pLZ358

LOCUS       pLZ358              8595 bp DNA     circular SYN 14-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8595)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 19, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             117..750
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    752..769
                     /label=primer binding site
     misc_feature    797..922
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1415..1648
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1833..1877
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2164..2281
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2410..2492
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     misc_feature    2494..3560
                     /label=P_EF1a
     intron          2613..3551
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             3595..4644
                     /codon_start=1
                     /locus_tag="NLS-Cre"
                     /product="NLS-Cre"
                     /label=NLS-Cre
                     /translation="MPKKKRKVSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFS
                     EHTWKMLLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNM
                     LHRRSGLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQ
                     DIRNLAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSL
                     GVTKLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYG
                     AKDDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETG
                     AMVRLLEDGD"
     CDS             3598..3618
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     4620..4640
                     /label=194-WKG-620-F
     misc_feature    4695..5281
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     regulatory      5294..5303
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5300..5698
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAMPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             5710..5916
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="PDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGF
                     TVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    6087..6117
                     /label=3'PPT
                     /note="3' polypurine tract of HIV-1"
     LTR             6119..6352
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6509..6733
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      6776..6785
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        6829..6933
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6934..7794
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7965..8553
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cggaccgcca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
       61 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
      121 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
      181 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
      241 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
      301 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
      361 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
      421 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
      481 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
      541 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
      601 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
      661 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
      721 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
      781 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
      841 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
      901 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
      961 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     1021 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     1081 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     1141 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     1201 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     1261 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     1321 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     1381 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     1441 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     1501 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     1561 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     1621 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     1681 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     1741 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     1801 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     1861 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     1921 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     1981 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     2041 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     2101 gatccattcg attagtgaac ggatctcgac ggtatcgcca caaatggcag tattcatcca
     2161 caattttaaa agaaaggggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     2221 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     2281 tcgggtttat tacagggaca gcagagatcc actttggatc gataagcttt gcaaagatgg
     2341 ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga
     2401 gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     2461 aagttggggg gaggggtcgg caattgaacc ggtgggaaag tgatgtcgtg tactggctcc
     2521 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2581 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2641 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2701 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2761 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2821 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2881 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2941 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     3001 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     3061 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     3121 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3181 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3241 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3301 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3361 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3421 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3481 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3541 ttccatttca ggtgtcgtga gctagcgttt aaacttaagc ttggtaccgc caccatgccc
     3601 aagaagaaga ggaaggtgtc caatctcctg actgttcacc agaacctccc tgcgctgcca
     3661 gtagatgcca ctagcgatga ggtcaggaaa aatctcatgg atatgtttag ggatagacag
     3721 gcgttttctg aacacacctg gaaaatgctg cttagcgtgt gccgatcctg ggcagcctgg
     3781 tgtaagctga acaatcgcaa atggttcccc gccgagccgg aggacgtgcg cgattacctg
     3841 ctgtatctcc aggcaagagg gctggctgtc aagactatcc agcagcactt gggccaactg
     3901 aatatgctgc atcgacgcag cgggctcccc cggcctagcg attcaaacgc agtctccctt
     3961 gttatgagga gaattagaaa ggaaaacgta gatgcgggtg agagggctaa gcaggctctc
     4021 gcttttgagc ggactgattt cgaccaggtc agatccctga tggagaacag cgatcggtgc
     4081 caggacatca ggaacctcgc atttctggga attgcatata acacacttct gcgcatagct
     4141 gagatcgccc ggatcagagt gaaagacatc agtcgaacgg acggcggccg gatgcttatt
     4201 catattggac gcacaaagac attggtcagc accgctggcg ttgaaaaggc cttgtccctg
     4261 ggcgtaacga agctggtgga aagatggatc tcagtgtccg gcgtggctga cgaccctaat
     4321 aattacttgt tctgtcgagt gagaaaaaac ggagtcgccg cgccctctgc caccagccaa
     4381 ttgagtacac gggcccttga agggatcttt gaggcaaccc accgactcat atacggagcc
     4441 aaggatgaca gtggccagag gtatctcgcc tggtcaggtc attctgctag ggtgggggcc
     4501 gcacgagaca tggcgcgggc aggagtctcc ataccagaga ttatgcaagc tggaggttgg
     4561 acaaatgtga acatcgttat gaactatatc cgcaatcttg actctgaaac cggggccatg
     4621 gtgagactgc tcgaagatgg tgactgagga tccgcatgca tctagggcgg ccaattccgc
     4681 ccctctcccc cccccccctc tccctccccc ccccctaacg ttactggccg aagccgcttg
     4741 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     4801 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     4861 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     4921 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     4981 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     5041 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     5101 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     5161 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     5221 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataagc ttgccacaac
     5281 ccacaaggag acggccacca tggccatgcc tttgtctcaa gaagaatcca ccctcattga
     5341 aagagcaacg gctacaatca acagcatccc catctctgag gactacagcg tcgccagcgc
     5401 agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg
     5461 accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac
     5521 ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg
     5581 acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca
     5641 gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaata
     5701 agcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     5761 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     5821 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     5881 cgcacctggt gcatgacccg caagcccggt gcctgataat aggcggccgc tcgagaccta
     5941 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     6001 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     6061 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     6121 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     6181 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6241 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6301 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     6361 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     6421 aggccttgac attataatag atttagcagg aattgaacta ggagtggagc acacaggcaa
     6481 agttctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     6541 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     6601 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     6661 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     6721 gtgggctcta tggcttctga ggcggaaaga accagctggg ggcgcgcccc tcgaggccgc
     6781 catggtcata gctgtttgac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     6841 atttgtttat ttttctaaat acattcaaat atgtatccgc tcacgagaca ataaccctga
     6901 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     6961 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     7021 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7081 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7141 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     7201 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     7261 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     7321 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     7381 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     7441 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     7501 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7561 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7621 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     7681 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     7741 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     7801 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     7861 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     7921 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7981 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8041 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8101 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8161 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8221 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8281 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8341 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8401 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8461 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8521 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8581 ttctggcctt ttgca
//