| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-KAT6B-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001612 |
| 产品用途: | 3' AAVLINK plasmid for KAT6B expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KAT6B |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-KAT6B-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-KAT6B-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-KAT6B-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-KAT6B-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-KAT6B-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-KAT6B-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-KAT6B-FLAG 7236 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7236)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 20, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7236
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3949
/codon_start=1
/label=KAT6B
/translation="EQSAVYLSGGKTKRGRPDLN*ICTPRQKHPWSLTSR*QWKNRRRL
QKEKPAPVPSGLRRRSRKLGKPCCLKRKTEGKKHVPL*VQTHHQVKNQKMISSNLRKRK
RRRRRKRKKRKKRKGKKKKEEEM*KKIQMVLKAKKKRNQKSPRKKKTLHVWMITKRRRK
RMKSHPTTRTMMPMTRMTATWSLPKWRRKSCPEKASKKYWKTRRLF*TLMCSLVTRTQR
S*WTVAST*QLLVTVSPRSLLGTLKLYPNLTRSHPQENRHRSRTKRTARKSIQSSKRET
QQPWKSTLRLSRPFSL*PRRAANRTTPFRIVPRLKRPVEAYRTTPVQTKVHRLPPRSTI
ANSRTTVAQFHPSTPILASPYVLSTAQVSLLWKTATPKSAQIKVPSQCHLCRTWKPVP*
WMSHQFQIIHSKS*TVDLVTWAVSRAQLRTTKTQAATILLWEAASVETALHRTAAPIAT
SPPAV*HRAAVLSPSRCPTSAGAAACCSKPASAPLRPAASSLLKAVWWRGLRAAASSWL
SAAWLLTSPHPCSWLKSPRRATPTLAYTSEWVRVILGLGITRSRQPPSALPNCSS*LIH
LLIIHCLTAIPLL*LPMQTVPLCPHH*VTQGLFNFLSLHTPSLGDPKHKLP*PHPPT*L
LLQ*ICRRLFCNGTWLHQILASLTAKDCKPRLPARATSP*EPSQRLCHQPLPPISHKSM
GAPRL*PCRVLHGL*RCKEA*T*V*T*CQRQPTMSTL*T*T*TLSTP*MGTACPSQ**T
VATTAIMAI*IKRPNTLCRCRWA*WAPSHMPSSQCRPHPTVT*CTRPPDITAT*TQACP
NSLSMAPT*E"
misc_feature 1653..1658
/label=SDS
misc_feature 1656..1661
/label=SDS
misc_feature 1794..1799
/label=SDS
misc_feature 1797..1802
/label=SDS
misc_feature 1800..1805
/label=SDS
misc_feature 1803..1808
/label=SDS
misc_feature 1849..1854
/label=SDS
misc_feature 1852..1857
/label=SDS
misc_feature 1959..1964
/label=SDS
misc_feature 1962..1967
/label=SDS
misc_feature 3386..3391
/label=SDS
CDS 3956..4021
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 4025..4456
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4169..4174
/label=SDS
repeat_region 4499..4639
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4499..4628
/label=AAV2 ITR
rep_origin 4714..5169
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5451..5555
/gene="bla"
/label=AmpR promoter
CDS 5556..6416
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6587..7175
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggaacaaagc gcggtctatc
1501 taagtggagg caaaacaaag agaggaagac cggatttaaa ctgaatttgt acaccccgcc
1561 agaaacaccc atggagcctg acgagcaggt aacagtggaa gaacagaagg agacttcaga
1621 aggaaaaacc agccccagtc ccatcaggat tgaggaggag gtcaaggaaa ctggggaagc
1681 cctgttgcct caagaggaaa acagaaggga agaaacatgt gcccctgtaa gtccaaacac
1741 atcaccaggt gaaaaaccag aagatgatct catcaaacct gaggaagagg aagaggagga
1801 ggaggaggaa gaggaagaag aggaagaaga ggaaggggaa gaagaagaag gaggaggaaa
1861 tgtagaaaaa gatccagatg gtgctaaaag ccaagaaaaa gaggaaccag aaatctccac
1921 ggaaaaagaa gactctgcac gtttggatga tcacgaagag gaggaggaag aggatgaaga
1981 gccatcccac aacgaggacc atgatgccga tgacgaggat gacagccaca tggagtctgc
2041 cgaagtggag aaggaagagc tgcccagaga aagcttcaaa gaagtactgg aaaaccagga
2101 gactttttta gaccttaatg tgcagcctgg tcactcgaac ccagaggtct taatggactg
2161 tggcgtcgac ctgacagctt cttgtaacag tgagcccaag gagcttgctg gggaccctga
2221 agctgtaccc gaatctgacg aggagccacc cccaggagaa caggcacaga agcaggacca
2281 aaagaacagc aaggaagtcg atacagagtt caaagaggga aacccagcaa ccatggaaat
2341 cgactctgag actgtccagg ccgttcagtc tttgacccag gagagcagcg aacaggacga
2401 cacctttcag gattgtgccg agactcaaga ggcctgtaga agcctacaga actacacccg
2461 tgcagaccaa agtccacaga ttgccaccac gctcgacgat tgccaacagt cggaccacag
2521 tagcccagtt tcatccgtcc actcccatcc tggccagtcc gtacgttctg tcaacagccc
2581 aagtgtccct gctctggaaa acagctacgc ccaaatcagc ccagatcaaa gtgccatctc
2641 agtgccatct ctgcagaaca tggaaaccag tcccatgatg gatgtcccat cagtttcaga
2701 tcattcacag caagtcgtag acagtggatt tagtgacctg ggcagtatcg agagcacaac
2761 tgagaactac gaaaacccaa gcagctacga ttctactatg ggaggcagca tctgtggaaa
2821 cggctcttca cagaacagct gctcctatag caacctcacc tccagcagtc tgacacagag
2881 cagctgtgct gtcacccagc agatgtccaa catcagcggg agctgcagca tgctgcagca
2941 aaccagcatc agctcccctc cgacctgcag cgtcaagtct cctcaaggct gtgtggtgga
3001 gaggcctccg agcagcagcc agcagctggc tcagtgcagc atggctgcta acttcacccc
3061 acccatgcag ctggctgaaa tccccgagac gagcaacgcc aacattggct tatacgagcg
3121 aatgggtcag agtgattttg gggctgggca ttacccgcag ccgtcagcca ccttcagcct
3181 tgccaaactg cagcagttaa ctaatacact tattgatcat tcattgcctt acagccattc
3241 cgctgctgtg acttcctatg caaacagtgc ctctttgtcc acaccattaa gtaacacagg
3301 gcttgttcaa ctttctcagt ctccacactc cgtccctggg ggaccccaag cacaagctac
3361 catgacccca ccccccaacc tgactcctcc tccaatgaat ctgccgccgc ctcttttgca
3421 acggaacatg gctgcatcaa atattggcat ctctcacagc caaagactgc aaacccagat
3481 tgccagcaag ggccacatct ccatgagaac caagtcagcg tctctgtcac cagccgctgc
3541 cacccatcag tcacaaatct atgggcgctc ccagactgta gccatgcagg gtcctgcacg
3601 gactttaacg atgcaaagag gcatgaacat gagtgtgaac ctgatgccag cgccagccta
3661 caatgtcaac tctgtgaaca tgaacatgaa cactctcaac gccatgaatg ggtacagcat
3721 gtcccagcca atgatgaaca gtggctacca cagcaatcat ggctatatga atcaaacgcc
3781 ccaataccct atgcagatgc agatgggcat gatgggcacc cagccatatg cccagcagcc
3841 aatgcagacc ccaccccacg gtaacatgat gtacacggcc cccggacatc acggctacat
3901 gaacacaggc atgtccaaac agtctctcaa tggctcctac atgagaaggt gtacagacta
3961 caaagaccat gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa
4021 gtaactcgag ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
4081 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
4141 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt agttcttgcc
4201 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
4261 actgacaatt ccgtggtgtt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
4321 atctagcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
4381 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat
4441 gtgggaggtt ttttaaagat ctggagaccg gtgtggcacg tgcggaccga gcggccgcag
4501 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
4561 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
4621 gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
4681 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
4741 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg
4801 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
4861 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
4921 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
4981 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
5041 tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt
5101 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
5161 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
5221 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
5281 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
5341 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
5401 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
5461 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
5521 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
5581 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
5641 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
5701 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
5761 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
5821 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
5881 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
5941 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
6001 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
6061 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
6121 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
6181 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
6241 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
6301 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
6361 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
6421 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
6481 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
6541 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
6601 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
6661 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
6721 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
6781 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
6841 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
6901 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
6961 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
7021 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
7081 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
7141 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
7201 ggttcctggc cttttgctgg ccttttgctc acatgt
//
pAAVLINKv1-pCALM1-Cre-3'-KAT6B-FLAG载体图谱质粒图谱pdf版和pAAVLINKv1-pCALM1-Cre-3'-KAT6B-FLAG载体序列质粒序列等相关资料下载
pAAVLINKv1-pCALM1-Cre-3'-KAT6B-FLAG质粒载体应用举例
- 上一篇：pAAVLINK-EF1a-HA-5'-KAT6B
- 下一篇：没有了
