pAAVLINK-EF1a-HA-5'-MYLK

LOCUS       pAAVLINK-EF1a-HA-5'-MYLK              7352 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    505..510
                     /label=SDS
     CDS             716..742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             749..4492
                     /codon_start=1
                     /label=MYLK
                     /translation="MGDVKLVASSHISKTSLSVDPSRVDSMPLTEAPAFILPPRNLCIK
                     EGATAKFEGRVRGYPEPQVTWHRNGQPITSGGRFLLDCGIRGTFSLVIHAVHEEDRGKY
                     TCEATNGSGARQVTVELTVEGSFAKQLGQPVVSKTLGDRFSASAVETRPSIWGECPPKF
                     ATKLGRVVVKEGQMGRFSCKITGRPQPQVTWLKGNVPLQPSARVSVSEKNGMQVLEIHG
                     VNQDDVGVYTCLVVNGSGKASMSAELSIQGLDSANRSFVRETKATNSDVRKEVTNVISK
                     ESKLDSLEAAAKSKNCSSPQRGGSPPWAANSQPQPPRESKLESCKDSPRTAPQTPVLQK
                     TSSSITLQAARVQPEPRAPGLGVLSPSGEERKRPAPPRPATFPTRQPGLGSQDVVSKAA
                     NRRIPMEGQRDSAFPKFESKPQSQEVKENQTVKFRCEVSGIPKPEVAWFLEGTPVRRQE
                     GSIEVYEDAGSHYLCLLKARTRDSGTYSCTASNAQGQVSCSWTLQVERLAVMEVAPSFS
                     SVLKDCAVIEGQDFVLQCSVRGTPVPRITWLLNGQPIQYARSTCEAGVAELHIQDALPE
                     DHGTYTCLAENALGQVSCSAWVTVHEKKSSRKSEYLLPVAPSKPTAPIFLQGLSDLKVM
                     DGSQVTMTVQVSGNPPPEVIWLHNGNEIQESEDFHFEQRGTQHSLCIQEVFPEDTGTYT
                     CEAWNSAGEVRTQAVLTVQEPHDGTQPWFISKPRSVTASLGQSVLISCAIAGDPFPTVH
                     WLRDGKALCKDTGHFEVLQNEDVFTLVLKKVQPWHAGQYEILLKNRVGECSCQVSLMLQ
                     NSSARALPRGREPASCEDLCGGGVGADGGGSDRYGSLRPGWPARGQGWLEEEDGEDVRG
                     VLKRRVETRQHTEEAIRQQEVEQLDFRDLLGKKVSTKTLSEDDLKEIPAEQMDFRANLQ
                     RQVKPKTVSEEERKVHSPQQVDFRSVLAKKGTSKTPVPEKVPPPKPATPDFRSVLGGKK
                     KLPAENGSSSAETLNAKAVESSKPLSNAQPSGPLKPVGNAKPAETLKPMGNAKPAETLK
                     PMGNAKPDENLKSASKEELKKDVKNDVNCKRGHAGTTDNEKRSESQGTAPAFKQKLQDV
                     HVAEGKKLLLQCQVSSDPPATIIWTLNGKTLKTTKFIILSQEGSLCSVSIEKALPEDRG
                     LYKCVAKNDAGQAECSCQVTVDDAPASENTKAPEMKSRRPKSSLPPVLGTESDATVKKK
                     PAPKTPPKAAMPPQIIQFPEDQK"
     misc_feature    1041..1046
                     /label=SDS
     misc_feature    2016..2021
                     /label=SDS
     misc_feature    2289..2294
                     /label=SDS
     misc_feature    3333..3338
                     /label=SDS
     misc_feature    3399..3404
                     /label=SDS
     misc_feature    3417..3422
                     /label=SDS
     misc_feature    3445..3450
                     /label=SDS
     intron          4493..4533
                     /label=Splicing Donor
     misc_feature    4537..4570
                     /label=lox2272-JT1571
     repeat_region   4615..4755
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4615..4744
                     /label=AAV2 ITR
     rep_origin      4830..5285
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5567..5671
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5672..6532
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6703..7291
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
      721 ttatgacgta cctgactatg ctggatctat gggggatgtg aagctggttg cctcgtcaca
      781 catttccaaa acctccctca gtgtggatcc ctcaagagtt gactccatgc ccctgacaga
      841 ggcccctgct ttcattttgc cccctcggaa cctctgcatc aaagaaggag ccaccgccaa
      901 gttcgaaggg cgggtccggg gttacccaga gccccaggtg acatggcaca gaaacgggca
      961 acccatcacc agcgggggcc gcttcctgct ggattgcggc atccggggga ctttcagcct
     1021 tgtgattcat gctgtccatg aggaggacag gggaaagtat acctgtgaag ccaccaatgg
     1081 cagtggtgct cgccaggtga cagtggagtt gacagtagaa ggaagttttg cgaagcagct
     1141 tggtcagcct gttgtttcca aaaccttagg ggatagattt tcagcttcag cagtggagac
     1201 ccgtcctagc atctgggggg agtgcccacc aaagtttgct accaagctgg gccgagttgt
     1261 ggtcaaagaa ggacagatgg gacgattctc ctgcaagatc actggccggc cccaaccgca
     1321 ggtcacctgg ctcaagggaa atgttccact gcagccgagt gcccgtgtgt ctgtgtctga
     1381 gaagaacggc atgcaggttc tggaaatcca tggagtcaac caagatgacg tgggagtgta
     1441 cacgtgcctg gtggtgaacg ggtcggggaa ggcctcgatg tcagctgaac tttccatcca
     1501 aggtttggac agtgccaata ggtcatttgt gagagaaaca aaagccacca attcagatgt
     1561 caggaaagag gtgaccaatg taatctcaaa ggagtcgaag ctggacagtc tggaggctgc
     1621 agccaaaagc aagaactgct ccagccccca gagaggtggc tccccaccct gggctgcaaa
     1681 cagccagcct cagcccccaa gggagtccaa gctggagtca tgcaaggact cgcccagaac
     1741 ggccccgcag actccggtcc ttcagaagac ttccagctcc atcaccctgc aggccgcaag
     1801 agttcagccg gaaccaagag caccaggcct gggggtccta tcaccttctg gagaagagag
     1861 gaagaggcca gctcctcccc gtccagccac cttccccacc aggcagcctg gcctggggag
     1921 ccaagatgtt gtgagcaagg ctgctaacag gagaatcccc atggagggcc agagggattc
     1981 agcattcccc aaatttgaga gcaagcccca aagccaggag gtcaaggaaa atcaaactgt
     2041 caagttcaga tgtgaagttt ccgggattcc aaagcctgaa gtggcctggt tcctggaagg
     2101 cacccccgtg aggagacagg aaggcagcat tgaggtttat gaagatgctg gctcccatta
     2161 cctctgcctg ctgaaagccc ggaccaggga cagtgggaca tacagctgca ctgcttccaa
     2221 cgcccaaggc caggtgtcct gtagctggac cctccaagtg gaaaggcttg ccgtgatgga
     2281 ggtggccccc tccttctcca gtgtcctgaa ggactgcgct gttattgagg gccaggattt
     2341 tgtgctgcag tgctccgtac gggggacccc agtgccccgg atcacttggc tgctgaatgg
     2401 gcagcccatc cagtacgctc gctccacctg cgaggccggc gtggctgagc tccacatcca
     2461 ggatgccctg ccggaggacc atggcaccta cacctgccta gctgagaatg ccttggggca
     2521 ggtgtcctgc agcgcctggg tcaccgtcca tgaaaagaag agtagcagga agagtgagta
     2581 ccttctgcct gtggctccca gcaagcccac tgcacccatc ttcctgcagg gcctctctga
     2641 tctcaaagtc atggatggaa gccaggtcac tatgactgtc caagtgtcag ggaatccacc
     2701 ccctgaagtc atctggctgc acaatgggaa tgagatccaa gagtcagagg acttccactt
     2761 tgaacagaga ggaactcagc acagcctttg tatccaggaa gtgttcccgg aggacacggg
     2821 cacgtacacc tgcgaggcct ggaacagcgc tggagaggtc cgcacccagg ccgtgctcac
     2881 ggtacaagag cctcacgatg gcacccagcc ctggttcatc agtaagcctc gctcagtgac
     2941 agcctccctg ggccagagtg tcctcatctc ctgcgccata gctggtgacc cctttcctac
     3001 cgtgcactgg ctcagagatg gcaaagccct ctgcaaagac actggccact tcgaggtgct
     3061 tcagaatgag gacgtgttca ccctggttct aaagaaggtg cagccctggc atgccggcca
     3121 gtatgagatc ctgctcaaga accgggttgg cgaatgcagt tgccaggtgt cactgatgct
     3181 acagaacagc tctgccagag cccttccacg ggggagggag cctgccagct gcgaggacct
     3241 ctgtggtgga ggagttggtg ctgatggtgg tggtagtgac cgctatgggt ccctgaggcc
     3301 tggctggcca gcaagagggc agggttggct agaggaggaa gacggcgagg acgtgcgagg
     3361 ggtgctgaag aggcgcgtgg agacgaggca gcacactgag gaggcgatcc gccagcagga
     3421 ggtggagcag ctggacttcc gagacctcct ggggaagaag gtgagtacaa agaccctatc
     3481 ggaagacgac ctgaaggaga tcccagccga gcagatggat ttccgtgcca acctgcagcg
     3541 gcaagtgaag ccaaagactg tgtctgagga agagaggaag gtgcacagcc cccagcaggt
     3601 cgattttcgc tctgtcctgg ccaagaaggg gacttccaag acccccgtgc ctgagaaggt
     3661 gccaccgcca aaacctgcca ccccggattt tcgctcagtg ctgggtggca agaagaaatt
     3721 accagcagag aatggcagca gcagtgccga gaccctgaat gccaaggcag tggagagttc
     3781 caagcccctg agcaatgcac agccttcagg gcccttgaaa cccgtgggca acgccaagcc
     3841 tgctgagacc ctgaagccaa tgggcaacgc caagcctgcc gagaccctga agcccatggg
     3901 caatgccaag cctgatgaga acctgaaatc cgctagcaaa gaagaactca agaaagacgt
     3961 taagaatgat gtgaactgca agagaggcca tgcagggacc acagataatg aaaagagatc
     4021 agagagccag gggacagccc cagccttcaa gcagaagctg caagatgttc atgtggcaga
     4081 gggcaagaag ctgctgctcc agtgccaggt gtcttctgac cccccagcca ccatcatctg
     4141 gacgctgaat ggaaagaccc tcaagaccac caagttcatc atcctctccc aggaaggctc
     4201 actctgctcc gtctccatcg agaaggcact gcctgaggac agaggcttat acaagtgtgt
     4261 agccaagaat gacgctggcc aggcggagtg ctcctgccaa gtcactgtgg atgatgctcc
     4321 agccagtgag aacaccaagg ccccagagat gaaatcccgg aggcccaaga gctctcttcc
     4381 tcccgtgcta ggaactgaga gtgatgcgac tgtgaaaaag aaacctgccc ccaagacacc
     4441 tccgaaggca gcaatgcccc ctcagatcat ccagttccct gaggaccaga aggtaagtat
     4501 caaggttaca agacaggttt aaggagacca ataagctatt attcgtatag gatactttat
     4561 acgaagttat taagttatgg agaccggtgt ggcacgtgcg gaccgagcgg ccgcaggaac
     4621 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
     4681 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
     4741 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     4801 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
     4861 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc
     4921 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4981 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     5041 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     5101 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     5161 ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt
     5221 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     5281 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     5341 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     5401 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     5461 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     5521 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     5581 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5641 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     5701 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     5761 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     5821 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     5881 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     5941 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     6001 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     6061 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     6121 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     6181 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     6241 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     6301 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     6361 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     6421 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     6481 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     6541 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6601 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6661 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6721 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6781 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6841 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6901 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6961 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     7021 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     7081 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     7141 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     7201 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     7261 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     7321 cctggccttt tgctggcctt ttgctcacat gt
//