pQL90

LOCUS       pQL90              5257 bp DNA     circular SYN 07-APR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Jun 22, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5257
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     primer_bind     172..213
                     /label=QL408-F
     regulatory      193..475
                     /note="DHP; the nucleotide coordinates are approximate 
                     forthis feature"
                     /db_xref="GeneID:111832672"
                     /experiment="EXISTENCE:in vivo cleavage assay evidence 
                     [ECO:0001075][PMID:2458988]"
                     /regulatory_class="DNase_I_hypersensitive_site"
     regulatory      193..475
                     /function="may be silencer in fibroblasts"
                     /note="HAF; NlaIV fragment"
                     /db_xref="GeneID:111832672"
                     /experiment="EXISTENCE:reporter gene assay evidence 
                     [ECO:0000049][PMID:2471155]"
                     /regulatory_class="silencer"
     regulatory      193..475
                     /function="enhancer in hepatocellular carcimona cells"
                     /note="HAF; NlaIV fragment"
                     /db_xref="GeneID:111832672"
                     /experiment="EXISTENCE:reporter gene assay evidence 
                     [ECO:0000049][PMID:2471155]"
                     /regulatory_class="enhancer"
     regulatory      193..475
                     /label=1 kb promoter
                     /note="1 kb promoter"
                     /db_xref="GeneID:111832672"
                     /experiment="EXISTENCE:reporter gene assay evidence 
                     [ECO:0000049][PMID:1373328]"
                     /regulatory_class="promoter"
     misc_feature    193..475
                     /standard_name="alpha fetoprotein (Afp) 5' 
                     regulatoryregion"
                     /function="regulatory_interactions: Afp | Alb"
                     /label=alpha fetoprotein (Afp) 5' regulatory
                     region
                     /note="Region: biological region; Derived by automated 
                     computational analysis using gene prediction method: 
                     RefSeqFE."
                     /db_xref="GeneID:111832672"
     regulatory      193..305
                     /function="mediates repression by forkhead box A"
                     /note="A fragment; -250/-137"
                     /db_xref="GeneID:111832672"
                     /experiment="EXISTENCE:reporter gene assay evidence 
                     [ECO:0000049][PMID:12215259]"
                     /regulatory_class="silencer"
     regulatory      240..363
                     /label=-203 to -79 fragment
                     /note="-203 to -79 fragment"
                     /db_xref="GeneID:111832672"
                     /experiment="EXISTENCE:reporter gene assay evidence 
                     [ECO:0000049][PMID:1700853]"
                     /regulatory_class="enhancer"
     protein_bind    264..296
                     /label=hepatocyte nuclear factor 4 alpha binding site
                     /bound_moiety="hepatocyte nuclear factor 4 alpha"
                     /note="DR-1; HNF4 site D"
                     /db_xref="GeneID:111832672"
                     /experiment="EXISTENCE:protein binding evidence 
                     [ECO:0000024][PMID:11549270]"
     protein_bind    271..283
                     /label=nuclear receptor subfamily 3 group C member
                     1 b
                     /bound_moiety="nuclear receptor subfamily 3 group C 
                     member1"
                     /note="HRE or GRE region IV"
                     /db_xref="GeneID:111832672"
                     /experiment="EXISTENCE:protein binding evidence 
                     [ECO:0000024][PMID:7517187]"
     regulatory      291..389
                     /function="mediates repression by ZBTB20"
                     /label=-151 to -53 fragment
                     /note="-151 to -53 fragment"
                     /db_xref="GeneID:111832672"
                     /experiment="EXISTENCE:reporter gene assay evidence 
                     [ECO:0000049][PMID:26173901]"
                     /regulatory_class="silencer"
     regulatory      297..337
                     /label=EP2
                     /note="EP2"
                     /db_xref="GeneID:111832672"
                     /experiment="EXISTENCE:reporter gene assay evidence 
                     [ECO:0000049][PMID:1378295]"
                     /regulatory_class="enhancer"
     protein_bind    297..337
                     /label=CCAAT/enhancer binding protein alpha binding site
                     /bound_moiety="CCAAT/enhancer binding protein alpha"
                     /note="EP2 C/EBP site"
                     /db_xref="GeneID:111832672"
                     /experiment="EXISTENCE:protein binding evidence 
                     [ECO:0000024][PMID:1378295]"
     protein_bind    310..346
                     /label=HNF1 homeobox A binding site
                     /bound_moiety="HNF1 homeobox A"
                     /note="HNF1 -118 site"
                     /db_xref="GeneID:111832672"
                     /experiment="EXISTENCE:protein binding evidence 
                     [ECO:0000024][PMID:2479822]"
     protein_bind    338..356
                     /function="negative regulation of promoter activity"
                     /label=bindin
                     /bound_moiety="zinc finger and BTB domain containing 20"
                     /note="ZBT20 site"
                     /db_xref="GeneID:111832672"
                     /experiment="EXISTENCE:protein binding evidence 
                     [ECO:0000024][PMID:26173901]"
     protein_bind    362..404
                     /function="negative regulation of promoter activity"
                     /label=thyroid hormone receptor binding site
                     /bound_moiety="thyroid hormone receptor"
                     /note="DR4"
                     /db_xref="GeneID:111832672"
                     /experiment="EXISTENCE:protein binding evidence 
                     [ECO:0000024][PMID:9484909]"
     protein_bind    362..404
                     /label=forkhead box A1, A2, and A3 binding site
                     /bound_moiety="forkhead box A1, A2, and A3"
                     /note="DR4"
                     /db_xref="GeneID:111832672"
                     /experiment="EXISTENCE:protein binding evidence 
                     [ECO:0000024][PMID:11911950]"
     protein_bind    384..404
                     /label=X-ray repair cross complementing 6 binding site
                     /bound_moiety="X-ray repair cross complementing 6"
                     /note="-58 to -38 Ku binding site; when adopted 
                     structuredform"
                     /db_xref="GeneID:111832672"
                     /experiment="EXISTENCE:protein binding evidence 
                     [ECO:0000024][PMID:16765502]"
     regulatory      412..418
                     /db_xref="GeneID:111832672"
                     /experiment="COORDINATES:motif similarity evidence 
                     [ECO:0000028][PMID:6193415]"
                     /regulatory_class="TATA_box"
     mRNA            419..475
                     /product="alpha fetoprotein"
                     /label=alpha fetoprotein mRNA
                     /note="Derived by automated computational analysis 
                     usinggene prediction method: BestRefSeq."
                     /note="/transcript_id=NM_007423.4"
                     /db_xref="GeneID:11576"
     gene            419..475
                     /gene="Afp"
                     /label=Afp
                     /note="alpha fetoprotein; Derived by automatedcomputational
                     analysis using gene prediction method: BestRefSeq."
                     /db_xref="GeneID:11576"
                     /db_xref="MGI:MGI:87951"
     primer_bind     complement(455..495)
                     /label=QL409-R
     regulatory      492..501
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             498..2150
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2165..2389
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2407..2547
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2407..2536
                     /label=AAV2 ITR
     primer_bind     complement(2605..2621)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2629..2645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2653..2683)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2698..2719
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3007..3595)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3766..4626)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4627..4731)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5178..5201
                     /label=M13F-47
     primer_bind     5205..5221
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct cgacaattgc atgaaggcga tgtacgggcc agatatacgc gtaatctgct
      181 tagggcggcc gcaggggaaa taatctattt gaacagttta gatggtgaag aacatttgca
      241 gcatttgcaa gatttttttc cactctgaag tggtctttgt ccttgaacat aggatacaag
      301 tgacccctgc tctgttaatt attggcaaat tgcctaactt caacgtaagg aaatagagtc
      361 atatgtttgc tcactgaagg ttactagtta acaggcatcc cttaaacagg atataaaagg
      421 acttcagcag gactgctcga aacatcccac ttccagcact gcctgcggtg aaggagctag
      481 cgctaccggt cgccaccatg gaagatgcca aaaacattaa gaagggccca gcgccattct
      541 acccactcga agacgggacc gccggcgagc agctgcacaa agccatgaag cgctacgccc
      601 tggtgcccgg caccatcgcc tttaccgacg cacatatcga ggtggacatt acctacgccg
      661 agtacttcga gatgagcgtt cggctggcag aagctatgaa gcgctatggg ctgaatacaa
      721 accatcggat cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc gtgttgggtg
      781 ccctgttcat cggtgtggct gtggccccag ctaacgacat ctacaacgag cgcgagctgc
      841 tgaacagcat gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa
      901 agatcctcaa cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc atggatagca
      961 agaccgacta ccagggcttc caaagcatgt acaccttcgt gacttcccat ttgccacccg
     1021 gcttcaacga gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga
     1081 tcatgaacag tagtggcagt accggattgc ccaagggcgt agccctaccg caccgcaccg
     1141 cttgtgtccg attcagtcat gcccgcgacc ccatcttcgg caaccagatc atccccgaca
     1201 ccgctatcct cagcgtggtg ccatttcacc acggcttcgg catgttcacc acgctgggct
     1261 acttgatctg cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc
     1321 gcagcttgca agactataag attcaatctg ccctgctggt gcccacacta tttagcttct
     1381 tcgctaagag cactctcatc gacaagtacg acctaagcaa cttgcacgag atcgccagcg
     1441 gcggggcgcc gctcagcaag gaggtaggtg aggccgtggc caaacgcttc cacctaccag
     1501 gcatccgcca gggctacggc ctgacagaaa caaccagcgc cattctgatc acccccgaag
     1561 gggacgacaa gcctggcgca gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg
     1621 acttggacac cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc
     1681 ccatgatcat gagcggctac gttaacaacc ccgaggctac aaacgctctc atcgacaagg
     1741 acggctggct gcacagcggc gacatcgcct actgggacga ggacgagcac ttcttcatcg
     1801 tggaccggct gaagagcctg atcaaataca agggctacca ggtagcccca gccgaactgg
     1861 agagcatcct gctgcaacac cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg
     1921 acgatgccgg cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa accatgaccg
     1981 agaaggagat cgtggactat gtggccagcc aggttacaac cgccaagaag ctgcgcggtg
     2041 gtgttgtgtt cgtggacgag gtgcctaaag gactgaccgg caagttggac gcccgcaaga
     2101 tccgcgagat tctcattaag gccaagaagg gcggcaagat cgccgtgtaa gaattctgcc
     2161 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     2221 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     2281 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     2341 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggg gcctcgagcg
     2401 cgtgtgagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
     2461 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
     2521 gcgagcgagc gcgcagctgc ctgcaggatc ggatgccggg accgacgagt gcagaggcgt
     2581 gcaagcgagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     2641 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     2701 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     2761 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     2821 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     2881 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     2941 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3001 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3061 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3121 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3181 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3241 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3301 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3361 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     3421 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     3481 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3541 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3601 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3661 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3721 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     3781 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     3841 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     3901 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     3961 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     4021 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     4081 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     4141 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     4201 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     4261 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     4321 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     4381 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     4441 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     4501 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     4561 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     4621 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     4681 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     4741 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     4801 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     4861 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     4921 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     4981 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     5041 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
     5101 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     5161 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     5221 tgaattggag atcggtactt cgcgaatgcg tcgagat
//