| 质粒名称: | pAAV-hSyn-GRAB_NEmut |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000122 |
| 产品用途: | Expresses the NE-insensitive control sensor GRAB_NEmut in neurons |
| 存储实验室: | Yulong Li Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | hSyn |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | emiRFP670, HO1 |
| 物种来源: | Synthetic, Homo sapiens |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A Genetically Encoded Fluorescent Sensor for Rapid and Specific In Vivo Detection of Norepinephrine
Feng J., Zhang C., Lischinsky J.E., Jing M., Zhou J., Wang H., Zhang Y., Dong A., Wu Z., Wu H., Chen W., Zhang P., Zou J., Hires S., Zhu J., Cui G., Lin D., Du J., Li Y.
Neuron 102, 745-761.e8 (2019)
|
买家导航
pAAV-hSyn-GRAB_NEmut载体质粒基本信息
pAAV-hSyn-GRAB_NEmut质粒图谱载体图谱和pAAV-hSyn-GRAB_NEmut载体序列质粒序列多克隆位点信息
pAAV-hSyn-GRAB_NEmut质粒载体简介
pAAV-hSyn-GRAB_NEmut质粒序列载体序列
LOCUS pAAV-hSyn-GRAB_NEmut 6482 bp DNA circular SYN 21-APR-2023
DEFINITION Expresses the genetically-encoded fluorescent norepinephrine(NE)
control sensor GRAB_NEmut in neurons.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6482)
AUTHORS Feng J, Zhang C, Lischinsky JE, Jing M, Zhou J, Wang H, Zhang Y,
Dong A, Wu Z, Wu H, Chen W, Zhang P, Zou J, Hires SA, Zhu JJ, Cui G,
Lin D, Du J, Li Y
TITLE A Genetically Encoded Fluorescent Sensor for Rapid and Specific In
Vivo Detection of Norepinephrine.
JOURNAL Neuron. 2019 May 22;102(4):745-761.e8. doi:
10.1016/j.neuron.2019.02.037. Epub 2019 Mar 25.
PUBMED 30922875
REFERENCE 2 (bases 1 to 6482)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 27, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1016/j.neuron.2019.02.037"; journalName: "Neuron"; date:
"2019-05-22- 22"; volume: "102"; issue: "4"; pages: "745-761.e8"
FEATURES Location/Qualifiers
source 1..6482
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 68..197
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
promoter 198..641
/label=hSyn
primer_bind 547..566
/label=HSYN-F
regulatory 648..657
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
sig_peptide 654..716
/codon_start=1
/product="leader sequence from mouse immunoglobulin kappa
light chain"
/label=Ig-kappa leader
/translation="METDTLLLWVLLLWVPGSTGD"
protein_bind 717..741
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 741..2651
/codon_start=1
/label=GRAB_NEmut
/translation="TTGGSLQPDAGNASWNGTEAPGGGARATPYSLQVTLTLVCLAGLL
MLLTVFGNVLVIIAVFTSRALKAPQNLFLVSLASADILVATLVIPFSLANEVMGYWYFG
KAWCEIYLALDVLFCTSSIVHLCAISLDRYWSITQAIEYNLKRTPRRIKAIIITVWVIS
AVISFPPLISIEKKGGGGGPQPAEPRCEINDQKWYVISSCIGAFFAPCLIMILVYVRIY
QIAKRRTRVPPSRRGPDAVAAPPGGTERRPNGLGPERSAGPGGAEAEPLPTQLNGAPGE
PAPAGPRDTDALDLEEGGNVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIG
DGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVRK
GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVT
TLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVN
RIELKGIDFKEDGNILGHKLEYNTGAAARWRGRQNREKRFTFVLAVVIGVFVVCWFPFF
FTYTLTAVGCSVPRTLFKFFFWFGYCNSSLNPVIYTIFNHDFRRAFKKILCRGDRKRIV
L"
primer_bind 1330..1354
/label=WKG122-F
primer_bind 1844..1865
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(1971..1992)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2232..2251)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
misc_feature 2676..3264
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2729..2749)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3147..3158)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3296..3772
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind complement(3495..3514)
/label=hGH-PA-R
/note="Human growth hormone terminator, reverse primer"
repeat_region 3812..3952
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 3812..3941
/label=AAV2 ITR
rep_origin 4027..4482
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4114..4133)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4324..4345
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4499..4518)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4618..4640
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(4678..4696)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 4764..4868
/gene="bla"
/label=AmpR promoter
CDS 4869..5729
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5087..5106)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin join(5900..6482,1..6)
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6389..6408
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
61 cacatgtcct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg ggcgtcgggc
121 gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc
181 catcactagg ggttcctgcg gccgcacgcg tatgaggcgg ggtgggggtg cctacctgac
241 gaccgacccc gacccactgg acaagcaccc aacccccatt ccccaaattg cgcatcccct
301 atcagagagg gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc
361 accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga
421 aggcgcgctg acgtcactcg ccggtccccc gcaaactccc cttcccggcc accttggtcg
481 cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg aggcgcgaga taggggggca
541 cgggcgcgac catctgcgct gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg
601 cagcggagga gtcgtgtcgt gcctgagagc gcagtcgaga aaccggtgcc accatggaga
661 cagacacact cctgctatgg gtactgctgc tctgggttcc aggttccact ggtgacacaa
721 gtttgtacaa aaaagttggc accaccggtg gctccctgca gccggacgcg ggcaacgcga
781 gctggaacgg gaccgaggcg ccggggggcg gcgcccgggc caccccttac tccctgcagg
841 tgacgctgac gctggtgtgc ctggccggcc tgctcatgct gctcaccgtg ttcggcaacg
901 tgctcgtcat catcgccgtg ttcacgagcc gcgcgctcaa ggcgccccaa aacctcttcc
961 tggtgtctct ggcctcggcc gacatcctgg tggccacgct cgtcatccct ttctcgctgg
1021 ccaacgaggt catgggctac tggtacttcg gcaaggcttg gtgcgagatc tacctggcgc
1081 tcgacgtgct cttctgcacg tcgtccatcg tgcacctgtg cgccatcagc ctggaccgct
1141 actggtccat cacacaggcc atcgagtaca acctgaagcg cacgccgcgc cgcatcaagg
1201 ccatcatcat caccgtgtgg gtcatctcgg ccgtcatctc cttcccgccg ctcatctcca
1261 tcgagaagaa gggcggcggc ggcggcccgc agccggccga gccgcgctgc gagatcaacg
1321 accagaagtg gtacgtcatc tcgtcgtgca tcggcgcctt cttcgctccc tgcctcatca
1381 tgatcctggt ctacgtgcgc atctaccaga tcgccaagcg tcgcacccgc gtgccaccca
1441 gccgccgggg tccggacgcc gtcgccgcgc cgccgggggg caccgagcgc aggcccaacg
1501 gtctgggccc cgagcgcagc gcgggcccgg ggggcgcaga ggccgaaccg ctgcccaccc
1561 agctcaacgg cgcccctggc gagcccgcgc cggccgggcc gcgcgacacc gacgcgctgg
1621 acctggagga gggaggaaac gtctatatca aggccgacaa gcagaagaac ggcatcaagg
1681 cgaacttcca catccgccac aacatcgagg acggcggcgt gcagctcgcc taccactacc
1741 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagcg
1801 tgcagtccaa actttcgaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
1861 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggc ggtaccggag
1921 ggagcatggt gagaaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
1981 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggtgag ggcgatgcca
2041 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
2101 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
2161 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacatccag gagcgcacca
2221 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
2281 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
2341 ggcacaagct ggagtacaac accggagcag cagcacgctg gcgcgggcgg cagaaccgcg
2401 agaagcgctt cacgttcgtg ctggccgtgg tcatcggagt gttcgtggtg tgctggttcc
2461 ccttcttctt cacctacacg ctcacggccg tcgggtgctc cgtgccacgc acgctcttca
2521 aattcttctt ctggttcggc tactgcaaca gctcgttgaa cccggtcatc tacaccatct
2581 tcaaccacga tttccgccgc gccttcaaga agatcctctg tcggggggac aggaagcgga
2641 tcgtgttgtg agaattcgat atcaagctta tcgataatca acctctggat tacaaaattt
2701 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
2761 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
2821 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
2881 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
2941 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
3001 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
3061 tgtcggggaa atcatcgtcc tttccttggc tgctcgccta tgttgccacc tggattctgc
3121 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
3181 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
3241 tctccctttg ggccgcctcc ccgcatcgat accgagcgct gctcgagaga tctacgggtg
3301 gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc
3361 caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta
3421 taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg
3481 tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac
3541 tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg
3601 attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt
3661 caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc
3721 tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt
3781 gtaggtaacc acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact
3841 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
3901 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg
3961 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
4021 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
4081 tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc
4141 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
4201 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
4261 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
4321 atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg
4381 atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa
4441 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta
4501 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
4561 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
4621 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
4681 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
4741 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
4801 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
4861 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
4921 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
4981 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
5041 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
5101 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
5161 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
5221 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
5281 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
5341 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
5401 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
5461 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
5521 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
5581 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
5641 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
5701 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
5761 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
5821 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
5881 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
5941 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
6001 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
6061 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
6121 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
6181 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
6241 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
6301 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
6361 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
6421 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
6481 ta
//
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