| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KAT6A-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001610 |
| 产品用途: | 3' AAVLINK plasmid for KAT6A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KAT6A |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KAT6A-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KAT6A-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KAT6A-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KAT6A-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KAT6A-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KAT6A-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KAT6A-FLAG 7173 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7173)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 20, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7173
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
misc_feature 1545..1550
/label=SDS
CDS 1547..3886
/codon_start=1
/label=KAT6A
/translation="EEEEMQAEAEEAEEGEEEDAASSEVPAASPADSSNSPETETKEPE
VEEEEEKPRVSEEQRQSEEEQQELEEPEPEEEEDAAAETAQNDDHDADDEDDGHLESTK
KKELEEQPTREDVKEEPGVQESFLDANMQKSREKIKDKEETELDSEEEQPSHDTSVVSE
QMAGSEDDHEEDSHTKEELIELKEEEEIPHSELDLETVQAVQSLTQEESSEHEGAYQDC
EETLAACQTLQSYTQADEDPQMSMVEDCHASEHNSPISSVQSHPSQSVRSVSSPNVPAL
ESGYTQISPEQGSLSAPSMQNMETSPMMDVPSVSDHSQQVVDSGFSDLGSIESTTENYE
NPSSYDSTMGGSICGNSSSQSSCSYGGLSSSSSLTQSSCVVTQQMASMGSSCSMMQQSS
VQPAANCSIKSPQSCVVERPPSNQQQQPPPPPPQQPQPPPPQPQPAPQPPPPQQQPQQQ
PQPQPQQPPPPPPPQQQPPLSQCSMNNSFTPAPMIMEIPESGSTGNISIYERIPGDFGA
GSYSQPSATFSLAKLQQLTNTIMDPHAMPYSHSPAVTSYATSVSLSNTGLAQLAPSHPL
AGTPQAQATMTPPPNLASTTMNLTSPLLQCNMSATNIGIPHTQRLQGQMPVKGHISIRS
KSAPLPSAAAHQQQLYGRSPSAVAMQAGPRALAVQRGMNMGVNLMPTPAYNVNSMNMNT
LNAMNSYRMTQPMMNSSYHSNPAYMNQTAQYPMQMQMGMMGSQAYTQQPMQPNPHGNMM
YTGPSHHSYMNAAGVPKQSLNGPYMRR"
misc_feature 1548..1553
/label=SDS
misc_feature 1551..1556
/label=SDS
misc_feature 1686..1691
/label=SDS
misc_feature 1734..1739
/label=SDS
misc_feature 1770..1775
/label=SDS
misc_feature 1773..1778
/label=SDS
misc_feature 1899..1904
/label=SDS
misc_feature 2106..2111
/label=SDS
CDS 3893..3958
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3962..4393
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4106..4111
/label=SDS
repeat_region 4436..4576
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4436..4565
/label=AAV2 ITR
rep_origin 4651..5106
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5388..5492
/gene="bla"
/label=AmpR promoter
CDS 5493..6353
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6524..7112
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggagg aggaggagat
1561 gcaagcagag gcagaagagg ctgaagaggg tgaggaagag gatgcagcca gcagtgaagt
1621 cccagcagcc tctccagcag acagcagcaa tagtcctgag accgaaacca aggagcctga
1681 ggtggaggag gaagaagaga agccccgtgt ctcagaggag cagaggcagt cagaggagga
1741 gcagcaggaa ttagaggagc cagagccaga ggaggaggaa gatgcagctg cagagactgc
1801 ccagaatgac gaccacgacg ctgatgatga ggatgatggc cacctggagt ccacaaagaa
1861 aaaggagcta gaggaacagc ccacgaggga agatgtcaag gaggagcctg gtgttcaaga
1921 gtctttttta gatgctaata tgcagaagag tagggaaaag ataaaggata aagaggaaac
1981 cgagctggat tccgaagagg agcagccttc ccatgacacg tccgtggtgt cagagcagat
2041 ggctgggtct gaggacgacc acgaagaaga ctcccacact aaggaagagt taatcgaatt
2101 aaaagaggag gaagagattc ctcatagtga gctggatctg gaaactgtac aggcagtgca
2161 gtctttgact caagaagaaa gcagtgagca tgagggcgcc taccaggact gtgaggaaac
2221 tcttgcggcg tgtcagaccc tgcagagtta cacccaggct gacgaggacc ctcagatgtc
2281 catggttgaa gactgtcatg cgtcagaaca taatagccct atctcctccg ttcagtctca
2341 ccccagccag tcagtccgtt cggtcagcag tcccaacgtg cctgcccttg agagtggcta
2401 cacccagatc agcccagaac aaggatccct gtccgcaccc tctatgcaga acatggagac
2461 cagccccatg atggatgtgc cttccgtatc agaccactct cagcaggtgg tggacagcgg
2521 cttcagtgac ctgggcagca ttgagagcac cactgaaaac tatgagaacc caagcagtta
2581 cgactccacg atgggcggca gcatctgtgg gaacagctct tcccagagca gctgctccta
2641 cggtgggctg tcgtcctcca gcagcctcac ccagagcagc tgtgtggtca ctcagcagat
2701 ggccagcatg ggcagcagct gcagcatgat gcagcagagc agcgtccagc ctgctgccaa
2761 ctgcagcatc aagtcacctc agagctgcgt ggtggagagg cctcccagta accagcagca
2821 gcagccgcca ccaccgcctc cacagcagcc acagccgccg ccgccacaac cacaaccagc
2881 accacagcct ccaccacccc agcagcagcc gcaacagcag ccgcagcctc agccccagca
2941 gcctccaccc ccaccccctc cccagcagca gcccccgctg tcacagtgta gtatgaataa
3001 cagtttcacc ccagctccta tgatcatgga gataccagaa tctggaagca ctgggaacat
3061 aagtatctat gagaggattc caggggattt tggtgccggc agctactctc aaccatcagc
3121 caccttcagc ctagccaagc tgcagcagct gaccaacacc attatggacc ctcatgccat
3181 gccttatagc cattctcctg ctgtgacttc ctatgcaacc agtgtttctc tgtccaatac
3241 aggactggct cagctggctc catctcatcc cttagctggg actcctcaag cacaagccac
3301 catgacgcca cccccaaact tggcatccac taccatgaac ctcacatctc ctctgcttca
3361 gtgcaacatg tctgccacca acattggcat tcctcacacg cagagattgc aagggcaaat
3421 gccagtgaag gggcacattt ccatccgctc caagtctgcg ccactgccct ctgcggctgc
3481 tcaccagcag cagctgtatg gccgtagccc atcggcagtt gccatgcagg ctggccctcg
3541 cgcactggct gttcagcgtg gcatgaacat gggggttaat ctgatgccta ctcccgccta
3601 taatgtcaat tccatgaata tgaacacctt gaatgccatg aacagctatc gaatgacaca
3661 gcccatgatg aacagcagtt accatagtaa ccctgcctac atgaaccaga cagcacagta
3721 tcctatgcag atgcagatgg gaatgatggg gagccaggcc tatacccagc agcctatgca
3781 gcctaaccct catgggaaca tgatgtacac aggcccctcc catcacagct acatgaacgc
3841 tgctggcgtg cccaagcagt cactcaacgg accttacatg agaagatgta cagactacaa
3901 agaccatgac ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta
3961 actcgagata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
4021 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
4081 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttagt tcttgccacg
4141 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
4201 gacaattccg tggtgtttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatc
4261 tagctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
4321 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
4381 ggaggttttt taaagatctg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa
4441 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
4501 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
4561 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
4621 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
4681 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
4741 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
4801 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
4861 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
4921 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
4981 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
5041 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
5101 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
5161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
5221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
5281 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
5341 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
5401 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
5461 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
5521 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
5581 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
5641 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
5701 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
5761 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
5821 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
5881 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
5941 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
6001 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
6061 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
6121 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
6181 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
6241 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
6301 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
6361 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
6421 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
6481 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
6541 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
6601 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
6661 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
6721 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
6781 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
6841 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
6901 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
6961 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
7021 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
7081 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
7141 tcctggcctt ttgctggcct tttgctcaca tgt
//
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pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KAT6A-FLAG质粒载体应用举例
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