AatII-pRPS5A-PTP-3Xflag-rbc L 294 Right_G1397 C-ABE

LOCUS      AatII-pRPS5A-PTP-3Xflag-rbc L 294 Right_G1397 C-ABE              13148 bp ds-DNA     circular SYN 01-SEP-2022
DEFINITION  rbc L 294 Right_G1397 C-ABE.
ACCESSION   .
VERSION     .
KEYWORDS    AatII-pRPS5A-PTP-3Xflag-rbc L 294 Right_G1397 C-ABE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13148)
  TITLE     sTALED for plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 1, 2022 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..13148
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(5..25)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             1857..1922
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5335..5359
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6690..7319
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7748..8821
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8887..9081
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9317..9339)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9425..9565
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9480..9499
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9580..9597)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9751..10339)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9831..9850)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10585..11376)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    11897..11921
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      12014..12266
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(12286..12837)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(12879..13058)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 cgttcaactt ttctatacaa agttgagacg tcgactcaac ttttgattcg ctatttgcag
       61 tgcacctgtg gcgttcatca catcttttgt gacactgttt gcactggtca ttgctattac
      121 aaaggacctt cctgatgttg aaggagatcg aaagtaagta actgcacgca taaccatttt
      181 ctttccgctc tttggctcaa tccatttgac agtcaaagac aatgtttaac cagctccgtt
      241 tgatatattg tctttatgtg tttgttcaag catgtttagt taatcatgcc tttgattgat
      301 cttgaatagg ttccaaatat caaccctggc aacaaaactt ggagtgagaa acattgcatt
      361 cctcggttct ggacttctgc tagtaaatta tgtttcagcc atatcactag ctttctacat
      421 gcctcaggtg aattcatcta tttccgtctt aactatttcg gttaattaaa gcacgaacac
      481 cattactgca tgtagaagct tgataaacta tcgccaccaa tttatttttg ttgcgatatt
      541 gttactttcc tcagtatgca gctttgaaaa gaccaaccct cttatccttt aacaatgaac
      601 aggtttttag aggtagcttg atgattcctg cacatgtgat cttggcttca ggcttaattt
      661 tccaggtaaa gcattatgag atactcttat atctcttaca tacttttgag ataatgcaca
      721 agaacttcat aactatatgc tttagtttct gcatttgaca ctgccaaatt cattaatctc
      781 taatatcttt gttgttgatc tttggtagac atgggtacta gaaaaagcaa actacaccaa
      841 ggtaaaatac ttttgtacaa acataaactc gttatcacgg aacatcaatg gagtgtatat
      901 ctaacggagt gtagaaacat ttgattattg caggaagcta tctcaggata ttatcggttt
      961 atatggaatc tcttctacgc agagtatctg ttattcccct tcctctagct ttcaatttca
     1021 tggtgaggat atgcagtttt ctttgtatat cattcttctt cttctttgta gcttggagtc
     1081 aaaatcggtt ccttcatgta catacatcaa ggatatgtcc ttctgaattt ttatatcttg
     1141 caataaaaat gcttgtacca attgaaacac cagctttttg agttctatga tcactgactt
     1201 ggttctaacc aaaaaaaaaa aaatgtttaa tttacatatc taaaagtagg tttagggaaa
     1261 cctaaacagt aaaatatttg tatattattc gaatttcact catcataaaa acttaaattg
     1321 caccataaaa ttttgtttta ctattaatga tgtaatttgt gtaacttaag ataaaaataa
     1381 tattccgtaa gttaaccggc taaaaccacg tataaaccag ggaacctgtt aaaccggttc
     1441 tttactggat aaagaaatga aagcccatgt agacagctcc attagagccc aaaccctaaa
     1501 tttctcatct atataaaagg agtgacatta gggtttttgt tcgtcctctt aaagcttctc
     1561 gttttctctg ccgtctctct cattcgcgcg acgcaaacga tcttcaggtg atcttctttc
     1621 tccaaatcct ctctcataac tctgatttcg tacttgtgta tttgagctca cgctctgttt
     1681 ctctcaccac agccatggat tcacagctag tcttgtctct gaagctgaat ccaagcttca
     1741 ctcctctttc tcctctcttc cctttcactc catgttcttc tttttcgccg tcgctccggt
     1801 tttcttcttg ctactcccgc cgcctctatt ctccggttac cgtctacgcc gcgaaggact
     1861 acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat gacgatgaca
     1921 agcccgggga tctacgcacg ctcggctaca gccagcagca acaggagaag atcaaaccga
     1981 aggttcgttc gacagtggcg cagcaccacg aggcactggt cggccatggg tttacacacg
     2041 cgcacatcgt tgcgctcagc caacacccgg cagcgttagg gaccgtcgct gtcaagtatc
     2101 aggacatgat cgcagcgttg ccagaggcga cacacgaagc gatcgttggc gtcggcaaac
     2161 agtggtccgg cgcacgcgct ctggaggcct tgctcacggt ggcgggagag ttgagaggtc
     2221 caccgttaca gttggacaca ggccaacttc tcaagattgc aaaacgtggc ggcgtgaccg
     2281 cagtggaggc agtgcatgca tggcgcaatg cactgacggg tgcccccctg aaccttaccc
     2341 ctgatcaagt tgtcgctatt gctagtaata ttggaggaaa gcaggcactt gaaactgtcc
     2401 aacggcttct ccctgtgctc tgtcaagacc acggtctgac tccagcacaa gtcgtggcaa
     2461 tcgcctcaaa tggcggaggt aaacaggcac ttgaaactgt gcagcggctg ttgccagtgt
     2521 tgtgtcaagc tcatggcctc actcctgccc aggtggttgc catcgcatcc catgatggtg
     2581 ggaagcaagc tctcgagaca gtgcaacgcc tgcttcccgt cttgtgtcag gaccatgggt
     2641 tgacccccga tcaggtggtt gccatcgcat caaatatcgg tgggaaacaa gcacttgaga
     2701 ctgtccaacg gttgttgcct gtcctctgcc aggctcatgg attgactccc gaccaggttg
     2761 tcgctattgc ttcaaacatt ggaggaaagc aggccttgga gactgtgcag cggcttcttc
     2821 ctgtcctttg tcaagaccat ggcctgaccc ccgaccaggt ggtggccatc gccagcaacg
     2881 gaggcggcaa gcaggccctg gaaaccgtgc agagactgct gcccgtgctg tgccaggccc
     2941 acggtcttac accagatcag gtggtggcta tcgctagtaa tattggagga aagcaagccc
     3001 tcgaaaccgt tcaacggctg ttgccagtgc tctgtcaggc ccatggtctt acccccgacc
     3061 aggttgtggc aattgcttcc aacatcggcg ggaagcaagc actcgagaca gtgcaaagac
     3121 ttcttcccgt tctctgtcaa gctcacggat tgacccctgc acaagtcgtc gccattgcca
     3181 gccatgacgg gggtaaacaa gccctcgaga ctgtccaacg cttgctccct gtgttgtgcc
     3241 aagcccacgg cctcacacca gctcaggttg ttgccatcgc tagcaatatt ggtgggaaac
     3301 aggctcttga aaccgttcaa cgcctccttc ctgtgctctg tcaagaccat ggtcttacac
     3361 ccgatcaagt cgtggctatt gcttcaaaca acggaggaaa gcaagcactt gagactgtcc
     3421 aacgccttct gccagtcctc tgccaggatc atggcctgac tccagaacaa gttgtggcaa
     3481 tcgcatctca cgatggcggg aaacaggcat tggaaaccgt tcaaaggttg ctgccagtcc
     3541 tttgtcaggc ccacggcctg actcccgaac aagtcgtcgc tattgcaagc aacaacgggg
     3601 ggaaacaggc cctggagact gtccagcggc tgttgcctgt tctctgtcag gcacatggcc
     3661 tcacccctga ccaagtggtg gctatcgcat ctaacggcgg tggcaagcag gccttggaaa
     3721 cagtgcaaag gcttctgcca gtcttgtgcc aggatcatgg gctcacacca gctcaggttg
     3781 ttgcaatcgc cagtaataac ggaggtaagc aagctctgga aaccgtgcag aggttgctgc
     3841 ctgttctgtg tcaagctcat ggcctcactc ctgcccaggt cgttgccatt gcaagtcatg
     3901 acggcggcaa gcaagcattg gagacagtgc agagactctt gcccgtcctg tgtcaagacc
     3961 atgggttgac tcctgatcag gtcgtcgcaa ttgcctccaa tatcgggggc aaacaggcct
     4021 tggagacagt ccaacgcttg ctcccagttc tttgccaaga tcacggcctt acacccgcac
     4081 aggtggtcgc catcgctagt aatggtgggg gcaaacaggc tctcgagact gttcaacggc
     4141 ttctcccagt tttgtgtcaa gcccacggtc tgactccgga acaggtggtg gcgattgcaa
     4201 gcaacggcgg cggcaaacag gctctagaga gcattgttgc ccagctctcc agacctgatc
     4261 cggcgctagc cgcgttgaca aatgatcatc ttgttgctct tgcttgtctt ggcggacgac
     4321 ctgccctgga tgctgtgaaa aaggggttgg ggggatccgg cagtgccata cctgtgaagc
     4381 ggggagcaac aggggagaca aaggtgttca caggcaactc taacagtcca aagagcccca
     4441 ccaaaggcgg gtgttcagga agcgaaactc ctggtacctc agagtccgct actcccgaat
     4501 cctctgaggt ggagttttcc cacgagtact ggatgagaca tgccctgacc ctggccaaga
     4561 gggcacggga tgagagggag gtgcctgtgg gagccgtgct ggtgctgaac aatagagtga
     4621 tcggcgaggg ctggaacaga gccatcggcc tgcacgaccc aacagcccat gccgaaatta
     4681 tggccctgag acagggcggc ctggtcatgc agaactacag actgattgac gccaccctgt
     4741 acgtgacatt cgagccttgc gtgatgtgcg ccggcgccat gatccactct aggatcggcc
     4801 gcgtggtgtt tggcgtgagg aactcaaaaa gaggcgccgc aggctccctg atgaacgtgc
     4861 tgaactaccc cggcatgaat caccgcgtcg aaattaccga gggaatcctg gcagatgaat
     4921 gtgccgccct gctgtgcgat ttctatcgga tgcctagaca ggtgttcaat gctcagaaga
     4981 aggcccagag ctccatcaac tagggtacct gataatcgat atcccgcggc catgctagag
     5041 tccgcaaaaa tcaccagtct ctctctacaa atctatctct ctctattttt ctccagaata
     5101 atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg ctcatgtgtt
     5161 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat
     5221 ttctaattcc taaaaccaaa atccagtgac ctgcaggcat gccgtcgggc ccaagcttag
     5281 cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag
     5341 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
     5401 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
     5461 ccctcgggat caaagtactt taaagtactt taaagtactt taaagtactt tgatccaacc
     5521 cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga
     5581 catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt
     5641 tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta
     5701 cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga
     5761 ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc
     5821 cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct
     5881 acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga
     5941 cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga
     6001 gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat
     6061 tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa
     6121 ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc
     6181 ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt
     6241 gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc
     6301 caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc
     6361 cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg
     6421 tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg
     6481 cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag
     6541 ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg
     6601 tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga
     6661 gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag
     6721 gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg
     6781 ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc
     6841 gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga
     6901 aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg
     6961 ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg
     7021 acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg
     7081 gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg
     7141 aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc
     7201 gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg
     7261 gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag
     7321 ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag
     7381 cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg
     7441 ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca
     7501 aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag
     7561 caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag
     7621 aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag
     7681 gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc aagcccgagg
     7741 aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga
     7801 tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga
     7861 agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca
     7921 accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga
     7981 ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt
     8041 ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct
     8101 tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta
     8161 cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg
     8221 gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg
     8281 ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa
     8341 caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt
     8401 atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc
     8461 ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa
     8521 cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt
     8581 tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac
     8641 gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa
     8701 gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg
     8761 cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta
     8821 atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct
     8881 ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc
     8941 gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat
     9001 aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa
     9061 aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc
     9121 gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc
     9181 cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc
     9241 cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg
     9301 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     9361 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     9421 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
     9481 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     9541 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
     9601 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     9661 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     9721 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     9781 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     9841 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     9901 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     9961 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    10021 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    10081 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    10141 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
    10201 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    10261 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    10321 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    10381 gaacgaaaac tcacgttaag ggattttggt catgcatgat atatctccca atttgtgtag
    10441 ggcttattat gcacgcttaa aaataataaa agcagacttg acctgatagt ttggctgtga
    10501 gcaattatgt gcttagtgca tctaacgctt gagttaagcc gcgccgcgaa gcggcgtcgg
    10561 cttgaacgaa tttctagcta gacattattt gccgactacc ttggtgatct cgcctttcac
    10621 gtagtggaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc
    10681 aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca ggcgctccat
    10741 tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc tgtaccaaat
    10801 gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca
    10861 tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag
    10921 ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat
    10981 agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt cattgcgctg
    11041 ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg
    11101 cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg aagccgaagt
    11161 ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt
    11221 aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg agccgtacaa
    11281 atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag
    11341 ttgagtcgat acttcggcga tcaccgcttc ccccatgatg tttaactttg ttttagggcg
    11401 actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta
    11461 acgcgcttgc tgcttggatg cccgaggcat agactgtacc ccaaaaaaac agtcataaca
    11521 agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca
    11581 gttgcgtgag cgcatacgct acttgcatta cagcttacga accgaacagg cttatgtcca
    11641 ctgggttcgt gcccgaattg atcacaggca gcaacgctct gtcatcgtta caatcaacat
    11701 gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga
    11761 atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt
    11821 cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga
    11881 gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac
    11941 ttaataacac attgcggacg tttttaatgt actgaattaa cgccgaattg aattatcagc
    12001 ttgcatgccg gtcgatctag taacatagat gacaccgcgc gcgataattt atcctagttt
    12061 gcgcgctata ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata
    12121 aaaacccatc tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa
    12181 ttcaacagaa attatatgat aatcatcgca agaccggcaa caggattcaa tcttaagaaa
    12241 ctttattgcc aaatgtttga acgatctgct tgactctagg ggtcatcaga tttcggtgac
    12301 gggcaggacc ggacggggcg gcaccggcag gctgaagtcc agctgccaga aacccacgtc
    12361 atgccagttc ccgtgcttga agccggccgc ccgcagcatg ccgcgggggg catatccgag
    12421 cgcctcgtgc atgcgcacgc tcgggtcgtt gggcagcccg atgacagcga ccacgctctt
    12481 gaagccctgt gcctccaggg acttcagaag gtgggtgtag agcgtggagc ccagtcccgt
    12541 ccgctggtgg cggggggaga cgtacacggt cgactcggcc gtccagtcgt aggcgttgcg
    12601 tgccttccag ggacccgcgt aggcgatgcc ggcgacctcg ccgtccacct cggcgacgag
    12661 ccagggatag cgctcccgca gacggacgag gtcgtccgtc cactcctgcg gttcctgcgg
    12721 ctcggtacgg aagttgaccg tgcttgtctc gatgtagtgg ttgacgatgg tgcagaccgc
    12781 cggcatgtcc gcctcggtgg cacggcggat gtcggccggg cgtcgttctg ggctcatggt
    12841 agatcccctc gatcgagttg agagtgaata tgagactcta attggatacc gaggggaatt
    12901 tatggaacgt cagtggagca tttttgacaa gaaatatttg ctagctgata gtgaccttag
    12961 gcgacttttg aacgcgcaat aatggtttct gacgtatgtg cttagctcat taaactccag
    13021 aaacccgcgg ctcagtggct ccttcaacgt tgcggttctg tcagttccaa acgtaaaacg
    13081 gcttgtcccg cgtcatcggc gggggtcata acgtgactcc cttaattctc cgctcatgat
    13141 cgataatt
//