| 质粒名称: | pHybrid-RGEPO1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000768 |
| 产品用途: | Expressing the red potassium indicator in the cytoplasm of mammalian cells or E.coli |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pHybrid |
| 表达系统: | 哺乳动物表达、细菌表达 |
| 启动子: | rhaB, CMV, SV40 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | Asp-RGEPO1, mScarlet |
| 物种来源: | cbacterium |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Sensitive red fluorescent indicators for real-time visualization of potassium ion dynamics in vivo.
Yang L., Pathiranage V., Zhou S., Sun X., Zhang H., Lai C., Gu C., Subach F.V., Drobizhev M., Walker A.R., Piatkevich K.D.
PLOS Biol. 23, e3002993 (2025)
|
买家导航
pHybrid-RGEPO1载体质粒基本信息
pHybrid-RGEPO1质粒图谱载体图谱和pHybrid-RGEPO1载体序列质粒序列多克隆位点信息
pHybrid-RGEPO1质粒载体简介
pHybrid-RGEPO1质粒序列载体序列
LOCUS pHybrid-RGEPO1 6053 bp DNA circular SYN 18-JUN-2025
DEFINITION TSN20241110-0571-00010_768_20241111-BAN3_B01_B01_i22.1 (6053 bp).
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6053)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 15, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6053
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 197..400
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 433..551
/label=rhaB promoter
/note="promoter of the E. coli rhaBAD operon, conferring
tight induction with L-rhamnose and repression with
D-glucose in the presence of RhaR and RhaS (Giacalone et
al., 2006)"
CDS 592..609
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
regulatory 640..649
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 646..1098
/codon_start=1
/label=N-mApple
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
FQTARLKVTKGGPLPFAWDILSPQFMYGSKAYIKHPADIPDYFKLSFPEGFRWERVMSF
EDGGIIHVNQDSSLQDGVFIYKVKLLGTNFPPDGPVMQKKTMGWEAS"
CDS 1102..1533
/codon_start=1
/label=YgaU_Hydrothermal
/translation="GIWDFVKSAGRKIGIGSEEAAPAADDLKKELDSYKLGTDDVKVEV
VGDKAVITGNVADQSILEKAIVAVGNTLGISKVESNVEVPDAKEPVFHTVKKGDTLWAI
ASKQYGNVSKYNAIFEANKPMLSHPDKIYPGQVLRIPDLG"
CDS 1543..1800
/codon_start=1
/label=C-mApple
/translation="VSEWMYPEDGALKSEIRKGLRLKDGGHYAAEVKTTYKAKKPVQLP
GAYIVDIELDILSHNEDYTIVEQCERAVGRHPTGGTDALYK"
terminator 1824..1910
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
polyA_signal 1934..2055
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 2096..2306
/label=SV40 promoter
/note="SV40 early promoter"
promoter 2118..2314
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 2165..2300
/label=SV40 ori
/note="SV40 origin of replication"
regulatory 2315..2324
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 2321..3016
/codon_start=1
/product="bright monomeric red fluorescent protein evolved
from a synthetic template (Bindels et al., 2016)"
/label=mScarlet-I
/note="matures faster than mScarlet"
/translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
STGGMDELYK"
polyA_signal 3021..3068
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 3148..3603
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3935..4039
/gene="bla"
/label=AmpR promoter
CDS 4040..4900
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5071..5659
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
enhancer join(5870..6053,1..196)
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
61 gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
121 ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt
181 catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt
241 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
301 ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga cgcaaatggg
361 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat
421 cactagaagc ttcaccacaa ttcagcaaat tgtgaacatc atcacgttca tctttccctg
481 gttgccaatg gcccattttc ctgtcagtaa cgagaaggtc gcgaattcag gcgcttttta
541 gactggtcgt aacccgtttt tggctaacag gaggaattaa ccatggggag ccaccatcac
601 catcaccatg ctagcctcga gaattcacgc gtggtaccag ccaccatggt gagcaagggc
661 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
721 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgaggcc
781 ttccagaccg ctaggctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
841 ctgtcccctc agttcatgta cggctccaag gcctacatta agcacccagc cgacatcccc
901 gactacttca agctgtcctt ccccgagggc ttcaggtggg agcgcgtgat gagcttcgag
961 gacggcggca ttattcacgt taatcaggac tcctccctgc aggacggcgt attcatctac
1021 aaggtgaagc tgctcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc
1081 atgggctggg aggcttcccc cggcatctgg gacttcgtga agagcgccgg caggaagatc
1141 ggcatcggca gcgaggaggc cgcccccgcc gccgacgacc tgaagaagga gctggacagc
1201 tacaagctgg gcaccgacga cgtgaaggtg gaggtggtgg gcgacaaggc cgtgatcacc
1261 ggcaacgtgg ccgaccagag catcctggag aaggccatcg tggccgtggg caacaccctg
1321 ggcatcagca aggtggagag caacgtggag gtgcccgacg ccaaggagcc cgtgttccac
1381 accgtgaaga agggcgacac cctgtgggcc atcgccagca agcagtacgg caacgtcagc
1441 aagtacaacg ccatcttcga ggccaacaag cccatgctga gccaccccga caagatctac
1501 cccggccagg tgctgaggat ccccgacctg ggctctgcct gcgtttccga gtggatgtac
1561 cccgaggacg gcgccctgaa gagcgagatc aggaaggggc tgaggctgaa ggacggcggc
1621 cactacgccg ccgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
1681 gcctacatcg tcgacatcga gttggacatc ttgtcccaca acgaggacta caccatcgtg
1741 gaacagtgcg aacgcgccgt gggccgccac cccaccggtg gcacggacgc gctgtacaag
1801 tagtctagag tcgacccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
1861 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat gcggccgctt
1921 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
1981 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
2041 tgcaataaac aagttaacaa caacaattgg gtgtggaaag tccccaggct ccccagcagg
2101 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
2161 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
2221 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
2281 aggaggcttt tttggaggcc taggcttttg caaagccacc atggtgagca agggcgaggc
2341 agtgatcaag gagttcatgc ggttcaaggt gcacatggag ggctccatga acggccacga
2401 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
2461 gaaggtgacc aagggtggcc ccctgccctt ctcctgggac atcctgtccc ctcagttcat
2521 gtacggctcc agggccttca tcaagcaccc cgccgacatc cccgactact ataagcagtc
2581 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgccgtgac
2641 cgtgacccag gacacctccc tggaggacgg caccctgatc tacaaggtga agctccgcgg
2701 caccaacttc cctcctgacg gccccgtaat gcagaagaag acaatgggct gggaagcgtc
2761 caccgagcgg ttgtaccccg aggacggcgt gctgaagggc gacattaaga tggccctgcg
2821 cctgaaggac ggcggccgct acctggcgga cttcaagacc acctacaagg ccaagaagcc
2881 cgtgcagatg cccggcgcct acaacgtcga ccgcaagttg gacatcacct cccacaacga
2941 ggactacacc gtggtggaac agtacgaacg ctccgagggc cgccactcca ccggcggcat
3001 ggacgagctg tacaagtgac cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
3061 gtttgttcgg atccgggctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
3121 cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg
3181 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
3241 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
3301 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
3361 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
3421 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
3481 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
3541 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
3601 tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
3661 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
3721 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
3781 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
3841 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
3901 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
3961 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
4021 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
4081 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
4141 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
4201 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
4261 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
4321 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
4381 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
4441 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
4501 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
4561 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
4621 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
4681 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
4741 agccggtgag cgtggctctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
4801 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
4861 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
4921 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
4981 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
5041 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
5101 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
5161 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
5221 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
5281 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
5341 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
5401 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
5461 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
5521 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
5581 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
5641 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
5701 ctggcctttt gctcacatgg ctcgacagat cttcaatatt ggccattagc catattattc
5761 attggttata tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat
5821 cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgttg gcattgatta
5881 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
5941 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
6001 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac ttt
//
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