| 质粒名称: | Ai9 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 22799 |
| 存储实验室: | Hongkui Zeng |
|
载体骨架基本信息 |
|
| 载体名称: | Rosa26 Targeting Vector |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 9862 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | CAG-Floxed tdTomato |
| 物种来源: | -- |
| 插入大小: | 7057 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | Ai9 was a gift from Hongkui Zeng (Addgene plasmid # 22799 ; http://n2t.net/addgene:22799 ; RRID:Addgene_22799) |
| 参考文献部分: | A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, Ng LL, Palmiter RD, Hawrylycz MJ, Jones AR, Lein ES, Zeng H. Nat Neurosci. 2010 Jan . 13(1):133-40. 10.1038/nn.2467 PubMed 20023653 |
买家导航
Ai9载体质粒基本信息
Ai9质粒图谱载体图谱和Ai9载体序列质粒序列多克隆位点信息
Ai9质粒载体简介
Ai9质粒序列载体序列
LOCUS Ai9 17021 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Ai9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 17021)
AUTHORS Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, Ng LL,
Palmiter RD, Hawrylycz MJ, Jones AR, Lein ES, Zeng H
TITLE A robust and high-throughput Cre reporting and characterization
system for the whole mouse brain.
JOURNAL Nat Neurosci. 2010 Jan . 13(1):133-40.
PUBMED 20023653
REFERENCE 2 (bases 1 to 17021)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..17021
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 3..1085
/label=Rosa26 left arm
/note="left homology arm from intron 1 of the mouse Rosa26
gene (Soriano, 1999)"
enhancer 1181..1484
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1486..1762
/label=chicken beta-actin promoter
intron 1769..2785
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 2709..2731
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 2793..2812
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
protein_bind 2861..2894
/label=FRT (minimal)
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
protein_bind complement(2918..2951)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
polyA_signal 3118..3252
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
polyA_signal 3372..3506
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
polyA_signal 3626..3760
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
protein_bind complement(3789..3822)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
regulatory 3897..3906
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3903..5333
/codon_start=1
/product="tandem dimeric (pseudo-monomeric) derivative of
DsRed (Shaner et al., 2004)"
/label=tdTomato
/note="mammalian codon-optimized"
/translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
RSEGRHHLFLYGMDELYK"
misc_feature 5399..5987
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5452..5472)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5870..5881)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(5990..6006)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5991..6007)
/label=pBluescriptKS
/note="For pBluescript vector"
polyA_signal 6014..6221
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
protein_bind 6229..6262
/label=attB
/bound_moiety="phage phi-C31 integrase"
/note="minimal attB site for the phi-C31 integrase (Groth
et al., 2000)"
promoter 6284..6783
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
protein_bind 6803..6836
/label=FRT (minimal)
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
regulatory 6837..6846
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 6843..7646
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6906..6925)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 7516..7535
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 7656..8117
/label=PGK poly(A) signal
/note="mouse phosphoglycerate kinase 1 polyadenylation
signal"
misc_feature 8183..12523
/label=Rosa26 right arm
/note="right homology arm from intron 1 of the mouse Rosa26
gene (Soriano, 1999)"
promoter 12573..13072
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
CDS 13099..13671
/codon_start=1
/gene="Corynebacterium diphtheriae tox"
/product="diphtheria toxin fragment A"
/label=DTA
/translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
NFETRGKRGQDAMYEYMAQACAGNRVRR"
intron 13748..13813
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
polyA_signal 13887..14111
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(14173..14193)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(14173..14191)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(14212..14228)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(14212..14228)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(14225..14247)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 14236..14252
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(14260..14290)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 14305..14326
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(14443..14460)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(14614..15202)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(14694..14713)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(15373..16233)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 15996..16015
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(16234..16338)
/gene="bla"
/label=AmpR promoter
rep_origin complement(16364..16819)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(16501..16522)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 16713..16732
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 16946..16968
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 16960..16977
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 16961..16977
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 16987..17006
/label=T7
/note="T7 promoter, forward primer"
promoter 16987..17005
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 ccgcggcagg ccctccgagc gtggtggagc cgttctgtga gacagccggg tacgagtcgt
61 gacgctggaa ggggcaagcg ggtggtgggc aggaatgcgg tccgccctgc agcaaccgga
121 gggggaggga gaagggagcg gaaaagtctc caccggacgc ggccatggct cggggggggg
181 ggggcagcgg aggagcgctt ccggccgacg tctcgtcgct gattggcttc ttttcctccc
241 gccgtgtgtg aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg
301 gcagccggag tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta
361 ggtggggtga ggcgagctgg acgtgcgggc gcggtcggcc tctggcgggg cggggggggg
421 gggggggggt cagcgaaagt agctcgcgcg cgagcggccg cccaccctcc ccttcctctg
481 ggggagtcgt tttacccgcc gccggccggg cctcgtcgtc tgattggctc tcggggccca
541 gaaaactggc ccttgccatt ggctcgtgtt cgtgcaagtt gagtccatcc gccggccagc
601 gggggcggcg aggaggcgct cccaggttcc ggccctcccc tcggccccgc gccgcagagt
661 ctggccgcgc gcccctgcgc aacgtggcag gaagcgcgcg ctgggggcgg ggacgggcag
721 tagggctgag cggctgcggg gcgggtgcaa gcacgtttcc gacttgagtt gcctcaagag
781 gggcgtgctg agccagacct ccatcgcgca ctccggggag tggagggaag gagcgagggc
841 tcagttgggc tgttttggag gcaggaagca cttgctctcc caaagtcgct ctgagttgtt
901 atcagtaagg gagctgcagt ggagtaggcg gggagaaggc cgcacccttc tccggagggg
961 ggaggggagt gttgcaatac ctttctggga gttctctgct gcctcctggc ttctgaggac
1021 cgccctgggc ctgggagaat cccttccccc tcttccctcg tgatctgcaa ctccagtctt
1081 tctagcctta attaaccgtt taaacaattc tgcaggaatc tagttattaa tagtaatcaa
1141 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
1201 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
1261 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
1321 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
1381 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
1441 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
1501 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
1561 ttttttaatt attttgtgca gcgatggggg cggggggggg ggggggsgcg cgccaggcgg
1621 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca
1681 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa
1741 aaagcgaagc gcgcggcggg cgcgggcggg agtcgctgcg cgctgccttc gccccgtgcc
1801 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
1861 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg
1921 gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg
1981 cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg
2041 ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg
2101 cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga
2161 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
2221 gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc
2281 ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg
2341 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga
2401 ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
2461 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga
2521 gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg
2581 cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct
2641 tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
2701 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt
2761 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca
2821 tcattttggc aaagaattga tttgataccg cgggccctaa gaagttccta ttctctagaa
2881 agtataggaa cttcgtcgac atttaaatca tttaaatata acttcgtata atgtatgcta
2941 tacgaagtta ttcgcgatga ataaatgaaa gcttgcagat ctgcgactct agaggatctg
3001 cgactctaga ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
3061 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
3121 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
3181 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
3241 catgtctgga tctgcgactc tagaggatca taatcagcca taccacattt gtagaggttt
3301 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
3361 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
3421 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
3481 tcaatgtatc ttatcatgtc tggatctgcg actctagagg atcataatca gccataccac
3541 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
3601 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
3661 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
3721 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cccatcaagc tgatccggaa
3781 cccttaatat aacttcgtat aatgtatgct atacgaagtt attaggtccc tcgacctgca
3841 gcccaagcta gatcgaattc ggccggcctt gtacgcgtta agtgcaacac gatcccgcca
3901 ccatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg
3961 agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
4021 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
4081 acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca
4141 tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
4201 tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga
4261 tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga
4321 agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg
4381 gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga
4441 ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc
4501 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg
4561 gccgccacca cctgttcctg gggcatggca ccggcagcac cggcagcggc agctccggca
4621 ccgcctcctc cgaggacaac aacatggccg tcatcaaaga gttcatgcgc ttcaaggtgc
4681 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
4741 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
4801 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg
4861 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
4921 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca
4981 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc
5041 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
5101 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt
5161 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca
5221 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
5281 ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag taagaattgt
5341 gttgcactta acgcgtacaa ggccggccct gcaggaattc gatatcaagc ttatcgataa
5401 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
5461 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
5521 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
5581 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
5641 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
5701 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
5761 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
5821 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
5881 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
5941 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
6001 acctcgacct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
6061 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
6121 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
6181 aagggggagg attgggaaga caatggcagg catgctgggg aactagtggt gccagggcgt
6241 gcccttgggc tccccgggcg cggcggccgc atcgaattct accgggtagg ggaggcgctt
6301 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
6361 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
6421 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
6481 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
6541 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
6601 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
6661 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
6721 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
6781 tcgacctgca atcgccgcta gcgaagttcc tattctctag aaagtatagg aacttcgcca
6841 ccatgggatc ggccattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
6901 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
6961 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
7021 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
7081 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
7141 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
7201 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
7261 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
7321 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
7381 gcatgcccga cggcgatgat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
7441 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
7501 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
7561 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
7621 atcgccttct tgacgagttc ttctgagggg atccgctgta agtctgcaga aattgatgat
7681 ctattaaaca ataaagatgt ccactaaaat ggaagttttt cctgtcatac tttgttaaga
7741 agggtgagaa cagagtacct acattttgaa tggaaggatt ggagctacgg gggtgggggt
7801 ggggtgggat tagataaatg cctgctcttt actgaaggct ctttactatt gctttatgat
7861 aatgtttcat agttggatat cataatttaa acaagcaaaa ccaaattaag ggccagctca
7921 ttcctcccac tcatgatcta tagatctata gatctctcgt gggatcattg tttttctctt
7981 gattcccact ttgtggttct aagtactgtg gtttccaaat gtgtcagttt catagcctga
8041 agaacgagat cagcagcctc tgttccacat acacttcatt ctcagtattg ttttgccaag
8101 ttctaattcc atcagaaagc ttgcagaaga tctccccaac tggggtaacc tttgagttct
8161 ctcagttggg ggggcgcgcc ggctagaaga tgggcgggag tcttctgggc aggcttaaag
8221 gctaacctgg tgtgtgggcg ttgtcctgca ggggaattga acaggtgtaa aattggaggg
8281 acaagacttc ccacagattt tcggttttgt cgggaagttt tttaataggg gcaaataagg
8341 aaaatgggag gataggtagt catctggggt tttatgcagc aaaactacag gttattattg
8401 cttgtgatcc gcctcggagt attttccatc gaggtagatt aaagacatgc tcacccgagt
8461 tttatactct cctgcttgag atccttacta cagtatgaaa ttacagtgtc gcgagttaga
8521 ctatgtaagc agaattttaa tcatttttaa agagcccagt acttcatatc catttctccc
8581 gctccttctg cagccttatc aaaaggtatt ttagaacact cattttagcc ccattttcat
8641 ttattatact ggcttatcca acccctagac agagcattgg cattttccct ttcctgatct
8701 tagaagtctg atgactcatg aaaccagaca gattagttac atacaccaca aatcgaggct
8761 gtagctgggg cctcaacact gcagttcttt tataactcct tagtacactt tttgttgatc
8821 ctttgccttg atccttaatt ttcagtgtct atcacctctc ccgtcaggtg gtgttccaca
8881 tttgggccta ttctcagtcc agggagtttt acaacaatag atgtattgag aatccaacct
8941 aaagcttaac tttccactcc catgaatgcc tctctccttt ttctccattt ataaactgag
9001 ctattaacca ttaatggttt ccaggtggat gtctcctccc ccaatattac ctgatgtatc
9061 ttacatattg ccaggctgat attttaagac attaaaaggt atatttcatt attgagccac
9121 atggtattga ttactgctta ctaaaatttt gtcattgtac acatctgtaa aaggtggttc
9181 cttttggaat gcaaagttca ggtgtttgtt gtctttcctg acctaaggtc ttgtgagctt
9241 gtattttttc tatttaagca gtgctttctc ttggactggc ttgactcatg gcattctaca
9301 cgttattgct ggtctaaatg tgattttgcc aagcttcttc aggacctata attttgcttg
9361 acttgtagcc aaacacaagt aaaatgatta agcaacaaat gtatttgtga agcttggttt
9421 ttaggttgtt gtgttgtgtg tgcttgtgct ctataataat actatccagg ggctggagag
9481 gtggctcgga gttcaagagc acagactgct cttccagaag tcctgagttc aattcccagc
9541 aaccacatgg tggctcacaa ccatctgtaa tgggatctga tgccctcttc tggtgtgtct
9601 gaagaccaca agtgtattca cattaaataa ataaatcctc cttcttcttc tttttttttt
9661 ttttaaagag aatactgtct ccagtagaat ttactgaagt aatgaaatac tttgtgtttg
9721 ttccaatatg gtagccaata atcaaattac tctttaagca ctggaaatgt taccaaggaa
9781 ctaattttta tttgaagtgt aactgtggac agaggagcca taactgcaga cttgtgggat
9841 acagaagacc aatgcagact ttaatgtctt ttctcttaca ctaagcaata aagaaataaa
9901 aattgaactt ctagtatcct atttgtttaa actgctagct ttacttaact tttgtgcttc
9961 atctatacaa agctgaaagc taagtctgca gccattacta aacatgaaag caagtaatga
10021 taattttgga tttcaaaaat gtagggccag agtttagcca gccagtggtg gtgcttgcct
10081 ttatgccttt aatcccagca ctctggaggc agagacaggc agatctctga gtttgagccc
10141 agcctggtct acacatcaag ttctatctag gatagccagg aatacacaca gaaaccctgt
10201 tggggagggg ggctctgaga tttcataaaa ttataattga agcattccct aatgagccac
10261 tatggatgtg gctaaatccg tctacctttc tgatgagatt tgggtattat tttttctgtc
10321 tctgctgttg gttgggtctt ttgacactgt gggctttctt taaagcctcc ttcctgccat
10381 gtggtctctt gtttgctact aacttcccat ggcttaaatg gcatggcttt ttgccttcta
10441 agggcagctg ctgagatttg cagcctgatt tccagggtgg ggttgggaaa tctttcaaac
10501 actaaaattg tcctttaatt ttttttttaa aaaatgggtt atataataaa cctcataaaa
10561 tagttatgag gagtgaggtg gactaatatt aaatgagtcc ctcccctata aaagagctat
10621 taaggctttt tgtcttatac ttaacttttt ttttaaatgt ggtatcttta gaaccaaggg
10681 tcttagagtt ttagtataca gaaactgttg catcgcttaa tcagattttc tagtttcaaa
10741 tccagagaat ccaaattctt cacagccaaa gtcaaattaa gaatttctga cttttaatgt
10801 taatttgctt actgtgaata taaaaatgat agcttttcct gaggcagggt ctcactatgt
10861 atctctgcct gatctgcaac aagatatgta gactaaagtt ctgcctgctt ttgtctcctg
10921 aatactaagg ttaaaatgta gtaatacttt tggaacttgc aggtcagatt cttttatagg
10981 ggacacacta agggagcttg ggtgatagtt ggtaaaatgt gtttcaagtg atgaaaactt
11041 gaattattat caccgcaacc tactttttaa aaaaaaaagc caggcctgtt agagcatgct
11101 taagggatcc ctaggacttg ctgagcacac aagagtagtt acttggcagg ctcctggtga
11161 gagcatattt caaaaaacaa ggcagacaac caagaaacta cagttaaggt tacctgtctt
11221 taaaccatct gcatatacac agggatatta aaatattcca aataatattt cattcaagtt
11281 ttcccccatc aaattgggac atggatttct ccggtgaata ggcagagttg gaaactaaac
11341 aaatgttggt tttgtgattt gtgaaattgt tttcaagtga tagttaaagc ccatgagata
11401 cagaacaaag ctgctatttc gaggtctctt ggtttatact cagaagcact tctttgggtt
11461 tccctgcact atcctgatca tgtgctaggc ctaccttagg ctgattgttg ttcaaataaa
11521 cttaagtttc ctgtcaggtg atgtcatatg atttcatata tcaaggcaaa acatgttata
11581 tatgttaaac atttgtactt aatgtgaaag ttaggtcttt gtgggtttga tttttaattt
11641 tcaaaacctg agctaaataa gtcattttta catgtcttac atttggtgga attgtataat
11701 tgtggtttgc aggcaagact ctctgaccta gtaaccctac ctatagagca ctttgctggg
11761 tcacaagtct aggagtcaag catttcacct tgaagttgag acgttttgtt agtgtatact
11821 agtttatatg ttggaggaca tgtttatcca gaagatattc aggactattt ttgactgggc
11881 taaggaattg attctgatta gcactgttag tgagcattga gtggccttta ggcttgaatt
11941 ggagtcactt gtatatctca aataatgctg gcctttttta aaaagccctt gttctttatc
12001 accctgtttt ctacataatt tttgttcaaa gaaatacttg tttggatctc cttttgacaa
12061 caatagcatg ttttcaagcc atattttttt tccttttttt tttttttttt ggtttttcga
12121 gacagggttt ctctgtatag ccctggctgt cctggaactc actttgtaga ccaggctggc
12181 ctcgaactca gaaatccgcc tgcctctgcc tcctgagtgc cgggattaaa ggcgtgcacc
12241 accacgcctg gctaagttgg atattttgtt atataactat aaccaatact aactccactg
12301 ggtggatttt taattcagtc agtagtctta agtggtcttt attggccctt cattaaaatc
12361 tactgttcac tctaacagag gctgttggta ctagtggcac ttaagcaact tcctacggat
12421 atactagcag attaagggtc agggatagaa actagtctag cgttttgtat acctaccagc
12481 tttatactac cttgttctga tagaaatatt tcaggacatc tagcttatcg ataccgtcga
12541 cggtatcgat aagcttgata tcgaattcta ccgggtaggg gaggcgcttt tcccaaggca
12601 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
12661 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
12721 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
12781 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
12841 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
12901 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
12961 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
13021 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
13081 gtcctcgcca tggatcctga tgatgttgtt gattcttcta aatcttttgt gatggaaaac
13141 ttttcttcgt accacgggac taaacctggt tatgtagatt ccattcaaaa aggtatacaa
13201 aagccaaaat ctggtacaca aggaaattat gacgatgatt ggaaagggtt ttatagtacc
13261 gacaataaat acgacgctgc gggatactct gtagataatg aaaacccgct ctctggaaaa
13321 gctggaggcg tggtcaaagt gacgtatcca ggactgacga aggttctcgc actaaaagtg
13381 gataatgccg aaactattaa gaaagagtta ggtttaagtc tcactgaacc gttgatggag
13441 caagtcggaa cggaagagtt tatcaaaagg ttcggtgatg gtgcttcgcg tgtagtgctc
13501 agccttccct tcgctgaggg gagttctagc gttgaatata ttaataactg ggaacaggcg
13561 aaagcgttaa gcgtagaact tgagattaat tttgaaaccc gtggaaaacg tggccaagat
13621 gcgatgtatg agtatatggc tcaagcctgt gcaggaaatc gtgtcaggcg atctctttgt
13681 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag
13741 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt
13801 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcaga
13861 tcctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
13921 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
13981 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
14041 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
14101 gggctctatg gcttctgagg cggaaagaac cagctggggc tcgagggggg gcccggtacc
14161 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
14221 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
14281 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
14341 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
14401 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
14461 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
14521 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
14581 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
14641 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
14701 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
14761 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
14821 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
14881 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
14941 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
15001 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
15061 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
15121 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
15181 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
15241 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
15301 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
15361 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
15421 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
15481 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
15541 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
15601 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
15661 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
15721 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
15781 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
15841 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
15901 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
15961 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
16021 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
16081 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
16141 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
16201 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
16261 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
16321 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
16381 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
16441 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
16501 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
16561 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
16621 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
16681 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
16741 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
16801 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
16861 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
16921 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
16981 cgcgcgtaat acgactcact atagggcgaa ttggagctcc a
//
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Ai9质粒载体应用举例
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