| 质粒名称: | pAAV-CAG-mRubyFT-P2A-EGFP |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000187 |
| 产品用途: | Expresses the mRubyFT blue-to-red fluorescent timer in mammalian cells |
| 存储实验室: | Laboratory of Molecular Design |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Mach1 |
|
插入基因信息 |
|
| 插入基因名称: | mRubyFT |
| 物种来源: | Synthetic |
| 密码子优化: | 细菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
The mRubyFT Protein, Genetically Encoded Blue-to-Red Fluorescent Timer
Subach O.M., Tashkeev A., Vlaskina A.V., Petrenko D.E., Gaivoronskii F.A., Nikolaeva A.Y., Ivashkina O.I., Anokhin K.V., Popov V.O., Boyko K.M., Subach F.V.
Int. J. Mol. Sci. 23, 3208 (2022)
|
买家导航
pAAV-CAG-mRubyFT-P2A-EGFP载体质粒基本信息
pAAV-CAG-mRubyFT-P2A-EGFP质粒图谱载体图谱和pAAV-CAG-mRubyFT-P2A-EGFP载体序列质粒序列多克隆位点信息
pAAV-CAG-mRubyFT-P2A-EGFP质粒载体简介
pAAV-CAG-mRubyFT-P2A-EGFP质粒序列载体序列
LOCUS pAAV-CAG-mRubyFT-P2A-EGFP 6262 bp DNA circular SYN 22-APR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6262)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 29, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6262
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(182..770)
/direction=LEFT
/label=ori
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(941..1801)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(1802..1906)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1932..2387)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2911..3188
/label=chicken beta-actin promoter
regulatory 3616..3625
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 3622..4329
/codon_start=1
/label=mRubyFT
/translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
RIKVIEGGPLPFAFDILATSFLYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
VVTVMQDTSLEDGCLVYHVQVRGVDFPSNGPVMQKKTKGWEPSTEMMYPADGGLRGYTH
MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVLREHSV
AKFAGRGGMDELYK"
primer_bind 3862..3882
/label=mRubyFT-F
CDS 4354..4410
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 4423..5136
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
misc_feature 5164..5752
/label=WPRE
/note="woodchuck posttranscriptional regulatory element"
CDS complement(5635..5646)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 5795..5916
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
ORIGIN
1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
2641 gaagatcgta ggttaattaa ccattgacgt caataatgac gtatgttccc atagtaacgc
2701 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
2761 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
2821 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
2881 tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc
2941 tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt
3001 gtgcagcgat gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg
3061 aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc
3121 gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc
3181 ggcgggcggg agtcgctgcg acgctgcctt cgccccgtgc cccgctccgc cgccgcctcg
3241 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
3301 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
3361 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
3421 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg
3481 gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct
3541 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattggg
3601 gatccggtac cggtcgccac catggtgtct aagggcgaag agctgatcaa ggaaaatatg
3661 cgtatgaagg tggtcatgga aggttcggtc aacggccacc aattcaaatg cacaggtgaa
3721 ggagaaggca atccgtacat gggaactcaa accatgagga tcaaagtcat cgagggagga
3781 cccctgccat ttgcctttga cattcttgcc acgtcgttcc tgtatggcag ccgtactttt
3841 atcaagtacc cgaaaggcat tcctgatttc tttaaacagt cctttcctga gggttttact
3901 tgggaaagag ttacgagata cgaagatggt ggagtcgtca ccgtcatgca ggacaccagc
3961 cttgaggatg gctgtctcgt ttaccacgtc caagtaagag gggtagactt tccctccaat
4021 ggtcccgtga tgcagaagaa gaccaagggt tgggagcctt ctacagagat gatgtatcca
4081 gcagatggtg gtctgagggg atacactcat atggcactga aagttgatgg tggtggccat
4141 ctgtcttgct ctttcgtaac aacttacagg tcaaaaaaga ccgtcgggaa catcaagatg
4201 cccggtatcc atgccgttga tcaccgcctg gaaaggttag aggaaagtga caatgaaatg
4261 ttcgtagtac tacgcgaaca ctctgttgcc aagttcgccg ggcgtggtgg catggacgag
4321 ctgtacaagc gaccggttca cgtgggaagt ggggctacaa acttctcact gctgaaacag
4381 gccggggatg tggaggaaaa cccaggacca cacgtgacta gtgtgagcaa gggcgaggag
4441 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
4501 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
4561 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
4621 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
4681 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
4741 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
4801 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
4861 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
4921 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
4981 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
5041 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
5101 gccgggatca ctctcggcat ggacgagctg tacaagtaag aattcgatat caagcttatc
5161 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
5221 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
5281 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
5341 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
5401 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
5461 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
5521 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
5581 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
5641 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
5701 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
5761 cgtcgacccg ggcggccgct tcgagcagac atgataagat acattgatga gtttggacaa
5821 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
5881 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
5941 atgtttcagg ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa
6001 tgtggtaaaa tcgataagga tcttcctaga gcatggctac gtagataagt agcatggcgg
6061 gttaatcatt aactacaagg aacccctagt gatggagttg gccactccct ctctgcgcgc
6121 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
6181 ggcctcagtg agcgagcgag cgcgcagctg cattaatgaa tcggccaacg cgcggggaga
6241 ggcggtttgc gtattgggcg ct
//
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