| 质粒名称: | pAAVLINK-EF1a-HA-5'-RIMS2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001623 |
| 产品用途: | 5' AAVLINK plasmid for RIMS2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: |
EF1a
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | RIMS2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-RIMS2
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-RIMS2载体质粒基本信息
pAAVLINK-EF1a-HA-5'-RIMS2质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-RIMS2载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-RIMS2质粒载体简介
pAAVLINK-EF1a-HA-5'-RIMS2质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-RIMS2 6776 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6776)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 21, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6776
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3916
/codon_start=1
/label=RIMS2
/translation="MSAPVGPRGRLAPIPAASQPPLQPEMPDLSHLTEEERKIILAVMD
RQKKEEEKEQSVLKVKEEHKPQLTQWFPFSGITELVNNVLQPQQKQQNEKEPQTKLHQQ
FEMYKEQVKKMGEESQQQQEQKGDAPTCGICHKTKFADGCGHNCSYCQTKFCARCGGRV
SLRSNKEDKVVMWVCNLCRKQQEILTKSGAWFYNSGSNTPQQPDQKVLRGLRNEEAPQE
KKPKLHEQTQFQGPSGDLSVPAVEKSRSHGLTRQHSIKNGSGVKHHIASDIASDRKRSP
SVSRDQNRRYDQREEREEYSQYATSDTAMPRSPSDYADRRSQHEPQFYEDSDHLSYRDS
NRRSHRHSKEYIVDDEDVESRDEYERQRREEEYQSRYRSDPNLARYPVKPQPYEEQMRI
HAEVSRARHERRHSDVSLANADLEDSRISMLRMDRPSRQRSISERRAAMENQRSYSMER
TREAQGPSSYAQRTTNHSPPTPRRSPLPIDRPDLRRTDSLRKQHHLDPSSAVRKTKREK
METMLRNDSLSSDQSESVRPPPPKPHKSKKGGKMRQISLSSSEEELASTPEYTSCDDVE
IESESVSEKGDSQKGKRKTSEQAVLSDSNTRSERQKEMMYFGGHSLEEDLEWSEPQIKD
SGVDTCSSTTLNEEHSHSDKHPVTWQPSKDGDRLIGRILLNKRLKDGSVPRDSGAMLGL
KVVGGKMTESGRLCAFITKVKKGSLADTVGHLRPGDEVLEWNGRLLQGATFEEVYNIIL
ESKPEPQVELVVSRPIGDIPRIPDSTHAQLESSSSSFESQKMDRPSISVTSPMSPGMLR
DVPQFLSGQLSIKLWFDKVGHQLIVTILGAKDLPSREDGRPRNPYVKIYFLPDRSDKNK
RRTKTVKKTLEPKWNQTFIYSPVHRREFRERMLEITLWDQARVREEESEFLGEILIELE
TALLDDEPHWYKLQTHDVSSLPLPHPSPYMPRRQLHGESPTRRLQRSKRISDSEVSDYD
CDDGIGVVSDYRHDGRDLQSSTLSVPEQVMSSNHCSPSGSPHRVDVIGRTRSWSPSVPP
PQSRNVEQ"
misc_feature 849..854
/label=SDS
misc_feature 1254..1259
/label=SDS
misc_feature 1398..1403
/label=SDS
misc_feature 2177..2182
/label=SDS
misc_feature 2189..2194
/label=SDS
misc_feature 2427..2432
/label=SDS
misc_feature 2839..2844
/label=SDS
CDS complement(3136..3147)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
misc_feature 3887..3892
/label=SDS
intron 3917..3957
/label=Splicing Donor
misc_feature 3961..3994
/label=lox2272-JT1571
repeat_region 4039..4179
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4039..4168
/label=AAV2 ITR
rep_origin 4254..4709
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4991..5095
/gene="bla"
/label=AmpR promoter
CDS 5096..5956
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6127..6715
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat gtcggctcct gtcgggcccc ggggccgcct
781 ggctcccatc ccggcggcct ctcagccgcc tctgcagccc gagatgcctg acctcagcca
841 cctcacggag gaggagagga aaatcatcct ggccgtcatg gataggcaga agaaagaaga
901 ggagaaggag cagtccgtgc tcaaggtcaa agaagaacac aaaccacaac tgacacagtg
961 gtttcccttt agtggaatca ctgaactggt aaataacgtt cttcagcccc agcaaaaaca
1021 acaaaatgaa aaggagcccc agacaaaact gcatcagcag tttgaaatgt ataaagagca
1081 ggtaaagaag atgggagaag aatcacagca acagcaagaa cagaagggtg atgcgccaac
1141 ctgtggtatc tgccacaaaa caaagtttgc tgatggatgt ggccataact gttcatattg
1201 ccaaacaaag ttctgtgctc gttgtggagg tcgagtgtca ttacgctcaa acaaggagga
1261 caaagtggtt atgtgggtat gtaatttgtg ccgaaaacaa caagaaatcc tcactaaatc
1321 aggagcatgg ttttataata gtggatctaa tacaccacag caacctgatc aaaaggttct
1381 tcgagggcta agaaatgagg aggcacctca ggagaagaaa ccaaaactac atgagcagac
1441 ccagttccaa ggaccctcag gtgacttatc tgtacctgca gtggagaaaa gtcgatctca
1501 tgggctcaca agacagcatt ctattaaaaa tgggtcaggc gtgaagcatc acattgccag
1561 tgacatagct tcagacagga aaagaagccc atctgtgtcc agagatcaga atagaagata
1621 cgaccaaagg gaagaaagag aggaatattc acagtatgct acttcggata ccgcaatgcc
1681 tagatctcca tcagattatg ctgataggcg atctcaacat gaacctcagt tttatgaaga
1741 ctctgatcat ttaagttata gggactccaa caggagaagt cataggcatt ccaaagaata
1801 tattgtagat gatgaggatg tggaaagcag agatgaatac gaaaggcaaa ggagagagga
1861 agagtaccag tcacgctacc gaagtgatcc gaatttggcc cgttatccag taaagccaca
1921 accctatgaa gaacaaatgc ggatccatgc tgaagtgtcc cgagcacggc atgagagaag
1981 gcatagtgat gtttctttgg caaatgctga tctggaagat tccaggattt ctatgctaag
2041 gatggatcga ccatcaaggc aaagatctat atcagaacgt agagctgcca tggaaaatca
2101 gcgatcttat tcaatggaaa gaactcgaga ggctcaggga ccaagttctt atgcacaaag
2161 gaccacaaac catagtcctc ctacccccag gaggagtcca ctacccatag atagaccaga
2221 cttgaggcgt actgactcac tacggaaaca gcaccactta gatcctagct ctgctgtaag
2281 aaaaacaaaa cgggaaaaaa tggaaacaat gttaaggaat gattctctca gttcagacca
2341 gtcagagtca gtgagacctc caccaccaaa gcctcataaa tcaaagaaag gcggtaaaat
2401 gcgccagatt tcgttgagca gttcagagga ggaattggct tccacgcctg aatatacaag
2461 ttgtgatgat gttgagattg aaagtgagag tgtaagtgaa aaaggggaca gtcaaaaggg
2521 aaaaagaaaa actagtgagc aggcagtttt gtcggactct aacaccaggt ctgagagaca
2581 aaaggaaatg atgtactttg gtggccactc tttggaagag gatttggaat ggtctgaacc
2641 tcagattaag gactctgggg tagatacgtg tagtagcaca acccttaacg aggagcatag
2701 ccatagtgat aagcaccctg taacctggca accatctaaa gatggagatc gtttaattgg
2761 tcgcatttta ttaaataagc gtctaaaaga tggaagtgta cctcgagatt caggagcaat
2821 gcttggcttg aaggttgtag gaggaaagat gactgaatca ggtcggcttt gtgcatttat
2881 tactaaagta aaaaaaggaa gtttagctga tactgtagga catcttagac caggtgatga
2941 agtattagaa tggaatggaa gactactgca aggagccaca tttgaggaag tgtacaacat
3001 cattctagaa tccaaacctg aaccacaagt agaacttgta gtttcaaggc ctattggaga
3061 tataccgcga atacctgata gcacacatgc acaactggag tccagttcta gctcctttga
3121 atctcaaaaa atggatcgtc cttctatttc tgttacctct cccatgagtc ctggaatgtt
3181 gagggatgtc ccacagttct tatcaggaca actttcaata aaactatggt ttgacaaggt
3241 tggtcaccaa ttaatagtta caattttggg agcaaaagat ctcccttcca gggaagatgg
3301 gaggccaagg aatccttatg ttaaaattta ctttcttcca gacagaagtg ataaaaacaa
3361 gagaagaact aaaacagtaa agaaaacatt ggaacccaaa tggaaccaaa cattcattta
3421 ttctccagtc caccgaagag aatttcggga acgaatgcta gagattaccc tttgggatca
3481 agctcgtgtt cgagaggaag aaagtgaatt cttaggcgag attttaattg aattagaaac
3541 agcattatta gatgatgagc cacattggta caaacttcag acgcatgatg tctcttcatt
3601 gccacttccc cacccttctc catatatgcc acgaagacag ctccatggag agagcccaac
3661 acggaggttg caaaggtcaa agagaataag tgatagtgaa gtctctgact atgactgtga
3721 tgatggaatt ggtgtagtat cagattatcg acatgatggt cgagatcttc aaagctcaac
3781 attatcagtg ccagaacaag taatgtcatc aaaccactgt tcaccatcag ggtctcctca
3841 tcgagtagat gttataggaa ggactagatc atggtcaccc agtgtccctc ctccacaaag
3901 tcggaatgtg gaacaggtaa gtatcaaggt tacaagacag gtttaaggag accaataagc
3961 tattattcgt ataggatact ttatacgaag ttattaagtt atggagaccg gtgtggcacg
4021 tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg
4081 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
4141 cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc
4201 tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc
4261 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
4321 tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
4381 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
4441 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
4501 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
4561 gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt tataagggat
4621 tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
4681 ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga
4741 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
4801 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
4861 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
4921 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
4981 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
5041 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
5101 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
5161 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
5221 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
5281 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
5341 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
5401 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
5461 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
5521 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
5581 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
5641 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
5701 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
5761 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
5821 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
5881 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
5941 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
6001 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
6061 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
6121 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
6181 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
6241 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
6301 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
6361 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
6421 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
6481 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
6541 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
6601 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
6661 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
6721 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//
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