-EGFP              11616 bp DNA     circular SYN 06-DEC-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11616)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..11616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          6707..8335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    216..350
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        398..502
                     /gene="bla"
                     /label=AmpR promoter
     CDS             503..1363
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1534..2119
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2407..2428
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2443..2473
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2481..2497
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2505..2521
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             2650..3283
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3491..4079
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3597..3608
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(4093..4692)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        complement(4701..5030)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4715..4850
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(5041..5787)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(5806..6316)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     promoter        6333..6700
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    6341..6359
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6377..6395
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6413..6431
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6449..6467
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6485..6503
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6521..6539
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6557..6575
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     6690..6744
                     /label=Tet-TcdB3-F
     misc_feature    6707..8335
                     /label=TcdB3GTD
                     /note="TcdB"
     CDS             6707..8335
                     /codon_start=1
                     /label=Feature 5
                     /translation="MSLVNRKQLEKMANVRFRVQEDEYVAILDALEEYHNMSENTVVEK
                     YLKLKDINSLTDTYIDTYKKSGRNKALKKFKEYLVIEILELKNSNLTPVEKNLHFIWIG
                     GQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTIIESASNDTLESFRENLND
                     PEFNHTAFFRKRMQIIYDKQQNFINYYKAQKEENPDLIIDDIVKTYLSNEYSKDIDELN
                     AYIEESLNKVTENSGNDVRNFEEFKTGEVFNLYEQELVERWNLAGASDILRVAILKNIG
                     GVYLDVDMLPGIHPDLFKDINKPDSVKTAVDWEEMQLEAIMKHKEYIPEYTSKHFDTLD
                     EEVQSSFESVLASKSDKSEIFLPLGDIEVSPLEVKIAFAKGSIINQALISAKDSYCSDL
                     LIKQIQNRYKILNDTLGPIISQGNDFNTTMNNFGESLGAIANEENISFIAKIGSYLRVG
                     FYPEANTTITLSGPTIYAGAYKDLLTFKEMSIDTSILSSELRNFEFPKVNISQATEQEK
                     NSLWQFNEERAKIQFEEYKKNYFEGA"
     misc_feature    7109..7131
                     /label=B1/3
                     /note="B1/3"
     primer_bind     complement(8303..8353)
                     /label=Tet-TcdB3GTD-R
     CDS             8342..8398
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8399..9115
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    9296..9430
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    9474..9589
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(9852..9896)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10081..10314
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    10811..10936
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             10983..11616
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg
       61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg
      121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt
      181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg
      241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
      301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga
      361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
      721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
      781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
      961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct
     1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc
     2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg
     2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac
     2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag
     2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc
     2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac
     2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag
     2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga
     3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca
     3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta
     3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct
     3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga
     3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt
     3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt
     3361 gtgactggaa aaacccacct cctcctcctc ttgtgcttct agccaggcac aatcagcatt
     3421 ggtagctgct gtattgctac ttgtgattgc tccatgtttt tctaggtctc gactgcagaa
     3481 ttaattccag gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga
     3541 agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc cgctggattg
     3601 agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg
     3661 agcagccatg gaaaggacgt cagcttcccc gacaacacca cggaattgtc agtgcccaac
     3721 agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata
     3781 gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca
     3841 gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac
     3901 aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat gaaagccata
     3961 cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag cgtaaaagga
     4021 gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgattg
     4081 ttccagacgc gttcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg
     4141 cacctcgacg tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg
     4201 cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag
     4261 cacgacggcg ctgcccagac ccttgccctg gtggtcgggc gagacgccga cggtggccag
     4321 gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc
     4381 ggccagccgg gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc
     4441 cgcttcgacg ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca
     4501 caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg
     4561 ctcgatgtgg cggtccgggt cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc
     4621 ggcgagggtg cgtacggccc gggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt
     4681 gtactcggtc atggtcgcgt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac
     4741 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt
     4801 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt
     4861 aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     4921 ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata
     4981 cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac attcgcgcgt
     5041 ttacccgggg agcatgtcaa ggtcaaaatc gtcaagagcg tcagcaggca gcatatcaag
     5101 gtcaaagtcg tcaagggcat cggctgggag catgtctaag tcaaaatcgt caagggcgtc
     5161 ggtcggcccg ccgctttcgc actttagctg tttctccagg ccacatatga ttagttccag
     5221 gccgaaaagg aaggcaggtt cggctccctg ccggtcgaac agctcaattg cttgtttcag
     5281 aagtgggggc atagaatcgg tggtaggtgt ctctctttcc tcttttgcta cttgatgctc
     5341 ctgttcctcc aatacgcagc ccagtgtaaa gtggcccacg gcggacagag cgtacagtgc
     5401 gttctccagg gagaagcctt gctgacacag gaacgcgagc tgattttcca gggtttcgta
     5461 ctgtttctct gttgggcggg tgccgagatg cactttagcc ccgtcgcgat gtgagaggag
     5521 agcacagcgg tatgacttgg cgttgttccg cagaaagtct tgccatgact cgccttccag
     5581 ggggcaggag tgggtatgat gcctgtccag catctcgatt ggcagggcat cgagcagggc
     5641 ccgcttgttc ttcacgtgcc agtacagggt aggctgctca actcccagct tttgagcgag
     5701 tttccttgtc gtcaggcctt cgataccgac tccattgagt aattccagag cagagtttat
     5761 gactttgctc ttgtccagtc tagacatggt aattcgatga tccccctggg gagagaggtc
     5821 ggtgattcgg tcaacgaggg agccgactgc cgacgtgcgc tccggaggct tgcagaatgc
     5881 ggaacaccgc gcgggcagga acagggccca cactaccgcc ccacaccccg cctcccgcac
     5941 cgccccttcc cggccgctgc tctcggcgcg ccctgctgag cagccgctat tggccacagc
     6001 ccatcgcggt cggcgcgctg ccattgctcc ctggcgctgt ccgtctgcga gggtactagt
     6061 gagacgtgcg gcttccgttt gtcacgtccg gcacgccgcg aaccgcaagg aaccttcccg
     6121 acttaggggc ggagcaggaa gcgtcgccgg ggggcccaca agggtagcgg cgaagatccg
     6181 ggtgacgctg cgaacggacg tgaagaatgt gcgagaccca gggtcggcgc cgctgcgttt
     6241 cccggaacca cgcccagagc agccgcgtcc ctgcgcaaac ccagggctgc cttggaaaag
     6301 gcgcaacccc aaccccgtgg aattatcacc tcgagtttac tccctatcag tgatagagaa
     6361 cgtatgaaga gtttactccc tatcagtgat agagaacgta tgcagacttt actccctatc
     6421 agtgatagag aacgtataag gagtttactc cctatcagtg atagagaacg tatgaccagt
     6481 ttactcccta tcagtgatag agaacgtatc tacagtttac tccctatcag tgatagagaa
     6541 cgtatatcca gtttactccc tatcagtgat agagaacgta taagctttgc ttatgtaaac
     6601 cagggcgcct ataaaagagt gctgattttt tgagtaaact tcaattccac aacacttttg
     6661 tcttatacca actttccgta ccacttccta ccctcgtaaa gaattcatga gtttagttaa
     6721 tagaaaacag ttagaaaaaa tggcaaatgt aagatttcgt gttcaggaag atgaatatgt
     6781 agcaatatta gatgcattag aagaatatca taatatgtca gaaaatactg tagttgaaaa
     6841 gtatctaaaa ttaaaagata taaacagttt aacagatact tatatagata catataaaaa
     6901 atctggtcga aataaagcct taaaaaaatt taaagagtac ttagttatag agatattaga
     6961 attaaaaaat agcaatttaa ctccagtcga gaaaaattta cattttatat ggattggagg
     7021 gcaaataaat gatactgcta ttaattatat aaatcaatgg aaagatgtaa atagtgacta
     7081 taatgttaat gttttttatg atagtaatgc atttttgata aacacattga aaaaaactat
     7141 aatagaatca gcatcaaatg atacccttga atcatttaga gaaaatttaa atgatcctga
     7201 atttaaccac actgcatttt tcagaaaacg tatgcaaata atctatgata aacagcaaaa
     7261 tttcataaat tactataaag ctcaaaaaga agaaaatcct gaccttataa ttgatgatat
     7321 tgtaaagaca tatctttcaa acgagtattc aaaggatata gatgaactta atgcttatat
     7381 tgaagagtca ttaaacaaag tcacagaaaa tagtggaaat gatgttagaa actttgaaga
     7441 atttaaaact ggagaagtat tcaatttata tgaacaagag ttagtagaaa gatggaatct
     7501 tgctggtgca tctgatatat taagagtcgc tatattgaaa aatattggtg gagtctatct
     7561 agatgttgat atgttaccag gaatacaccc agatttattt aaagatataa ataagcctga
     7621 ttcagtaaag acagctgtag attgggaaga gatgcagtta gaagccataa tgaaacataa
     7681 agaatatata ccagaatata cttcaaaaca ttttgataca ttggatgaag aagttcaaag
     7741 tagctttgaa tctgttttag cttctaagtc tgataagtca gaaatatttt taccactagg
     7801 agatatagag gtatcacctt tagaagtaaa aattgcattt gccaaaggtt ctattataaa
     7861 tcaagctcta atttctgcaa aagattcata ttgtagtgac ttactaataa aacaaatcca
     7921 aaacagatat aagatactga atgatacttt aggtccaatt attagtcaag gtaatgattt
     7981 taatactaca atgaacaatt ttggtgaaag tttgggagct atagctaatg aagagaatat
     8041 aagttttata gcaaaaatcg gaagttattt aagggttgga ttttatcctg aagctaatac
     8101 tacaattact ttaagtggtc ctacaatata tgcaggagct tataaagatt tattaacatt
     8161 taaagagatg agcatagata cttctatatt gtcatctgag ttaagaaatt ttgaatttcc
     8221 taaggttaat atatctcaag caacagaaca agagaaaaat agtttatggc aatttaatga
     8281 agaaagagct aaaattcaat ttgaagaata caagaaaaat tattttgaag gtgcaaccgg
     8341 tgccaccaac ttcagcctgc tgaagcaggc cggcgacgtg gaggagaacc ccggccccgt
     8401 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     8461 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     8521 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     8581 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     8641 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     8701 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     8761 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     8821 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     8881 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     8941 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     9001 gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     9061 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaggatc
     9121 cagaccacct cccctgcgag ctaagctgga cagccaatga cgggtaagag agtgacattt
     9181 ttcactaacc taagacagga gggccgtcag agctactgcc taatccaaag acgggtaaaa
     9241 gtgataaaaa tgtatcactc caacctaaga caggcgcagc ttccgaggga tttgagatcc
     9301 agacatgata agatacattg atgagtttgg acaaaccaaa actagaatgc agtgaaaaaa
     9361 atgccttatt tgtgaaattt gtgatgctat tgccttattt gtaaccatta taagctgcaa
     9421 taaacaagtt aagtcgataa actggatctc tgctgtccct gtaataaacc cgaaaatttt
     9481 gaatttttgt aatttgtttt tgtagttctt tagtttgtat gtctgttgct attatgtcta
     9541 ctattctttc ccctgcactg taccccccaa tccccccttt tcttttaaag gcgataccgt
     9601 cgagatccgt tcactaatcg aatggatctg tctctgtctc tctctccacc ttcttcttct
     9661 attccttcgg gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa cgataatggt
     9721 gaatatccct gcctaactct attcactata gaaagtacag caaaaactat tcttaaacct
     9781 accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt tgttatgtta
     9841 aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc attcttttct
     9901 tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt gttaatttct
     9961 ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt attactccaa
    10021 ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc ccaaatcccc
    10081 aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg gagctgcttg
    10141 atgccccaga ctgtgagttg caacagatgc tgttgcgcct caatagccct cagcaaattg
    10201 ttctgctgct gcactatacc agacaataat tgtctggcct gtaccgtcag cgtcattgac
    10261 gctgcgccca tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc tcctattccc
    10321 actgctcttt tttctctctg caccactctt ctctttgcct tggtgggtgc tactcctaat
    10381 ggttcaattt ttactacttt atatttatat aattcacttc tccaattgtc cctcatatct
    10441 cctcctccag gtctgaagat cagcggccgg ccgcttgctg tgcggtggtc ttacttttgt
    10501 tttgctcttc ctctatcttg tctaaagctt ccttggtgtc ttttatctct atcctttgat
    10561 gcacacaata gagggttgct actgtattat ataatgatct aagttcttct gatcctgtct
    10621 gaagggatgg ttgtagctgt cccagtattt gtctacagcc ttctgatgtt tctaacaggc
    10681 caggattaac tgcgaatcgt tctagctccc tgcttgccca tactatatgt tttaatttat
    10741 attttttctt tccccctggc cttaaccgaa ttttttccca tcgcgatcta attctccccc
    10801 gcttaatact gacgctctcg cacccatctc tctccttcta gcctccgcta gtcaaaattt
    10861 ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag
    10921 tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc
    10981 actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc
    11041 acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag
    11101 cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga
    11161 cccagtacag gcaaaaagca gctgcttata tgcaggatct gagggctcgc cactccccag
    11221 tcccgcccag gccacgcctc cctggaaagt ccccagcgga aagtcccttg tagcaagctc
    11281 gatatcagca gttcttgaag tactccggat gcagctctcg ggccacgtga tgaaatgcta
    11341 ggcggctgtc aaacctccac tctaacactt ctctctccgg gtcatccatc ccatgcaggc
    11401 tcacagggtg taacaagctg gtgttctctc ctttattggc ctcttctacc ttatctggct
    11461 caactggtac tagcttgtag caccatccaa aggtcagtgg atatctgacc cctggccctg
    11521 gtgtgtagtt ctgctaatca gggaagtagc cttgtgtgtg gtagatccac agatcaagga
    11581 tatcttgtct tctttgggag tgaattagcc cttcca
//