| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-RELN-C-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001738 |
| 产品用途: | 3' AAVLINK plasmid for RELN expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | RELN |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-RELN-C-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-RELN-C-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-RELN-C-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-RELN-C-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-RELN-C-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-RELN-C-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-RELN-C-FLAG 7417 bp DNA circular SYN 22-JUL-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7417)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7417
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..141
/label=AAV2 ITR
misc_feature 156..236
/label=SCP1
CDS 248..1276
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1292..1381
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1306..1311
/label=SDS
misc_feature 1309..1314
/label=SDS
intron 1385..1425
/label=Splicing Donor
protein_bind 1432..1465
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1466..1557
/label=Splicing Acceptor
/note="Splicing acceptor"
misc_feature 1558..4130
/label=RELN
misc_feature 1632..1637
/label=SDS
CDS 4137..4202
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 4206..4637
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4350..4355
/label=SDS
repeat_region 4680..4820
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4680..4809
/label=AAV2 ITR
rep_origin 4895..5350
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5632..5736
/gene="bla"
/label=AmpR promoter
CDS 5737..6597
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6768..7356
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtgtact tatataaggg ggtgggggcg
181 cgttcgtcct cagtcgcgat cgaacactcg agccgagcag acgtgcctac ggaccgcgta
241 cgccaccatg tccaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc
301 aacgagtgat gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc
361 tgagcatacc tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt
421 gaataaccgg aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct
481 tcaggcgcgc ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct
541 tcatcgtcgg tccgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg
601 gcggatccga aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga
661 acgcactgat ttcgaccagg ttcgttcact catggaaaat agcgatcgct gccaggatat
721 acgtaatctg gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc
781 caggatcagg gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg
841 cagaacgaaa acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac
901 taaactggtc gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct
961 gttttgccgg gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac
1021 tcgcgccctg gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga
1081 ctctggtcag agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga
1141 tatggcccgc gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt
1201 aaatattgta atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct
1261 gctggaagat ggcgatggag gtggatctgg tctgctgctg aaactcctcc tcctcggtgg
1321 tggttctggt cctcgtcccc gtggtactcg tggtaaaggt cgccgtattc actacaaagg
1381 ataagtaagt atcaaggtta caagacaggt ttaaggagac caataactag tataacttcg
1441 tataggatac tttatagcaa tttatgaaac tgggcttgtc gagacagaga agactcttgc
1501 gtttctgata ggcacctatt ggtcttactg acatccactt tgcctttctc tccacaggca
1561 ttacctggaa cctgctcatg gagattttct atgaccagta cagtaagccc ggatttgtga
1621 atatccttct ccctcctgat gctaaagaga ttgccactcg cttccgctgg tggcagccaa
1681 gacatgacgg cctggatcag aacgactggg ccattgacaa tgtcctcatc tcaggctctg
1741 ctgaccaaag gaccgttatg ctggacacct tcagcagcgc cccagtaccc cagcatgagc
1801 gctcccctgc agatgccggc cctgtcggga ggatcgcctt tgacatgttt atggaagaca
1861 aaacttcagt gaatgagcac tggctattcc atgatgattg tacagtagaa agattctgtg
1921 actcccctga tggtgtgatg ctctgtggca gtcatgatgg acgggaggtg tatgcagtga
1981 cccatgacct gactcccact gaaggctgga ttatgcaatt caagatctca gttggatgta
2041 aggtgtctga aaaaattgcc cagaatcaaa ttcatgtgca gtattctact gacttcggtg
2101 tgagttggaa ttatctggtc cctcagtgct tgcctgctga cccaaaatgc tctggaagtg
2161 tttctcagcc atctgtattc tttccaacta aagggtggaa aaggatcacc tacccacttc
2221 ctgaaagctt agtgggaaat ccggtaaggt ttaggttcta tcagaagtac tcagacatgc
2281 agtgggcaat cgataatttc tacctgggcc ctggatgctt ggacaactgc aggggccatg
2341 gagattgctt aagggaacag tgcatctgtg atccgggata ctcagggcca aactgctact
2401 tgacccacac tctgaagact ttcctgaagg aacgctttga cagtgaagaa atcaaacctg
2461 acttatggat gtccttagaa ggtggaagta cttgcactga gtgtggaatt cttgccgagg
2521 acactgcact ctattttggg ggatccactg tgagacaagc ggttacacaa gatttggatc
2581 ttcgaggtgc aaagttcctg caatactggg ggcgcatcgg tagtgagaac aacatgacct
2641 cttgccatcg tcccatctgc cggaaggaag gcgtgctgtt ggactactct accgatggag
2701 gaattacctg gactttgctc catgagatgg attaccagaa atacatttct gttagacacg
2761 actacatact tcttcctgaa gatgccctca ccaacacaac tcgacttcgc tggtggcagc
2821 cttttgtgat cagcaatgga attgtggtct ctggggtgga gcgtgctcag tgggcactgg
2881 acaacatttt gattggtgga gcagaaatca atcccagcca attggtggac acttttgatg
2941 atgaaggcac ttcccatgaa gaaaactgga gtttttaccc taatgctgta aggacagcag
3001 gattttgtgg caatccatcc tttcacctct attggccaaa taaaaagaag gacaagactc
3061 acaatgctct ctcctcccga gaactcatta tacagccagg atacatgatg cagtttaaaa
3121 ttgtggtggg ttgtgaagcc acttcttgtg gtgaccttca ttccgtaatg ctggaataca
3181 ctaaggatgc aagatcggat tcctggcagc tcgtacagac ccagtgcctt ccttcctctt
3241 ctaacagcat tggctgctcc cctttccagt tccatgaagc caccatctac aactctgtca
3301 acagctcaag ctggaaaaga atcaccatcc agctgcctga ccatgtctcc tctagtgcaa
3361 cacagttccg ctggatccag aagggagaag aaactgagaa gcaaagctgg gcaattgacc
3421 acgtgtacat tggagaggct tgccccaagc tctgcagcgg gcacggatac tgcacgaccg
3481 gtgccatctg catctgcgac gagagcttcc aaggtgatga ctgctctgtt ttcagtcacg
3541 accttcccag ttatattaaa gataattttg agtccgcaag agtcaccgag gcaaactggg
3601 agaccattca aggtggagtc ataggaagtg gctgtgggca gctggccccc tacgcccatg
3661 gagactcact gtactttaat ggctgtcaga tcaggcaagc agctaccaag cctctggatc
3721 tcactcgagc aagcaaaatc atgtttgttt tgcaaattgg gagcatgtcg cagacggaca
3781 gctgcaacag tgacctgagt ggcccccacg ctgtggacaa ggcagtgctg ctgcaataca
3841 gcgtcaacaa cgggatcacc tggcatgtca tcgcccagca ccagccaaag gacttcacac
3901 aagctcagag agtgtcttac aatgtccccc tggaggcacg gatgaaagga gtcttactgc
3961 gctggtggca accacgccac aatggaacag gtcatgatca atgggctttg gaccatgtgg
4021 aggtcgtcct agtaagcact cgcaaacaaa attacatgat gaatttttca cgacaacatg
4081 ggctcagaca tttctacaac agaagacgaa ggtcacttag gcgataccca tgtacagact
4141 acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca
4201 agtaactcga gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
4261 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
4321 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tagttcttgc
4381 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
4441 cactgacaat tccgtggtgt ttatttgtga aatttgtgat gctattgctt tatttgtaac
4501 catctagctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
4561 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga
4621 tgtgggaggt tttttaaaga tctggagacc ggtgtggcac gtgcggaccg agcggccgca
4681 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
4741 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
4801 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
4861 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
4921 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
4981 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
5041 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
5101 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
5161 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
5221 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
5281 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
5341 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
5401 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
5461 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
5521 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
5581 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
5641 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
5701 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
5761 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
5821 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
5881 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
5941 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
6001 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
6061 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
6121 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
6181 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
6241 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
6301 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
6361 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
6421 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
6481 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
6541 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
6601 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
6661 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
6721 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
6781 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
6841 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
6901 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
6961 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
7021 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
7081 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
7141 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
7201 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
7261 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
7321 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
7381 cggttcctgg ccttttgctg gccttttgct cacatgt
//
