pHAGE-CMV-dsRed-UBC-GFP-W

LOCUS       pHAGE-CMV-dsRed-UBC-GFP-W	8246 bp 	DNA	SYN
DEFINITION  pHAGE-CMV-dsRed-UBC-GFP-W
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8246
                     /organism="pHAGE-CMV-dsRed-UBC-GFP-W"
                     /mol_type="other DNA"
     misc_feature    454..634
                     /label="HIV-1_5_LTR"
     misc_feature    454..634
                     /label="truncHIV-1_3_LTR"
     misc_feature    745..789
                     /label="HIV-1_psi_pack"
     CDS             complement(1015..1476)
                     /label="ORF frame 3"
     CDS             1181..2107
                     /label="ORF frame 2"
     misc_feature    1303..1536
                     /label="RRE"
     misc_feature    2062..2077
                     /label="cPPT"
     promoter        2224..2728
                     /label="CMV_immearly_promoter"
     misc_feature    2231..2518
                     /label="CAG_enhancer"
     misc_feature    2685..2705
                     /label="CMV_fwd_primer"
     promoter        2686..2755
                     /label="CMV_promoter"
     misc_feature    2729..2748
                     /label="pCEP_fwd_primer"
     misc_feature    2731..2755
                     /label="LNCX_primer"
     gene            2812..3489
                     /label="DsRed_Express_2"
                     /gene="DsRed_Express_2"
     CDS             2812..3489
                     /label="ORF frame 1"
     gene            2815..3486
                     /label="DsRed_Express"
                     /gene="DsRed_Express"
     misc_feature    complement(2992..3012)
                     /label="dsRed1_N_primer"
     misc_feature    3404..3427
                     /label="dsRed1_C_primer"
     CDS             3903..4622
                     /label="ORF frame 3"
     gene            3906..4619
                     /label="EGFP"
                     /gene="EGFP"
     misc_feature    complement(3948..3969)
                     /label="EGFP_N_primer"
     misc_feature    4086..4115
                     /label="Y66 (EGFP)"
     misc_feature    4556..4577
                     /label="EGFP_C_primer"
     misc_feature    4638..5213
                     /label="WPRE"
     CDS             4726..5238
                     /label="ORF frame 1"
     CDS             4739..5272
                     /label="ORF frame 2"
     misc_feature    5282..5297
                     /label="cPPT"
     misc_feature    5282..5303
                     /label="U3PPT"
     misc_feature    5299..5351
                     /label="delta_U3"
     misc_feature    5352..5532
                     /label="HIV-1_5_LTR"
     misc_feature    5352..5532
                     /label="truncHIV-1_3_LTR"
     promoter        5772..5800
                     /label="AmpR_promoter"
     gene            5842..6702
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             5842..6702
                     /label="ORF frame 1"
     rep_origin      6857..7476
                     /label="pBR322_origin"
     misc_feature    7724..7743
                     /label="SV40pro_F_primer"
     promoter        7908..7937
                     /label="lac_promoter"
     misc_feature    7951..7973
                     /label="M13_pUC_rev_primer"
     misc_feature    8036..8055
                     /label="EBV_rev_primer"
ORIGIN
    1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
   61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
  121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
  181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
  241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
  301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
  361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
  421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
  481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
  541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
  601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
  661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
  721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
  781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
  841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
  901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
  961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
 2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
 2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
 2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
 2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
 2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
 2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
 2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
 2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
 2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
 2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccgc catggcctcc
 2821 tccgaggacg tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg ctccgtgaac
 2881 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
 2941 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
 3001 cagttccagt acggctccaa ggtgtacgtg aagcaccccg ccgacatccc cgactacaag
 3061 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
 3121 gtggtgaccg tgacccagga ctcctccctg caggacggct ccttcatcta caaggtgaag
 3181 ttcatcggcg tgaacttccc ctccgacggc cccgtaatgc agaagaagac tatgggctgg
 3241 gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccacaag
 3301 gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagtccat ctacatggcc
 3361 aagaagcccg tgcagctgcc cggctactac tacgtggact ccaagctgga catcacctcc
 3421 cacaacgagg actacaccat cgtggagcag tacgagcgcg ccgagggccg ccaccacctg
 3481 ttcctgtagg gatccggcct ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc
 3541 tcacggcgag cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc
 3601 ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc
 3661 agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga
 3721 gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg
 3781 gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc
 3841 agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggccgccc
 3901 atatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
 3961 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
 4021 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
 4081 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
 4141 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
 4201 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
 4261 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
 4321 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
 4381 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
 4441 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
 4501 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
 4561 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
 4621 aaatcgatag atcctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
 4681 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
 4741 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
 4801 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
 4861 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
 4921 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
 4981 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
 5041 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
 5101 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
 5161 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
 5221 ccgcctgaga tcctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
 5281 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
 5341 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
 5401 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
 5461 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
 5521 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
 5581 aatgaatatc agagagtgag aggcccgggt taattaagga aagggctaga tcattcttga
 5641 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
 5701 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
 5761 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
 5821 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
 5881 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
 5941 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
 6001 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
 6061 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
 6121 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
 6181 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
 6241 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
 6301 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
 6361 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
 6421 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
 6481 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
 6541 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
 6601 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
 6661 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
 6721 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
 6781 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
 6841 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
 6901 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
 6961 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
 7021 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
 7081 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
 7141 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
 7201 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
 7261 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
 7321 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
 7381 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
 7441 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
 7501 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
 7561 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
 7621 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
 7681 ccgattcatt aatgcagcaa gctcatggct gactaatttt ttttatttat gcagaggccg
 7741 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
 7801 gcttttgcaa aaagctcccc gtggcacgac aggtttcccg actggaaagc gggcagtgag
 7861 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
 7921 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
 7981 tatgacatga ttacgaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
 8041 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
 8101 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
 8161 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
 8221 taaatatgta ctacaaactt agtagt
//