pPuro3.1(+)_Strep-Lrrk2(D1994S)-Myc

LOCUS       pPuro3.1(+)_Strep-Lrrk2(D1994S)-Myc               12584 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  expression of Myc-tagged kinase-dead mouse Lrrk2 containing D1994S 
            mutation.
ACCESSION   .
VERSION     .
KEYWORDS    pPuro3.1(+)_Strep-Lrrk2(D1994S)-Myc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12584)
  TITLE     Lrrk2 expression plasmids
REFERENCE   2  (bases 1 to 12584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..12584
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      665..674
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             680..703
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     RBS             1842..1850
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             8303..8332
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(8361..8378)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8367..8591
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8724..8743)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(8767..8787)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8772..9101
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8952..9087
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9014..9033
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9194..9793
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9194..9213)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9690..9710
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    10023..10144
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10060..10079)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10114..10133
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10193..10209)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10193..10209)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10206..10228)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10217..10233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10241..10271)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10286..10307
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10424..10441)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10595..11183)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10675..10694)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11354..12214)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11977..11996
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12215..12319)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12394..12413)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagccacc atggcaagct ggagccaccc gcagttcgaa aaagccggca tggccagtgg
      721 cgcctgtcag ggctgcgaag aggaagagga ggaggaggct ctgaagaagt tgatagtcag
      781 gctgaataat gtccaggaag gcaagcagat cgagacgttg cttcagctcc tggaggacat
      841 gctggtgttc acctactcgg accgcgcctc caagttattt gaagataaaa atttccacgt
      901 gcctctgttg attgtcctgg actcctacat gagagttgcc agtgtacagc aggcggggtg
      961 gtcacttctg tgcaaattaa tagaagtctg tccagggaca ttgcaaagct taataggacc
     1021 ccaggatatt ggaaatgatt gggaagtcct tggtattcac cggctgattc ttaaaatgtt
     1081 aactgttcat cacgccaatg taaacctgtc aatagttgga ctaaaagcct tggatctcct
     1141 cctagattca ggtaaactca ccttgctgat actggatgaa gaatgtgata ttttcttgtt
     1201 aatttttgat gccatgcaca gatattcagc caatgatgaa gtccaaaaac tgggatgcaa
     1261 agctttacac gtgctttttg agagagtttc cgaggaacag ctgactgagt ttgtggagaa
     1321 caaagattac acgatactgc tgagtacgtt cggcagcttc agaagggaca aggagattgt
     1381 gtaccacgta ctttgctgct tgcattccct ggcggttaca tgcagcaatg tagaggtcct
     1441 catgagtggg aatgtccggt gctacaatct tgtggtggag gccatgaaag ccttccccac
     1501 caatgaaaac atccaagagg tgagctgctc cttgttccag aagcttacat taggtaactt
     1561 tttcaacatc ctggtgttga acgaagtgca tgtctttgtg gtgaaagcgg tccgacagta
     1621 tcctgagaac gcagccttac agatctctgc actcagctgt ttagcactcc tcactgagac
     1681 tattttctta aaccaagact tggaggaaag aagtgagact caagagcaga gcgaagagga
     1741 agacagtgag aagcttttct ggctggaacc ctgctataaa gccctggtgc gccatcgaaa
     1801 ggacaaacac gtgcaggagg ctgcctgctg ggcactaaat aacctcctta tgtaccagaa
     1861 cagtttgcat gagaagatcg gagatgaaga tggccagttc cctgcgcaca gggaagtgat
     1921 gctgtctatg ctgatgcact cttcttccaa agatgtcttc caagcagctg cacatgctct
     1981 gtccactctc ttggaacaaa atgttaattt caggaaaatc ctgctggcaa aaggagtata
     2041 cctgaatgtc ttggaattga tgcagaagca tgcccatgcg cctgaggtgg cagagagtgg
     2101 ctgcaagatg ctgagtcacc tgtttgaagg aagtaaccct tctctggata caatggcagc
     2161 agtggtccct aaaatactaa cagtgatgaa agcccacgga acgtctctgt cagtccagct
     2221 ggaggcgctg cgagctatct tgcatttcgt tgtgccagga ctattggaag aatccaggga
     2281 ggactctcaa tgcagaccaa atgtgctcag aaaacagtgt ttcaggactg acatccacaa
     2341 gctggttcta gtcgctctga acaggttcat tgggaatcct gggattcaga aatgtggatt
     2401 gaaagtaatc tcttctctcg cgcaccttcc tgatgccaca gagacattgt ccctgcaagg
     2461 agcagttgac tcagtcctcc acaccttaca gatgtatcca gatgaccaag aaattcagtg
     2521 tctgggctta caccttatgg gatgcttgat gacaaagaag aatttctgca tagggacagg
     2581 gcacctcctg gcaaaaattc tggcttccac tttgcagcgc tttaaagatg ttgctgaggt
     2641 gcagactaca ggattacaga caaccctgtc aatacttgag ctgtcagtat ctttctccaa
     2701 gctgctagtg cactattcct ttgatgtggt gatatttcat cagatgtctt ccagtgttgt
     2761 agaacaaaag gatgagcagt tcctcaatct atgttgcaaa tgctttgcaa aagtggccgt
     2821 ggatgatgag ctgaaaaaca ccatgctaga gagagcctgc gatcagaata acagcatcat
     2881 ggttgaatgt ttgctcctct tgggagctga tgccaaccaa gtgaaggggg caacttcttt
     2941 aatctatcag gtatgtgaga aagagagcag tcctaaattg gtggaactgt tgcttaatgg
     3001 tggttgtcgt gaacaagatg tacggaaggc cctgaccgta agcatccaaa agggcgacag
     3061 ccaggtcatc agcttgctcc tcaggaaact tgccctggac ctggccaaca acagcatttg
     3121 ccttggagga tttggcatag gaaaaattga tccttcttgg cttggtcctt tatttccaga
     3181 taagtcatcc aatttaagga agcaaacaaa cacaggatct gtcctagcga ggaaagtgct
     3241 ccggtatcag atgagaaaca cccttcaaga aggcgtggcc tcaggcagtg acggcaagtt
     3301 ttctgaagac gcgctggcga aatttggaga atggaccttt attcccgact cttctatgga
     3361 cagtgtgttt ggccagagcg atgatctgga tagcgaaggc agcgagagct catttctcgt
     3421 gaagaggaag tccaactcaa ttagtgtagg ggaagtttac agagatctag ctctgcagcg
     3481 ctgctcacca aatgctcaga ggcattccaa ttcgctgggt cctgtttttg accatgaaga
     3541 cttactgaga cgaaaaagaa aaatactgtc ttcagatgag tctctcaggt cctcaaggct
     3601 gccgtcccat atgaggcaat cagatagctc ttcttccctg gcttctgaga gagaacacat
     3661 cacgtcgtta gacctatctg ccaacgaact caaagatatt gatgctctga gccagaagtg
     3721 ttgcctcagt agccacctgg aacatctcac caaactggaa cttcaccaga attcactcac
     3781 gagcttccca cagcagctgt gtgagactct gaagtgtttg atacacttgg atttgcacag
     3841 taacaaattc acctcatttc cctctttcgt gttgaaaatg ccacgtatca ccaacctaga
     3901 tgcctctcga aatgacatcg ggccaacagt agttttagac cctgcgatga agtgtccaag
     3961 cctcaaacag ttgaatctgt cctataacca gctctcttca atcccagaga atcttgccca
     4021 agtggtggag aaacttgagc agctcctact ggaaggaaat aaaatatccg ggatttgctc
     4081 tcccctgagc ctgaaggaac tgaagatttt aaatcttagt aaaaatcaca ttccatccct
     4141 acctggagat tttcttgagg cttgttcaaa agtcgagagt ttcagtgctc gcatgaattt
     4201 tcttgctgca atgcctgcct taccttcttc cataacgagc ttaaaattgt ctcagaactc
     4261 tttcacgtgc attccagaag cgattttcag tcttccgcac ttgcggtcct tggatatgag
     4321 ccacaacaac attgaatgtc tgccgggacc tgcacattgg aagtctctga acttaaggga
     4381 actcattttt agcaagaatc agatcagcac cttagacttt agtgagaacc cacacgtgtg
     4441 gtcaagagta gagaaactgc atctctctca taataaactg aaagagattc ctccagaaat
     4501 tggctgcctt gaaaatctga cgtctctcga cgtcagttac aacttggaac tgaggtcctt
     4561 cccaaatgaa atggggaagt tgagcaagat atgggatctt cccttggacg gactgcatct
     4621 gaattttgac tttaagcacg taggatgcaa ggccaaagac atcataaggt ttctacaaca
     4681 acgtctgaaa aaggctgtac cctacaaccg aatgaagctc atgattgtgg gaaatacggg
     4741 gagcggtaag accactttac tgcaacaact catgaaaatg aagaaaccag aacttggcat
     4801 gcagggtgcc acagtcggca tagacgtgcg agactggtcc atccaaatac ggggcaaaag
     4861 gagaaaggac ctggttctaa acgtgtggga ttttgcaggt cgtgaggaat tctacagcac
     4921 tcacccccac ttcatgaccc agagagccct ctacctggct gtctatgatc tcagcaaggg
     4981 gcaggcagag gtggacgcca tgaagccctg gctcttcaat atcaaggctc gtgcctcttc
     5041 ttccccggtg attctggtgg gcacacattt ggatgtttct gatgagaagc agcggaaagc
     5101 gtgcataagc aaaatcacga aggaactcct aaataagcga ggattcccca ccatccggga
     5161 ctaccacttt gtgaatgcca ccgaggagtc agatgcgctg gcaaagcttc ggaaaaccat
     5221 cataaatgag agccttaatt tcaagatccg agatcagcct gtggttgggc agctaattcc
     5281 agattgctac gtagaactgg agaaaatcat tttatcagag cggaaagctg tgccgactga
     5341 gtttcctgtg attaaccgga aacacctgtt acagctcgtg aacgaacatc agctgcagct
     5401 ggatgagaac gagctcccac acgccgttca cttcctaaat gagtcgggag ttcttctgca
     5461 ttttcaagac cctgccctgc agctaagtga cctgtacttt gtggaaccca agtggctttg
     5521 taaagtcatg gcacagatct tgacagtgaa ggtagacggc tgtctgaaac atcctaaggg
     5581 catcatttcc cggagagatg tggaaaaatt cctttcaaag aagaagcgat tcccgaagaa
     5641 ctatatgatg caatacttta aactattaga aaaatttcag atcgcattgc caatagggga
     5701 agaatatctt ctggttccaa gcagcttgtc tgaccacagg ccagtgatag agctccccca
     5761 ctgtgagaac tctgagatca tcatccggct gtacgaaatg ccgtactttc ccatgggatt
     5821 ttggtcaaga ttgattaacc gattacttga aatctcaccc ttcatgcttt ctggcagaga
     5881 gagagcacta cgccctaaca ggatgtattg gcggcaaggc atctacttga attggtctcc
     5941 agaagcatac tgtctggtag gctctgaagt cttagacaat cgacctgaga gtttcttgaa
     6001 aatcacagtt ccgtcttgta gaaaaggttg tattcttctg ggccgagttg tggatcatat
     6061 tgactcactc atggaagaat ggtttcccgg gttactggag attgacattt gtggggaagg
     6121 agaaactctg ttgaagaaat gggcattgta cagttttaat gatggtgaag aacatcagaa
     6181 gatcttgctt gatgagttga tgaagaaggc tgaagaagga gacctgttaa taaatccaga
     6241 ccaaccaagg ctcactattc caatatccca gattgctccg gacttgatct tggctgacct
     6301 gcctagaaat atcatgttga acaatgatga gttggaattt gaggaagcac cagagtttct
     6361 cttaggcgat ggaagttttg gatccgttta tcgagctgcc tacgaaggag aggaagtggc
     6421 tgtgaagatt tttaataagc acacatctct taggctgtta agacaagagt tggtggtcct
     6481 ttgtcacctt caccacccca gcctgatatc cttgctggcg gctggtattc gtcctcggat
     6541 gttggtaatg gagttggcct ccaaaggttc cttggatcgc ctgctgcagc aggacaaagc
     6601 cagcctcacc agaaccctcc agcacaggat cgcgttgcat gtggccgacg gcctgaggta
     6661 tctccactca gccatgatta tttaccgttc cctgaagccc cacaatgtgc tgctttttac
     6721 cctgtatccc aatgctgcca tcattgcgaa gattgcggac tacgggatcg cacagtactg
     6781 ctgcaggatg ggaataaaga catcagaggg caccccaggg ttccgggcac ctgaagttgc
     6841 cagggggaat gtcatttata accaacaggc cgatgtttat tcttttggct tactacttca
     6901 cgatatttgg acaactggga gtaggattat ggagggtttg aggttcccaa atgagtttga
     6961 tgagttagcc atacaaggga agttgccaga tccagttaaa gaatatggct gtgccccatg
     7021 gcctatggtt gagaagttaa ttacaaagtg tttgaaagaa aatcctcaag aaagacccac
     7081 ttctgcccag gtctttgaca ttttgaattc ggctgaatta atttgcctca tgcgacacat
     7141 tttaatacct aagaacatca ttgttgaatg catggttgcc acgaatctca atagcaagag
     7201 tgcgactctc tggttgggat gtgggaacac agaaaaagga cagctttcct tatttgactt
     7261 aaacacggaa agatacagct atgaggaagt tgctgatagt agaatactgt gcttggcttt
     7321 ggtgcatctc gctgctgaga aagagagctg ggttgtgtgc gggacacagt ctggggctct
     7381 cctggtcatc aatgttgaag aggagacaaa gagacacacc ctggaaaaga tgactgattc
     7441 tgtcacttgt ttgcattgca attcccttgc caagcagagc aagcaaagta actttctttt
     7501 ggtgggaact gctgatggta acttaatgat atttgaagat aaagccgtta agtgtaaagg
     7561 agctgccccc ttgaagacac tacacatagg cgatgtcagt acgcccctga tgtgcctgag
     7621 cgagtccctg aattcatctg aaagacacat cacatgggga gggtgtggca caaaggtctt
     7681 ctccttttcc aatgatttca ccattcagaa actcatcgag acaaaaacca accagctgtt
     7741 ttcttacgca gctttcagcg attctaacat catagcgctg gcagtagaca cagccctgta
     7801 tattgccaag aaaaacagcc ctgtcgtaga ggtgtgggac aagaaaacag aaaagctctg
     7861 tgaattaata gactgtgtgc acttcttaaa ggaggtgatg gtaaaactaa acaaggaatc
     7921 gaaacatcag ctgtcctact ctgggagggt gaaggccctc tgcctgcaga agaacacggc
     7981 tctctggatc ggaactggag gaggccacat cttactcctg gatctttcta ctcggcgagt
     8041 tatccgcacc attcacaatt tctgtgattc tgtgagagcc atggccacag cacaattagg
     8101 aagccttaag aatgtcatgc tggttttggg gtacaagcgg aagagtacag agggtatcca
     8161 agaacaaaaa gagatacaat cttgtttgtc tatttgggac ctcaatcttc cacacgaggt
     8221 gcaaaattta gaaaaacaca ttgaagtaag aacagaatta gctgataaaa tgaggaaaac
     8281 atctgttgaa ggtaccgccc tcgagcaaaa actcatctca gaagaggatc tgtaggggcc
     8341 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     8401 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     8461 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     8521 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     8581 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     8641 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     8701 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     8761 cgctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     8821 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     8881 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     8941 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     9001 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     9061 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgca tgcctgcagg
     9121 tcggccgcca cgaccggtgc cgccaccatc ccctgaccca cgcccctgac ccctcacaag
     9181 gagacgacct tccatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     9241 cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     9301 cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     9361 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     9421 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     9481 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     9541 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     9601 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     9661 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     9721 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     9781 gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc
     9841 ccatggctcc gaccgaagcc acccggggcg gccccgccga ccccgcaccc gcccccgagg
     9901 cccaccgact ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     9961 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
    10021 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    10081 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    10141 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
    10201 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    10261 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
    10321 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
    10381 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
    10441 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    10501 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    10561 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    10621 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    10681 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    10741 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    10801 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    10861 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    10921 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    10981 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
    11041 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    11101 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    11161 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    11221 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    11281 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    11341 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    11401 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    11461 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    11521 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    11581 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    11641 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    11701 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    11761 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    11821 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    11881 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    11941 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    12001 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    12061 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    12121 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    12181 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    12241 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    12301 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
    12361 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
    12421 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
    12481 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
    12541 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtt
//