| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-DICER1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001606 |
| 产品用途: | 3' AAVLINK plasmid for DICER1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | DICER1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-DICER1-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-DICER1-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-DICER1-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-DICER1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-DICER1-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-DICER1-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-DICER1-FLAG 6718 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6718)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 20, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6718
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3431
/codon_start=1
/label=DICER1
/translation="GRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEM
LGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDP
PVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEE
ADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPF
SSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQC
IADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNS
QQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEK
KINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPG
VLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSEL
RRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSAN
VPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARR
ALRSLKANQPQVPNS"
misc_feature 1809..1814
/label=SDS
misc_feature 1918..1923
/label=SDS
misc_feature 1921..1926
/label=SDS
misc_feature 1930..1935
/label=SDS
misc_feature 1933..1938
/label=SDS
misc_feature 2125..2130
/label=SDS
misc_feature 2734..2739
/label=SDS
misc_feature 3043..3048
/label=SDS
CDS 3438..3503
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3507..3938
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3651..3656
/label=SDS
repeat_region 3981..4121
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3981..4110
/label=AAV2 ITR
rep_origin 4196..4651
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4933..5037
/gene="bla"
/label=AmpR promoter
CDS 5038..5898
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6069..6657
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggcaggatg gattctgagc
1501 agagcccttc tattgggtac tcctcaagga ctcttggccc caatcctgga cttattcttc
1561 aggctttgac tctgtcaaac gctagtgatg gatttaacct ggagcggctt gaaatgcttg
1621 gcgactcctt tttaaagcat gccatcacca catatctatt ttgcacttac cctgatgcgc
1681 atgagggccg cctttcatat atgagaagca aaaaggtcag caactgtaat ctgtatcgcc
1741 ttggaaaaaa gaagggacta cccagccgca tggtggtgtc aatatttgat ccccctgtga
1801 attggcttcc tcctggttat gtagtaaatc aagacaaaag caacacagat aaatgggaaa
1861 aagatgaaat gacaaaagac tgcatgctgg cgaatggcaa actggatgag gattacgagg
1921 aggaggatga ggaggaggag agcctgatgt ggagggctcc gaaggaagag gctgactatg
1981 aagatgattt cctggagtat gatcaggaac atatcagatt tatagataat atgttaatgg
2041 ggtcaggagc ttttgtaaag aaaatctctc tttctccttt ttcaaccact gattctgcat
2101 atgaatggaa aatgcccaaa aaatcctcct taggtagtat gccattttca tcagattttg
2161 aggattttga ctacagctct tgggatgcaa tgtgctatct ggatcctagc aaagctgttg
2221 aagaagatga ctttgtggtg gggttctgga atccatcaga agaaaactgt ggtgttgaca
2281 cgggaaagca gtccatttct tacgacttgc acactgagca gtgtattgct gacaaaagca
2341 tagcggactg tgtggaagcc ctgctgggct gctatttaac cagctgtggg gagagggctg
2401 ctcagctttt cctctgttca ctggggctga aggtgctccc ggtaattaaa aggactgatc
2461 gggaaaaggc cctgtgccct actcgggaga atttcaacag ccaacaaaag aacctttcag
2521 tgagctgtgc tgctgcttct gtggccagtt cacgctcttc tgtattgaaa gactcggaat
2581 atggttgttt gaagattcca ccaagatgta tgtttgatca tccagatgca gataaaacac
2641 tgaatcacct tatatcgggg tttgaaaatt ttgaaaagaa aatcaactac agattcaaga
2701 ataaggctta ccttctccag gcttttacac atgcctccta ccactacaat actatcactg
2761 attgttacca gcgcttagaa ttcctgggag atgcgatttt ggactacctc ataaccaagc
2821 acctttatga agacccgcgg cagcactccc cgggggtcct gacagacctg cggtctgccc
2881 tggtcaacaa caccatcttt gcatcgctgg ctgtaaagta cgactaccac aagtacttca
2941 aagctgtctc tcctgagctc ttccatgtca ttgatgactt tgtgcagttt cagcttgaga
3001 agaatgaaat gcaaggaatg gattctgagc ttaggagatc tgaggaggat gaagagaaag
3061 aagaggatat tgaagttcca aaggccatgg gggatatttt tgagtcgctt gctggtgcca
3121 tttacatgga tagtgggatg tcactggaga cagtctggca ggtgtactat cccatgatgc
3181 ggccactaat agaaaagttt tctgcaaatg taccccgttc ccctgtgcga gaattgcttg
3241 aaatggaacc agaaactgcc aaatttagcc cggctgagag aacttacgac gggaaggtca
3301 gagtcactgt ggaagtagta ggaaagggga aatttaaagg tgttggtcga agttacagga
3361 ttgccaaatc tgcagcagca agaagagccc tccgaagcct caaagctaat caacctcagg
3421 ttcccaatag ctgtacagac tacaaagacc atgacggtga ttataaagat catgacatcg
3481 actacaagga tgacgatgac aagtaactcg agataatcaa cctctggatt acaaaatttg
3541 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
3601 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
3661 taaatcctgg ttagttcttg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
3721 gacaggggct cggctgttgg gcactgacaa ttccgtggtg tttatttgtg aaatttgtga
3781 tgctattgct ttatttgtaa ccatctagct ttatttgtga aatttgtgat gctattgctt
3841 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
3901 tgtttcaggt tcagggggag atgtgggagg ttttttaaag atctggagac cggtgtggca
3961 cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
4021 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
4081 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
4141 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
4201 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
4261 cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
4321 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
4381 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
4441 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
4501 ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga tttataaggg
4561 attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
4621 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
4681 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
4741 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
4801 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
4861 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
4921 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
4981 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
5041 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
5101 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
5161 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
5221 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
5281 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
5341 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
5401 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
5461 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
5521 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
5581 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
5641 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
5701 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
5761 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
5821 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
5881 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
5941 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
6001 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
6061 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
6121 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
6181 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
6241 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
6301 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
6361 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
6421 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
6481 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
6541 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
6601 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
6661 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
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