| 质粒名称: | pAAV-CAG-mNeptune2.5 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000045 |
| 产品用途: | AAV, Expresses mNeptune2.5 in cytoplasm of mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a, TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | mNeptune2.5 |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Quantitative assessment of near-infrared fluorescent proteins
Zhang H., Papadaki S., Sun X., Wang X., Drobizhev M., Yao L., Rehbock M., Köster R.W., Wu L., Namikawa K., Piatkevich K.D.
Nat. Methods 2023 2010 20, 1605–1616 (2023)
|
pAAV-CAG-mNeptune2.5
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-CAG-mNeptune2.5载体质粒基本信息
pAAV-CAG-mNeptune2.5质粒图谱载体图谱和pAAV-CAG-mNeptune2.5载体序列质粒序列多克隆位点信息
pAAV-CAG-mNeptune2.5质粒载体简介
pAAV-CAG-mNeptune2.5质粒序列载体序列
LOCUS pAAV-CAG-mNeptune2.5 5458 bp DNA circular SYN 21-APR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5458)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Jun 24, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5458
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 279..556
/label=chicken beta-actin promoter
primer_bind 956..1000
/label=1108-mNeptune2.5-P2A-F
regulatory 977..982
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 983..1714
/codon_start=1
/product="brighter version of the far-red fluorescent
protein mNeptune2 with slightly blue-shifted spectra"
/label=mNeptune2.5
/translation="MVSKGEELIKENMHTKLYMEGTVNNHHFKCTHEGEGKPYEGTQTN
RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRCD
MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
ARYCDLPSKLGHKLNGMDELYK"
primer_bind complement(1694..1743)
/label=0503-mNeptune2.5-R
misc_feature 1742..2330
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(2213..2224)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 2373..2494
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3022..3610)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3781..4641)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4642..4746)
/gene="bla"
/label=AmpR promoter
rep_origin complement(4772..5227)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
61 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
241 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
301 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
361 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
421 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
481 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
541 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
601 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
661 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
721 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
781 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
841 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
901 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
961 aattggatcc ggtaccgcca ccatggtgag caagggcgag gagctgatca aggagaacat
1021 gcacacgaag ctgtacatgg aaggcaccgt gaacaaccac cacttcaagt gcacccacga
1081 aggggagggc aagccctacg agggcaccca gaccaacagg attaaggtgg tggagggagg
1141 ccccctgccg ttcgcattcg acatcctggc cacctgcttt atgtacggga gcaagacctt
1201 catcaaccac acccagggca tccccgattt ctttaagcag tccttccctg agggcttcac
1261 atgggagaga gtcaccacat acgaagacgg gggcgtgctt accgttaccc aggacaccag
1321 cctccaggac ggctgcttga tctacaacgt caagctcaga ggggtgaact tcccatccaa
1381 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc agtaccgaga ccctgtaccc
1441 cgctgacggc ggcctggaag gcagatgcga catggccctg aagctcgtgg gcgggggcca
1501 cctgcactgc aacctgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
1561 gcccggcgtc tactttgtgg accgcagact ggaaagaatc aaggaggccg acaatgagac
1621 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
1681 gcacaaactt aatggcatgg acgagctgta caagtaagaa ttcgatatca agcttatcga
1741 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
1801 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
1861 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
1921 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
1981 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
2041 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
2101 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
2161 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
2221 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
2281 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
2341 tcgacccggg cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac
2401 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
2461 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
2521 gtttcaggtt cagggggaga tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
2581 tggtaaaatc gataaggatc ttcctagagc atggctacgt agataagtag catggcgggt
2641 taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
2701 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
2761 cctcagtgag cgagcgagcg cgcagctgca ttaatgaatc ggccaacgcg cggggagagg
2821 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
2881 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
2941 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
3001 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
3061 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
3121 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
3181 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
3241 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
3301 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
3361 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
3421 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
3481 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
3541 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
3601 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
3661 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
3721 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
3781 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
3841 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
3901 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
3961 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
4021 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
4081 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
4141 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
4201 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
4261 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
4321 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
4381 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
4441 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
4501 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
4561 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
4621 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
4681 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
4741 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
4801 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
4861 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
4921 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
4981 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
5041 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
5101 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
5161 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
5221 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
5281 tcttcgctat tacgccagct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
5341 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
5401 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctact
//
pAAV-CAG-mNeptune2.5载体图谱质粒图谱pdf版和pAAV-CAG-mNeptune2.5载体序列质粒序列等相关资料下载
pAAV-CAG-mNeptune2.5质粒载体应用举例
pAAV-CAG-mNeptune2.5-P2A-GFP
2026-06-22
- 上一篇：pCytERM-miRFP720-N1
- 下一篇：没有了
