| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TNRC6B-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001565 |
| 产品用途: | 3' AAVLINK plasmid for TNRC6B expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | TNRC6B |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TNRC6B-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TNRC6B-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TNRC6B-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TNRC6B-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TNRC6B-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TNRC6B-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TNRC6B-FLAG 7251 bp DNA circular SYN 12-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7251)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 18, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7251
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3964
/codon_start=1
/label=TNRC6B
/translation="GSASSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLL
SQTEDNPSSKMDLSVGSLSDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDS
GPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLPNVSLGAIGTGLNPQNF
AARQGGSHGLFGNSTAQSRGLHTPVQPLNSSPSLRAQVPPQFISPQVSASMLKQFPNSG
LSPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKISQAVRQQ
QEQQLARMVSALQQQQQQQQRQPGMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGP
IPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEANMHKNGAIVAPG
KTRGGSPYNQFDIIPGDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVP
WKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWP
YSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLPRPPP
GLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGS
TLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDD
EVSRFLAQAQPPTPAATPSAPAAGWQSLETGQNQSDPVGPALNLFGGSTGLGQWSSSAG
GSSGADLAGASLWGPPNYSSSLWGVPTVEDPHRMGSPVPLLPGDLLGGGSDSI"
misc_feature 1585..1590
/label=SDS
misc_feature 1618..1623
/label=SDS
misc_feature 2628..2633
/label=SDS
misc_feature 3269..3274
/label=SDS
misc_feature 3546..3551
/label=SDS
misc_feature 3946..3951
/label=SDS
CDS 3971..4036
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 4040..4471
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4184..4189
/label=SDS
repeat_region 4514..4654
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4514..4643
/label=AAV2 ITR
rep_origin 4729..5184
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5466..5570
/gene="bla"
/label=AmpR promoter
CDS 5571..6431
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6602..7190
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggca gtgcttcctc
1561 ccacaactca gcaagctggg gacaaggagg aaagaaacaa atgaagtgct cactcaaagg
1621 aggaaacaat gattcatgga tgaatcctct tgccaaacag ttttcaaata tgggattgct
1681 gagtcagact gaagataatc caagcagcaa aatggatttg tctgtaggaa gcctttcaga
1741 taaaaaattt gatgtggaca agcgagcgat gaatctcggg gattttaatg atatcatgag
1801 gaaggatcga tctgggttcc gtccacctaa ttccaaagac atgggaacca cagatagtgg
1861 gccttatttt gagaagctga ctttgccttt ctccaatcaa gatgggtgcc ttggggatga
1921 ggctccctgc tctcccttct ccccttctcc cagctacaag ctgtctccct ctggttccac
1981 actacccaac gtcagccttg gagcaatcgg cacagggctc aacccccaaa acttcgctgc
2041 tagacaaggc ggtagtcatg gtttgtttgg aaacagcaca gcacaatcga gaggtctgca
2101 cacacccgtg cagccactaa attcttctcc cagtctccgg gcgcaagtgc ctccccagtt
2161 tatttccccc caggtttctg cctcaatgct caagcagttt cccaacagtg gcctgagtcc
2221 aggtcttttc aatgtggggc cccagttatc tcctcaacaa attgccatgc tgagccagct
2281 tccacaaatt ccccagtttc agttggcatg tcagcttctc ttgcagcagc agcaacagca
2341 gcagttgtta cagaaccaga gaaagatttc tcaagctgta cgccaacagc aagagcagca
2401 gctggctcga atggtgagtg cactgcagca gcagcagcag cagcagcaga ggcagccagg
2461 catgaagcac tcgccctctc atcctgttgg gcccaagccg catctggaca acatggtacc
2521 caacgcattg aatgtggggc tcccagacct tcaaaccaaa gggccaatac ctggatatgg
2581 ttctggcttc agctctggcg gcatggacta tggcatggtt ggtgggaagg aggctggaac
2641 cgagtctcgc tttaaacagt ggacctccat gatggagggg ctgccctctg tagccacaca
2701 ggaagccaat atgcacaaaa atggcgctat agtggcccct ggtaaaaccc ggggagggtc
2761 accgtacaac cagtttgata tcatccctgg tgacacactg ggtggccata cgggtcctgc
2821 tggtgatagc tggttacctg ccaaatctcc accaacaaat aaaatcggaa gtaaatccag
2881 caatgccagt tggcctccag aattccaacc aggagtgcca tggaaaggta tccaaaacat
2941 tgaccctgaa tctgacccct atgtcacccc aggaagtgtg ctggggggta cagccacatc
3001 tcccattgta gatactgacc accaactgct gcgggataac accacagggt ctaattcttc
3061 cctcaacacc tcgctgcctt cacctggtgc ctggccctac agtgcctctg acaactcctt
3121 taccaacgtt catagcactt cagcaaagtt ccctgattac aaatcaacat ggtccccaga
3181 tcccatagga cacaacccca ctcatctctc caacaagatg tggaaaaacc atatttcctc
3241 caggaacact acaccgctgc cccgcccacc tcctggtctg accaacccca aaccatcatc
3301 tccctggagc agcacagcac cccgatcagt cagggggtgg gggacacagg actcacggct
3361 cgcctcggcc tctacctgga gtgatggtgg ctcagttcgt cctagttact ggctggttct
3421 tcacaatctc accccacaga ttgatgggtc aaccttgaga acgatctgca tgcagcatgg
3481 cccactgctg acattccatc tgaatctaac ccagggcact gccctgatcc gatacagcac
3541 caaacaggag gcggccaagg cccaaactgc actgcacatg tgtgtgttgg gaaacactac
3601 catccttgct gagtttgcca ctgatgatga agtcagccgc tttctggcac aagctcagcc
3661 ccctacacct gcagcaaccc caagtgcgcc agctgcgggg tggcagtcgc tggagaccgg
3721 ccagaaccag tcagatcccg tgggacctgc tctgaatctt tttggtgggt ccactgggct
3781 cgggcagtgg agcagcagcg ctggtggcag cagcggggcc gatcttgctg gcgcttcatt
3841 gtgggggccc ccaaactatt cttctagctt atggggagtc ccaacggtgg aagatcccca
3901 taggatgggc agccctgttc ctttactacc tggtgacctt ctgggaggag ggtcggattc
3961 aatctgtaca gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa
4021 ggatgacgat gacaagtaac tcgagataat caacctctgg attacaaaat ttgtgaaaga
4081 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
4141 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
4201 tggttagttc ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
4261 gctcggctgt tgggcactga caattccgtg gtgtttattt gtgaaatttg tgatgctatt
4321 gctttatttg taaccatcta gctttatttg tgaaatttgt gatgctattg ctttatttgt
4381 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
4441 ggttcagggg gagatgtggg aggtttttta aagatctgga gaccggtgtg gcacgtgcgg
4501 accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
4561 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
4621 tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt
4681 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
4741 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
4801 gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
4861 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
4921 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
4981 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
5041 aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc
5101 cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
5161 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
5221 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
5281 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
5341 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
5401 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
5461 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
5521 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
5581 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
5641 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
5701 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
5761 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
5821 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
5881 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
5941 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
6001 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
6061 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
6121 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
6181 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
6241 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
6301 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
6361 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
6421 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
6481 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
6541 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
6601 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
6661 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
6721 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
6781 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
6841 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
6901 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
6961 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
7021 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
7081 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
7141 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
7201 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
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pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TNRC6B-FLAG质粒载体应用举例
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