PCDH-TetO-mEGFP-TRIM45-TurboID

LOCUS       PCDH-TetO-mEGFP-TRIM45-TurboID               9917 bp DNA     circular SYN 04-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9917)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 12, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1980..2250
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2283..2320
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             2444..3160
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      2444..2447
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3161..3190
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    3197..4936
                     /label=TRIM45
     CDS             4943..4969
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4973..4999
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5003..5029
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5030..6010
                     /codon_start=1
                     /label=TurboID
                     /translation="ASKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKS
                     GDACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEKEFSRAD"
     misc_feature    6014..6601
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6485..6496)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6674..6907
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6979..7100
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      7119..7254
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7292..7308)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7316..7332
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7340..7370)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7385..7406
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7694..8282)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8453..9313)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9314..9418)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9892..9908
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtcgg gtttattaca gggacagcag agatccagtt tggttatcga gtttaccact
     1981 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2041 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     2101 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     2161 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2221 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc
     2281 gagtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat
     2341 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
     2401 cctccgcggc ccctctagac cgctagcgct accggtcgcc accatggtga gcaagggcga
     2461 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     2521 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     2581 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     2641 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     2701 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     2761 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     2821 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     2881 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     2941 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     3001 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     3061 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     3121 cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca gatctcgagc
     3181 tcaagcttcg gaattcatgt cagaaaacag aaaaccgctg ctgggctttg taagcaaact
     3241 cactagtggg actgcacttg ggaactcagg caagactcac tgccccctgt gcttggggct
     3301 tttcaaagcc cccaggctct tgccttgttt gcatacagtt tgcaccacgt gtctggagca
     3361 gctggagccc ttctcagtag tggacatccg agggggagac tctgacacaa gctctgaggg
     3421 gtcaatattc caggaactca agccacgaag tctgcagtcg cagatcggca tcctttgtcc
     3481 tgtatgtgat gctcaggtgg acctgcccat gggtggagtg aaggctttaa ccatagacca
     3541 cctggccgtg aatgatgtga tgctggagag cctacgtggg gaaggccagg gcctggtgtg
     3601 tgacctgtgc aacgacaggg aagtagagaa gaggtgtcag acctgcaaag ccaacctctg
     3661 ccacttctgc tgccaggctc ataggcggca gaagaaaacg acttaccaca ccatggtgga
     3721 cctaaaagac ttgaaaggct acagccggat tgggaagccc atcctgtgtc ctgttcaccc
     3781 tgcagaggaa ctgaggctgt tctgtgagtt ctgtgaccgg cccgtgtgcc aggattgtgt
     3841 ggtgggggag catcgggaac acccctgtga cttcaccagc aatgtcatcc acaagcatgg
     3901 ggactctgtg tgggagctcc tcaaaggtac tcagccccac gtggaggccc tggaggaagc
     3961 cctggctcag atccacataa taaacagtgc cctccagaag cgagtggagg cagtggcagc
     4021 tgatgtccgg acattctcgg agggctacat taaggccatt gaggagcatc gggacaagct
     4081 gctgaagcag ctggaagaca tacgggccca gaaggaaaat tccctgcagc tgcagaaggc
     4141 ccagctggaa cagttactgg cagacatgcg gactggagtg gagttcaccg agcacttgct
     4201 gaccagcggc tcagacttgg agatcctcat caccaagagg gtggtggtag aacggctcag
     4261 gaagctgaac aaagttcaat atagcacccg tcctggagta aatgataaga tacgcttctg
     4321 tcctcaggag aaagcaggcc agtgccgtgg ctatgaaatt tatggtacga ttaataccaa
     4381 agaggttgat ccagccaaat gtgtcctaca aggagaagac ctccacagag cccgggagaa
     4441 acagacggcc tctttcaccc tgctttgtaa ggatgccgca ggagaaatca tgggcagggg
     4501 aggagacaac gttcaagttg ccgttgtccc taaagataag aaagacagcc cagtcagaac
     4561 aatggtccag gataacaagg atgggacata ctacatttcc tacaccccca aggaacctgg
     4621 cgtctatact gtgtgggtct gcatcaaaga acagcatgtg cagggctcgc cattcactgt
     4681 gatggtgagg agaaagcacc gcccacactc aggcgtgttt cactgctgca ccttctgctc
     4741 cagcgggggc cagaaaaccg ctcgctgcgc ctgtggaggc accatgccag gtgggtacct
     4801 aggctgtggc catggacaca aaggccaccc aggtcatccc cactggtcat gctgtggaaa
     4861 atttaatgag aaatctgaat gcacatggac aggtgggcag agcgcaccga ggagtctact
     4921 taggactgtg gctctcggat cctacccgta tgatgttccg gattacgctg gctatcccta
     4981 cgacgtgccc gactatgccg ggtaccccta tgacgtccca gactacgcag ctagcaaaga
     5041 caatactgtg cctctgaagc tgatcgctct cctggctaat ggcgagttcc atagtggcga
     5101 acagctggga gaaaccctgg gcatgtccag ggccgctatc aacaagcaca ttcagactct
     5161 gcgcgactgg ggcgtggacg tgttcaccgt gcccggaaag ggctactctc tgcccgagcc
     5221 tatcccgctg ctgaacgcta aacagattct gggacagctg gacggcggga gcgtggcagt
     5281 cctgcctgtg gtcgactcca ccaatcagta cctgctggat cgaatcggcg agctgaagag
     5341 tggggatgct tgcattgcag aatatcagca ggcagggaga ggaagcagag ggaggaaatg
     5401 gttctctcct tttggagcta acctgtacct gagtatgttt tggcgcctga agcggggacc
     5461 agcagcaatc ggcctgggcc cggtcatcgg aattgtcatg gcagaagcgc tgcgaaagct
     5521 gggagcagac aaggtgcgag tcaaatggcc caatgacctg tatctgcagg atagaaagct
     5581 ggcaggcatc ctggtggagc tggccggaat aacaggcgat gctgcacaga tcgtcattgg
     5641 cgccgggatt aacgtggcta tgaggcgcgt ggaggaaagc gtggtcaatc agggctggat
     5701 cacactgcag gaagcaggga ttaacctgga caggaatact ctggccgcta cgctgatccg
     5761 agagctgcgg gcagccctgg aactgttcga gcaggaaggc ctggctccat atctgccacg
     5821 gtgggagaag ctggataact tcatcaatag acccgtgaag ctgatcattg gggacaaaga
     5881 gattttcggg attagccggg ggattgataa acagggagcc ctgctgctgg aacaggacgg
     5941 agttatcaaa ccctggatgg gcggagaaat cagtctgcgg tctgccgaaa aggagttcag
     6001 cagggccgac tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     6061 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     6121 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     6181 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     6241 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     6301 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     6361 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     6421 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     6481 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     6541 tcttccgcgt ctccgccttc gccctcagac gagtcggatc tccctttggc cgcctccccg
     6601 ccggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     6661 gaaaaggggg gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct
     6721 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6781 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6841 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6901 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     6961 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7021 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7081 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     7141 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7201 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact
     7261 tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7321 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7381 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7441 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7501 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7561 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7621 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7681 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7741 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7801 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7861 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7921 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7981 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8041 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8101 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     8161 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8221 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8281 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8341 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8401 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8461 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8521 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8581 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8641 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8701 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8761 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8821 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8881 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8941 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9001 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9061 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9121 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9181 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9241 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9301 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9361 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9421 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     9481 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     9541 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     9601 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     9661 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     9721 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
     9781 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     9841 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     9901 cggccagtgc caagctg
//