pAAVLINK-EF1a-HA-5'-NCKAP5

LOCUS       pAAVLINK-EF1a-HA-5'-NCKAP5                6961 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6961)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..141
                     /label=AAV2 ITR
     promoter        198..409
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             422..690
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    516..521
                     /label=SDS
     CDS             727..753
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             760..4101
                     /codon_start=1
                     /label=NCKAP5
                     /translation="MEGKRQLEKRDFGKRLSLDSSLVEYMDSNKYIEHLLTQLEEQHRS
                     LWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQ
                     MRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMV
                     VDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVV
                     QALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSG
                     DLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSL
                     SSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKES
                     HILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLG
                     SPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETN
                     RKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALN
                     LQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEE
                     KQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRN
                     EISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPR
                     VSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQ
                     KSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQ
                     LPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPP
                     PPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATT
                     EVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKT
                     SPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGV
                     NESPSSQ"
     misc_feature    1034..1039
                     /label=SDS
     misc_feature    1811..1816
                     /label=SDS
     misc_feature    2657..2662
                     /label=SDS
     misc_feature    3400..3405
                     /label=SDS
     intron          4102..4142
                     /label=Splicing Donor
     misc_feature    4146..4179
                     /label=lox2272-JT1571
     repeat_region   4224..4364
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4224..4353
                     /label=AAV2 ITR
     rep_origin      4439..4894
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5176..5280
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5281..6141
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6312..6900
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatcta tggagggaaa gagacagctt
      781 gagaaaaggg actttggaaa aaggctgtct ctagacagca gtcttgtgga atacatggac
      841 tccaataaat acattgagca tctgctgact cagcttgagg agcaacacag gagtctctgg
      901 agggagaagt tggcagtggc ccgactacag cgagaagttg cccaaagaac aagtgagggg
      961 gccatgcatg agaagctgat acatgaactg gaagaggaga gacacttacg tcttcaaagc
     1021 gagaagcggt tgcaggaggt gaccctagag tctgaacgca acagaattca gatgcgtagc
     1081 ttgcagcagc agttctccag gatggaagaa acagtacgaa atctattgca gagtcaagga
     1141 tctccagagc agaaaaaaga agaaactgtt aatataatgg tctatcagga aaagctgtca
     1201 gaggaagaga gaaaacataa ggaagctttg gaagatcttc acatggtggt tgatgaggat
     1261 tcgaggagtg aaagcagcag tacagatgag ggaaaagaaa agaccaaatt gctattagag
     1321 agattgaaag ctctagaggc agagaattca gcgttggctt tggagaatga aaatcaaagg
     1381 gaacaatatg agcgatgtct tgatgaggta gccaaccaag ttgttcaagc tctgcttact
     1441 caaaaggatc taagagagga atgtgtgaag ttgaaaacaa gagtgtttga tttggaacag
     1501 cagaatcgaa cactaagcat cctattccaa cagcgagtca gacccacttc tgatctgctc
     1561 ctccagaaac ttcactcacg tctcttggat ctttcatctg gagatttgct ttcagaggtg
     1621 gaaagaaacc gaagtctgac acagtcacga actgatgctg aggtgcacga acaccaacta
     1681 aatacaaaat cggccttgaa atgccctggc ttgggggctg tcatccctgg tcatctctgt
     1741 cctcgaaaca gctacagtag cagcagtgaa ctgtctcttt caagcacctg cagcgagtac
     1801 tccagtggct cctcctacac gtggcacgat gggaagaacc tcaggaaaag gcaatcatca
     1861 caaaactggg ataaaaggct aagtattgat tcttcgctcc caagtggctt tgctagtcct
     1921 acaaatgaac tacctccaac tcgtatcaag gaaagccaca ttttggaagg gctaagaaag
     1981 ctacagaagc gaaaagtgtt acttgaaccc ccatcagtga taaccaaatg gggttataaa
     2041 gattgcatga actcgaatga aggaatatat tctcctggaa ttaaaagtag cagcctcaag
     2101 gagtatcccc cctgcaaaac agctgacctg gggagcccct gcaaggaacc ccacaagaca
     2161 tttgtttatg atctagattc ccacgttgat gcggacgatg acccttccac cttagcattg
     2221 ctccaagcag ttccaaacca gagctgcagg ccacatggca gtaaattaac ccacagtgtt
     2281 tccgacagtc tgtttggctg ggagacaaac agaaaacact ttctggaagg cacatcctca
     2341 gtttatccca aggaaaggcc tgaaaagctg acaagttgcg ccagcagctg tcccttagag
     2401 atgaagctgt gcccaagtgt gcagacgcct caggtacaga gggagagggg cccacagggc
     2461 caaggccatg gccgcatggc tctcaacctc cagctttcag acactgatga caatgaaacg
     2521 tttgatgagc tgcacataga gagcagtgat gagaaaagtc cttcagacgt gtcattggct
     2581 gccgacaccg ataagtccgt ggagaacctg gatgtccttg tggggtttgg aaaatctcta
     2641 tgtgggtctc ctgaagagga ggaaaaacaa gtgcccatcc cttcagagac taggccaaag
     2701 acttttagtt tcattaagca gcaaagagtt gtaaaaagga cttcttcaga agaatgtgtg
     2761 actgtgatat ttgatgcgga agacggtgag cccattgaat tcagctctca ccagactggg
     2821 gttgtcactg ttaccagaaa tgagatttcc atcaattcaa ctcctgctgg acccaaggca
     2881 gaacatactg agcttttacc tcagggaatt gcttgtttac agccaagagc tgctgcaaga
     2941 gactatactt tctttaaaag gtctgaagag gacactgaga aaaacattcc aaaagataat
     3001 gtagataatg ttcccagggt gtccactgaa tctttcagct ccaggacagt gacacaaaat
     3061 cctcagcagc aaaagctggt caaaccaaca cacaatatat catgccagag taattccagg
     3121 tcttcggcac ccatgggcat ctatcaaaag caaaatctga caaaaatacc tcccaggggc
     3181 aagtcttcac ctcagaaatc aaaactaatg gagcccgaag ccaccacact actcccttca
     3241 tctggcctgg tgactcttga aaaatcacca gccttagctc ctgggaaact ctcacgattc
     3301 atgaagactg agagctcagg gcccctcttt gaattacgat cagatccaca cattccaaaa
     3361 cattccgccc aacttccgca cagctccagg atgcccagca ggagggactg ggtccagtgc
     3421 cccaagagtc agactccagg gtcacggtca aggcctgcca ttgagtctag tgacagtgga
     3481 gagcccccca cgagggatga acactgtggc tctgggccgg aggcaggggt gaaatcccct
     3541 tcccctccgc cccctccagg caggtccgtc tccctgctgg ccaggcccag ctatgactat
     3601 tcaccagcac cttcatccac caagtccgaa accagggtcc ccagtgaaac agcaaggacc
     3661 ccattcaaat ccccgctgct gaaaggaatt tctgctccag ttatttcttc taatccggcc
     3721 acgacagaag tgcagaggaa gaaaccttct gtggccttca aaaagcctat cttcactcac
     3781 cctatgccct ccccagaagc agtcattcaa acccgatgcc ctgctcatgc cccctccagc
     3841 tccttcaccg taatggctct ggggcctcca aaggtctctc cgaagagagg tgtccccaaa
     3901 acctctcctc gccaaacact tgggacccca caaagggaca taggattaca gactcccagg
     3961 atctctcctt caacccatga gccactggaa atgacgtcct ccaaaagtgt atctccaggg
     4021 agaaaaggac aattgaatga tagcgcctcc acacccccca agccttcctt cttaggggta
     4081 aatgagtcac catcatctca ggtaagtatc aaggttacaa gacaggttta aggagaccaa
     4141 taagctatta ttcgtatagg atactttata cgaagttatt aagttatgga gaccggtgtg
     4201 gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
     4261 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     4321 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
     4381 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     4441 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4501 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4561 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4621 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     4681 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4741 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa
     4801 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4861 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     4921 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     4981 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     5041 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     5101 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     5161 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5221 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5281 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5341 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5401 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5461 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5521 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5581 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5641 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5701 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5761 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5821 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5881 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5941 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     6001 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     6061 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     6121 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     6181 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6241 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6301 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6361 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6421 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6481 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6541 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6601 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6661 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6721 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6781 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6841 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6901 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6961 t
//