PCDH-CMV-mCherry-TRIM23

LOCUS       PCDH-CMV-mCherry-TRIM23                8874 bp DNA     circular SYN 04-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8874)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1904..1953
                     /label=Primer 4
     enhancer        1938..2241
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2242..2445
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2444..2491)
                     /label=Primer 1
     primer_bind     2466..2500
                     /label=Primer 2
     primer_bind     2466..2492
                     /label=Primer 5
     CDS             2496..3203
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3025..3046
                     /label=mcherry-F
     misc_feature    3204..3232
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     3220..3258
                     /label=TRIM23-F
     misc_feature    3240..4964
                     /label=TRIM23
     primer_bind     complement(4945..4983)
                     /label=TRIM23-R
     misc_feature    4971..5558
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5442..5453)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5631..5864
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5936..6057
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6076..6211
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6249..6265)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6273..6289
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6297..6327)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6342..6363
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6651..7239)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7410..8270)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8271..8375)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8744..8757)
                     /label=Primer 5
     primer_bind     8849..8865
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagttgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2461 cagattctag accgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggata
     2521 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     2581 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     2641 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     2701 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     2761 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2821 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2881 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2941 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     3001 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     3061 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     3121 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     3181 gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcggaattca
     3241 tggctaccct ggttgtaaac aagctcggag cgggagtaga cagtggccgg cagggcagcc
     3301 gggggacagc tgtagtgaag gtgctagagt gtggagtttg tgaagatgtc ttttctttgc
     3361 aaggagacaa agttccccgt cttttgcttt gtggccatac cgtctgtcat gactgtctca
     3421 ctcgcctacc tcttcatgga agagcaatcc gttgcccatt tgatcgacaa gtaacagacc
     3481 taggtgattc aggtgtctgg ggattgaaaa aaaattttgc tttattggag cttttggaac
     3541 gactgcagaa tgggcctatt ggtcagtatg gagctgcaga agaatccatt gggatatctg
     3601 gagagagcat cattcgttgt gatgaagatg aagctcacct tgcctctgta tattgcactg
     3661 tgtgtgcaac tcatttgtgc tctgagtgtt ctcaagttac tcattctaca aagacattag
     3721 caaagcacag gcgagttcct ctagctgata aacctcatga gaaaactatg tgctctcagc
     3781 accaggtgca tgccattgag tttgtttgct tggaagaagg ttgtcaaact agcccactca
     3841 tgtgctgtgt ctgcaaagaa tatggaaaac accagggtca caagcattca gtattggaac
     3901 cagaagctaa tcagatccga gcatcaattt tagatatggc tcactgcata cggaccttca
     3961 cagaggaaat ctcagattat tccagaaaat tagttggaat tgtgcagcac attgaaggag
     4021 gagaacaaat cgtggaagat ggaattggaa tggctcacac agaacatgta ccagggactg
     4081 cagagaatgc ccggtcatgt attcgagctt atttttatga tctacatgaa actctgtgtc
     4141 gtcaagaaga aatggctcta agtgttgttg atgctcatgt tcgtgaaaaa ttgatttggc
     4201 tcaggcagca acaagaagat atgactattt tgttgtcaga ggtttctgca gcctgcctcc
     4261 actgtgaaaa gactttgcag caggatgatt gtagagttgt cttggcaaaa caggaaatta
     4321 caaggttact ggaaacattg cagaaacagc agcagcagtt tacagaagtt gcagatcaca
     4381 ttcagttgga tgccagcatc cctgtcactt ttacaaagga taatcgagtt cacattggac
     4441 caaaaatgga aattcgggtc gttacgttag gattggatgg tgctggaaaa actactatct
     4501 tgtttaagtt aaaacaggat gaattcatgc agcccattcc aacaattggt tttaacgtgg
     4561 aaactgtaga atataaaaat ctaaaattca ctatttggga tgtaggtgga aaacacaaat
     4621 taagaccatt gtggaaacat tattacctca atactcaagc tgttgtgttt gttgtagata
     4681 gcagtcatag agacagaatt agtgaagcac acagcgaact tgcaaagttg ttaacggaaa
     4741 aagaactccg agatgctctg ctcctgattt ttgctaacaa acaggatgtt gctggagcac
     4801 tgtcagtaga agaaatcact gaactactca gtctccataa attatgctgt ggccgtagct
     4861 ggtatattca gggctgtgat gctcgaagtg gtatgggact gtatgaaggg ttggactggc
     4921 tctcacggca acttgtagct gctggagtat tggatgttgc ttgaggatcc aatcaacctc
     4981 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     5041 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     5101 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     5161 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     5221 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     5281 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     5341 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     5401 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     5461 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc cgccttcgcc
     5521 ctcagacgag tcggatctcc ctttggccgc ctccccgccg gtacctttaa gaccaatgac
     5581 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     5641 aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga
     5701 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     5761 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     5821 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     5881 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     5941 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6001 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6061 tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6121 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     6181 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg
     6241 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6301 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6361 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6421 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6481 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6541 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6601 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6661 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6721 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6781 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6841 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6901 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6961 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7021 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7081 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7141 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7201 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7261 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7321 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7381 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7441 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7501 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7561 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7621 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7681 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7741 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7801 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7861 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7921 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7981 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8041 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8101 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8161 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8221 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8281 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8341 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8401 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     8461 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     8521 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     8581 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     8641 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     8701 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     8761 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     8821 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg
//