pAAV-Syn-pHluorin-SYT1_I368T

LOCUS       pAAV-Syn-pHluorin-SYT1_I368T               6574 bp DNA     circular SYN 03-MAR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6574)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Aug 27, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    311..800
                     /label=Syn
     promoter        349..796
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     primer_bind     734..755
                     /label=Syn-F
     misc_feature    812..817
                     /label=Kozak sequence
     CDS             818..907
                     /codon_start=1
                     /product="signal sequence from bovine prolactin"
                     /label=prolactin signal sequence
                     /translation="MDSKGSSQKGSRLLLLLVVSNLLLCQGVVS"
     CDS             926..1636
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(953..981)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1576..1596
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             1679..2947
                     /codon_start=1
                     /label=SYT1/I368T
                     /translation="MVSESHHEALAAPPVTTVATVLPSNATEPASPGEGKEDAFSKLKE
                     KFMNELHKIPLPPWALIAIAIVAVLLVLTCCFCICKKCLFKKKNKKKGKEKGGKNAINM
                     KDVKDLGKTMKDQALKDDDAETGLTDGEEKEEPKEEEKLGKLQYSLDYDFQNNQLLVGI
                     IQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQFTFKVPYSELGG
                     KTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDICFSL
                     RYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYY
                     NESFSFEVPFEQIQKVQVVVTVLDYDKTGKNDAIGKVFVGYNSTGAELRHWSDMLANPR
                     RPIAQWHTLQVEEEVDAMLAVKK"
     misc_feature    1694..2017
                     /label=N-terminal domain
     misc_feature    2105..2473
                     /label=C2A domain
     misc_feature    2498..2905
                     /label=C2B domain
     misc_feature    2780..2782
                     /label=Ile368Thr
     misc_feature    2972..3560
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(3443..3454)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3578..3785
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3837..3977
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4052..4507
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4789..4893
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4894..5754
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5925..6513
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgcaaa gatggataaa gttttaaaca gagaggaatc
      181 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
      241 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcagca
      301 aattaattaa tggttaatta atctagactg cagagggccc tgcgtatgag tgcaagtggg
      361 ttttaggacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc ccgacccact
      421 ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga gggggagggg
      481 aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga cagtgccttc
      541 gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc tgacgtcact
      601 cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg ccgccgccgg
      661 cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg accatctgcg
      721 ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag gagtcgtgtc
      781 gtgcctgaga gcgcagtcga gaggatccta agccaccatg gacagcaaag gttcgtcgca
      841 gaaagggtcc cgcctgctcc tgctgctggt ggtgtcaaat ctactcttgt gccagggtgt
      901 ggtctccgat atcggtacca cgcgtagtaa aggcgaagaa cttttcactg gagttgtccc
      961 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
     1021 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
     1081 acctgttcct tggccaacac ttgtcactac tttaacttat ggtgttcaat gcttttcaag
     1141 atacccagat catatgaaac ggcatgactt tttcaagagt gccatgcccg aaggttatgt
     1201 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa
     1261 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
     1321 tggaaacatt cttggacaca aattggaata caactataac gatcaccagg tgtacatcat
     1381 ggcagacaaa caaaagaatg gaatcaaagc taacttcaaa attagacaca acattgaaga
     1441 tggaggcgtt caactagcag accattatca acaaaatact ccaattggcg atgggcccgt
     1501 ccttttacca gacaaccatt acctgtttac aacttctact ctttcgaaag atcccaacga
     1561 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
     1621 ggatgaacta tacaaagaat tctcaggcgg aagcggaggc tccggcggga gcggcggaat
     1681 ggtgagcgag agtcaccatg aggccctggc agccccgcct gtcaccactg tcgcgactgt
     1741 tctgccaagc aatgccacag agccagccag tcctggagaa ggaaaggaag atgcattttc
     1801 taagctgaag gagaagttta tgaatgagtt gcataaaatt ccattgccac cgtgggcctt
     1861 aattgcaata gccatagtcg cagtcctttt agtcctgacc tgctgctttt gtatctgtaa
     1921 gaaatgtttg ttcaaaaaga aaaacaagaa gaagggaaag gaaaaaggag ggaagaatgc
     1981 cattaacatg aaagatgtaa aagacttagg gaagacgatg aaagatcagg ccctcaagga
     2041 tgatgatgct gaaactggat tgacagatgg agaagaaaaa gaagaaccca aagaagagga
     2101 gaaactggga aaacttcagt attcactgga ttatgatttc caaaataacc agctgctggt
     2161 agggatcatt caggctgccg aactgcccgc cttggacatg gggggcacat ctgatcctta
     2221 cgtgaaagtg tttctgctac ctgataagaa gaagaaattt gagacaaaag tccaccgaaa
     2281 aacccttaat cctgtcttca atgagcaatt tactttcaag gtaccatact cggaattggg
     2341 tggcaaaacc ctagtgatgg ctgtatatga ttttgatcgt ttctctaagc atgacatcat
     2401 tggagaattt aaagtcccta tgaacacagt ggattttggc catgtaactg aggaatggcg
     2461 tgacctgcaa agtgctgaga aggaagagca agagaaattg ggtgatatct gcttctccct
     2521 tcgctacgta cctactgctg gtaagctgac tgttgtcatt ctggaggcaa agaacctgaa
     2581 gaagatggat gtgggtggct tatccgatcc ttatgtgaag attcatctga tgcagaatgg
     2641 taagaggctg aagaagaaaa agacaacaat taaaaagaac acacttaacc cctactacaa
     2701 tgagtcattc agctttgaag taccttttga acaaatccag aaagtgcagg tggtggtaac
     2761 tgttttggac tatgacaaga ctggcaagaa cgatgccatc ggcaaagtct ttgtgggcta
     2821 caacagcacc ggcgcggagc tgcgacactg gtcagacatg ctggccaacc ccaggcgacc
     2881 tattgcccag tggcacaccc tgcaggtaga ggaggaagtt gatgccatgc tggccgtcaa
     2941 gaagtaggaa ttccgtacaa agcttatcga taatcaacct ctggattaca aaatttgtga
     3001 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     3061 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     3121 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     3181 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     3241 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     3301 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     3361 ggggaaatca tcgtcctttc cttggctgct cgcctatgtt gccacctgga ttctgcgcgg
     3421 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     3481 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     3541 cctttgggcc gcctccccgc atcgataccg gctcgagctg tgccttctag ttgccagcca
     3601 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     3661 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     3721 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     3781 ggggatgcgg tctcgagctg attttgtagg taaccacgtg cggaccgagc ggccgcagga
     3841 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
     3901 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     3961 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     4021 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
     4081 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct
     4141 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4201 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4261 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4321 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4381 aactctatct cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg
     4441 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     4501 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     4561 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     4621 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     4681 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     4741 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4801 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4861 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4921 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4981 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5041 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5101 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5161 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5221 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5281 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5341 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5401 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5461 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5521 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5581 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5641 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5701 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5761 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5821 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5881 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5941 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6001 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6061 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6121 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6181 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6241 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6301 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6361 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6421 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6481 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6541 ttcctggcct tttgctggcc ttttgctcac atgt
//