pCMV-nCas9-P3-BFP

LOCUS       pCMV-nCas9-P3-BFP                8475 bp DNA     circular SYN 07-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 22, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        87..466
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     101..128
                     /label=OL1151
     primer_bind     437..466
                     /label=OL4541
     promoter        467..670
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     569..588
                     /label=OL790
     primer_bind     615..636
                     /label=OL096
     primer_bind     621..641
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(622..639)
                     /label=OL400
     primer_bind     622..639
                     /label=OL443
     primer_bind     640..663
                     /label=OL152
     primer_bind     640..663
                     /label=OL159
     primer_bind     649..671
                     /label=OL084
     primer_bind     712..731
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        712..730
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     712..730
                     /label=OL1124
     primer_bind     complement(727..746)
                     /label=OL1206
     primer_bind     complement(760..779)
                     /label=OL3635
     primer_bind     complement(774..800)
                     /label=OL1013
     primer_bind     complement(778..800)
                     /label=OL1036
     CDS             780..800
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     781..822
                     /label=OL1039
     primer_bind     781..820
                     /label=OL1296
     primer_bind     complement(781..800)
                     /label=OL1298
     CDS             801..4901
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=nCas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(805..824)
                     /label=OL1051
     primer_bind     complement(1442..1471)
                     /label=OL1321
     primer_bind     1447..1476
                     /label=OL1322
     primer_bind     1609..1623
                     /label=OL1270
     primer_bind     complement(1962..1996)
                     /label=OL1323
     primer_bind     1962..1996
                     /label=OL1324
     primer_bind     2521..2535
                     /label=OL1271
     primer_bind     3018..3057
                     /label=OL4211
     primer_bind     3267..3281
                     /label=OL1272
     primer_bind     complement(3300..3331)
                     /label=OL1316
     primer_bind     3300..3331
                     /label=OL1317
     primer_bind     4604..4623
                     /label=OL1328
     CDS             4902..4979
                     /label=SGGSx2-sv40NLS-SGGSx2
                     /note="/created_by=chenp"
     primer_bind     4902..4924
                     /label=OL1326
     primer_bind     4941..4957
                     /label=OL1153
     primer_bind     complement(4949..4965)
                     /label=OL1154
     primer_bind     complement(4954..4989)
                     /label=OL4542
     primer_bind     4958..4979
                     /label=OL1014
     primer_bind     complement(4959..4998)
                     /label=OL4212
     CDS             4959..4979
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     4971..5028
                     /label=OL4563
     primer_bind     4971..4990
                     /label=OL4565
     misc_feature    4980..5063
                     /label=P3
     primer_bind     4980..5063
                     /label=OL4213
     primer_bind     complement(4980..5063)
                     /label=OL4214
     primer_bind     complement(5014..5063)
                     /label=OL4564
     primer_bind     5045..5083
                     /label=OL4215
     primer_bind     complement(5045..5067)
                     /label=OL4279
     CDS             5064..5120
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     5064..5120
                     /label=OL866
     primer_bind     complement(5064..5120)
                     /label=OL867
     primer_bind     5064..5083
                     /label=OL3636
     primer_bind     5101..5146
                     /label=OL868
     CDS             5121..5816
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     primer_bind     complement(5795..5840)
                     /label=OL1314
     primer_bind     complement(5813..5837)
                     /label=OL4562
     primer_bind     5820..5849
                     /label=OL1014
     primer_bind     5820..5839
                     /label=OL1207
     primer_bind     5825..5848
                     /label=OL1035
     CDS             5825..5842
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     5825..5842
                     /label=OL1133
     primer_bind     complement(5825..5842)
                     /label=OL1152
     primer_bind     complement(5850..5869)
                     /label=OL4216
     primer_bind     complement(5865..5882)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5871..6095
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal
                     牛生长激素聚腺苷酸化信号
                     牛生长激素（BGH）多聚腺苷酸化信号能够保证高效的转录终止和mRNA的poly A结构"
     primer_bind     complement(5915..5932)
                     /label=OL698
     primer_bind     complement(6076..6095)
                     /label=OL1684
     primer_bind     complement(6166..6182)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6166..6182)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6179..6201)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6190..6206
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6214..6244)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6259..6280
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6397..6414)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6568..7156)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6648..6667)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(6750..6769)
                     /label=OL1041
     primer_bind     complement(6883..6902)
                     /label=OL631
     primer_bind     6939..6958
                     /label=OL798
     primer_bind     7200..7219
                     /label=OL659
     CDS             complement(7327..8187)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7950..7969
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     8103..8122
                     /label=OL1033
     primer_bind     complement(8103..8122)
                     /label=OL1034
     promoter        complement(8188..8292)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8371..8390)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
       61 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
      121 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      181 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      241 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      301 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      361 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      421 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      481 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      541 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      601 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      661 aagcagagct ggtttagtga accgtcagat ccgctagaga tccgcggccg ctaatacgac
      721 tcactatagg gagagccgcc accatgaaac ggacagccga cggaagcgag ttcgagtcac
      781 caaagaagaa gcggaaagtc gacaagaagt acagcatcgg cctggacatc ggcaccaact
      841 ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc
      901 tgggcaacac cgaccggcac agcatcaaga agaacctgat cggagccctg ctgttcgaca
      961 gcggcgaaac agccgaggcc acccggctga agagaaccgc cagaagaaga tacaccagac
     1021 ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg
     1081 acagcttctt ccacagactg gaagagtcct tcctggtgga agaggataag aagcacgagc
     1141 ggcaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca
     1201 tctaccacct gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg cggctgatct
     1261 atctggccct ggcccacatg atcaagttcc ggggccactt cctgatcgag ggcgacctga
     1321 accccgacaa cagcgacgtg gacaagctgt tcatccagct ggtgcagacc tacaaccagc
     1381 tgttcgagga aaaccccatc aacgccagcg gcgtggacgc caaggccatc ctgtctgcca
     1441 gactgagcaa gagcagaaag ctggaaaatc tgatcgccca gctgcccggc gagaagaaga
     1501 atggcctgtt cggaaacctg attgccctga gcctgggcct gacccccaac ttcaagagca
     1561 acttcgacct ggccgaggat gccaaactgc agctgagcaa ggacacctac gacgacgacc
     1621 tggacaacct gctggcccag atcggcgacc agtacgccga cctgtttctg gccgccaaga
     1681 acctgtccga cgccatcctg ctgagcgaca tcctgagagt gaacaccgag atcaccaagg
     1741 cccccctgag cgcctctatg atcaagagat acgacgagca ccaccaggac ctgaccctgc
     1801 tgaaagctct cgtgcggcag cagctgcctg agaagtacaa agagattttc ttcgaccaga
     1861 gcaagaacgg ctacgccggc tacattgacg gcggagccag ccaggaagag ttctacaagt
     1921 tcatcaagcc catcctggaa aagatggacg gcaccgagga actgctcgtg aagctgaaga
     1981 gagaggacct gctgcggaag cagcggacct tcgacaacgg cagcatcccc caccagatcc
     2041 acctgggaga gctgcacgcc attctgcggc ggcaggaaga tttttaccca ttcctgaagg
     2101 acaaccggga aaagatcgag aagatcctga ccttccgcat cccctactac gtgggccctc
     2161 tggccagggg aaacagcaga ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc
     2221 cctggaactt cgaggaagtg gtggacaagg gcgcttccgc ccagagcttc atcgagcgga
     2281 tgaccaactt cgataagaac ctgcccaacg agaaggtgct gcccaagcac agcctgctgt
     2341 acgagtactt caccgtgtat aacgagctga ccaaagtgaa atacgtgacc gagggaatga
     2401 gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga
     2461 ccaaccggaa agtgaccgtg aagcagctga aagaggacta cttcaagaaa atcgagtgct
     2521 tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa cgcctccctg ggcacatacc
     2581 acgatctgct gaaaattatc aaggacaagg acttcctgga caatgaggaa aacgaggaca
     2641 ttctggaaga tatcgtgctg accctgacac tgtttgagga cagagagatg atcgaggaac
     2701 ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat gaagcagctg aagcggcgga
     2761 gatacaccgg ctggggcagg ctgagccgga agctgatcaa cggcatccgg gacaagcagt
     2821 ccggcaagac aatcctggat ttcctgaagt ccgacggctt cgccaacaga aacttcatgc
     2881 agctgatcca cgacgacagc ctgaccttta aagaggacat ccagaaagcc caggtgtccg
     2941 gccagggcga tagcctgcac gagcacattg ccaatctggc cggcagcccc gccattaaga
     3001 agggcatcct gcagacagtg aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca
     3061 agcccgagaa catcgtgatc gaaatggcca gagagaacca gaccacccag aagggacaga
     3121 agaacagccg cgagagaatg aagcggatcg aagagggcat caaagagctg ggcagccaga
     3181 tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa cgagaagctg tacctgtact
     3241 acctgcagaa tgggcgggat atgtacgtgg accaggaact ggacatcaac cggctgtccg
     3301 actacgatgt ggacgctatc gtgcctcaga gctttctgaa ggacgactcc atcgacaaca
     3361 aggtgctgac cagaagcgac aagaaccggg gcaagagcga caacgtgccc tccgaagagg
     3421 tcgtgaagaa gatgaagaac tactggcggc agctgctgaa cgccaagctg attacccaga
     3481 gaaagttcga caatctgacc aaggccgaga gaggcggcct gagcgaactg gataaggccg
     3541 gcttcatcaa gagacagctg gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc
     3601 tggactcccg gatgaacact aagtacgacg agaatgacaa gctgatccgg gaagtgaaag
     3661 tgatcaccct gaagtccaag ctggtgtccg atttccggaa ggatttccag ttttacaaag
     3721 tgcgcgagat caacaactac caccacgccc acgacgccta cctgaacgcc gtcgtgggaa
     3781 ccgccctgat caaaaagtac cctaagctgg aaagcgagtt cgtgtacggc gactacaagg
     3841 tgtacgacgt gcggaagatg atcgccaaga gcgagcagga aatcggcaag gctaccgcca
     3901 agtacttctt ctacagcaac atcatgaact ttttcaagac cgagattacc ctggccaacg
     3961 gcgagatccg gaagcggcct ctgatcgaga caaacggcga aaccggggag atcgtgtggg
     4021 ataagggccg ggattttgcc accgtgcgga aagtgctgag catgccccaa gtgaatatcg
     4081 tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga gtctatcctg cccaagagga
     4141 acagcgataa gctgatcgcc agaaagaagg actgggaccc taagaagtac ggcggcttcg
     4201 acagccccac cgtggcctat tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca
     4261 agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat catggaaaga agcagcttcg
     4321 agaagaatcc catcgacttt ctggaagcca agggctacaa agaagtgaaa aaggacctga
     4381 tcatcaagct gcctaagtac tccctgttcg agctggaaaa cggccggaag agaatgctgg
     4441 cctctgccgg cgaactgcag aagggaaacg aactggccct gccctccaaa tatgtgaact
     4501 tcctgtacct ggccagccac tatgagaagc tgaagggctc ccccgaggat aatgagcaga
     4561 aacagctgtt tgtggaacag cacaagcact acctggacga gatcatcgag cagatcagcg
     4621 agttctccaa gagagtgatc ctggccgacg ctaatctgga caaagtgctg tccgcctaca
     4681 acaagcaccg ggataagccc atcagagagc aggccgagaa tatcatccac ctgtttaccc
     4741 tgaccaatct gggagcccct gccgccttca agtactttga caccaccatc gaccggaaga
     4801 ggtacaccag caccaaagag gtgctggacg ccaccctgat ccaccagagc atcaccggcc
     4861 tgtacgagac acggatcgac ctgtctcagc tgggaggtga ctccggcgga agctctggtg
     4921 gcagcaagcg gaccgccgac ggctctgaat tcgagagccc taagaagaaa agaaaggtgg
     4981 agatccagca gctggaggag gagatcgccc agctggagca gaagaacgcc gccctgaagg
     5041 agaagaacca ggccctgaag tacgctacta acttcagcct gctgaagcag gctggagacg
     5101 tggaggagaa ccctggacct agcgagctga ttaaggagaa catgcacatg aagctgtaca
     5161 tggagggcac cgtggacaac catcacttca agtgcacatc cgagggcgaa ggcaagccct
     5221 acgagggcac ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct
     5281 tcgacatcct ggctactagc ttcctctacg gcagcaagac cttcatcaac cacacccagg
     5341 gcatccccga cttcttcaag cagtccttcc ctgagggctt cacatgggag agagtcacca
     5401 catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc
     5461 tcatctacaa cgtcaagatc agaggggtga acttcacatc caacggccct gtgatgcaga
     5521 agaaaacact cggctgggag gccttcaccg agacgctgta ccccgctgac ggcggcctgg
     5581 aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag ccatctgatc gcaaacatca
     5641 agaccacata tagatccaag aaacccgcta agaacctcaa gatgcctggc gtctactatg
     5701 tggactacag actggaaaga atcaaggagg ccaacaacga gacctacgtc gagcagcacg
     5761 aggtggcagt ggccagatac tgcgacctcc ctagcaaact ggggcacaag cttaattagc
     5821 cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga ctgtgccttc
     5881 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     5941 cactcccact gtcctttcct aataaaatga gaaaattgca tcgcattgtc tgagtaggtg
     6001 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     6061 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     6121 gggctcgata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     6181 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6241 taaagcctag ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6301 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6361 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6421 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6481 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6541 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6601 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6661 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6721 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6781 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6841 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6901 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6961 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7021 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7081 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7141 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgaca ctcagtggaa
     7201 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7261 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7321 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7381 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7441 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7501 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7561 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7621 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7681 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7741 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7801 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7861 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7921 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     7981 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8041 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8101 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8161 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8221 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8281 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatcg
     8341 atctcccgat cccctagggt ctactctcag tacaatctgc tctgatgccg catagttaag
     8401 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
     8461 agctacaaca aggca
//