| 质粒名称: | pTaU6-esgRNA |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 115630 |
| 存储实验室: | Caixia Gao |
|
载体骨架基本信息 |
|
| 载体名称: | pUC58 |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 3128bp |
| 载体类型: | CRISPR |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: |
TaU6p-esgRNA
gRNA/shRNA
|
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | Unknown (unknown if destroyed) |
| 3’克隆位点: | Unknown (unknown if destroyed) |
|
文献引用方法 |
|
| 材料和方法部分: | pTaU6-esgRNA was a gift from Caixia Gao (Addgene plasmid # 115630 ; http://n2t.net/addgene:115630 ; RRID:Addgene_115630) |
| 参考文献部分: | Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Li C, Zong Y, Wang Y, Jin S, Zhang D, Song Q, Zhang R, Gao C. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. 10.1186/s13059-018-1443-z PubMed 29807545 |
买家导航
pTaU6-esgRNA载体质粒基本信息
pTaU6-esgRNA质粒图谱载体图谱和pTaU6-esgRNA载体序列质粒序列多克隆位点信息
pTaU6-esgRNA质粒载体简介
pTaU6-esgRNA质粒序列载体序列
LOCUS pTaU6-esgRNA 3128 bp DNA circular SYN 14-APR-2025
DEFINITION expression esgRNA in wheat protoplasts.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3128)
AUTHORS Li C, Zong Y, Wang Y, Jin S, Zhang D, Song Q, Zhang R, Gao C
TITLE Expanded base editing in rice and wheat using a Cas9-adenosine
deaminase fusion.
JOURNAL Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.
PUBMED 29807545
REFERENCE 2 (bases 1 to 3128)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Genome
Biol."; date: "2018-05-29"; volume: "19(1):59. doi"; pages: "
10.1186/s13059-018-1443-z"
COMMENT Sequence Label: pTaU6-esgRNA
FEATURES Location/Qualifiers
source 1..3128
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 127..149
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 141..158
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 142..158
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
terminator 632..651
/label=SUP4 terminator
/note="transcription terminator for the S. cerevisiae SUP4
tRNA gene"
primer_bind complement(670..686)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(670..686)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(683..705)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 694..710
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(718..724,725..742,743..748))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 763..784
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(901..918)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1072..1660)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1152..1171)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(1831..2622,2623..2691))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 2454..2473
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(2692..2796)
/gene="bla"
/label=AmpR promoter
primer_bind 2864..2882
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(2920..2942)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 3042..3061
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
121 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attcgaccaa gcccgttatt
181 ctgacagttc tggtgctcaa cacatttata tttatcaagg agcacattgt tactcactgc
241 taggagggaa tcgaactagg aatattgatc agaggaacta cgagagagct gaagataact
301 gccctctagc tctcactgat ctgggcgcat agtgagatgc agcccacgtg agttcagcaa
361 cggtctagcg ctgggctttt aggcccgcat gatcgggctt tgtcgggtgg tcgacgtgtt
421 cacgattggg gagagcaacg cagcagttcc tcttagttta gtcccacctc gcctgtccag
481 cagagttctg accggtttat aaactcgctt gctgcatcag acttgtgaga ccctgcaggg
541 tctctgttta agagctatgc tggaaacagc atagcaagtt taaataaggc tagtccgtta
601 tcaacttgaa aaagtggcac cgagtcggtg ctttttttgt tttttatgtc taagcttggc
661 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
721 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
781 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
841 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
901 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
961 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
1021 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
1081 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
1141 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
1201 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
1261 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
1321 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
1381 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
1441 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
1501 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
1561 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
1621 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
1681 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
1741 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
1801 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
1861 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
1921 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagaaccacg
1981 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
2041 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
2101 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
2161 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
2221 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
2281 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
2341 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
2401 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
2461 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
2521 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
2581 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
2641 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
2701 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
2761 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
2821 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
2881 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
2941 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
3001 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
3061 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
3121 catcaggc
//
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