pCMV-dCpf1-eBE

LOCUS       pCMV-dCpf1-eBE                9133 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses dLbCpf1-eBE in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-dCpf1-eBE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Li X, Wang Y, Liu Y, Yang B, Wang X, Wei J, Lu Z, Zhang Y, Wu J, 
            Huang X, Yang L, Chen J
  TITLE     Base editing with a Cpf1-cytidine deaminase fusion.
  JOURNAL   Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102.
  PUBMED    29553573
REFERENCE   2  (bases 1 to 9133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9133
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        28..407
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..611
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     562..582
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     653..672
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        653..671
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             688..708
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             709..1395
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             5134..5154
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5167..5415
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5428..5448
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5455..5508
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5509..5757
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5758..5778
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5779..5835
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5836..6084
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6085..6105
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6106..6159
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6160..6408
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6421..6441
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6464..6481)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6470..6694
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6765..6781)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6765..6781)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6778..6800)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6789..6805
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6813..6843)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6858..6879
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6996..7013)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7167..7755)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7247..7266)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7926..8786)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8549..8568
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8787..8891)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8970..8989)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
       61 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      121 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      181 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      241 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      301 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      361 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      421 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      481 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      541 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      601 taagcagagc tggtttagtg aaccgtcaga tccgctagag atccgcggcc gctaatacga
      661 ctcactatag ggagagccgc caccatgccc aagaagaaga ggaaagtcat gagctcagag
      721 actggcccag tggctgtgga ccccacattg agacggcgga tcgagcccca tgagtttgag
      781 gtattcttcg atccgagaga gctccgcaag gagacctgcc tgctttacga aattaattgg
      841 gggggccggc actccatttg gcgacataca tcacagaaca ctaacaagca cgtcgaagtc
      901 aacttcatcg agaagttcac gacagaaaga tatttctgtc cgaacacaag gtgcagcatt
      961 acctggtttc tcagctggag cccatgcggc gaatgtagta gggccatcac tgaattcctg
     1021 tcaaggtatc cccacgtcac tctgtttatt tacatcgcaa ggctgtacca ccacgctgac
     1081 ccccgcaatc gacaaggcct gcgggatttg atctcttcag gtgtgactat ccaaattatg
     1141 actgagcagg agtcaggata ctgctggaga aactttgtga attatagccc gagtaatgaa
     1201 gcccactggc ctaggtatcc ccatctgtgg gtacgactgt acgttcttga actgtactgc
     1261 atcatactgg gcctgcctcc ttgtctcaac attctgagaa ggaagcagcc acagctgaca
     1321 ttctttacca tcgctcttca gtcttgtcat taccagcgac tgcccccaca cattctctgg
     1381 gccaccgggt tgaaaagcgg cagcgagact cccgggacct cagagtccgc cacacccgaa
     1441 agtatgtcaa agctggagaa attcaccaac tgttatagcc tgtctaagac cctgcgcttc
     1501 aaggcaatcc cagtgggcaa gacacaagag aacattgaca acaaacggct cctggtggag
     1561 gatgagaaga gggctgaaga ttacaagggc gttaagaagc tgctggatag gtactatctg
     1621 tcattcatca acgatgtcct ccacagtatc aagctgaaga atctgaacaa ttacatttct
     1681 ctgttccgga agaagacacg gaccgagaag gagaacaaag agctggagaa tctggagatc
     1741 aacctgagga aagaaatagc taaggctttc aaagggaacg agggttacaa gtccctgttc
     1801 aagaaagaca ttatcgagac tattctgcct gagttcctgg acgataaaga tgagatcgcc
     1861 ctcgtcaatt ccttcaatgg gtttaccaca gcctttaccg gcttcttcga caatagagag
     1921 aatatgttct ctgaagaggc caaatccact agcatcgcct ttcgctgcat aaacgagaac
     1981 ctgactaggt acatcagcaa tatggacatc tttgagaaag tcgatgccat attcgacaaa
     2041 catgaggtgc aggagattaa ggagaagatc ctgaactcag attacgatgt cgaagatttc
     2101 ttcgagggag agttcttcaa cttcgtgctc acacaagagg gcattgatgt gtacaatgca
     2161 atcattggag ggttcgtgac agagagtggc gagaagataa agggcctgaa cgagtatatc
     2221 aacctctaca accagaaaac caagcagaaa ctgcctaagt tcaagccact gtacaaacaa
     2281 gtgctctcag atagggaaag cctgagcttc tacggtgaag ggtatacatc agatgaagaa
     2341 gtgctcgaag tgttccgcaa caccctcaat aagaacagtg aaatcttctc ttcaatcaag
     2401 aagctggaga aactgttcaa gaatttcgat gagtactcct ctgccggaat ctttgtgaag
     2461 aatggccctg caatatccac tattagcaaa gacatctttg gcgagtggaa cgttatcagg
     2521 gataagtgga atgccgagta cgatgatatt catctcaaga agaaagccgt ggttacagag
     2581 aaatacgagg atgatagacg caagagcttt aagaagattg gtagcttctc tctcgaacag
     2641 ctgcaggagt acgccgacgc tgacctgtca gtcgtggaga aactcaagga gatcataatc
     2701 cagaaggtgg atgaaatcta caaagtgtat ggaagctctg agaaactctt cgatgcagac
     2761 tttgttctgg agaagagtct gaagaagaac gacgcagtgg ttgctatcat gaaggacctg
     2821 ctggattctg ttaagtcttt cgagaattac attaaggcat tctttggtga agggaaggag
     2881 acaaataggg acgagagctt ctatggcgac tttgttctgg cctacgacat cctcctcaag
     2941 gttgaccaca tctatgacgc tatacggaat tacgttaccc agaagcccta tagcaaagac
     3001 aagttcaagc tgtatttcca gaatccacag tttatgggtg ggtgggataa agacaaagaa
     3061 acagattaca gggccactat cctgcggtac ggcagcaaat actatctggc tatcatggat
     3121 aagaagtacg ccaaatgcct ccagaagatc gacaaggacg acgtgaacgg taactacgag
     3181 aagatcaatt acaagctcct gccaggacct aacaagatgc tgcccaaggt gttcttctcc
     3241 aagaaatgga tggcctacta taacccaagc gaggacattc agaagatata caagaatggg
     3301 acattcaaga agggcgatat gttcaacctc aacgactgcc acaagctgat tgatttcttc
     3361 aaggatagca tttctcgcta tcccaagtgg tctaatgcat acgatttcaa cttcagcgag
     3421 actgagaagt acaaagacat cgctggcttc taccgggagg tggaagagca aggctataag
     3481 gtgtcattcg aatccgcttc taagaaggaa gtggataagc tcgtggaaga gggtaagctg
     3541 tacatgttcc agatatacaa caaagacttc agcgataaga gccacggcac tccaaacctc
     3601 catactatgt atttcaagct gctgtttgac gagaacaacc acggacagat taggctgtca
     3661 ggaggcgcag aactcttcat gcgcagagct tcactgaaga aggaggaact cgttgtccac
     3721 ccagccaata gccctatagc caataagaat ccagacaatc ctaagaaaac cactactctg
     3781 tcttacgatg tgtataagga taagagattc tctgaagatc agtacgaact gcacataccc
     3841 attgccatta acaagtgccc taagaacatc ttcaagatta acacagaggt tagagtgctc
     3901 ctgaaacacg acgataaccc ttatgttata ggcattgctc gcggagagag aaacctgctg
     3961 tacatcgtcg tggtggacgg caaaggcaac atcgtggaac agtacagtct caatgaaatc
     4021 attaacaatt tcaacggaat ccgcattaag accgactacc attctctcct cgacaagaag
     4081 gagaaagaaa ggttcgaagc aagacagaat tggacaagta tagagaatat caaagaactg
     4141 aaggctgggt acatctctca ggttgtgcac aagatatgtg agctggtgga gaagtacgac
     4201 gctgttatcg ccctcgcgga cctgaatagc ggcttcaaga actccagggt gaaggtggag
     4261 aagcaggtgt atcagaagtt cgagaagatg ctgatcgaca agctcaacta tatggtggac
     4321 aagaaatcca atccttgcgc tactggtgga gccctgaagg gctatcaaat caccaataag
     4381 ttcgaatctt tcaagtctat gagcacccag aatggcttca tcttctacat acccgcatgg
     4441 ctgacatcca agattgatcc ctctaccgga tttgttaatc tgctcaagac taagtacacc
     4501 tctattgctg actcaaagaa gttcatatca tcatttgacc gcatcatgta cgtgccagaa
     4561 gaggacctgt tcgagtttgc cctggattac aagaatttct ctcggactga cgccgactac
     4621 atcaagaagt ggaagctcta ctcttatggt aatcggattc gcatattccg caatcccaag
     4681 aagaataacg tgttcgattg ggaggaagtt tgcctcacca gcgcttacaa ggagctgttc
     4741 aataagtatg ggattaacta ccagcagggc gacataagag ccctgctgtg cgaacaatct
     4801 gataaggcat tctattcctc tttcatggca ctgatgtcac tgatgctgca aatgcgcaat
     4861 tccatcaccg gaagaacaga cgtggccttt ctgatctctc ctgtcaagaa ctcagatggc
     4921 atcttctacg attcccgcaa ctatgaagca caggagaatg ctatcctgcc taagaatgcc
     4981 gatgcaaatg gagcctataa catcgccaga aaggtcctct gggccatagg acaattcaag
     5041 aaagctgaag atgagaagct ggacaaggtg aagatcgcca tttcaaacaa agagtggctc
     5101 gaatatgctc agacctcagt gaagcatgga tcacccaaga agaaacggaa agtgtctggt
     5161 ggttctacta atctgtcaga tattattgaa aaggagaccg gtaagcaact ggttatccag
     5221 gaatccatcc tcatgctccc agaggaggtg gaagaagtca ttgggaacaa gccggaaagc
     5281 gatatactcg tgcacaccgc ctacgacgag agcaccgacg agaatgtcat gcttctgact
     5341 agcgacgccc ctgaatacaa gccttgggct ctggtcatac aggatagcaa cggtgagaac
     5401 aagattaaga tgctctctgg tggttctccc aagaagaaga ggaaagtcgg gcccgagggc
     5461 agaggaagtc tgctaacatg cggtgacgtc gaggagaatc ctggcccaac taatctgtca
     5521 gatattattg aaaaggagac cggtaagcaa ctggttatcc aggaatccat cctcatgctc
     5581 ccagaggagg tggaagaagt cattgggaac aagccggaaa gcgatatact cgtgcacacc
     5641 gcctacgacg agagcaccga cgagaatgtc atgcttctga ctagcgacgc ccctgaatac
     5701 aagccttggg ctctggtcat acaggatagc aacggtgaga acaagattaa gatgctcccc
     5761 aagaagaaga ggaaagtcgc tactaacttc agcctgctga agcaggctgg agacgtggag
     5821 gagaaccctg gacctaccaa cctgtccgat atcattgaga aagagaccgg caaacagctg
     5881 gtgatccagg agagcatcct gatgctgccc gaagaggtgg aggaagtgat cggcaacaag
     5941 cccgagtccg acatcctggt gcacacagcc tatgatgaat ccaccgacga gaacgtgatg
     6001 ctgctgacct ccgatgctcc cgagtataaa ccctgggcac tggtgatcca ggactctaat
     6061 ggagagaaca agatcaagat gctgcccaag aagaagagga aagtcgaagg acggggctcc
     6121 ctcctgacct gtggcgatgt ggaagagaac cccggcccca caaacctcag tgacattatc
     6181 gagaaggaaa caggaaaaca gctcgtcatt caagaatcta ttcttatgtt gcctgaggaa
     6241 gtcgaagagg ttattggcaa taaacctgaa tctgatattc ttgtccatac cgcttacgat
     6301 gagtccacag atgaaaatgt tatgctgctc acatctgacg caccagagta caaaccatgg
     6361 gcgctcgtta ttcaagattc caacggcgaa aacaaaatca aaatgctttc tggtggttct
     6421 cctaaaaaaa aaagaaaggt gtgagtttaa acccgctgat cagcctcgac tgtgccttct
     6481 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     6541 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     6601 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     6661 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     6721 ggctcgatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     6781 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6841 aaagcctagg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6901 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     6961 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7021 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7081 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7141 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7201 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7261 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7321 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7381 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7441 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7501 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7561 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     7621 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7681 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7741 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7801 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7861 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7921 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7981 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8041 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8101 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8161 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8221 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8281 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8341 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8401 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8461 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8521 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8581 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8641 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8701 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8761 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8821 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8881 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcga
     8941 tctcccgatc ccctagggtc gactctcagt acaatctgct ctgatgccgc atagttaagc
     9001 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     9061 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     9121 tttgcgctgc ttc
//