| 质粒名称: | pLYL145 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000601 |
| 产品用途: | Mammalian cAMP-responsive Cre-repressible 8xANR-NSP3 expression vector (PCRE-loxP-(ANR)8-NSP3-loxP-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | pCRE |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | (ANR)8-NSP3 |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Multi-layered computational gene networks by engineered tristate logics
Shao J., Qiu X., Zhang L., Li S., Xue S., Si Y., Li Y., Jiang J., Wu Y., Xiong Q., Wang Y., Chen Q., Gao T., Zhu L., Wang H., Xie M.
Cell 187, 5064-5080.e14 (2024)
|
pLYL145
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pLYL145载体质粒基本信息
pLYL145质粒图谱载体图谱和pLYL145载体序列质粒序列多克隆位点信息
pLYL145质粒载体简介
pLYL145质粒序列载体序列
LOCUS pLYL145 7887 bp DNA circular SYN 13-JAN-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7887)
AUTHORS iGEM CPU-CHINA 2020
TITLE Direct Submission
JOURNAL Exported Jul 18, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7887
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 235..251
/label=oMH18
misc_feature 252..405
/label=Transcription blocker
/label=Transcription\blocker
polyA_signal 252..300
/note="synthetic polyadenylation signal"
misc_feature 314..405
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
misc_feature 439..525
/label=CRE reg_Regulatory_Seq
misc_feature 450..457
/label=CRE
misc_feature 486..493
/label=CRE*
misc_feature 508..515
/label=CRE
promoter 532..678
/label=P_TAL
primer_bind 635..662
/label=180-WKG-592-F
misc_recomb 694..727
/label=LoxP
protein_bind complement(694..727)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
misc_feature 737..738
/label=NheI
misc_feature 749
/label=New Feature
misc_feature 749
/label=New Feature
regulatory 755..764
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 763
/codon_start=1
/label=5xGS Linker
/translation=""
CDS 764..826
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 827..901
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 902..964
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 965..1039
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1046..1108
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1109..1183
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1184..1246
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1247..1321
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1328..1390
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1391..1465
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1466..1528
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1529..1603
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1610..1672
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1673..1747
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1748..1810
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1811..1885
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
misc_feature 1892..2833
/label=NSP3
misc_feature 1892..1915
/label=NSP3
primer_bind complement(1903..1922)
/label=179-WKG-592-R
polyA_signal 2879..3103
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
polyA_signal 3114..3235
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_recomb 3333..3366
/label=LoxP
protein_bind complement(3333..3366)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
polyA_signal 3373..3421
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
polyA_signal 3484..3708
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3754..4182
/label=f1 bacteriophage origin of replication
/label=f1 bacteriophage origin of replication; arrow _f1
ori
promoter 4196..4525
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
misc_feature 4428..4477
/label=P_SV40 TSS-region
misc_feature 4492..4497
/label=SDS
CDS 4592..5386
/label=Translation 2136-2930
misc_feature 4592..5383
/label=Kan/neoR
/label=nonstandard type: resistance
polyA_signal 5560..5681
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5726..5746)
/label=M13-rev
primer_bind complement(5730..5746)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_binding complement(5752..5774)
/label=LacO
protein_bind 5754..5770
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5778..5808)
/label=lac promoter
protein_bind 5823..5844
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 5950..6890
/label=ColE1
/note="ColE1"
misc_feature complement(6894..7751)
/label=AmpR
/label=nonstandard type: resistance
promoter complement(7752..7856)
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtggtactt
241 ggagcggccg caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg
301 aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa
361 aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctctatcga taggtaccga
421 gctcttacgc gtgctagcgc accagacagt gacgtcagct gccagatccc atggccgtca
481 tactgtgacg tctttcagac accccattga cgtcaatggg agaacagatc tgccgccccg
541 actgcatctg cgtgttcgaa ttcgccaatg acaagacgct gggcggggtt tgtgtcatca
601 tagaactaaa gacatgcaaa tatatttctt ccggggacac cgccagcaaa cgcgagcaac
661 gggccacggg gatgaagcag aaggctagcg cttataactt cgtatagcat acattatacg
721 aagttatcgg cgcgccgcgt ttaaacttaa gcttgccacc atgggggagc tcgacgagtt
781 ggtttatctc ttagacggac ccggatatga ccccattcat tcagatggct ccggaacggg
841 cagcgggacc ggaagtggca ccggaaccac ttccggaacc ggtactggcg ggagtaccgg
901 cggtgagcta gacgaactcg tgtacctgtt ggatggcccc ggttacgacc caatccacag
961 cgatggaagt ggaactgggt ctgggactgg gtccggcacc gggactactt caggcaccgg
1021 cactgggggc tccactggcc ataccgggga gctcgacgag ttggtttatc tcttagacgg
1081 acccggatat gaccccattc attcagatgg ctccggaacg ggcagcggga ccggaagtgg
1141 caccggaacc acttccggaa ccggtactgg cgggagtacc ggcggtgagc tagacgaact
1201 cgtgtacctg ttggatggcc ccggttacga cccaatccac agcgatggaa gtggaactgg
1261 gtctgggact gggtccggca ccgggactac ttcaggcacc ggcactgggg gctccactgg
1321 ccataccggg gagctcgacg agttggttta tctcttagac ggacccggat atgaccccat
1381 tcattcagat ggctccggaa cgggcagcgg gaccggaagt ggcaccggaa ccacttccgg
1441 aaccggtact ggcgggagta ccggcggtga gctagacgaa ctcgtgtacc tgttggatgg
1501 ccccggttac gacccaatcc acagcgatgg aagtggaact gggtctggga ctgggtccgg
1561 caccgggact acttcaggca ccggcactgg gggctccact ggccataccg gggagctcga
1621 cgagttggtt tatctcttag acggacccgg atatgacccc attcattcag atggctccgg
1681 aacgggcagc gggaccggaa gtggcaccgg aaccacttcc ggaaccggta ctggcgggag
1741 taccggcggt gagctagacg aactcgtgta cctgttggat ggccccggtt acgacccaat
1801 ccacagcgat ggaagtggaa ctgggtctgg gactgggtcc ggcaccggga ctacttcagg
1861 caccggcact gggggctcca ctggccatat gatgctcaag atggagtcta cacagcagat
1921 ggctagttcg attattaaca cttcttttga agccgcagtc gttgctgcca cttcaactct
1981 tgaacttatg ggcattcaat atgattataa tgaagtttat actagagtta agagcaaatt
2041 tgattacgta atggatgatt caggtgttaa aaacaatctg ttgggtaaag cggcaactat
2101 tgatcaggca ctgaatggaa aatttggttc agcagcaaga aatagaaatt ggatggctga
2161 tactagaact acagctagac tagatgaaga tgtgaacaaa cttagaatga tgctatcatc
2221 taaaggaatt gatcaaaaaa tgagagtact taatgcatgt ttcaatgtga agcgagttcc
2281 aggtaaatca tcatcaataa ttaaatgtac tagattaatg agagataaga ttgaacgtgg
2341 tgaagtcgaa gtggatgatt cgtttgttga agaaaaaatg gaagttgaca caattgattg
2401 gaaatctaga tatgaacaat tggaaaaacg atttgaatcg ctgaaacaaa gggtgaatga
2461 aaaatataca agttgggtgc aaaaagcaaa aaaggtaaat gaaaatatgt actctctcca
2521 aaacgttata tcacaacagc agagccagat tgctgattta caaaattact gtaataaatt
2581 ggaagttgat ctacaaaata aaattagttc acttgtgtct tcagtcgaat ggtacctgaa
2641 atcaatggaa ttgcctgatg aaattaagac agatattgaa caacaactaa attccattga
2701 tgtaatcaat ccaattaatg ctatagatga ttttgaatca ctgatcagaa atattatact
2761 cgactatgac agaatatttt taatgtttaa aggattaatg aggcaatgca actatgaata
2821 tacgtatgaa taggatccac tagtccagtg tggtgcctgc agggctgatc agcctcgact
2881 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
2941 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
3001 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
3061 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggataagata
3121 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
3181 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
3241 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
3301 cgcttggtac cgtctttcca gtggttaatt aaataacttc gtatagcata cattatacga
3361 agttatggat ccgaacaaac gacccaacac ccgtgcgttt tattctgtct ttttattgcc
3421 gatcctcgag atgctccgaa tgcatcagtg ccgggcccgt ttaaacccgc tgatcagcct
3481 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
3541 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
3601 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
3661 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
3721 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
3781 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
3841 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
3901 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
3961 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
4021 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
4081 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
4141 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
4201 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
4261 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
4321 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
4381 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
4441 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
4501 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
4561 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
4621 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
4681 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
4741 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
4801 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
4861 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
4921 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
4981 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
5041 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
5101 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
5161 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
5221 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
5281 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
5341 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
5401 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
5461 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
5521 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
5581 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
5641 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
5701 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
5761 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
5821 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
5881 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
5941 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
6001 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
6061 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
6121 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
6181 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
6241 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
6301 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
6361 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
6421 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
6481 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
6541 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
6601 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
6661 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
6721 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
6781 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
6841 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
6901 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
6961 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
7021 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
7081 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
7141 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
7201 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
7261 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
7321 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
7381 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
7441 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
7501 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
7561 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
7621 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
7681 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
7741 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
7801 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
7861 tttccccgaa aagtgccacc tgacgtc
//
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