| 质粒名称: | pAAV-CAG-iRFP670 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000052 |
| 产品用途: | AAV, Expresses iRFP670 in cytoplasm of mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a, TOP10 |
|
插入基因信息 |
|
| 插入基因名称: |
iRFP670
|
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Quantitative assessment of near-infrared fluorescent proteins
Zhang H., Papadaki S., Sun X., Wang X., Drobizhev M., Yao L., Rehbock M., Köster R.W., Wu L., Namikawa K., Piatkevich K.D.
Nat. Methods 2023 2010 20, 1605–1616 (2023)
|
pAAV-CAG-iRFP670
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-CAG-iRFP670载体质粒基本信息
pAAV-CAG-iRFP670质粒图谱载体图谱和pAAV-CAG-iRFP670载体序列质粒序列多克隆位点信息
pAAV-CAG-iRFP670质粒载体简介
pAAV-CAG-iRFP670质粒序列载体序列
LOCUS pAAV-CAG-iRFP670 5659 bp DNA circular SYN 21-APR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5659)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Jun 25, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5659
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 279..556
/label=chicken beta-actin promoter
regulatory 977..982
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 983..1915
/codon_start=1
/product="near-infrared fluorescent protein with an
emission peak at 670 nm, engineered from a bacterial
phytochrome (Shcherbakova and Verkhusha, 2013)"
/label=iRFP670
/note="derived from Rhodopseudomonas palustris RpBphP6"
/translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
primer_bind complement(1896..1944)
/label=0503-iRFP670-R
misc_feature 1943..2531
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(2414..2425)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 2574..2695
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3223..3811)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3982..4842)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4843..4947)
/gene="bla"
/label=AmpR promoter
rep_origin complement(4973..5428)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
61 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
241 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
301 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
361 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
421 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
481 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
541 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
601 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
661 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
721 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
781 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
841 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
901 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
961 aattggatcc ggtaccgcca ccatggcgcg taaggtcgat ctcacctcct gcgatcgcga
1021 gccgatccac atccccggca gcattcagcc gtgcggctgc ctgctagcct gcgacgcgca
1081 ggcggtgcgg atcacgcgca ttacggaaaa tgccggcgcg ttctttggac gcgaaactcc
1141 gcgggtcggt gagctactcg ccgattactt cggcgagacc gaagcccatg cgctgcgcaa
1201 cgcactggcg cagtcctccg atccaaagcg accggcgctg atcttcggtt ggcgcgacgg
1261 cctgaccggc cgcaccttcg acatctcact gcatcgccat gacggtacat cgatcatcga
1321 gttcgagcct gcggcggccg aacaggccga caatccgctg cggctgacgc ggcagatcat
1381 cgcgcgcacc aaagaactga agtcgctcga agagatggcc gcacgggtgc cgcgctatct
1441 gcaggcgatg ctcggctatc accgcgtgat gttgtaccgc ttcgcggacg acggctccgg
1501 gatggtgatc ggcgaggcga agcgcagcga cctcgagagc tttctcggtc agcactttcc
1561 ggcgtcgctg gtcccgcagc aggcgcggct actgtacttg aagaacgcga tccgcgtggt
1621 ctcggattcg cgcggcatca gcagccggat cgtgcccgag cacgacgcct ccggcgccgc
1681 gctcgatctg tcgttcgcgc acctgcgcag catctcgccc tgccatctcg aatttctgcg
1741 gaacatgggc gtcagcgcct cgatgtcgct gtcgatcatc attgacggca cgctatgggg
1801 attgatcatc tgtcatcatt acgagccgcg tgccgtgccg atggcgcagc gcgtcgcggc
1861 cgaaatgttc gccgacttct tatcgctgca cttcaccgcc gcccaccacc aacgctaaga
1921 attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
1981 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
2041 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
2101 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
2161 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
2221 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
2281 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
2341 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
2401 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
2461 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
2521 cgcctccccg catcgatacc gtcgacccgg gcggccgctt cgagcagaca tgataagata
2581 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
2641 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
2701 caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag
2761 caagtaaaac ctctacaaat gtggtaaaat cgataaggat cttcctagag catggctacg
2821 tagataagta gcatggcggg ttaatcatta actacaagga acccctagtg atggagttgg
2881 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
2941 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc attaatgaat
3001 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
3061 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
3121 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
3181 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
3241 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
3301 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
3361 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
3421 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
3481 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
3541 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
3601 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
3661 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
3721 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
3781 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
3841 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
3901 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
3961 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
4021 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
4081 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
4141 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
4201 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
4261 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
4321 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
4381 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
4441 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
4501 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
4561 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
4621 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
4681 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
4741 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
4801 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
4861 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
4921 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
4981 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
5041 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
5101 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
5161 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
5221 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
5281 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
5341 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
5401 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
5461 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tgcgcgctcg ctcgctcact
5521 gaggccgccc gggcaaagcc cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc
5581 gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttcctt gtagttaatg
5641 attaacccgc catgctact
//
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pAAV-CAG-iRFP670质粒载体应用举例
pAAV-CAG-iRFP670-P2A-GFP
2026-06-22
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