PCDH-TetO-mEGFP-TRIM9-TurboID

LOCUS       PCDH-TetO-mEGFP-TRIM9-TurboID              10307 bp DNA     circular SYN 31-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10307)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 12, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1980..2250
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2283..2320
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2394..2440
                     /label=Primer 2
     primer_bind     2414..2448
                     /label=Primer 1a
     CDS             2444..3160
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      2444..2447
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2489..2510)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2750..2769)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3097..3118
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3161..3190
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     complement(3171..3196)
                     /label=Primer 2a
     primer_bind     3172..3214
                     /label=mcherry-TRIM9-F
     misc_feature    3197..5326
                     /label=TRIM9 long
     primer_bind     complement(3670..3680)
                     /label=mcherry-TRIM9-R
     primer_bind     complement(3671..3680)
                     /label=GFP-TRIM9-Turboid-R
     primer_bind     3726..3743
                     /label=T9-seqF1
     primer_bind     4477..4492
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5307..5326)
                     /label=mcherry-TRIM9-R
     primer_bind     complement(5308..5346)
                     /label=GFP-TRIM9-Turboid-R
     primer_bind     complement(5327..5348)
                     /label=Primer 4
     CDS             5333..5359
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5363..5389
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5393..5419
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5420..6400
                     /codon_start=1
                     /label=TurboID
                     /translation="ASKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKS
                     GDACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEKEFSRAD"
     primer_bind     complement(5981..5997)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    6404..6991
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6404..6419)
                     /label=Primer 1
     primer_bind     complement(6404..6419)
                     /label=mcherry-TRIM9-R
     primer_bind     complement(6404..6419)
                     /label=TRIM9s-R
     primer_bind     complement(6404..6419)
                     /label=Primer 2a
     CDS             complement(6875..6886)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7064..7297
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7369..7490
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      7509..7644
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7682..7698)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7706..7722
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7730..7760)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7775..7796
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8084..8672)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8843..9703)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9704..9808)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10177..10190)
                     /label=Primer 2
     primer_bind     10282..10298
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtcgg gtttattaca gggacagcag agatccagtt tggttatcga gtttaccact
     1981 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2041 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     2101 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     2161 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2221 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc
     2281 gagtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat
     2341 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
     2401 cctccgcggc ccctctagac cgctagcgct accggtcgcc accatggtga gcaagggcga
     2461 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     2521 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     2581 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     2641 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     2701 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     2761 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     2821 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     2881 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     2941 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     3001 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     3061 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     3121 cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca gatctcgagc
     3181 tcaagcttcg gaattcatgg aggagatgga agaggagttg aaatgccccg tgtgcggctc
     3241 cttctatcgg gagcccatca tcctgccctg ctctcacaat ttgtgtcagg cgtgcgcccg
     3301 caacatcctg gtgcagaccc cagagtctga atccccccag agccatcggg ccgcgggctc
     3361 cggggtctcc gactatgact atctggacct ggacaagatg agcctataca gcgaggcgga
     3421 cagcggctat ggctcctacg gggggttcgc cagcgccccc actaccccgt gccagaagtc
     3481 ccccaacggc gtccgcgtgt ttcccccggc tatgccgcca ccggccaccc acttgtcacc
     3541 ggccctggcc ccggtgcccc gcaactcctg tatcacctgc ccccagtgtc accgcagcct
     3601 catcctggat gaccgggggc tccgcggctt ccccaagaat cgcgtactgg aaggggtaat
     3661 tgaccgctac cagcagagca aagccgcggc cctcaagtgc cagctctgcg agaaggcgcc
     3721 caaggaagcc accgtcatgt gcgaacagtg cgatgtcttc tactgcgatc cgtgccgcct
     3781 gcgctgccac ccgccccggg ggcccctagc caagcaccgc ctggtgcccc cggcccaggg
     3841 tcgtgtgagc cggaggctga gcccacgcaa ggtctccacc tgcacagacc acgagctgga
     3901 gaaccacagc atgtactgcg tgcaatgcaa gatgcccgtg tgctaccagt gcttggagga
     3961 gggcaaacac tccagccacg aagtcaaggc tctgggggcc atgtggaaac tacataagag
     4021 ccagctctcc caggcgctga acggactgtc agacagggcc aaagaagcca aggagtttct
     4081 ggtacagctg cgcaacatgg tccagcagat ccaggagaac agtgtggagt ttgaagcctg
     4141 tctggtggcc caatgtgatg ccctcatcga tgccctcaac agaagaaaag cccagctgct
     4201 ggcccgcgtc aacaaggagc atgagcacaa gctgaaggtg gttcgagatc agatctctca
     4261 ctgcacagtg aaattgcgcc agaccacagg tctcatggag tactgcttgg aggtgattaa
     4321 ggaaaatgat cctagtggtt ttttgcagat ttctgacgcc ctcataagaa gagtgcacct
     4381 gactgaggat cagtggggta aaggcacact cactccaagg atgaccacgg actttgactt
     4441 gagtctggac aacagccctc tgctgcaatc catccaccag ctggatttcg tgcaagtgaa
     4501 agcttcctct ccagtcccag caacccctat cctacagctg gaggaatgtt gtacccacaa
     4561 caacagcgct acgttgtcct ggaaacagcc acctctgtcc acggtgcccg ccgatggata
     4621 cattctggag ctggatgatg gcaacggtgg tcaattccgg gaggtgtatg tggggaagga
     4681 gacaatgtgc actgtggatg gtcttcactt caacagcaca tacaacgctc gggtcaaggc
     4741 cttcaacaaa acaggagtca gcccgtacag caagaccctg gtcctccaaa cgtctgaggt
     4801 ggcctggttt gctttcgacc ctggctcggc gcactcggac atcatcctct ccaatgacaa
     4861 cctgacagtg acctgtagta gctatgatga ccgggtggtg ctagggaaga ctggcttctc
     4921 caagggcatc cactactggg agctcacggt agatcgctat gacaaccacc ctgatcctgc
     4981 ctttggtgtg gctcgcatgg acgtgatgaa ggatgtgatg ttaggaaaag acgacaaagc
     5041 ttgggcaatg tatgtggaca ataaccggag ctggttcatg cacaacaact cgcacaccaa
     5101 cagaactgag ggagggatca caaaaggggc cacaattggg gtcctcctcg acttaaatag
     5161 aaaaaacttg acatttttta tcaacgatga acaacaaggt cccatagcat ttgataacgt
     5221 ggagggcctc ttcttccctg cggtcagcct gaacaggaac gtgcaggtca cgctgcacac
     5281 cgggctccca gtccccgact tctactccag cagagcatca atagccggat cctacccgta
     5341 tgatgttccg gattacgctg gctatcccta cgacgtgccc gactatgccg ggtaccccta
     5401 tgacgtccca gactacgcag ctagcaaaga caatactgtg cctctgaagc tgatcgctct
     5461 cctggctaat ggcgagttcc atagtggcga acagctggga gaaaccctgg gcatgtccag
     5521 ggccgctatc aacaagcaca ttcagactct gcgcgactgg ggcgtggacg tgttcaccgt
     5581 gcccggaaag ggctactctc tgcccgagcc tatcccgctg ctgaacgcta aacagattct
     5641 gggacagctg gacggcggga gcgtggcagt cctgcctgtg gtcgactcca ccaatcagta
     5701 cctgctggat cgaatcggcg agctgaagag tggggatgct tgcattgcag aatatcagca
     5761 ggcagggaga ggaagcagag ggaggaaatg gttctctcct tttggagcta acctgtacct
     5821 gagtatgttt tggcgcctga agcggggacc agcagcaatc ggcctgggcc cggtcatcgg
     5881 aattgtcatg gcagaagcgc tgcgaaagct gggagcagac aaggtgcgag tcaaatggcc
     5941 caatgacctg tatctgcagg atagaaagct ggcaggcatc ctggtggagc tggccggaat
     6001 aacaggcgat gctgcacaga tcgtcattgg cgccgggatt aacgtggcta tgaggcgcgt
     6061 ggaggaaagc gtggtcaatc agggctggat cacactgcag gaagcaggga ttaacctgga
     6121 caggaatact ctggccgcta cgctgatccg agagctgcgg gcagccctgg aactgttcga
     6181 gcaggaaggc ctggctccat atctgccacg gtgggagaag ctggataact tcatcaatag
     6241 acccgtgaag ctgatcattg gggacaaaga gattttcggg attagccggg ggattgataa
     6301 acagggagcc ctgctgctgg aacaggacgg agttatcaaa ccctggatgg gcggagaaat
     6361 cagtctgcgg tctgccgaaa aggagttcag cagggccgac tgaaatcaac ctctggatta
     6421 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     6481 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     6541 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     6601 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     6661 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     6721 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     6781 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     6841 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     6901 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt ctccgccttc gccctcagac
     6961 gagtcggatc tccctttggc cgcctccccg ccggtacctt taagaccaat gacttacaag
     7021 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7081 tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     7141 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7201 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7261 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     7321 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     7381 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7441 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     7501 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7561 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     7621 agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat
     7681 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7741 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7801 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7861 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7921 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7981 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8041 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8101 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8161 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8221 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8281 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8341 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8401 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8461 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8521 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8581 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8641 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8701 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8761 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8821 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8881 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8941 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9001 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9061 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9121 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9181 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9241 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9301 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9361 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9421 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9481 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9541 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9601 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9661 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9721 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9781 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     9841 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     9901 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     9961 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
    10021 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
    10081 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
    10141 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
    10201 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
    10261 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg
//