PCDH-CMV-mCherry-TRIM19

LOCUS       PCDH-CMV-mCherry-TRIM19              9798 bp DNA     circular SYN 23-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1904..1953
                     /label=Primer 4
     enhancer        1938..2241
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2242..2445
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2444..2491)
                     /label=Primer 1
     primer_bind     2466..2500
                     /label=Primer 2
     primer_bind     2466..2492
                     /label=Primer 5
     CDS             2496..3203
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3025..3046
                     /label=mcherry-F
     misc_feature    3204..3232
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     3220..3254
                     /label=TRIM19-F
     primer_bind     3220..3244
                     /label=TRIM18-F
     misc_feature    3240..5888
                     /label=TRIM19
     primer_bind     complement(5872..5908)
                     /label=TRIM19-R
     primer_bind     complement(5886..5908)
                     /label=TRIM18-R
     misc_feature    5895..6482
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6366..6377)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6555..6788
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6860..6981
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      7000..7135
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7173..7189)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7197..7213
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7221..7251)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7266..7287
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7575..8163)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8334..9194)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9195..9299)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9668..9681)
                     /label=Primer 5
     primer_bind     9773..9789
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagttgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2461 cagattctag accgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggata
     2521 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     2581 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     2641 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     2701 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     2761 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2821 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2881 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2941 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     3001 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     3061 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     3121 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     3181 gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcggaattca
     3241 tggagcctgc acccgcccga tctccgaggc cccagcagga ccccgcccgg ccccaggagc
     3301 ccaccatgcc tccccccgag accccctctg aaggccgcca gcccagcccc agccccagcc
     3361 ctacagagcg agcccccgct tcggaggagg agttccagtt tctgcgctgc cagcaatgcc
     3421 aggcggaagc caagtgcccg aagctgctgc cttgtctgca cacgctgtgc tcaggatgcc
     3481 tggaggcgtc gggcatgcag tgccccatct gccaggcgcc ctggccccta ggtgcagaca
     3541 cacccgccct ggataacgtc tttttcgaga gtctgcagcg gcgcctgtcg gtgtaccggc
     3601 agattgtgga tgcgcaggct gtgtgcaccc gctgcaaaga gtcggccgac ttctggtgct
     3661 ttgagtgcga gcagctcctc tgcgccaagt gcttcgaggc acaccagtgg ttcctcaagc
     3721 acgaggcccg gcccctagca gagctgcgca accagtcggt gcgtgagttc ctggacggca
     3781 cccgcaagac caacaacatc ttctgctcca accccaacca ccgcacccct acgctgacca
     3841 gcatctactg ccgaggatgt tccaagccgc tgtgctgctc gtgcgcgctc cttgacagca
     3901 gccacagtga gctcaagtgc gacatcagcg cagagatcca gcagcgacag gaggagctgg
     3961 acgccatgac gcaggcgctg caggagcagg atagtgcctt tggcgcggtt cacgcgcaga
     4021 tgcacgcggc cgtcggccag ctgggccgcg cgcgtgccga gaccgaggag ctgatccgcg
     4081 agcgcgtgcg ccaggtggta gctcacgtgc gggctcagga gcgcgagctg ctggaggctg
     4141 tggacgcgcg gtaccagcgc gactacgagg agatggccag tcggctgggc cgcctggatg
     4201 ctgtgctgca gcgcatccgc acgggcagcg cgctggtgca gaggatgaag tgctacgcct
     4261 cggaccagga ggtgctggac atgcacggtt tcctgcgcca ggcgctctgc cgcctgcgcc
     4321 aggaggagcc ccagagcctg caagctgccg tgcgcaccga tggcttcgac gagttcaagg
     4381 tgcgcctgca ggacctcagc tcttgcatca cccaggggaa agatgcagct gtatccaaga
     4441 aagccagccc agaggctgcc agcactccca gggaccctat tgacgttgac ctgcccgagg
     4501 aggcagagag agtgaaggcc caggttcagg ccctggggct ggctgaagcc cagcctatgg
     4561 ctgtggtaca gtcagtgccc ggggcacacc ccgtgccagt gtacgccttc tccatcaaag
     4621 gcccttccta tggagaggat gtctccaata caacgacagc ccagaagagg aagtgcagcc
     4681 agacccagtg ccccaggaag gtcatcaaga tggagtctga ggaggggaag gaggcaaggt
     4741 tggctcggag ctccccggag cagcccaggc ccagcacctc caaggcagtc tcaccacccc
     4801 acctggatgg accgcctagc cccaggagcc ccgtcatagg aagtgaggtc ttcctgccca
     4861 acagcaacca cgtggccagt ggcgccgggg aggcagagga acgcgttgtg gtgatcagca
     4921 gctcggaaga ctcagatgcc gaaaactcgt cctcccgaga gctggatgac agcagcagtg
     4981 agtccagtga cctccagctg gaaggcccca gcaccctcag ggtcctggac gagaaccttg
     5041 ctgaccccca agcagaagac agacctctgg ttttctttga cctcaagatt gacaatgaaa
     5101 cccagaagat tagccagctg gctgcggtga accgggaaag caagttccgc gtggtcatcc
     5161 agcctgaagc cttcttcagc atctactcca aggccgtgtc cctggaggtg gggctgcagc
     5221 acttcctcag ctttctgagc tccatgcgcc gccctatctt ggcctgctac aagctgtggg
     5281 ggcctggcct cccaaacttc ttccgggccc tggaggacat taacaggctg tgggaattcc
     5341 aggaggccat ctcgggcttc ctggctgccc tgcctctcat ccgggagcgt gtgcccgggg
     5401 ccagcagctt caaactcaag aacctggccc agacctacct ggcgagaaac atgagcgagc
     5461 gcagcgccat ggctgccgtg ctggccatgc gtgacctgtg ccgcctcctc gaggtctccc
     5521 cgggccccca gctggcccag catgtctacc ccttcagtag cctgcagtgc tttgcctccc
     5581 tgcagcccct ggtgcaggca gctgtgctgc cccgggctga ggcccgcctc ctggccctac
     5641 acaacgtgag cttcatggag ctgctgagtg cacaccgccg tgaccggcag gggggcctga
     5701 agaagtacag ccgctatcta agcctgcaga ccaccacgtt gccccctgcc cagcctgctt
     5761 tcaacctgca ggctctgggc acctactttg aaggcctgtt ggagggtccg gcgctggcac
     5821 gggcagaagg agtctccacc ccacttgctg gccgtggctt ggcagagagg gcctcccagc
     5881 agagctgagg atccaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     5941 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6001 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6061 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6121 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6181 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6241 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     6301 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6361 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6421 ctcttccgcg tctccgcctt cgccctcaga cgagtcggat ctccctttgg ccgcctcccc
     6481 gccggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     6541 agaaaagggg ggactggaag ggctaattca ctcccaacga aaataagatc tgctttttgc
     6601 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6661 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     6721 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     6781 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     6841 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     6901 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6961 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     7021 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7081 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac
     7141 ttttgcagag acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     7201 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     7261 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     7321 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7381 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7441 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7501 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7561 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7621 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7681 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7741 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7801 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7861 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7921 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7981 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     8041 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     8101 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     8161 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     8221 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     8281 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8341 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8401 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8461 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8521 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8581 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8641 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8701 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8761 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8821 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8881 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8941 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     9001 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     9061 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     9121 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     9181 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     9241 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9301 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     9361 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     9421 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     9481 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     9541 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     9601 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
     9661 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     9721 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     9781 acggccagtg ccaagctg
//