| 质粒名称: | pLYL123 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000600 |
| 产品用途: | Mammalian VanR-specific reporter vector with one target gene (SEAP) placed between parallel loxP sites and another target gene (NanoLuc) placed downstream of a loxP-flanked STOP signal. ((VanO)5-Pmin-loxP-SEAP-pA-loxP-NanoLuc-pA) |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | VanO5-Pmin |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | SEAP |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Multi-layered computational gene networks by engineered tristate logics
Shao J., Qiu X., Zhang L., Li S., Xue S., Si Y., Li Y., Jiang J., Wu Y., Xiong Q., Wang Y., Chen Q., Gao T., Zhu L., Wang H., Xie M.
Cell 187, 5064-5080.e14 (2024)
|
pLYL123
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pLYL123载体质粒基本信息
pLYL123质粒图谱载体图谱和pLYL123载体序列质粒序列多克隆位点信息
pLYL123质粒载体简介
pLYL123质粒序列载体序列
LOCUS pLYL123 7818 bp DNA circular SYN 10-DEC-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7818)
AUTHORS iGEM CPU-CHINA 2020
TITLE Direct Submission
JOURNAL Exported Jul 18, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7818
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 214..238
/label=olyl219
misc_feature 228
/label=E2F-1
misc_feature 232
/label=CMV
misc_feature 234..245
/label=VanO
/label=nonstandard type: operator
misc_feature 248..259
/label=VanO
/label=nonstandard type: operator
misc_feature 262..273
/label=VanO
/label=nonstandard type: operator
misc_feature 276..287
/label=VanO
/label=nonstandard type: operator
misc_feature 290..301
/label=VanO
/label=nonstandard type: operator
primer_bind 302..323
/label=Oscl23-1
misc_feature 302..306
/label=E2F-1
misc_feature 307..455
/label=hcmvmin
misc_feature 377..391
/label=New Feature
primer_bind complement(440..455)
/label=oYW11
primer_bind complement(461..506)
/label=olyl368
primer_bind complement(461..486)
/label=olyl295
primer_bind complement(461..486)
/label=olyl300
misc_feature 478..485
/label=New Feature
primer_bind 484..531
/label=olyl285
misc_feature 488..492
/label=NheI
misc_recomb 497..530
/label=LoxP
protein_bind complement(497..530)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
misc_feature 590..2149
/label=SEAP
CDS 590..2092
/codon_start=1
/product="secreted alkaline phosphatase from human
placenta"
/label=SEAP
/note="The natural enzyme has a C-terminal transmembrane
sequence that is replaced by a GPI anchor."
/translation="MLLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQP
AQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDK
HVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVV
TTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRK
YMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTH
LMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESR
AYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKA
RDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFA
RGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
primer_bind complement(635..656)
/label=222
primer_bind complement(2133..2185)
/label=olyl293
primer_bind 2166..2185
/label=olyl294
polyA_signal 2181..2405
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(2392..2413)
/label=olyl286
primer_bind 2394..2446
/label=olyl287
polyA_signal 2416..2537
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2579..2603)
/label=olyl288
primer_bind 2584..2625
/label=olyl289
misc_recomb 2635..2668
/label=LoxP
protein_bind complement(2635..2668)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
polyA_signal 2675..2723
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
primer_bind complement(2695..2726)
/label=olyl290
primer_bind 2707..2763
/label=olyl291
primer_bind 2733..2762
/label=olyl054
misc_feature 2739..2807
/label=Secretory (IgK)
/label=nonstandard type: signal
primer_bind 2739..2762
/label=olyl052
primer_bind 2739..2758
/label=oYS11
CDS 2826..3338
/codon_start=1
/product="NanoLuc(R) luciferase"
/label=Nluc
/note="human codon-optimized"
/translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
RLCERILA"
misc_feature 2859..2861
/label=cyt-STOP (TGG->TGA)
primer_bind complement(3321..3344)
/label=oYS12
primer_bind complement(3337..3397)
/label=olyl053
primer_bind complement(3337..3369)
/label=olyl055
primer_bind complement(3341..3398)
/label=olyl292
terminator 3345..3367
/label=SV40 late polyA
primer_bind complement(3400..3419)
/label=olyl293
primer_bind 3400..3419
/label=olyl294
polyA_signal 3415..3639
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(3626..3647)
/label=olyl286
primer_bind 3628..3648
/label=olyl287
rep_origin 3685..4113
/label=f1 bacteriophage origin of replication
/label=f1 bacteriophage origin of replication; arrow _f1
ori
primer_bind complement(3738..3757)
/label=f1 bac-phage-ori-rep-F
promoter 4127..4456
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
misc_feature 4359..4408
/label=P_SV40 TSS-region
misc_feature 4423..4428
/label=SDS
CDS 4523..5317
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 5491..5612
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5582..5614)
/label=olyl287
primer_bind complement(5657..5677)
/label=M13-rev
primer_bind complement(5661..5677)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_binding complement(5683..5705)
/label=LacO
protein_bind 5685..5701
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5709..5739)
/label=lac promoter
protein_bind 5754..5775
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 5881..6821
/label=ColE1
/note="ColE1"
CDS complement(6822..7682)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7683..7787)
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtattggat
241 ccaatgcatt ggatccaatg gattggatcc aatcgattgg atccaattga ttggatccaa
301 tctagctcga gctcggtacc cgggtcgagg taggcgtgta cggtgggagg cctatataag
361 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
421 ccatagaaga caccgggacc gatccagcct ccgcggcccc gaattcgagc tcgcccgggg
481 atcctcgcta gcgcttataa cttcgtatag catacattat acgaagttat cggcgcgccg
541 cggccgcgat caattcggta ccgaagttcc tattccgaat tcgcccacca tgctgctgct
601 gctgctgctg ctgggcctga ggctacagct ctccctgggc atcatcccag ttgaggagga
661 gaacccggac ttctggaacc gcgaggcagc cgaggccctg ggtgccgcca agaagctgca
721 gcctgcacag acagccgcca agaacctcat catcttcctg ggcgatggga tgggggtgtc
781 tacggtgaca gctgccagga tcctaaaagg gcagaagaag gacaaactgg ggcctgagat
841 acccctggcc atggaccgct tcccatatgt ggctctgtcc aagacataca atgtagacaa
901 acatgtgcca gacagtggag ccacagccac ggcctacctg tgcggggtca agggcaactt
961 ccagaccatt ggcttgagtg cagccgcccg ctttaaccag tgcaacacga cacgcggcaa
1021 cgaggtcatc tccgtgatga atcgggccaa gaaagcaggg aagtcagtgg gagtggtaac
1081 caccacacga gtgcagcacg cctcgccagc cggcacctac gcccacacgg tgaaccgcaa
1141 ctggtactcg gacgccgacg tgcctgcctc ggcccgccag gaggggtgcc aggacatcgc
1201 tacgcagctc atctccaaca tggacattga cgtgatccta ggtggaggcc gaaagtacat
1261 gtttcgcatg ggaaccccag accctgagta cccagatgac tacagccaag gtgggaccag
1321 gctggacggg aagaatctgg tgcaggaatg gctggcgaag cgccagggtg cccggtatgt
1381 gtggaaccgc actgagctca tgcaggcttc cctggacccg tctgtgaccc atctcatggg
1441 tctctttgag cctggagaca tgaaatacga gatccaccga gactccacac tggacccctc
1501 cctgatggag atgacagagg ctgccctgcg cctgctgagc aggaaccccc gcggcttctt
1561 cctcttcgtg gagggtggtc gcatcgacca tggtcatcat gaaagcaggg cttaccgggc
1621 actgactgag acgatcatgt tcgacgacgc cattgagagg gcgggccagc tcaccagcga
1681 ggaggacacg ctgagcctcg tcactgccga ccactcccac gtcttctcct tcggaggcta
1741 ccccctgcga gggagctcca tcttcgggct ggcccctggc aaggcccggg acaggaaggc
1801 ctacacggtc ctcctatacg gaaacggtcc aggctatgtg ctcaaggacg gcgcccggcc
1861 ggatgttacc gagagcgaga gcgggagccc cgagtatcgg cagcagtcag cagtgcccct
1921 ggacgaagag acccacgcag gcgaggacgt ggcggtgttc gcgcgcggcc cgcaggcgca
1981 cctggttcac ggcgtgcagg agcagacctt catagcgcac gtcatggcct tcgccgcctg
2041 cctggagccc tacaccgcct gcgacctggc gccccccgcc ggcaccaccg acgccgcgca
2101 cccgggttac tctagagtcg gggcggccgg ccgcttcgag cagacatgat ctagaggcct
2161 gcagggctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
2221 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
2281 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
2341 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
2401 tatggcttct gaggataaga tacattgatg agtttggaca aaccacaact agaatgcagt
2461 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
2521 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
2581 aggtgtggga ggttttttaa agcgcttggt accgtctttc cagtggttaa ttaaataact
2641 tcgtatagca tacattatac gaagttatgg atccgaacaa acgacccaac acccgtgcgt
2701 tttattctgt ctttttattg ccgatcctcg aggccaccat gactagtgag acagacacac
2761 tcctgctatg ggtactgctg ctctgggttc caggttccac tggtgacgct agtggtggtt
2821 ctggtatggt cttcacactc gaagatttcg ttggggactg gcgacagaca gccggctaca
2881 acctggacca agtccttgaa cagggaggtg tgtccagttt gtttcagaat ctcggggtgt
2941 ccgtaactcc gatccaaagg attgtcctga gcggtgaaaa tgggctgaag atcgacatcc
3001 atgtcatcat cccgtatgaa ggtctgagcg gcgaccaaat gggccagatc gaaaaaattt
3061 ttaaggtggt gtaccctgtg gatgatcatc actttaaggt gatcctgcac tatggcacac
3121 tggtaatcga cggggttacg ccgaacatga tcgactattt cggacggccg tatgaaggca
3181 tcgccgtgtt cgacggcaaa aagatcactg taacagggac cctgtggaac ggcaacaaaa
3241 ttatcgacga gcgcctgatc aaccccgacg gctccctgct gttccgagta accatcaacg
3301 gagtgaccgg ctggcggctg tgcgaacgca ttctggcgat cgatagacat gataagatac
3361 attgatgtct agaagcttct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc
3421 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
3481 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
3541 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
3601 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
3661 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
3721 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
3781 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
3841 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
3901 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
3961 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
4021 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
4081 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
4141 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
4201 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
4261 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
4321 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
4381 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
4441 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
4501 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
4561 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
4621 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
4681 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
4741 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
4801 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
4861 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
4921 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
4981 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
5041 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
5101 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
5161 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
5221 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
5281 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
5341 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
5401 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
5461 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
5521 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
5581 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
5641 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
5701 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
5761 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
5821 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
5881 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
5941 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
6001 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
6061 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
6121 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
6181 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
6241 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
6301 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
6361 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
6421 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
6481 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
6541 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
6601 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
6661 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
6721 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
6781 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
6841 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
6901 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
6961 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
7021 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
7081 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
7141 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
7201 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
7261 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
7321 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
7381 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
7441 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
7501 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
7561 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
7621 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
7681 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
7741 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
7801 aaagtgccac ctgacgtc
//
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