pAAVLINKv1-pCALM1-Cre-3'-PHF3-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-3'-PHF3-FLAG             6964 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6964)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 9, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3677
                     /codon_start=1
                     /gene="PHF3"
                     /product="PHD finger protein 3 isoform 1"
                     /label=PHF3
                     /note="isoform 1 is encoded by transcript variant 4"
                     /db_xref="GI:1634229891"
                     /db_xref="CCDS:CCDS4966.1"
                     /db_xref="GeneID:23469"
                     /db_xref="HGNC:HGNC:8921"
                     /db_xref="MIM:607789"
                     /protein_id="NP_001357277.1"
                     /translation="VKDMYLIPLGATDKIPHPLVPFDGPGLELHRPNLLLGLIIRQKLK
                     RQHSACASTSHIAETPESAPPIALPPDKKSKIEVSTEEAPEEENDFFNSFTTVLHKQRN
                     KPQQNLQEDLPTAVEPLMEVTKQEPPKPLRFLPGVLIGWENQPTTLELANKPLPVDDIL
                     QSLLGTTGQVYDQAQSVMEQNTVKEIPFLNEQTNSKIEKTDNVEVTDGENKEIKVKVDN
                     ISESTDKSAEIETSVVGSSSISAGSLTSLSLRGKPPDVSTEAFLTNLSIQSKQEETVES
                     KEKTLKRQLQEDQENNLQDNQTSNSSPCRSNVGKGNIDGNVSCSENLVANTARSPQFIN
                     LKRDPRQAAGRSQPVTTSESKDGDSCRNGEKHMLPGLSHNKEHLTEQINVEEKLCSAEK
                     NSCVQQSDNLKVAQNSPSVENIQTSQAEQAKPLQEDILMQNIETVHPFRRGSAVATSHF
                     EVGNTCPSEFPSKSITFTSRSTSPRTSTNFSPMRPQQPNLQHLKSSPPGFPFPGPPNFP
                     PQSMFGFPPHLPPPLLPPPGFGFAQNPMVPWPPVVHLPGQPQRMMGPLSQASRYIGPQN
                     FYQVKDIRRPERRHSDPWGRQDQQQLDRPFNRGKGDRQRFYSDSHHLKRERHEKEWEQE
                     SERHRRRDRSQDKDRDRKSREEGHKDKERARLSHGDRGTDGKASRDSRNVDKKPDKPKS
                     EDYEKDKEREKSKHREGEKDRDRYHKDRDHTDRTKSKR"
     misc_feature    2779..2784
                     /label=SDS
     misc_feature    3018..3023
                     /label=SDS
     CDS             3684..3749
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3753..4184
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3897..3902
                     /label=SDS
     repeat_region   4227..4367
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4227..4356
                     /label=AAV2 ITR
     repeat_region   4228..4368
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4442..4897
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5179..5283
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5284..6144
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6315..6903
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca ggttaaagat atgtacctta
     1501 ttcctttggg tgccacagat aaaattccac accctcttgt gccttttgat ggacctgggc
     1561 ttgaactgca tagacctaat ctattgttgg gcttaattat tcgtcagaaa ctgaagcgac
     1621 agcacagtgc ctgtgctagt actagtcata tagctgagac tcctgaaagt gcaccaccaa
     1681 tagcattgcc acctgataaa aaaagtaaaa tagaagtttc tacagaagaa gcaccagagg
     1741 aagaaaatga cttttttaat tcttttacaa ctgtattaca caagcagaga aataaacctc
     1801 agcagaatct tcaggaagac cttccaacag cagttgaacc tttaatggaa gtcaccaaac
     1861 aggagccacc aaaaccttta agatttcttc ctggcgtgtt gattggctgg gagaatcaac
     1921 ctactactct ggaattagca aataaacctc ttcctgtgga tgatatactt caaagccttt
     1981 tgggcaccac tggtcaagta tatgaccagg cccagtcagt gatggaacaa aacactgtta
     2041 aagaaattcc atttttaaat gagcagacca actcaaaaat agagaaaaca gataatgtgg
     2101 aagtaactga tggtgaaaac aaggagataa aagttaaagt agataatatt tcagaatcta
     2161 cagataagtc agcagaaata gaaacatcag tagtagggtc ctcttccatt tctgcagggt
     2221 ctttgacgag tcttagtctc agaggtaagc caccagatgt ttctacagaa gcatttttaa
     2281 caaatttatc aattcagtca aaacaagagg aaactgtgga gagtaaagag aaaacattaa
     2341 aaagacagct tcaggaagat caagagaata atttgcaaga taaccagact tcaaatagtt
     2401 ctccatgcag atctaatgta ggaaaaggaa acatagatgg taatgtgagc tgtagtgaaa
     2461 accttgttgc taatacagcg aggtctccac agtttatcaa cctgaaaagg gatcctaggc
     2521 aagcagcagg acgaagtcag cctgtaacta cttcagaaag caaagatgga gatagttgcc
     2581 ggaatggaga aaaacacatg ctgcctggcc tgtcacacaa caaggagcac ttaacagaac
     2641 aaatcaatgt agaggaaaag ttgtgttctg cagagaaaaa ctcgtgtgtt cagcagagtg
     2701 acaatttaaa agttgcacaa aactcaccat cagtagaaaa catacagact tctcaagcag
     2761 aacaagcaaa acccttacag gaggatattt taatgcaaaa tattgaaact gtgcacccat
     2821 ttcgaagagg atcagcagta gcgacatctc attttgaagt tggaaacaca tgtccatcag
     2881 aatttccttc taaaagcatc acctttactt ccagaagcac cagccccaga acaagtacaa
     2941 acttttcacc catgaggcca cagcagccca accttcagca tctcaagtct agcccacctg
     3001 gatttccatt tccagggcct cctaattttc ccccacaaag catgtttgga tttccaccac
     3061 atttgccacc tccattactt ccccctccag gctttggctt tgctcaaaat cccatggttc
     3121 cctggccacc tgttgttcat ctcccaggtc agccacagcg tatgatgggt cctctctcac
     3181 aagcatcaag gtatataggc ccgcagaatt tttaccaggt taaagacatt cggaggccag
     3241 aaaggcgcca tagtgaccct tggggtaggc aagaccaaca gcaactggat aggccattta
     3301 ataggggtaa aggggaccgc cagagatttt atagtgattc acaccatttg aaaagagagc
     3361 gacatgaaaa ggaatgggag caagaatctg aaaggcatag acgcagagac agaagccaag
     3421 acaaggacag agacagaaaa agcagggagg aagggcacaa agataaagag agggcacggt
     3481 tatcacatgg tgatcgagga acagatggaa aagcaagcag agatagtagg aatgtagaca
     3541 agaagccaga taaacctaaa agtgaagact atgagaagga caaagaacga gagaaaagta
     3601 aacacagaga aggagaaaag gacagggata ggtaccacaa agatagggac cacactgaca
     3661 gaactaaaag caaaaggtgt acagactaca aagaccatga cggtgattat aaagatcatg
     3721 acatcgacta caaggatgac gatgacaagt aactcgagat aatcaacctc tggattacaa
     3781 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     3841 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     3901 cttgtataaa tcctggttag ttcttgccac ggcggaactc atcgccgcct gccttgcccg
     3961 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttta tttgtgaaat
     4021 ttgtgatgct attgctttat ttgtaaccat ctagctttat ttgtgaaatt tgtgatgcta
     4081 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     4141 attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagatct ggagaccggt
     4201 gtggcacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc
     4261 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt
     4321 tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg
     4381 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag
     4441 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4501 gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4561 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4621 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
     4681 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4741 ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta
     4801 taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt
     4861 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc
     4921 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     4981 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     5041 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     5101 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     5161 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5221 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     5281 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     5341 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5401 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     5461 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     5521 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     5581 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5641 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     5701 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     5761 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     5821 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     5881 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5941 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     6001 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     6061 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     6121 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     6181 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6241 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6301 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6361 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6421 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     6481 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6541 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6601 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6661 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6721 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6781 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6841 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6901 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6961 atgt
//