| 质粒名称: |
pAAVLINK-3'-MTOR-FLAG-bGHpA
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001696 |
| 产品用途: | 3' AAVLINK plamid for MTOR expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | MTOR |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-3'-MTOR-FLAG-bGHpA
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-3'-MTOR-FLAG-bGHpA载体质粒基本信息
pAAVLINK-3'-MTOR-FLAG-bGHpA质粒图谱载体图谱和pAAVLINK-3'-MTOR-FLAG-bGHpA载体序列质粒序列多克隆位点信息
pAAVLINK-3'-MTOR-FLAG-bGHpA质粒载体简介
pAAVLINK-3'-MTOR-FLAG-bGHpA质粒序列载体序列
LOCUS pAAVLINK-3'-MTOR-FLAG-bGHpA 7323 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7323)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 2, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7323
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
protein_bind 145..178
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 179..270
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 271..4263
/codon_start=1
/label=MTOR
/translation="GYTLADEEEDPLIYQHRMLRSGQGDALASGPVETGPMKKLHVSTI
NLQKAWGAARRVSKDDWLEWLRRLSLELLKDSSSPSLRSCWALAQAYNPMARDLFNAAF
VSCWSELNEDQQDELIRSIELALTSQDIAEVTQTLLNLAEFMEHSDKGPLPLRDDNGIV
LLGERAAKCRAYAKALHYKELEFQKGPTPAILESLISINNKLQQPEAAAGVLEYAMKHF
GELEIQATWYEKLHEWEDALVAYDKKMDTNKDDPELMLGRMRCLEALGEWGQLHQQCCE
KWTLVNDETQAKMARMAAAAAWGLGQWDSMEEYTCMIPRDTHDGAFYRAVLALHQDLFS
LAQQCIDKARDLLDAELTAMAGESYSRAYGAMVSCHMLSELEEVIQYKLVPERREIIRQ
IWWERLQGCQRIVEDWQKILMVRSLVVSPHEDMRTWLKYASLCGKSGRLALAHKTLVLL
LGVDPSRQLDHPLPTVHPQVTYAYMKNMWKSARKIDAFQHMQHFVQTMQQQAQHAIATE
DQQHKQELHKLTARCFLKLGEWQLNLQGINESTIPKVLQYYSAATEHDRSWYKAWHAWA
VMNFEAVLHYKHQNQARDEKKKLRHASGANITNATTAATTAATATTTASTEGSNSESEA
ESTENSPTPSPLQKKVTEDLSKTLLMYTVPAVQGFFRSISLSRGNNLQDTLRVLTLWFD
YGHWPDVNEALVEGVKAIQIDTWLQVIPQLIARIDTPRPLVGRLIHQLLTDIGRYHPQA
LIYPLTVASKSTTTARHNAANKILKNMCEHSNTLVQQAMMVSEELIRVAILWHEMWHEG
LEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYM
KSGNVKDLTQAWDLYYHVFRRISKQLPQLTSLELQYVSPKLLMCRDLELAVPGTYDPNQ
PIIRIQSIAPSLQVITSKQRPRKLTLMGSNGHEFVFLLKGHEDLRQDERVMQLFGLVNT
LLANDPTSLRKNLSIQRYAVIPLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHR
IMLRMAPDYDHLTLMQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLA
VMSMVGYILGLGDRHPSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLT
NAMEVTGLDGNYRITCHTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSR
TRTDSYSAGQSVEILDGVELGEPAHKKTGTTVPESIHSFIGDGLVKPEALNKKAIQIIN
RVRDKLTGRDFSHDDTLDVPTQVELLIKQATSHENLCQCYIGWCPFW"
misc_feature 293..298
/label=SDS
misc_feature 433..438
/label=SDS
misc_feature 1415..1420
/label=SDS
misc_feature 2244..2249
/label=SDS
CDS 2677..2955
/codon_start=1
/product="FKBP-rapamycin binding domain of human FRAP"
/label=FRB
/translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
CDS 2689..2961
/codon_start=1
/product="FKBP-rapamycin binding domain of human FRAP"
/label=FRB
/translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQL"
CDS 4270..4335
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
polyA_signal 4339..4563
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 4586..4726
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4586..4715
/label=AAV2 ITR
rep_origin 4801..5256
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5538..5642
/gene="bla"
/label=AmpR promoter
CDS 5643..6503
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6674..7262
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtataact tcgtatagga tactttatag caatttatga
181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
241 ctgacatcca ctttgccttt ctctccacag ggatacacac ttgctgatga agaggaggat
301 cctttgattt accagcatcg gatgcttagg agtggccaag gggatgcatt ggctagtgga
361 ccagtggaaa caggacccat gaagaaactg cacgtcagca ccatcaacct ccaaaaggcc
421 tggggcgctg ccaggagggt ctccaaagat gactggctgg aatggctgag acggctgagc
481 ctggagctgc tgaaggactc atcatcgccc tccctgcgct cctgctgggc cctggcacag
541 gcctacaacc cgatggccag ggatctcttc aatgctgcat ttgtgtcctg ctggtctgaa
601 ctgaatgaag atcaacagga tgagctcatc agaagcatcg agttggccct cacctcacaa
661 gacatcgctg aagtcacaca gaccctctta aacttggctg aattcatgga acacagtgac
721 aagggccccc tgccactgag agatgacaat ggcattgttc tgctgggtga gagagctgcc
781 aagtgccgag catatgccaa agcactacac tacaaagaac tggagttcca gaaaggcccc
841 acccctgcca ttctagaatc tctcatcagc attaataata agctacagca gccggaggca
901 gcggccggag tgttagaata tgccatgaaa cactttggag agctggagat ccaggctacc
961 tggtatgaga aactgcacga gtgggaggat gcccttgtgg cctatgacaa gaaaatggac
1021 accaacaagg acgacccaga gctgatgctg ggccgcatgc gctgcctcga ggccttgggg
1081 gaatggggtc aactccacca gcagtgctgt gaaaagtgga ccctggttaa tgatgagacc
1141 caagccaaga tggcccggat ggctgctgca gctgcatggg gtttaggtca gtgggacagc
1201 atggaagaat acacctgtat gatccctcgg gacacccatg atggggcatt ttatagagct
1261 gtgctggcac tgcatcagga cctcttctcc ttggcacaac agtgcattga caaggccagg
1321 gacctgctgg atgctgaatt aactgcgatg gcaggagaga gttacagtcg ggcatatggg
1381 gccatggttt cttgccacat gctgtccgag ctggaggagg ttatccagta caaacttgtc
1441 cccgagcgac gagagatcat ccgccagatc tggtgggaga gactgcaggg ctgccagcgt
1501 atcgtagagg actggcagaa aatccttatg gtgcggtccc ttgtggtcag ccctcatgaa
1561 gacatgagaa cctggctcaa gtatgcaagc ctgtgcggca agagtggcag gctggctctt
1621 gctcataaaa ctttagtgtt gctcctggga gttgatccgt ctcggcaact tgaccatcct
1681 ctgccaacag ttcaccctca ggtgacctat gcctacatga aaaacatgtg gaagagtgcc
1741 cgcaagatcg atgccttcca gcacatgcag cattttgtcc agaccatgca gcaacaggcc
1801 cagcatgcca tcgctactga ggaccagcag cataagcagg aactgcacaa gctcacggcc
1861 cgatgcttcc tgaaacttgg agagtggcag ctgaatctac agggcatcaa tgagagcaca
1921 atccccaaag tgctgcagta ctacagcgcc gccacagagc acgaccgcag ctggtacaag
1981 gcctggcatg cgtgggcagt gatgaacttc gaagctgtgc tacactacaa acatcagaac
2041 caagcccgcg atgagaagaa gaaactgcgt catgccagcg gggccaacat caccaacgcc
2101 accactgccg ccaccacggc cgccactgcc accaccactg ccagcaccga gggcagcaac
2161 agtgagagcg aggccgagag caccgagaac agccccaccc catcgccgct gcagaagaag
2221 gtcactgagg atctgtccaa aaccctcctg atgtacacgg tgcctgccgt ccagggcttc
2281 ttccgttcca tctccttgtc acgaggcaac aacctccagg atacactcag agttctcacc
2341 ttatggtttg attatggtca ctggccagat gtcaatgagg ccttagtgga gggggtgaaa
2401 gccatccaga ttgatacctg gctacaggtt atacctcagc tcattgcaag aattgatacg
2461 cccagaccct tggtgggacg tctcattcac cagcttctca cagacattgg tcggtaccac
2521 ccccaggccc tcatctaccc actgacagtg gcttctaagt ctaccacgac agcccggcac
2581 aatgcagcca acaagattct gaagaacatg tgtgagcaca gcaacaccct ggtccagcag
2641 gccatgatgg tgagcgagga gctgatccga gtggccatcc tctggcatga gatgtggcat
2701 gaaggcctgg aagaggcatc tcgtttgtac tttggggaaa ggaacgtgaa aggcatgttt
2761 gaggtgctgg agcccttgca tgctatgatg gaacggggcc cccagactct gaaggaaaca
2821 tcctttaatc aggcctatgg tcgagattta atggaggccc aagagtggtg caggaagtac
2881 atgaaatcag ggaatgtcaa ggacctcacc caagcctggg acctctatta tcatgtgttc
2941 cgacgaatct caaagcagct gcctcagctc acatccttag agctgcaata tgtttcccca
3001 aaacttctga tgtgccggga ccttgaattg gctgtgccag gaacatatga ccccaaccag
3061 ccaatcattc gcattcagtc catagcaccg tctttgcaag tcatcacatc caagcagagg
3121 ccccggaaat tgacacttat gggcagcaac ggacatgagt ttgttttcct tctaaaaggc
3181 catgaagatc tgcgccagga tgagcgtgtg atgcagctct tcggcctggt taacaccctt
3241 ctggccaatg acccaacatc tcttcggaaa aacctcagca tccagagata cgctgtcatc
3301 cctttatcga ccaactcggg cctcattggc tgggttcccc actgtgacac actgcacgcc
3361 ctcatccggg actacaggga gaagaagaag atccttctca acatcgagca tcgcatcatg
3421 ttgcggatgg ctccggacta tgaccacttg actctgatgc agaaggtgga ggtgtttgag
3481 catgccgtca ataatacagc tggggacgac ctggccaagc tgctgtggct gaaaagcccc
3541 agctccgagg tgtggtttga ccgaagaacc aattataccc gttctttagc ggtcatgtca
3601 atggttgggt atattttagg cctgggagat agacacccat ccaacctgat gctggaccgt
3661 ctgagtggga agatcctgca cattgacttt ggggactgct ttgaggttgc tatgacccga
3721 gagaagtttc cagagaagat tccatttaga ctaacaagaa tgttgaccaa tgctatggag
3781 gttacaggcc tggatggcaa ctacagaatc acatgccaca cagtgatgga ggtgctgcga
3841 gagcacaagg acagtgtcat ggccgtgctg gaagcctttg tctatgaccc cttgctgaac
3901 tggaggctga tggacacaaa taccaaaggc aacaagcgat cccgaacgag gacggattcc
3961 tactctgctg gccagtcagt cgaaattttg gacggtgtgg aacttggaga gccagcccat
4021 aagaaaacgg ggaccacagt gccagaatct attcattctt tcattggaga cggtttggtg
4081 aaaccagagg ccctaaataa gaaagctatc cagattatta acagggttcg agataagctc
4141 actggtcggg acttctctca tgatgacact ttggatgttc caacgcaagt tgagctgctc
4201 atcaaacaag cgacatccca tgaaaacctc tgccagtgct atattggctg gtgccctttc
4261 tggtgtacag actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag
4321 gatgacgatg acaagtaact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
4381 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
4441 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
4501 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
4561 tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct
4621 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt
4681 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg
4741 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
4801 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
4861 ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
4921 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
4981 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
5041 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
5101 gactcttgtt ccaaactgga acaacactca actctatctc gggctattct tttgatttat
5161 aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta
5221 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
5281 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
5341 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
5401 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
5461 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
5521 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
5581 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
5641 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
5701 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
5761 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
5821 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
5881 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
5941 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
6001 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
6061 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
6121 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
6181 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
6241 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
6301 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
6361 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
6421 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
6481 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
6541 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
6601 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
6661 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
6721 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
6781 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
6841 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
6901 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
6961 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
7021 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
7081 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
7141 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
7201 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
7261 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
7321 tgt
//
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