pDisplay-NE2h-IRES-mCherry-CAAX

LOCUS       pDisplay-NE2h-IRES-mCherry-CAAX              8537 bp DNA     circular SYN 23-JAN-2024
DEFINITION  Expresses the genetically-encoded fluorescent norepinephrine (NE) 
            sensor GRAB_NE2h and a membrane-localized mcherry in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    pDisplay-NE2h-IRES-mCherry-CAAX
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8537)
  TITLE     Monitoring norepinephrine release in vivo using next-generation 
            GRABNE sensors
REFERENCE   2  (bases 1 to 8537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 9, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     638..657
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             737..2728
                     /codon_start=1
                     /label=NE2h
                     /translation="METDTLLLWVLLLWVPGSTGDTSLYKKVGTGSLQPDAGNASWNGT
                     EAPGGGARATPYSLQVTLTLVCLAGLLMLLTVFGNVLVIIAVFTSRALKAPQNLFLVSL
                     ASADILVATLVIPFSLANEVMGYWYFGKAWCEIYLALDVLFCTSSIVHLCAISLDRYWS
                     ITQAKEYNLKRTPRRIKAIIITVWVISAVISFPPLISIEKKGGGGGPQPAEPRCEINDQ
                     KWYVISSCIGSFFAPCLIMILVYVRIYQIAKRRTRVPPSRRGPDAVAAPPGGTERRPNG
                     LGPERSAGPGGAEAEPLPTQLNGAPGEPAPAGPRDTDALDLEEGGNVYIKADKQKNGIK
                     ANFHIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKGGTGGSMVRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEG
                     DATEGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQ
                     ERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNTGAAARWRG
                     RQNREKRFIFVLAVVIGVFVVCWFPFFFTYTLTAVGCSVPRTLFKFFFWFGYCNSSLNP
                     VIYTIFNHDFRRAFKKILCRGDRKRIVL"
     sig_peptide     737..799
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1921..1942
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2048..2069)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2309..2328)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2736..3321
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2903..2920)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3129..3148
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3322..4029
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3467..3485)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3735..3754
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4087..4116
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4120..4266
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(4288..4305)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4294..4518
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4650..5238)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4730..4749)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5567..5614
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5639..5658
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5846..6640)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTYGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5957..5976)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6567..6586
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(6739..6758)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        6837..7028
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6985..7005
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(7088..7948)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7711..7730
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7949..8053)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8080..8535
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8167..8186)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8377..8398
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccctgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg
      721 cttggggata tccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt
      781 tccaggttcc actggtgaca caagtttgta caaaaaagtt ggcaccggct ccctgcagcc
      841 ggacgcgggc aacgcgagct ggaacgggac cgaggcgccg gggggcggcg cccgggccac
      901 cccttactcc ctgcaggtga cgctgacgct ggtgtgcctg gccggcctgc tcatgctgct
      961 caccgtgttc ggcaacgtgc tcgtcatcat cgccgtgttc acgagccgcg cgctcaaggc
     1021 gccccaaaac ctcttcctgg tgtctctggc ctcggccgac atcctggtgg ccacgctcgt
     1081 catccctttc tcgctggcca acgaggtcat gggctactgg tacttcggca aggcttggtg
     1141 cgagatctac ctggcgctcg acgtgctctt ctgcacgtcg tccatcgtgc acctgtgcgc
     1201 catcagcctg gaccgctact ggtccatcac acaggccaaa gagtacaacc tgaagcgcac
     1261 gccgcgccgc atcaaggcca tcatcatcac cgtgtgggtc atctcggccg tcatctcctt
     1321 cccgccgctc atctccatcg agaagaaggg cggcggcggc ggcccgcagc cggccgagcc
     1381 gcgctgcgag atcaacgacc agaagtggta cgtcatctcg tcgtgcatcg gctccttctt
     1441 cgctccctgc ctcatcatga tcctggtcta cgtgcgcatc taccagatcg ccaagcgtcg
     1501 cacccgcgtg ccacccagcc gccggggtcc ggacgccgtc gccgcgccgc cggggggcac
     1561 cgagcgcagg cccaacggtc tgggccccga gcgcagcgcg ggcccggggg gcgcagaggc
     1621 cgaaccgctg cccacccagc tcaacggcgc ccctggcgag cccgcgccgg ccgggccgcg
     1681 cgacaccgac gcgctggacc tggaggaggg aggaaacgtc tatatcaagg ccgacaagca
     1741 gaagaacggc atcaaggcga acttccacat ccgccacaac atcgaggacg gcggcgtgca
     1801 gctcgcctac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     1861 caaccactac ctgagcgtgc agtccaaact ttcgaaagac cccaacgaga agcgcgatca
     1921 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     1981 caagggcggt accggaggga gcatggtgag aaagggcgag gagctgttca ccggggtggt
     2041 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     2101 gggtgagggc gatgccaccg agggcaagct gaccctgaag ttcatctgca ccaccggcaa
     2161 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     2221 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     2281 catccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     2341 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     2401 ggacggcaac atcctggggc acaagctgga gtacaacacc ggagcagcag cacgctggcg
     2461 cgggcggcag aaccgcgaga agcgcttcat tttcgtgctg gccgtggtca tcggagtgtt
     2521 cgtggtgtgc tggttcccct tcttcttcac ctacacgctc acggccgtcg ggtgctccgt
     2581 gccacgcacg ctcttcaaat tcttcttctg gttcggctac tgcaacagct cgttgaaccc
     2641 ggtcatctac accatcttca accacgattt ccgccgcgcc ttcaagaaga tcctctgtcg
     2701 gggggacagg aagcggatcg tgttgtaagc tagcgcccct ctccctcccc cccccctaac
     2761 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     2821 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     2881 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     2941 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     3001 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaacgcca cgtgtataag
     3061 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     3121 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     3181 cccattgtat gggatctgat ctggggcctc ggtacacatg ctttacatgt gtttagtcga
     3241 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     3301 gatgataata tggccacaac catggtgagc aagggcgagg aggataacat ggccatcatc
     3361 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     3421 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     3481 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     3541 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     3601 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     3661 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     3721 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     3781 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     3841 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     3901 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     3961 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     4021 ctgtacaagg atggtaaaaa gaagaaaaag aagtcaaaga caaagtgtgt aattatgtaa
     4081 gtcgacgaac aaaaactcat ctcagaagag gatctgaatg ctgtgggcca ggacacgcag
     4141 gaggtcatcg tggtgccaca ctccttgccc tttaaggtgg tggtgatctc agccatcctg
     4201 gccctggtgg tgctcaccat catctccctt atcatcctca tcatgctttg gcagaagaag
     4261 ccacgttagg cggccgctcg agatcagcct cgactgtgcc ttctagttgc cagccatctg
     4321 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     4381 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     4441 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     4501 atgcggtggg ctctatggct tctgaggcgg aaagaaccag tggcggtaat acggttatcc
     4561 acagaatcgg gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     4621 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     4681 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     4741 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4801 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4861 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4921 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacgc
     4981 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5041 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     5101 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     5161 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5221 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     5281 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     5341 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaacctgag
     5401 gctatggcag ggcctgccgc cccgacgttg gctgcgagcc ctgggccttc acccgaactt
     5461 ggggggtggg gtggggaaaa ggaagaaacg cgggcgtatt ggccccaatg gggtctcggt
     5521 ggggtatcga cagagtgcca gccctgggac cgaaccccgc gtttatgaac aaacgaccca
     5581 acaccgtgcg ttttattctg tctttttatt gccgtcatag cgcgggttcc ttccggtatt
     5641 gtctccttcc gtgtttcagt tagcctcccc ctagggtggg cgaagaactc cagcatgaga
     5701 tccccgcgct ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt
     5761 tcatagaagg cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg
     5821 gtcatttcga accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga
     5881 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc
     5941 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca
     6001 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg
     6061 gcaagcaggc atcgccatag gtcacgacga gatcctcgcc gtcgggcatg ctcgccttga
     6121 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat
     6181 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt
     6241 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg
     6301 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca
     6361 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc
     6421 ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg cagttcattc agggcaccgg
     6481 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg
     6541 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag
     6601 cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg
     6661 tctcttgatc gatctttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg
     6721 aatagctcag aggccgggcc tcggcctctg cataaataaa aaaaattagt cagccatggg
     6781 gcggagaatg ggcggaactg ggcggagtta ggggcggaat gggcggagtt aggggcggga
     6841 ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg
     6901 gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
     6961 ctggggagcc tggggacttt ccacacccta actgacacac attccacagc tggttctttc
     7021 cgcctcagga ctcttccttt ttcaataaat caatctaaag tatatatgag taaacttggt
     7081 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7141 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7201 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7261 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7321 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7381 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7441 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7501 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7561 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7621 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7681 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7741 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7801 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7861 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     7921 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     7981 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8041 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
     8101 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     8161 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     8221 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     8281 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     8341 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     8401 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     8461 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     8521 taacgcttac aatttac
//