pMx-puro-MGT

LOCUS       pMx-puro-MGT                9953 bp ds-DNA     circular SYN 23-FEB-2024
DEFINITION  Retroviral expression vector for direct cardiac reprogramming.
ACCESSION   .
VERSION     .
KEYWORDS    pMx-puro-MGT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9953)
  AUTHORS   Wang L, Liu Z, Yin C, Asfour H, Chen O, Li Y, Bursac N, Liu J, Qian 
            L
  TITLE     Stoichiometry of Gata4, Mef2c, and Tbx5 influences the efficiency 
            and quality of induced cardiac myocyte reprogramming.
  JOURNAL   Circ Res. 2015 Jan 16;116(2):237-44. doi: 
            10.1161/CIRCRESAHA.116.305547. Epub 2014 Nov 21.
  PUBMED    25416133
REFERENCE   2  (bases 1 to 9953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 23, 2024 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9953
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..594
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    657..1014
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1073..1489
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1419..1441
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1457..1473
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1499..1873
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1811..1827
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             3216..3272
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4617..4670
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(6267..6287)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6272..6601
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6452..6587
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6514..6533
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6611..7210
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6611..6630)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7107..7127
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7437..7906
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8036..8053)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8207..8795)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8287..8306)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8959..9819)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9582..9601
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9820..9924)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaaat acataactga gaatagaaaa gttcagatca aggtcaggaa cagatggaac
      121 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
      181 aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
      241 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct
      301 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
      361 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
      421 ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgattgact gagtcgcccg
      481 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
      541 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg
      601 ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc
      661 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc
      721 ctgcgtcggt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
      781 gagttcggaa cacccggccg caaccctggg agacgtccca gggacttcgg gggccgtttt
      841 tgtggcccga cctgagtcca aaaatcccga tcgttttgga ctctttggtg caccccccta
      901 ataggaggga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
      961 tgaatttttg ctttcggttt gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat
     1021 cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt agggccagac
     1081 tgttaccact cccttaagtt tgaccttagg tcactggaaa gatgtcgagc ggatcgctca
     1141 caaccagtcg gtagatgtca agaagagacg ttgggttacc ttctgctctg cagaatggcc
     1201 aacctttaac gtcggatggc cgcgagacgg cacctttaac cgagacctca tcacccaggt
     1261 taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc cctacatcgt
     1321 gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg tacaccctaa
     1381 gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc ctcgttcgac
     1441 cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccc ccatatggcc
     1501 atatgagatc ttatatgggg cacccccgcc ccttgtaaac ttccctgacc ctgacatgac
     1561 aagagttact aacagcccct ctctccaagc tcacttacag gctctctact tagtccagca
     1621 cgaagtctgg agacctctgg cggcagccta ccaagaacaa ctggaccgac cggtggtacc
     1681 tcacccttac cgagtcggcg acacagtgtg ggtccgccga caccagacta agaacctaga
     1741 acctcgctgg aaaggacctt acacagtcct gctgaccacc cccaccgccc tcaaagtaga
     1801 cggcatcgca gcttggatac acgccgccca cgtgaaggct gccgaccccg ggggtggacc
     1861 atcctctaga ctgccggatc tagctagtta attaaggatc cgccatgggg agaaaaaaga
     1921 ttcagattac gaggataatg gatgagcgta acagacaggt gacttttacg aagaggaaat
     1981 ttggattgat gaagaaggct tatgagctga gcgtgctgtg cgactgtgag attgcactga
     2041 tcatcttcaa cagcaccaac aagctgttcc agtacgccag cactgacatg gataaggtgt
     2101 tgctcaagta caccgagtac aacgagccgc acgagagccg gacaaactca gacattgtgg
     2161 aggcattgaa caagaaagaa aacaaaggct ctgaaagccc cgatcctgac tcctcttatg
     2221 cactcacccc acgcactgaa gaaaaataca aaaaaattaa tgaagaattt gataatatga
     2281 tcaagagtca taaaattcct gctgttccac ctcccagctt tgagatgcca gttaccatcc
     2341 cagtgtccag ccataacagt ttggtgtaca gcaatcctgt cagcacactg ggaaacccca
     2401 atcttctgcc actggcccac ccgtctctgc agaggaatag tatgtctcct ggtgtaacac
     2461 atagacctcc aagtgcaggt aacacaggcg gtctgatggg cggagatctg acatccggtg
     2521 caggcaccag cgcagggaat ggatacggca acccccggaa ctcaccaggc ctgctggtct
     2581 cacctggtaa cctgaacaag aatatacaag ccaaatctcc tccccctatg aatctaggaa
     2641 tgaataatcg taagccagat ctccgcgttc ttatcccacc tggcagcaag aacacgatgc
     2701 catcagtgaa tcaaaggata aataactccc agtcggctca gtcattggct accccggtgg
     2761 tttccgtagc aactcctact ttaccaggac aaggaatggg aggatatcca tcagccattt
     2821 caacaacata tggtactgag tactctctga gtagcgcaga tctgtcatct ctgtctggct
     2881 tcaacactgc cagtgcgctc cacctcggct ctgtaactgg ctggcagcag cagcacctac
     2941 ataacatgcc gccatctgcc ctcagtcagt tgggagaccg taccaccacc ccttcgagat
     3001 acccacaaca caccacgcgc cacgaggcgg ggaggtctcc tgttgacagc ttgagcagct
     3061 gtagcagttc ctacgatggg agcgaccgag aggatcaccg gaacgaattc cactccccca
     3121 ttggactcac cagaccttcg ccggacgaaa gggaaagtcc ttcagtcaag cgcatgcgac
     3181 tctctgaagg atgggcaaca gctagcggca gcggcgccac gaacttctct ctgttaaagc
     3241 aagcaggaga tgttgaagaa aaccccgggc ctgcatgcat gtaccaaagc ctggccatgg
     3301 ccgccaacca cggccccccg cccggcgcct acgaagcagg tggccctggc gccttcatgc
     3361 acagcgcggg cgccgcgtcc tcgcccgtct acgtgcccac tccgcgggtg ccgtcctctg
     3421 tgctgggcct gtcctacctg cagggcggtg gcagtgccgc tgcagctgga accacctcgg
     3481 gtggcagctc cggggccggc ccgtcgggtg cagggcctgg gacccagcag ggtagccctg
     3541 gctggagcca agctggagcc gagggagccg cctacacccc gccgcccgtg tccccgcgct
     3601 tctctttccc ggggactact gggtccctgg cggccgctgc cgccgctgcc gcagcccggg
     3661 aagctgcagc ctacggcagt ggcggcgggg cggcgggcgc tggtctggct ggccgagagc
     3721 agtacgggcg tccgggcttc gccggctcct actccagccc ctacccagcc tacatggccg
     3781 acgtgggagc atcctgggcc gcagccgctg ccgcctctgc cggccccttc gacagcccag
     3841 tcctgcacag cctgcctgga cgggccaacc ctggaagaca ccccaatctc gatatgtttg
     3901 atgacttctc agaaggcaga gagtgtgtca attgtggggc catgtccacc ccactctgga
     3961 ggcgagatgg gacgggacac tacctgtgca atgcctgtgg cctctatcac aagatgaacg
     4021 gcatcaaccg gcccctcatt aagcctcagc gccgcctgtc cgcttcccgc cgggtaggcc
     4081 tctcctgtgc caactgccag actaccacca ccacgctgtg gcgtcgtaat gccgagggtg
     4141 agcctgtatg taatgcctgc ggcctctaca tgaagctcca tggggttccc aggcctcttg
     4201 caatgcggaa ggaggggatt caaaccagaa aacggaagcc caagaacctg aataaatcta
     4261 agacgccagc aggtcctgct ggtgagaccc tccctccctc cagtggtgcc tccagcggta
     4321 actccagcaa tgccactagc agcagcagca gcagtgaaga gatgcgcccc atcaagacag
     4381 agcccgggct gtcatctcac tatgggcaca gcagctccat gtcccagaca ttcagtactg
     4441 tgtccggcca cgggccctcc atccatccag tgctgtctgc tctgaagctg tccccacaag
     4501 gctatgcatc tcctgtcact cagacatcgc aggccagctc caagcaggac tcttggaaca
     4561 gcctggtcct ggctgacagt catggggaca taatcaccgc ggcatgcggc tccggcgagg
     4621 gcaggggaag tcttctaaca tgcggggacg tggaggaaaa tcccggccca ctcgagatgg
     4681 ccgatacaga tgagggcttt ggcctggcgc gcacgcctct ggagcctgat tccaaagaca
     4741 ggtcttgcga ttcgaaacct gagagtgctc tgggggctcc cagcaagtct ccatcatccc
     4801 cgcaggctgc cttcacccag cagggcatgg aaggaatcaa ggtgtttctt catgaacgtg
     4861 aactgtggct gaagttccac gaagtgggca cagagatgat catcaccaag gcagggagga
     4921 gaatgtttcc tagttacaaa gtgaaggtga ctggccttaa tcccaaaacg aagtatattc
     4981 ttctcatgga tattgttccc gcagacgacc acagatataa atttgctgat aacaaatggt
     5041 ccgtaactgg caaagcagag cctgccatgc cggggcgcct ttacgtgcac ccggactccc
     5101 cagcaaccgg agcccactgg atgcgacaac ttgtctcctt ccagaagctc aaactcacca
     5161 acaaccacct ggacccgttt ggacacatta tcctgaactc catgcacaaa taccagcccc
     5221 gattacacat cgtgaaagca gacgaaaata atgggttcgg ttcaaagaac actgcgtttt
     5281 gcacccacgt cttcccggag acagctttta tcgctgtgac ttcgtaccag aatcacaaga
     5341 tcacacagct gaaaattgag aacaacccct tcgccaaagg ctttcggggc agtgatgacc
     5401 tggagttaca caggatgtct cggatgcaaa gtaaagagta tcctgtggtt cccaggagca
     5461 cagtgaggca caaagtcacc tccaaccaca gccccttcag cagcgagacc cgagctctct
     5521 ccacctcatc caatttaggg tcccagtacc agtgtgagaa tggtgtctct ggcccctccc
     5581 aggaccttct gcccccacct aacccatacc cactggccca ggagcacagc caaatttacc
     5641 actgtaccaa gaggaaagat gaggaatgtt ccagcacgga gcacccctat aagaagccgt
     5701 acatggagac atcccccagc gaggaagaca ccttctatcg ctcgggctac ccccagcagc
     5761 agggcctgag tacctcttac aggacagagt cggcccagcg gcaggcctgc atgtatgcca
     5821 gctccgctcc ccccagcgag cccgtgccta gcctggagga catcagctgt aacacatggc
     5881 ccagcatgcc ctcctatagc agctgtaccg tcaccaccgt gcagcccatg gaccgtcttc
     5941 cctaccagca cttctccgct catttcacct cggggcccct ggtccctcgg ttggctggca
     6001 tggccaacca tggttctccc cagctcggcg aagggatgtt tcagcaccag acctcagtgg
     6061 cccatcagcc tgtggtcagg cagtgcgggc ctcagactgg ccttcagtct ccgggcggcc
     6121 tccagccccc agagtttctc tacactcacg gcgtgcccag gaccctgtcc ccccatcagt
     6181 atcactcggt acacggcgtc ggcatggtgc cagagtggag tgagaatagc taagtcgagc
     6241 ggccgccagc acagtggtcg acctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     6301 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     6361 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     6421 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     6481 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6541 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     6601 aaagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     6661 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     6721 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     6781 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     6841 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     6901 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     6961 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     7021 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     7081 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     7141 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     7201 cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca
     7261 tgcatcgtcg acgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa
     7321 agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa
     7381 aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagctga
     7441 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
     7501 agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
     7561 ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa
     7621 ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact
     7681 aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa
     7741 gagcccacaa cccctcactc ggcgcgccag tcctccgatt gactgagtcg cccgggtacc
     7801 cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg
     7861 agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcacatg cagcatgtat
     7921 caaaattaat ttggtttttt ttcttaagta tttacattaa atggccatag ttgcattaat
     7981 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     8041 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8101 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8161 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8221 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8281 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8341 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8401 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8461 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8521 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8581 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8641 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8701 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8761 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8821 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8881 aaaggatctt cacctagatc cttttgcggc cggccgcaaa tcaatctaaa gtatatatga
     8941 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9001 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9061 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9121 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9181 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9241 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9301 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9361 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9421 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9481 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9541 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9601 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9661 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9721 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9781 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9841 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9901 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg cat
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