pAAVLINK-EF1a-HA-5'-DOCK1

LOCUS       pAAVLINK-EF1a-HA-5'-DOCK1                 7287 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7287)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        175..386
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             399..667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    493..498
                     /label=SDS
     CDS             704..730
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             737..4449
                     /codon_start=1
                     /label=DOCK1
                     /translation="TRWVPTKREEKYGVAFYNYDARGADELSLQIGDTVHILETYEGWY
                     RGYTLRKKSKKGIFPASYIHLKEAIVEGKGQHETVIPGDLPLIQEVTTTLREWSTIWRQ
                     LYVQDNREMFRSVRHMIYDLIEWRSQILSGTLPQDELKELKKKVTAKIDYGNRILDLDL
                     VVRDEDGNILDPELTSTISLFRAHEIASKQVEERLQEEKSQKQNIDINRQAKFAATPSL
                     ALFVNLKNVVCKIGEDAEVLMSLYDPVESKFISENYLVRWSSSGLPKDIDRLHNLRAVF
                     TDLGSKDLKREKISFVCQIVRVGRMELRDNNTRKLTSGLRRPFGVAVMDVTDIINGKVD
                     DEDKQHFIPFQPLALDDAIRHKPLNMSSRFSPRVAGENDFLQTVINKVIAAKEVNHKGQ
                     GLWVTLKLLPGDIHQIRKEFPHLVDRTTAVARKTGFPEIIMPGDVRNDIYVTLVQGDFD
                     KGSKTTAKNVEVTVSVYDEDGKRLEHVIFPGAGDEAISEYKSVIYYQVKQPRWFETVKV
                     AIPIEDVNRSHLRFTFRHRSSQDSKDKSEKIFALAFVKLMRYDGTTLRDGEHDLIVYKA
                     EAKKLEDAATYLSLPSTKAELEEKGHSATGKSMQSLGSCTISKDSFQISTLVCSTKLTQ
                     NVDLLGLLKWRSNTSLLQQNLRQLMKVDGGEVVKFLQDTLDALFNIMMENSESETFDTL
                     VFDALVFIIGLIADRKFQHFNPVLETYIKKHFSATLAYTKLTKVLKNYVDGAEKPGVNE
                     QLYKAMKALESIFKFIVRSRILFNQLYENKGEADFVESLLQLFRSINDMMSSMSDQTVR
                     VKGAALKYLPTIVNDVKLVFDPKELSKMFTEFILNVPMGLLTIQKLYCLIEIVHSDLFT
                     QHDCREILLPMMTDQLKYHLERQEDLEACCQLLSHILEVLYRKDVGPTQRHVQIIMEKL
                     LRTVNRTVISMGRDSELIGNFVACMTAILRQMEDYHYAHLIKTFGKMRTDVVDFLMETF
                     IMFKNLIGKNVYPFDWVIMNMVQNKVFLRAINQYADMLNKKFLDQANFELQLWNNYFHL
                     AVAFLTQESLQLENFSSAKRAKILNKYGDMRRQIGFEIRDMWYNLGQHKIKFIPEMVGP
                     ILEMTLIPETELRKATIPIFFDMMQCEFHSTRSFQMFENEIITKLDHEVEGGRGDEQYK
                     VLFDKILLEHCRKHKYLAKTGETFVKLVVRLMERLLDYRTIMHDENKENRMSCTVNVLN
                     FYKEIEREEMYI"
     misc_feature    3417..3422
                     /label=SDS
     misc_feature    4207..4212
                     /label=SDS
     intron          4450..4490
                     /label=Splicing Donor
     misc_feature    4494..4527
                     /label=lox2272-JT1571
     repeat_region   4572..4690
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4572..4679
                     /label=AAV2 ITR
     rep_origin      4765..5220
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5502..5606
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5607..6467
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6638..7226
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
      181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
      241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
      421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
      481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
      541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
      601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
      661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
      721 tgactatgct ggatctacgc gctgggtgcc caccaagcgc gaggagaagt acggcgtggc
      781 tttttataac tatgatgcca gaggagcgga tgaactttct ttacagatcg gagacactgt
      841 gcacatctta gaaacatatg aagggtggta ccgaggttac acgttacgaa aaaagtctaa
      901 gaagggtata tttcctgctt catatattca tcttaaagaa gcgatagttg aaggaaaagg
      961 gcaacatgaa acagtcatcc cgggtgacct ccccctcatc caggaagtca ccacgacact
     1021 ccgagagtgg tccaccatct ggaggcagct ctacgtgcaa gataacaggg agatgtttcg
     1081 aagtgtgcgg cacatgatct atgaccttat tgaatggcga tcacaaattc tttctggaac
     1141 tctgcctcag gatgaactca aagaactgaa gaagaaggtc acagccaaaa ttgattatgg
     1201 aaacagaatt ctagatttgg acctggtggt tagagatgaa gatgggaata ttttggatcc
     1261 agaattaact agcacgatta gtctcttcag agctcatgaa atagcttcta aacaagtgga
     1321 ggaaaggtta caagaggaaa aatctcaaaa gcagaacata gatattaaca gacaagccaa
     1381 gtttgctgca accccttctc tggccttgtt tgtgaacctc aaaaatgtgg tttgtaaaat
     1441 aggagaagat gctgaagtcc tcatgtctct atatgaccct gtggagtcca aattcatcag
     1501 tgagaactac ctggttcgct ggtccagttc aggattacct aaagacatag acagattaca
     1561 taatttgcga gccgtgttta ctgacctcgg aagcaaagac ctgaaaaggg agaaaatcag
     1621 ttttgtctgt cagattgttc gcgtgggtcg catggagctg agggacaaca acaccaggaa
     1681 actgacctcg gggttgcggc gaccttttgg agtggctgtg atggatgtaa cagatataat
     1741 aaatggaaaa gtagatgatg aagataagca gcatttcatt ccctttcagc cgctcgcgtt
     1801 ggacgacgcc attcgacaca agccgctgaa catgtcatcc cgtttttcac ccagggtggc
     1861 aggggagaat gacttccttc agactgttat aaacaaagtc atcgctgcca aagaagtcaa
     1921 ccacaagggg cagggtttgt gggtaacatt gaaattactt cctggagata tccatcagat
     1981 ccgaaaagag tttccgcatt tagtggacag gaccacagct gtggctcgaa aaacagggtt
     2041 tccggagata atcatgcctg gtgatgttcg aaatgatatc tatgtaacat tagttcaagg
     2101 agattttgat aaaggaagca aaacaacagc gaagaacgtg gaggtcacgg tgtctgtgta
     2161 cgatgaggat gggaaacgat tagagcatgt gattttcccg ggtgctggtg atgaagcgat
     2221 ttcagagtac aaatctgtga tttactacca agtaaagcag ccacgctggt ttgagactgt
     2281 taaggtggcc attcccatcg aggacgttaa ccgcagtcac cttcggttta ccttccgcca
     2341 caggtcatca caggactcta aggataaatc tgagaaaata tttgcactag catttgtcaa
     2401 gctgatgaga tacgatggta ccaccctgcg agacggagag cacgatctta tcgtctataa
     2461 ggccgaagca aagaagctgg aagatgctgc cacgtacttg agtctgccct ccacgaaggc
     2521 agagttggaa gaaaagggcc actcggccac cggcaagagc atgcagagcc ttgggagctg
     2581 caccattagc aaggactcct tccagatctc cacgctcgtg tgctccacca aactgactca
     2641 gaacgtggac cttctggggc tcttgaaatg gcgctccaac accagcctgc tgcagcagaa
     2701 cttgaggcag ctgatgaaag tcgatggtgg tgaagtagtg aagtttcttc aggacacgtt
     2761 ggatgccctc ttcaacatca tgatggagaa ctcagagagt gagacttttg acacgttagt
     2821 ctttgatgct ctggtattta tcattggact gattgctgat agaaaatttc agcattttaa
     2881 tcctgttttg gaaacttaca ttaagaaaca ctttagtgca acgttagcct acacgaagtt
     2941 gacaaaagtg ttgaagaact acgtggacgg tgctgagaag ccgggagtaa atgagcagct
     3001 gtacaaagcc atgaaagcgc tagaatccat cttcaagttc atcgtgcgct ccaggatcct
     3061 gttcaatcaa ctgtatgaaa acaagggaga ggctgacttc gtggaatctt tgctgcagct
     3121 cttcaggtcc atcaatgaca tgatgagcag catgtcagac cagaccgtcc gggtgaaggg
     3181 ggcagcactg aaatacttac caacgatcgt caacgatgtg aaattggtgt ttgatcccaa
     3241 agagctcagc aaaatgttta ctgaattcat cctcaatgtt cccatgggct tgctgaccat
     3301 ccagaaactc tactgcttga tcgaaatcgt ccacagtgac ctcttcacac agcatgactg
     3361 cagagagatc ctgcttccca tgatgaccga tcagctcaag taccatctgg agagacagga
     3421 ggacctggag gcctgctgtc agctgctcag ccacatcctg gaggtgctgt acaggaagga
     3481 cgtggggcca acccagaggc acgtccagat tatcatggag aaacttctcc ggaccgtgaa
     3541 ccgaaccgtc atttccatgg gacgggattc tgaactcatt ggaaacttcg tggcttgcat
     3601 gacagctatt ttacgacaaa tggaagatta ccattatgcc cacttgatca agacttttgg
     3661 gaaaatgagg actgatgtgg tagatttcct aatggaaaca ttcatcatgt ttaagaacct
     3721 cattggaaag aacgtttacc ccttcgactg ggtgatcatg aacatggtgc aaaataaagt
     3781 cttcctgcga gcaattaatc agtatgcaga tatgctgaac aaaaaatttc tggatcaagc
     3841 caactttgag ctacagctgt ggaacaacta ctttcacctg gctgttgctt tccttactca
     3901 agagtccctg caactggaga atttttcaag tgccaagaga gccaaaatcc ttaacaagta
     3961 cggagatatg aggagacaga ttggctttga aatcagagac atgtggtaca accttggtca
     4021 acacaagata aagttcattc cagaaatggt gggcccaata ttagaaatga cattaattcc
     4081 cgagacggag ctgcgcaaag ccaccatccc catcttcttt gatatgatgc agtgtgaatt
     4141 ccattcgacc cgaagcttcc aaatgtttga aaatgagatc atcaccaagc tggatcatga
     4201 agtcgaagga ggcagaggag acgaacagta caaagtgtta tttgataaaa tccttctgga
     4261 acactgcagg aagcacaaat acctcgccaa aacaggagaa acttttgtaa aactcgttgt
     4321 gcgcttaatg gaaaggcttt tggattatag aaccatcatg cacgacgaga acaaagaaaa
     4381 ccgcatgagc tgcaccgtca atgtgctgaa tttctacaaa gaaattgaaa gagaagaaat
     4441 gtatataagg taagtatcaa ggttacaaga caggtttaag gagaccaata agctattatt
     4501 cgtataggat actttatacg aagttattaa gttatggaga ccggtgtggc acgtgcggac
     4561 cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
     4621 gctcactgag gccgggcgac caaaggtcgc ccggcctcag tgagcgagcg agcgcgcagc
     4681 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     4741 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4801 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg
     4861 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4921 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4981 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5041 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta
     5101 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa
     5161 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
     5221 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     5281 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     5341 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     5401 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     5461 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5521 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5581 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5641 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5701 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5761 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5821 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5881 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5941 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6001 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6061 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6121 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6181 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6241 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6301 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6361 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6421 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6481 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6541 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6601 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6661 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6721 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6781 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6841 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6901 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6961 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7021 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7081 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7141 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7201 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7261 ccttttgctg gccttttgct cacatgt
//